scholarly journals Antioxidant activity, α-glucosidase inhibition and phytochemical profiling of Hyophorbe lagenicaulis leaf extracts

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7022 ◽  
Author(s):  
James William ◽  
Peter John ◽  
Muhammad Waseem Mumtaz ◽  
Ayoub Rashid Ch ◽  
Ahmad Adnan ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Synthetic Drugs ◽  
Promising Source ◽  
Phenolic And Flavonoid ◽  
Tof Ms

Background Diabetes mellitus type II (DMT-2) is a widely spread metabolic disorder both in developed and developing countries. The role of oxidative stress is well established in DMT-2 pathogenesis. The synthetic drugs for DMT-2 are associated with serious side complications. Antioxidant and α-glucosidase inhibitory actions of phytochemicals from various plant species are considered as an alternative to synthetic drugs for DMT-2 management. The present study aimed to evaluate the antioxidant activity, α-glucosidase inhibitory potential and phytochemical profiling of Hyophorbe lagenicaulis. Methods The total phenolic and flavonoid contents, in vitro antioxidant activity (α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging and phosphomolybdenum method) and α-glucosidase inhibition of ultrasonicated hydroethanolic H. lagenicaulis leaf extracts were determined spectrophotometrically. The results of DPPH assay and α-glucosidase inhibition were reported in terms of IC50 value. The phytochemical profiling was accomplished by UHPLC-Q-TOF/MS/MS technique. Results and Discussion Findings leaped 60% ethanolic extract as rich fraction regarding total phenolic and flavonoid contents. The 60% ethanolic fraction was a promising source of natural antioxidants and α-glucosidase inhibitory agents as indicated by anti-radical and enzyme inibitory activities. Kaempferol, rutin, hesperetin 5-O-glucoside, kaempferol-coumaroyl-glucoside, luteolin 3-glucoside, Isorhamnetin-3-O-rutinoside, trimethoxyflavone derivatives and citric acid were identified by UHPLC-Q-TOF-MS/MS. These compounds were believed to be responsible for the strong antioxidant and enzyme inhibitory activity of plant extracts. The extensive metabolite profiling of H. lagenicaulis was carried out the first time as never reported previously. The H. lagenicaulis might be an appropriate choice to manage diabetes mellitus in an alternate way. The findings may be further exploited extensively for toxicity evaluation to proceed with functional food development having antidiabetic attributes.

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY OF VARIOUS LEAF EXTRACTS OF CANTHIUM COROMANDELICUM(BURM.F.) ALSTON.

2017 ◽  
Vol 10 (5) ◽  
pp. 214
Author(s):  
R.shabi Ruskin
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Peroxide Radical ◽  
Leaf Extracts ◽  
Phenolic And Flavonoid ◽  
In Vitro Antioxidant Activity

Objectives: The objective of this study was to estimate the total phenolic and flavonoid contents and to explore the antioxidant potential of various leaf extracts (chloroform, ethyl acetate, and ethanol) of Canthium coromandelicum, which is considered traditionally as an important medicinal plant.Methods: Antioxidant properties of the extracts were assessed using 1, 1- diphenyl -2- picrylhydrazyl and hydrogen peroxide radical scavenging assays for ascorbic acid equivalents. The total phenolic and flavonoid contents were also investigated to determine their correlation with the antioxidant activity of the leaf extracts and expressed in Gallic acid and quercetin equivalents, respectively.Results: The results showed that the content of total phenols and flavonoids was found to be high in ethyl acetate extract which was recorded as 61.02±1.30 mg Gallic acid equivalent (GAE/g) and 81.72±0.61 QE/g, respectively. Compared to other extracts, ethyl acetate leaf extract was found to possess high antioxidant activity at p<0.05 level, with high percentage of inhibition at 100 μg/ml concentration (82.70%) toward hydrogen peroxide radical scavenging with IC50 value 62.94 μg/ml. Statistically, two-tailed Pearson’s correlation showed strong positive correlations between hydrogenperoxide radical scavenging activity and total phenolic contents (TPC) (r=1.000) at p<0.05 level.Conclusion: The results obtained in this study clearly signifies that the ethyl acetate leaf extract of C. coromandelicum has high content of total phenols which are correlated to its antioxidant activity and thus has the potential to use as a source of natural antioxidants and can be explored as a therapeutic agent in free radical induced diseases.Keywords: Canthium coromandelicum, In-vitro antioxidant activity, Ethyl acetate extract, Pearson’s correlation, 1, 1- diphenyl -2- picrylhydrazyl, Hydrogen peroxide, Nitric oxide, Superoxide.

