scholarly journals Synthesis of light-inducible and light-independent anthocyanins regulated by specific genes in grape ‘Marselan’ (V. vinifera L.)

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6521 ◽  
Author(s):  
Zong-Huan Ma ◽  
Wen-Fang Li ◽  
Juan Mao ◽  
Wei Li ◽  
Cun-Wu Zuo ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Total Concentration ◽  
Gene Expression Profiles ◽  
Grape Berry ◽  
Light Transmittance ◽  
Anthocyanin Synthesis ◽  
Berry Skin ◽  
Total Anthocyanin ◽  
Regulation Mechanisms ◽  
Bhlh Transcription Factors

Anthocyanin is an important parameter for evaluating the quality of wine grapes. However, the effects of different light intensities on anthocyanin synthesis in grape berry skin and its regulation mechanisms are still unclear. In this experiment, clusters of wine grape cv. ‘Marselan’ were bagged using fruit bags with different light transmittance of 50%, 15%, 5%, and 0, designated as treatment A, B, C and D, respectively. Fruits that were not bagged were used as the control, designated as CK. The anthocyanin composition and concentration, as well as gene expression profiles in the berry skin were determined. The results showed that the degree of coloration of the berry skin reduced with the decrease of the light transmittance, and the veraison was postponed for 10 days in D when compared with the CK. Total anthocyanin concentration in the berry skin treated with D decreased by 51.50% compared with CK at the harvest stage. A total of 24 and 21 anthocyanins were detected in CK and D, respectively. Among them, Malvidin-3-O-coumaroylglucoside (trans), which showed a significant positive correlation with the total concentration of anthocyanins at the harvest stage (r = 0.775) and was not detected in D, was presumed to be light-induced anthocyanin. Other anthocyanins which were both synthesized in CK and D were considered to be light-independent anthocyanins. Among them, Malvidin-3-O-coumaroylglucoside (cis) and Malvidin-3-O-acetylglucoside were typical representatives. Remarkably, the synthesis of light-inducible anthocyanins and light-independent anthocyanins were regulated by different candidate structural genes involved in flavonoid biosynthesis pathway and members of MYB and bHLH transcription factors.

scholarly journals Nitrogen and phosphorus co-starvation inhibits anthocyanin synthesis in the callus of grape berry skin

2020 ◽  
Vol 142 (2) ◽  
pp. 313-325 ◽  
Author(s):  
Hui-Zhen Zheng ◽  
Huan Wei ◽  
Shui-Huan Guo ◽  
Xin Yang ◽  
Ming-Xin Feng ◽  
...  
Keyword(s):  
Grape Berry ◽  
Anthocyanin Synthesis ◽  
Nitrogen And Phosphorus ◽  
Berry Skin

scholarly journals Transcriptome analysis identified genes involved in anthocyanin biosynthesis in Rainbow bamboo (Indosasa hispida MeClure cv. ‘Rainbow’)

Plant Omics ◽  
2018 ◽  
pp. 145-152
Author(s):  
Yi Wang ◽  
YuMing Yang ◽  
Juan Wang ◽  
Yuan XiaoLong
Keyword(s):  
Transcriptome Analysis ◽  
Anthocyanin Biosynthesis ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Anthocyanin Synthesis ◽  
Expression Levels ◽  
Dihydroflavonol Reductase ◽  
Qrt Pcr ◽  
Genes Encoding ◽  
And Function

Rainbow bamboo (Indosasa hispida) is an ornamental plant, which contains unique red to purple anthocyanin in its culm. However, the biosynthesis and function of anthocyanin in bamboo remains unclear. In this study, RNA-seq was used to investigate the transcriptome of the species and compare the gene expression profiles of red and white culms. The expression levels of genes involved in the anthocyanin biosynthesis pathway were detected using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). In total, 5.92 billion reads were obtained from the culm of Rainbow bamboo, which were assembled into 60,716 unigenes. qRT-PCR showed that the expression levels of anthocyanin biosynthesis-related genes in the red and white culms were higher than that in green leaves and that their levels in the red culm without sheath were higher than that in the white culm with sheath. Transcriptome analysis and qRT-PCR showed that the differences in the expression of genes encoding chalcone isomerase (CHI), dihydroflavonol reductase (DFR), flavonoid 3'-hydroxylase (F3'H), and anthocyanidin 3-O-glycosyltransferase (A3GT) between the culm and leaf were significant. This implies that CHI, DFR, F3'H, and A3GT play important roles in anthocyanin synthesis and accumulation in the culm of Rainbow bamboo.

scholarly journals Leaf:fruit Ratio Affects the Proteomic Profile of Grape Berry Skins

2013 ◽  
Vol 138 (6) ◽  
pp. 416-427 ◽  
Author(s):  
Ben-Hong Wu ◽  
Ning Niu ◽  
Ji-Hu Li ◽  
Shao-Hua Li
Keyword(s):  
Anthocyanin Biosynthesis ◽  
Titratable Acidity ◽  
Grape Berry ◽  
Soluble Solids ◽  
Anthocyanin Synthesis ◽  
Berry Skin ◽  
Two Dimensional Gel Electrophoresis ◽  
Dihydroflavonol Reductase ◽  
Cell Defense ◽  
Berry Skins

