scholarly journals Mycorrhization of Quercus acutissima with Chinese black truffle significantly altered the host physiology and root-associated microbiomes

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6421 ◽  
Author(s):  
Xiaoping Zhang ◽  
Lei Ye ◽  
Zongjing Kang ◽  
Jie Zou ◽  
Xiaoping Zhang ◽  
...  
Keyword(s):  
Plant Physiology ◽  
Soil Properties ◽  
Host Plant ◽  
Community Composition ◽  
Rhizosphere Soil ◽  
Early Stage ◽  
Microbial Community Composition ◽  
Symbiotic Relationship ◽  
Black Truffle ◽  
Quercus Acutissima

Background Our aim was to explore how the ectomycorrhizae of an indigenous tree,Quercus acutissima, with a commercial truffle, Chinese black truffle (Tuber indicum), affects the host plant physiology and shapes the associated microbial communities in the surrounding environment during the early stage of symbiosis. Methods To achieve this, changes in root morphology and microscopic characteristics, plant physiology indices, and the rhizosphere soil properties were investigated when six-month-old ectomycorrhizae were synthesized. Meanwhile, next-generation sequencing technology was used to analyze the bacterial and fungal communities in the root endosphere and rhizosphere soil inoculated with T. indicum or not. Results The results showed that colonization by T. indicum significantly improved the activity of superoxide dismutase in roots but significantly decreased the root activity. The biomass, leaf chlorophyll content and root peroxidase activity did not obviously differ. Ectomycorrhization of Q. acutissima with T. indicum affected the characteristics of the rhizosphere soil, improving the content of organic matter, total nitrogen, total phosphorus and available nitrogen. The bacterial and fungal community composition in the root endosphere and rhizosphere soil was altered by T. indicum colonization, as was the community richness and diversity. The dominant bacteria in all the samples were Proteobacteria and Actinobacteria, and the dominant fungi were Eukaryota_norank, Ascomycota, and Mucoromycota. Some bacterial communities, such as Streptomyces, SM1A02, and Rhizomicrobium were more abundant in the ectomycorrhizae or ectomycorrhizosphere soil. Tuber was the second-most abundant fungal genus, and Fusarium was present at lower amounts in the inoculated samples. Discussion Overall, the symbiotic relationship between Q. acutissima and T. indicum had an obvious effect on host plant physiology, soil properties, and microbial community composition in the root endosphere and rhizosphere soil, which could improve our understanding of the symbiotic relationship between Q. acutissima and T. indicum, and may contribute to the cultivation of truffle.

Volatile-mediated suppression of plant pathogens is related to soil properties and microbial community composition

2018 ◽  
Vol 117 ◽  
pp. 164-174 ◽  
Author(s):  
Maaike van Agtmaal ◽  
Angela L. Straathof ◽  
Aad Termorshuizen ◽  
Bart Lievens ◽  
Ellis Hoffland ◽  
...  
Keyword(s):  
Microbial Community ◽  
Soil Properties ◽  
Community Composition ◽  
Plant Pathogens ◽  
Microbial Community Composition

scholarly journals High-methionine soybean has no significant effect on nitrogen-transforming bacteria in rhizosphere soil

2018 ◽  
Vol 64 (No. 3) ◽  
pp. 108-113 ◽  
Author(s):  
Liang Jingang ◽  
Luan Ying ◽  
Jiao Yue ◽  
Sun Shi ◽  
Wu Cunxiang ◽  
...  
Keyword(s):  
Community Composition ◽  
Rhizosphere Soil ◽  
Microbial Community Composition ◽  
Potential Effect ◽  
Ammonia Oxidizing Bacteria ◽  
Nitrogen Fixing ◽  
Transgenic Soybean ◽  
Bacterial Populations ◽  
Microbial Functional Groups ◽  
Microbial Groups

Transgenic plants may induce shifts in the microbial community composition that in turn alter microbially-mediated nutrient cycling in soil. Studies of how specific microbial groups respond to genetically modified (GM) planting help predict potential impacts upon processes performed by these groups. This study investigated the effect of transgenic high-methionine soybean cv. ZD91 on nitrogen-fixing and ammonia-oxidizing bacterial populations. A difference in nitrogen-fixing or ammonia-oxidizing bacteria community composition was not found, suggesting that cv. ZD91 does not alter the bacterial populations in rhizosphere soil. This study increases our understanding of the potential effect of transgenic soybean on microbial functional groups within soil by suggesting that nitrogen-transforming bacteria may be useful for future investigations on the GM crops impact in the soil ecosystem.

scholarly journals Soil Microbial Community Composition and Diversity Remained Unchanged in a Semiarid Grassland in Northwestern China After 7 Years of Nitrogen Addition

2021 ◽  
Author(s):  
Qian Guo ◽  
Zhongming Wen ◽  
Hossein Ghanizadeh ◽  
Cheng Zheng ◽  
Yongming Fan ◽  
...  
Keyword(s):  
Microbial Community ◽  
Soil Properties ◽  
Community Composition ◽  
Microbial Community Composition ◽  
N Deposition ◽  
Leaf Nitrogen Content ◽  
N Addition ◽  
Weighted Mean ◽  
Soil Microbial ◽  
Community Weighted Mean

