scholarly journals Optimization of extraction conditions and determination of purine content in marine fish during boiling

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6690 ◽  
Author(s):  
Tingting Li ◽  
Likun Ren ◽  
Dangfeng Wang ◽  
Minjie Song ◽  
Qiuying Li ◽  
...  
Keyword(s):  
Marine Fish ◽  
High Performance ◽  
Scophthalmus Maximus ◽  
Abdominal Muscles ◽  
Time Saving ◽  
Purine Bases ◽  
Solid Ratio ◽  
Mixed Acid ◽  
Purine Content ◽  
Edible Parts

Background Gout is the second most common metabolic disease affecting human health. The disease of gout is closely related to the level of uric acid, which is the end-product of human purine metabolism. Moreover, food is the main way of external ingestion of purine. Method A simple and time-saving method was developed to extract purines like adenine, hypoxanthine, guanine, and xanthine from marine fish by single factor design combined with Box–Behnken. The contents of these purines in the edible parts and internal organs of marine fish, as well as Scophthalmus maximus, were determined by high-performance liquid chromatography to investigate the relationship between the boiling process and purine content. Result The mixed-acid method was chosen for the extraction of purine bases and the extraction conditions were as follows: mixture acid 90.00% TFA/80.00% FA (v/v, 1:1); hydrolysis temperature 90.00 °C; time 10.00 min; liquid-to-solid ratio 30:1. The total purine content of the edible parts (eyes, dorsal muscles, abdominal muscles, and skin) was the highest in Scophthalmus maximus, followed by sphyraena, Sardinella, Trichiurus lepturus, Scomberomorus niphonius, Pleuronectiformes, Sea catfish, Anguillidae, and Rajiformes. Moreover, boiling significantly reduced the purine content in the marine fish because of the transfer of the purines to the cooking liquid during boiling. Scophthalmus maximus, Sphyraena, and Sardinella were regard as high-purine marine fish, which we should eat less. We also confirmed that boiling significantly transferred purine bases from fish to cooking liquid. Thus, boiling could reduce the purine content of fish, thereby reducing the risk of hyperuricemia and gout.

Determination of the Isoflavone Genistein in Soybeans by High-Performance Liquid Chromatography Following Cloud Point Extraction

2012 ◽  
Vol 95 (3) ◽  
pp. 845-849 ◽  
Author(s):  
Mohammad Mirzaei ◽  
Atieh Karimi Naeini ◽  
Mansoureh Behzadi
Keyword(s):  
Cloud Point ◽  
High Performance ◽  
Cloud Point Extraction ◽  
Uv Detector ◽  
Experimental Conditions ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Replicate Measurements

Abstract A simple and sensitive method has been developed for preconcentration and determination of genistein in soybeans. This method is based on cloud point extraction (CPE) of genistein from soybeans using ethylene glycol monoalkyl ether (Genapol X-080) as a nonionic surfactant. The concentration of extracted genistein was determined by HPLC with a UV detector. Optimum experimental conditions were established. With 5% Genapol X-080 (v/v), a liquid/solid ratio of 25:1 mL/g, and ultrasonic-assisted extraction at 40°C for 45 min, the extraction percentage of genistein reached its highest value. The preconcentration factor for genistein was about 16.5. The RSD for seven replicate measurements and the LOD were ±4.45% and 15.0 ng/mL, respectively. CPE is simple, inexpensive, and suitable for extraction of genistein from soybean. It uses environmentally friendly surfactants and offers a convenient alternative to more conventional extraction systems.

scholarly journals Total purine content and comparison of the ratio of purine bases in common foodstuffs

2015 ◽  
Vol 39 (1) ◽  
pp. 7-21 ◽  
Author(s):  
Kiyoko Kaneko ◽  
Tomoko Fukuuchi ◽  
Katsunori Inazawa ◽  
Noriko Yamaoka ◽  
Shin Fujimori
Keyword(s):  
Purine Bases ◽  
Purine Content

scholarly journals CuO nanorods grown vertically on graphene nanosheets as a battery-type material for high-performance supercapacitor electrodes

RSC Advances ◽  
2020 ◽  
Vol 10 (60) ◽  
pp. 36554-36561
Author(s):  
Miaomiao Zhai ◽  
Ang Li ◽  
Jingbo Hu
Keyword(s):  
High Performance ◽  
Graphene Nanosheets ◽  
Type Material ◽  
Rate Performance ◽  
Time Saving ◽  
Cuo Nanorods

The CuO/rGO@NF electrode is prepared by a simple and time-saving method, which has ultrahigh area capacity and excellent rate performance.