scholarly journals Evaluation of Free Radical Scavenging and Polyphenolic Contents of Bark of Pterygota alata Roxb

2014 ◽  
Vol 6 (3) ◽  
pp. 543-552 ◽  
Author(s):  
N. Jahan ◽  
M. S. Parvin ◽  
A. Khan ◽  
N. Das ◽  
M. S. Islam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Soluble Fraction ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Deciduous Tree ◽  
Total Phenolic ◽  
Phenolic And Flavonoid

Pterygota alata is a large deciduous tree of Malvaceae family. The present study was designed to evaluate in vitro antioxidant activity of stem bark of the plant. Ferric reducing power (FRP) test, 1, 1- diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging test and total antioxidant capacity (TAC) assay were used to detect the antioxidant activity. Total phenolic and flavonoid contents were also determined as they are well known phytochemicals with antioxidant property. The bark of the plant showed efficient reducing power as well as free radical scavenging property (IC50 values 52.25 - 172.05). The bark also found rich in total phenolic and flavonoid content. The highest amount of total phenolic content was found in chloroform soluble fraction (29.898 µg/mL) followed by ethyl acetate soluble fraction (15.88 µg/mL). The highest content of total flavonoid also detected in chloroform soluble fraction (107.56 µg/mL) but followed by crude ethanol extract (98.66 µg/mL). Overall, the bark of the plant possesses significant antioxidant activity, therefore can be used as a good natural source of antioxidant. © 2014 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi: http://dx.doi.org/10.3329/jsr.v6i3.17586 J. Sci. Res. 6 (3), 543-552 (2014)

scholarly journals Antioxidant activity and total phenolic and flavonoid contents of Salvia amplexicaulis Lam. extracts

2014 ◽  
Vol 66 (1) ◽  
pp. 307-316 ◽  
Author(s):  
Ana Alimpic ◽  
Mariana Oaldje ◽  
V. Matevski ◽  
P.D. Marin ◽  
Sonja Duletic-Lausevic
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Methanol Extracts ◽  
Whole Plant ◽  
Phenolic And Flavonoid

This study was designed to examine the in vitro antioxidant DPPH free radical-scavenging activity, and the total phenolic and flavonoid content of ethanol and methanol extracts of Salvia amplexicaulis Lam. in the whole plant and different parts, leaves, stems and flowers. The largest amounts of extract yield were obtained from the flowers, 14.14% and 12.00 % (w/w) in the ethanol of methanol extracts, respectively. The ethanol extract of leaves (16.07 ?g/ml) and methanol extract of the whole plant (21.28 ?g/ml) showed the highest activity against the DPPH radical. The ethanol extract of the leaves was the richest in phenols (222.40 mg GAE/g) and flavonoids (49.81 mg QE/g), whereas the methanol extract of the whole plant contained the highest amount of phenolics (180.89 mg GAE/g) and flavonoids (38.15 mg QE/g). A very strong linear correlation between antioxidant activity and the phenolic content of the extracts was established. The obtained results suggest that S. amplexicaulis could be regarded as an important source of natural antioxidants.