The most obvious effects of a low leaf:fruit (LF) ratio [two leaves for one cluster per shoot (LF2)] on grape (Vitis vinifera) berries are suppressed anthocyanin biosynthesis in the berry skin, decreased berry weight and soluble solids concentration, and increased titratable acidity. In this study, proteins isolated from berry skins grown under low and high LF ratio conditions, LF2 and LF12, respectively, were characterized by two-dimensional gel electrophoresis coupled to mass spectrometry. A survey of ≈600 to 700 spots from berry skin yielded 77 proteins with differential expression between LF12 and LF2 treatments. Of these, the 59 proteins that were identified consisted of 47 proteins that were down-regulated and 12 that were up-regulated under LF2 conditions compared with LF12 conditions. Most proteins involved in metabolism, energy, transcription, protein synthesis, binding function, signal transduction, and cell defense were down-regulated in LF2 berries, whereas two important enzymes of anthocyanin biosynthesis, chalcone synthase and dihydroflavonol reductase, were not detected. Only a few proteins (e.g., two heat shock proteins related to protein fate and nutrient reservoir storage protein) were found to be up-regulated in LF2 berries. This suggested that, with the exception of secondary metabolism, many proteomic events may have an effect on anthocyanin synthesis in the skins responding to LF.

scholarly journals Genome-wide characterization and analysis of bHLH transcription factors related to anthocyanin biosynthesis in spine grapes (Vitis davidii)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ming Li ◽  
Lei Sun ◽  
Hong Gu ◽  
Dawei Cheng ◽  
XiZhi Guo ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Transcription Factor ◽  
Transcription Factors ◽  
Candidate Genes ◽  
Grape Berry ◽  
Synthesis Process ◽  
Anthocyanin Synthesis ◽  
Bhlh Transcription Factor ◽  
Transcription Factor Family ◽  
Bhlh Transcription Factors

AbstractAs one of the largest transcription factor family, basic helix-loop-helix (bHLH) transcription factor family plays an important role in plant metabolism, physiology and growth. Berry color is one of the important factors that determine grape quality. However, the bHLH transcription factor family’s function in anthocyanin synthesis of grape berry has not been studied systematically. We identified 115 bHLH transcription factors in grape genome and phylogenetic analysis indicated that bHLH family could be classified into 25 subfamilies. First, we screened six candidate genes by bioinformatics analysis and expression analysis. We found one of the candidate genes VdbHLH037 belonged to III (f) subfamily and interacted with genes related to anthocyanin synthesis through phylogenetic analysis and interaction network prediction. Therefore, we speculated that VdbHLH037 participated in the anthocyanin synthesis process. To confirm this, we transiently expressed VdbHLH037 in grape and Arabidopsis transformation. Compared with the control, transgenic materials can accumulate more anthocyanins. These results provide a good base to study the function of the VdbHLH family in anthocyanin synthesis of grape berry.

scholarly journals Variability in cellular gene expression profiles and homeostatic regulation

2015 ◽  
Author(s):  
Yuriy Mishchenko
Keyword(s):  
Gene Expression ◽  
Protein Expression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Homeostatic Regulation ◽  
Cellular Gene ◽  
Large Variability ◽  
Gene And Protein Expression ◽  
Regulation Mechanisms ◽  
Protein Expression Profiles

One of surprising recent discoveries in biology is that the gene and protein expression profiles in cells with identical genetic and environmental makeup can exhibit large variability. The nature and the significance of this variability had been posed as one of the current fundamental questions in biology. In this letter, we argue that the observed variability in cellular gene and protein expression can be understood as an outcome of homeostatic regulation mechanisms controlling the gene and protein expression profiles.

scholarly journals Phenolic compounds and antioxidant capacities in grape berry skin, seed and stems of six winegrape varieties grown in Turkey

2021 ◽  
Vol 20 (1) ◽  
pp. 15-25
Author(s):  
Hande Tahmaz Karaman ◽  
Damla Yüksel Küskü ◽  
Gökhan Söylemezoğlu
Keyword(s):  
Phenolic Compounds ◽  
Red Wine ◽  
Grape Berry ◽  
Total Phenolic ◽  
Cabernet Sauvignon ◽  
Wine Grape ◽  
Berry Skin ◽  
Total Anthocyanin ◽  
Total Phenolic Compound ◽  
Grape Varieties

In this study, seeds, skins and stems of the red wine grape varieties Boğazkere, Cabernet Sauvignon, Merlot, Nero d’Avola, Sangiovese and Syrah grown in Turkey were analysed for their phenolic compounds. The highest total phenolic compound and DPPH were found in the stem of Boğazkere respectively as 62550 mg GAE/kg dw and 614 µmol/g dw; the highest ABTS•+, (+)-catechin, (–)-epicatechin were detected in the seed of Nero d’Avola respectively as 617 µmol trolox/g dw, 8650 mg/kg and 1902 mg/kg dw; the highest total anthocyanin and rutin were measured in the skin of Boğazkere respectively as 143.52 mg/kg dw and 9692 mg/kg dw; the highest quercetin was found in the seed of Boğazkere as 49.21 mg/kg dw and the highest trans-resveratrol was measured in the stem of Syrah as 61.56 mg/kg dw.

1327: Gene Expression Profiles in Benign Prostatic Hyperplasia

2004 ◽  
Vol 171 (4S) ◽  
pp. 349-350
Author(s):  
Gaelle Fromont ◽  
Michel Vidaud ◽  
Alain Latil ◽  
Guy Vallancien ◽  
Pierre Validire ◽  
...  
Keyword(s):  
Gene Expression ◽  
Benign Prostatic Hyperplasia ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Prostatic Hyperplasia
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