High-Performance Liquid Chromatography Determination of Acrylamide after Its Extraction from Potato Chips

2019 ◽  
Vol 25 (4) ◽  
pp. 338-344
Author(s):  
Maryam Ghalebi ◽  
Samin Hamidi ◽  
Mahboob Nemati
Keyword(s):  
Organic Solvent ◽  
Organic Phase ◽  
High Performance ◽  
Single Step ◽  
Potato Chips ◽  
Process Efficiency ◽  
Local Market ◽  
Good Precision ◽  
Time Saving ◽  
Relative Standard

Background: Acrylamide is a known carcinogenic product that has been found among the substances such as potato chips which to be processed under the heat-treatment. In order to extract amounts of acrylamide from fried chips in market, an ultrasound-assisted liquid– liquid extraction (UA-LLE) technique is proposed. The UA-LLE coupled LLE and ultrasonication in a single step. Methods: Chips samples were dissolved in an extracting organic solvent using ultrasonication to prompt transferring of acrylamide into the organic phase. As a result, the extraction time and process efficiency were significantly enhanced through increasing the collision power and mass transfer between grounded chips and organic phase. Results: Important parameters affecting the extraction efficiency such as kind of organic solvent and its volume, re-dissolving solvent and pH were optimized. This newly proposed method has been applied to determine the trace acrylamide in potato chips samples purchased from local market. Conclusion: UA-LLE is a handy, economic and time-saving method, with high extraction yield (over 103% average recovery) and good precision (lower than 15% relative standard deviation, RSD). Most importantly, it seems this method to be an ideal pre-treatment method for the extraction of acrylamide in food matrix in food quality control laboratories.

scholarly journals Application of Emerging Cell Disintegration Techniques for the Accelerated Recovery of Curcuminoids from Curcuma longa

2021 ◽  
Vol 11 (17) ◽  
pp. 8238
Author(s):  
Hoang Le-Tan ◽  
Thomas Fauster ◽  
Jelena Vladic ◽  
Tina Gerhardt ◽  
Klara Haas ◽  
...  
Keyword(s):  
Specific Energy ◽  
High Performance ◽  
Energy Input ◽  
Curcuma Longa ◽  
Time Saving ◽  
Specific Energy Input ◽  
Beneficial Effects ◽  
High Specific Energy ◽  
The Impact ◽  
Cell Disintegration

Curcuminoids, the bioactive compounds with many beneficial effects on human health, exist in Curcuma longa (turmeric). In the present study, the impact of different cell disintegration techniques to enhance total curcuminoid recovery (TC) from fresh and dried turmeric was investigated. The impact of thermal pretreatment (TP), ultrasound pretreatment (UP), enzyme pretreatment (EP), and pulsed electric field pretreatment (PEF) on the recovery of curcumin (CUR), demethoxycurcumin (DMC), and bis-demethoxycurcumin (BDMC) from fresh and dried turmeric were studied. The cell disintegration index (Zp) and high-performance liquid chromatography (HPLC) analysis of curcuminoids were performed to evaluate the efficiency of the applied techniques. With fresh turmeric, the highest curcuminoid recovery was 83.6 mg/g dry basis with EP. The highest structural tissue damage was obtained with UP achieving a cell disintegration level of 92.5%. The technology with the highest time-saving and low specific energy input was PEF with a total curcuminoid recovery of 80.9 mg/g dry basis. Working with dried turmeric, the drying required high specific energy input for 72 h at 50 °C; however, the untreated dried sample reached 125.3 mg/g dry basis of TC without further pretreatment after drying.

scholarly journals Enhancement of Solasodine Extracted from Fruits of Solanum nigrum L. by Microwave-Assisted Aqueous Two-Phase Extraction and Analysis by High-Performance Liquid Chromatography