scholarly journals In vitro free radical scavenging and antidiabetic activity of aqueous and ethanolic leaf extracts: a comparative evaluation of Argyreia pierreana and Matelea denticulata

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Venkataiah Gudise ◽  
Bimalendu Chowdhury ◽  
Arehalli S. Manjappa
Keyword(s):  
Glucose Uptake ◽  
Radical Scavenging ◽  
Vascular Complications ◽  
Total Phenolic ◽  
Aqueous Extracts ◽  
Leaf Extracts ◽  
Glut 4 ◽  
Ethanolic Extracts ◽  
Phenolic And Flavonoid

Abstract Background Oxidation is believed to play a vital role in the pathogenesis of diabetes mellitus by lipid peroxidation; DNA and protein damage leads to the development of vascular complications like coronary heart disease, stroke, neuropathy, retinopathy, and nephropathy. The herbal preparations are complementary and alternative medicines to allopathic drugs which are believed to cause adverse events. Therefore, the current study was aimed to identify the novel plants, which belong to the genera Argyreia (Argyreia pierreana (AP)) and Matelea (Matelea denticulata (MD)), and assess the aqueous and ethanolic leaf extracts for in vitro antioxidant and antidiabetic potential by DPPH, OH•, superoxide, and glucose uptake and gene expression (GLUT-4 and PPARγ) studies using the L-6 cell line respectively. Results The preliminary scrutiny revealed the presence of polyphenols, flavonoids, terpenoids, steroids, tannins, alkaloids, and glycosides. The total phenolic and flavonoid contents of ethanolic extracts were found higher than those of aqueous extracts. The ethanolic extracts exhibited the superior antioxidant capacity when compared with aqueous extracts. However, the ethanolic extract of MD was shown superlative glucose uptake activity (72.54%) over control (0.037%) and GLUT-4 and PPARγ gene expressions (1.17 and 1.20) in term of folds respectively over cell control (1.00). Conclusion The ethanolic leaf extracts of both plants showed significant in vitro antioxidant and antidiabetic activities compare to aqueous extracts. The Matelea denticulata ethanolic leaf extract exhibited superior activity. This superior activity might be due to their higher phenolic and flavonoid content. However, further approaches are needed to define these activities.

scholarly journals Quantification of phenolics, flavonoids and antioxidant activity of Tamarindus indica from selected areas in Tanzania

2018 ◽  
Vol 16 (1) ◽  
pp. 22-28
Author(s):  
MOURICE MBUNDE ◽  
ROBINSON H. MDEGELA ◽  
H.S. LASWAI ◽  
F.P. MABIKI
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Tamarindus Indica ◽  
Leaf Extracts ◽  
Fruit Extracts ◽  
Significant Difference ◽  
Phenolic And Flavonoid ◽  
Quantification Of Phenolics

Mbunde M, Mdegela RH, Laswai HS, Mabiki FP. 2018. Quantification of phenolics, flavonoids and antioxidant activity of Tamarindus indica from selected areas in Tanzania. Biofarmasi J Nat Prod Biochem15: 22-28. The objective of this study was to establish the quantities and antioxidant activity in fruits and leaves of Tamarindus indica L. collected from three agro-ecological zones of Tanzania represented by Morogoro, Tanga and Dodoma regions. Samples were examined for their total phenolic and flavonoid contents as well as their antioxidant activity. The total phenolic content showed significant difference in all extracts of the fruits and leaves and ranged from 1994.4±530.77 to 17874.67±5234 mg GAE/100 g. Similarly, the total flavonoid content in tamarind leaf and fruit extracts ranged from 880±609.45 to 11483.11±2559.67 mg CE /100 g dry weight. There was a significant difference between the antioxidant activity in the leaf (54.39±0.13%) and fruit extracts (40.11±0.03%). Tamarind leaf extracts exhibited significantly higher radical scavenging activity than fruit extracts. The antioxidant activity in fruit extracts expressed in percentage ranged between 29.27±0.06% and 40.11±0.03% while in leaf extracts the activity ranged from 22.33±0.08% to 54.39±0.13%. The radical scavenging activity from Coastal leaf extracts had the highest activity followed by Eastern leaf extracts and lastly Central leaf extracts. In the fruit samples, the highest activity was shown by Coastal fruit extracts followed by Central fruit extracts and Eastern fruit extract being the least active. The values in Ferric reducing power (FRAP) assay ranged between 6968±3655.91 µM Fe (II)/g and 76822.67±23259.9 µM Fe (II)/g for leaves and fruits dry mass respectively. These values correspond to the antioxidant activity which positively correlated with the total phenolic and flavonoid contents. Geographical location and climatic conditions have been reported to have enormous effects on the amount and activity of antioxidants available in both tamarind leaves and fruits. Findings from the study indicated that tamarind can be utilized as a cheap source of antioxidants. However, more agronomic studies should be considered to confirm the effects of agroecological differences on antioxidant activity.