Molecules ◽  
2019 ◽  
Vol 24 (12) ◽  
pp. 2294 ◽  
Author(s):  
Li Lin ◽  
Wen Yang ◽  
Xing Wei ◽  
Yi Wang ◽  
Li Zhang ◽  
...  
Keyword(s):  
Extraction Method ◽  
High Performance ◽  
Extraction Yield ◽  
Solanum Nigrum ◽  
Two Phase ◽  
Solid Ratio ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Solanum Nigrum L ◽  
Aqueous Two Phase Extraction

Background: Solasodine is a major bioactive ingredient in Solanum nigrum L. that has strong pharmacological characteristics. Therefore, the development of a simple and effective extraction method for obtaining solasodine is highly important. This study aims to provide a rapid and effective method for extracting solasodine from Solanum nigrum L. by microwave-assisted aqueous two-phase extraction (MAATPE). Methods: First, the high-performance liquid chromatography (HPLC) conditions were established for the detection of solasodine. Then, the aqueous two-phase system (ATPS) compositions were examined. On the basis of the results of single-factor experiments, for a better yield, response surface methodology (RSM) was used to optimize influential factors including the extraction temperature, extraction time and liquid-to-solid ratio. Results: The maximum extraction yield of 7.11 ± 0.08 mg/g was obtained at 44 °C, an extraction time of 15 min, and a liquid-to-solid ratio of 42:1 mL/g in the ATPS consisting of EtOH solvent, (NH4)2SO4, and water (28:16:56, w/w/w). The extraction yield of the alkaloid obtained using this method was markedly higher than those of microwave-assisted extraction (MAE) and ultrasonic-assisted extraction (UAE). Conclusions: In this work, solasodine was extracted by MAATPE for the first time and a high yield was obtained. MAATPE is a simple, rapid, and green technique for extraction from medical plants. Thus, the present study will enable the development of a feasible extraction method of active alkaloids from Solanum nigrum L.

scholarly journals Polyphenolic Profile and Varied Bioactivities of Processed Taiwanese Grown Broccoli: A Comparative Study of Edible and Non-Edible Parts

2020 ◽  
Vol 13 (5) ◽  
pp. 82 ◽  
Author(s):  
Thanh Ninh Le ◽  
Napat Sakulsataporn ◽  
Chiu-Hsia Chiu ◽  
Pao-Chuan Hsieh
Keyword(s):  
High Performance ◽  
Inhibitory Concentration ◽  
Antioxidant Activities ◽  
Antibacterial Properties ◽  
Pharmaceutical Products ◽  
Health Promoting ◽  
Weak Activity ◽  
Promising Source ◽  
Edible Parts

Broccoli contains a substantial amount of bioactive compounds such as glucosinolates, phenolics, and essential nutrients, which are positively linked to health-promoting effects. This work aimed to evaluate whether both edible and non-edible parts of broccoli could be effective by examining in vitro antioxidant, cytotoxic, apoptotic, and antibacterial properties of its floret, leaf, and seed extracts (FE, LE, and SE, correspondingly). High-performance liquid chromatography (HPLC) and various assays exhibited strong antioxidant activities of all samples. LE obtained the highest capacity, correlated to its polyphenolic contents. SE exerted significant cytotoxicity against A549, Caco-2, and HepG2 cancer cell lines at low inhibitory concentration (IC)50 values (0.134, 0.209, and 0.238 mg/mL, respectively), as tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Flow cytometry confirmed apoptosis induction of extracts in Caco-2 cells by revealing an increased subG1 population and a decreased mitochondrial membrane potential. The considerable antibacterial efficacy was observed in either LE and SE against Bacillus subtilis and Salmonella typhimurium (0.39–0.78 mg/mL) using well-agar diffusion and minimum inhibitory concentration (MIC) techniques, along with the weak activity against Staphylococcus aureus and Escherichia coli (1.56–3.13 mg/mL). The findings suggest that broccoli and its byproducts might serve as a promising source for further development of food or pharmaceutical products.

Sign in / Sign up

Export Citation Format

Share Document