scholarly journals Antioxidant activity and total phenolic and flavonoid contents of Chrysopogon aucheri (Boiss.) Stapf

2017 ◽  
Vol 63 (2) ◽  
pp. 42-47
Author(s):  
Maryam Sadoughinia ◽  
Jinous Asgarpanah
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Aluminium Chloride ◽  
Biological Research ◽  
Total Phenolic ◽  
Total Extract ◽  
Phenolic And Flavonoid

Summary Introduction: Chrysopogon aucheri is a species native to the South of Iran. It is used for the treatment of some oxidative-based disorders. Objective: Due to lack of biological research on C. aucheri, we were prompted to investigate the antioxidant activities of leaf total extract and different sub-fractions for the first time. The phenolic and flavonoid contents were also determined in the leaves as the interaction between these components and the antioxidant activity. Methods: The antioxidant effects of total extract and sub-fractions were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. Total phenolic and flavonoid contents were also determined by Folin-Ciocalteu and aluminium chloride colorimetric methods, respectively. Results: Total extract of C. aucheri leaves was found to possess desirable antioxidant activity in vitro in comparison with standard antioxidant BHT (butylated hydroxytoluene). The highest phenolic and flavonoid contents were observed in the methanol sub-fraction. Results showed weak and moderate correlations with phenolics and flavonoids, respectively, and therefore other metabolites of C. aucheri leaves might be responsible for antioxidant activities. Conclusion: The results suggested that C. aucheri leaves possess desirable antioxidant activity when compared with BHT and support the ethnomedicinal claims of the use of the leaves in the management of some oxidative-based diseases.

scholarly journals In vitro antioxidant activity of dewberry (Rubus caesius L. var. aquáticus Weihe. & Nees.) leaf extracts

2015 ◽  
Vol 67 (4) ◽  
pp. 1323-1330 ◽  
Author(s):  
Ivona Velickovic ◽  
Slavica Grujic ◽  
Ana Dzamic ◽  
Zoran Krivosej ◽  
Petar Marin
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Acetone Extract ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Leaf Extracts ◽  
Reducing Ability ◽  
Reducing Capacity

In this study, the antioxidant properties and total phenolic and flavonoid contents of Rubus caesius L. var. aqu?ticus Weihe & Nees leaf extracts were examined. The radical scavenging capacity of four leaf extracts (methanol, ethanol, acetone and aqueous) was estimated against 2,2-diphenyl-1-picrylhydrazil (DPPH) and 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) assays. Total antioxidant capacity was tested by ferric reducing ability of plasma (FRAP) and total reducing capacity (TRC) procedures. Total contents of phenols and flavonoids were also determined. Total phenol content in the extracts was determined using Folin-Ciocalteu reagent and amounts ranged between 263.74 for ethanol to 366.27 mg gallic acid (GA)/g for acetone extracts. The amounts of flavonoids varied from 10.73 mg quercetin equivalent (QE)/g for aqueous to 75.83 mg QE/g for acetone extract. The highest antioxidant activity against DPPH and ABTS?+ radicals was that of the acetone extract. Values for FRAP varied between 3.00 ?mol Fe+2/mg for the ethanol extract and 5.31 ?mol Fe+2/mg for the methanol extract. The results obtained for total reducing capacity indicate that the acetone extract has the highest antioxidant potential.

scholarly journals Honeybee Pollen Extracts Reduce Oxidative Stress and Steatosis in Hepatic Cells

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 6
Author(s):  
Juan Esteban Oyarzún ◽  
Marcelo E. Andia ◽  
Sergio Uribe ◽  
Paula Núñez Pizarro ◽  
Gabriel Núñez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Cell Model ◽  
Radical Scavenging ◽  
Central Zone ◽  
Total Phenolic ◽  
Nonalcoholic Fatty Liver ◽  
Bee Pollen ◽  
Quercetin Concentration

Nonalcoholic fatty liver disease (NAFLD) is a major cause of morbidity and mortality worldwide. Additional therapies using functional foods and dietary supplements have been investigated and used in clinical practice, showing them to be beneficial. Honeybee pollen from Chile has shown a large concentration of phenolic compounds and high antioxidant activity. In this work, we characterized twenty-eight bee pollen extracts from the central zone of Chile according to botanical origin, phenolic profile, quercetin concentration, and antioxidant activity (FRAP and ORAC-FL). Our results show a statistically significant positive correlation between total phenolic content and antioxidant capacity. Selected samples were evaluated on the ability to reverse the steatosis in an in vitro cell model using Hepa1-6 cells. The pollen extracts protected Hepa1-6 cells against oxidative damage triggered by 2,2′-azo-bis(2-amidinopropane) dihydrochloride (AAPH)derived free radicals. This effect can be credited to the ability of the phenolic compounds present in the extract to protect the liver cells from chemical-induced injury, which might be correlated to their free radical scavenging potential. Additionally, bee pollen extracts reduce lipid accumulation in a cellular model of steatosis. In summary, our results support the antioxidant, hepatoprotective, and anti-steatosis effect of bee pollen in an in vitro model.

scholarly journals IN VITRO - ANTIOXIDANT AND PRELIMINARY PHYTOCHEMICAL ANALYSIS OF AEGLE MARMELOS (L.) CORREA. LEAF EXTRACT

2017 ◽  
Vol 10 (6) ◽  
pp. 367
Author(s):  
Samidha M Pawaskar ◽  
Sasangan Kc
Keyword(s):  
Hydroxyl Radical ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Aegle Marmelos ◽  
Leaf Powder ◽  
Phenolic And Flavonoid

Objective: In this study, the leaf powder of Aegle marmelos (L.) Correa. was subjected to preliminary phytochemical and in vitro antioxidant analysis. Methods: The freshly prepared plant leaf extract was subjected to preliminary phytochemical screening, which revealed the presence of alkaloids, tannins, saponins, flavonoids, glycosides, phenolic compounds, terpenoids, and steroids. Reducing power, superoxide (SO) anion radical, nitric oxide (NO) radical, and hydroxyl radical scavenging assays were carried out to evaluate the antioxidant potential of the methanolic leaf extract of this plant. The amounts of total phenolic and flavonoid compounds were also determined. Results: This study has revealed that the A. marmelos (L.) Correa. leaf extract showed considerably high amounts of most of the phytochemicals, total antioxidant capacity, total phenolic, and flavonoid content. The study also indicated that the A. marmelos (L.) Correa. showed comparatively good scavenging activity, i.e., inhibition of hydroxyl radical, NO and SO anion scavenging and reducing power activities when compared with the respective standards. Conclusion: The leaf powder of A. marmelos (L.) Correa. can be used as easily accessible source of natural antioxidant and as a possible food supplement or in pharmaceutical industry.

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