Abstract Aims Nitrogen (N) deposition is a global environmental problem that can alter community compositions and functions, and consequently, the ecosystem services. In this study, we assessed the responses of aboveground vegetation, surface soil properties and microbial communities to N addition, and explored the drivers of microbial community in a semiarid steppe ecosystem in northwest of China. Methods Thirty-six 6×10-m2 plots composed of six N addition levels and six replicates were distributed in six columns and six rows. Nine vegetation characteristics and seven soil properties were measured and calculated. Soil microbial characteristics were analyzed by 16S rRNA high-throughput sequencing. Results N addition positively affected aboveground vegetation traits such as the community weighted-mean of leaf nitrogen content (LNCWM). High N inputs significantly altered the microbial community assembly process from random to deterministic. The microbial community diversity and composition, however, were not sensitive to N addition. A piecewise structural equation model (SEM) further showed that the microbial community composition was affected by both aboveground vegetation and soil properties. The composition of bacterial communities was mainly regulated by the composition of plant communities and soil total N. In contrast, the composition of fungal communities was driven by soil pH and the community weighted-mean of specific leaf area (SLACWM). Microbial diversity and composition remained unchanged because their drivers were not affected by N addition. The results of this research improved our understanding of the response of grassland ecosystems to N deposition, and provided a theoretical basis for grassland utilization and management under N deposition.

scholarly journals Maize phyllosphere microbial community niche development across stages of host leaf growth

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 1698
Author(s):  
Heather C. Manching ◽  
Kara Carlson ◽  
Sean Kosowsky ◽  
C. Tyler Smitherman ◽  
Ann E. Stapleton
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Host Plant ◽  
Community Composition ◽  
Leaf Growth ◽  
Abiotic Factors ◽  
Microbial Community Composition ◽  
Electron Microscopic ◽  
Positive Role ◽  
Spacer Length

Background: The phyllosphere hosts a variety of microorganisms, including bacteria, which can play a positive role in the success of the host plant. Bacterial communities in the phylloplane are influenced by both biotic and abiotic factors, including host plant surface topography and chemistry, which change in concert with microbial communities as the plant leaves develop and age.Methods: We examined how theZea maysL. leaf microbial community structure changed with plant age. Ribosomal spacer length and scanning electron microscopic imaging strategies were used to assess microbial community composition across maize plant ages, using a novel staggered experimental design.Results: Significant changes in community composition were observed for both molecular and imaging analyses, and the two analysis methods provided complementary information about bacterial community structure within each leaf developmental stage.Conclusions: Both taxonomic and cell-size trait patterns provided evidence for niche-based contributions to microbial community development on leaves.

scholarly journals Effects of sodium citrate on the structure and microbial community composition of an early-stage multispecies biofilm model

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yuan Yao ◽  
Yang Pu ◽  
Wing Yui Ngan ◽  
Karin Kan ◽  
Jie Pan ◽  
...  
Keyword(s):  
Microbial Community ◽  
Carbon Source ◽  
Community Composition ◽  
Nutrient Medium ◽  
Sodium Citrate ◽  
Early Stage ◽  
Microbial Community Composition ◽  
Biofilm Model ◽  
Multispecies Biofilm ◽  
Biofilm Development

Abstract In recent years, most biofilm studies have focused on fundamental investigations using multispecies biofilm models developed preferentially in simulated naturally occurring low-nutrient medium than in artificial nutrient-rich medium. Because biofilm development under low-nutrient growth media is slow, natural media are often supplemented with an additional carbon source to increase the rate of biofilm formation. However, there are knowledge gaps in interpreting the effects of such supplementation on the resulting biofilm in terms of structure and microbial community composition. We investigated the effects of supplementation of a simulated freshwater medium with sodium citrate on the resulting structure, bacterial community composition, and microbial network interactions of an early-stage multispecies biofilm model. Qualitative and quantitative analyses of acquired confocal laser scanning microscopy data confirmed that sodium citrate supplementation distinctly increased biofilm biomass. Sequencing data revealed that the microbial community structure of biofilms grown in sodium citrate-supplemented conditions was characterized with increased relative abundance and dominance of Proteobacteria compared with that of biofilms grown in sodium citrate-free conditions. Our findings suggest that the supplementation of a low-nutrient medium with a carbon source in experiments involving multispecies biofilms may lead to structural and compositional biases of the microbial community, causing changes in biofilm phenotype.

scholarly journals Soil microbial community composition in a paddy field with different fertilization managements

2021 ◽  
pp. 1-11
Author(s):  
Limin Wang ◽  
Dongfeng Huang
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Soil Properties ◽  
Microbial Communities ◽  
Community Composition ◽  
Microbial Community Composition ◽  
Alpha Diversity ◽  
Phosphorus Fertilization ◽  
Bacterial Phyla ◽  
P Fertilizer

Microbes play vital roles in soil quality; however, their response to N (nitrogen) and P (phosphorus) fertilization in acidic paddy soils of subtropical China remains poorly understood. Here, a 10-year field experiment was conducted to evaluate the effects of different fertilization treatments on microbial communities by Illumina MiSeq sequencing. The results showed that different fertilization treatments did not exert a significant effect on microbial alpha diversity, but altered soil properties, and thus affected microbial community composition. The microbial communities in the T1 (optimized N and P fertilizer) and T2 (excessive N fertilizer) treated soils differed from those in the T0 (no N and P fertilizer) and T3 (excessive P fertilizer) treated soils. In addition, the bacterial phyla Proteobacteria, Chloroflexi, and Acidobacteria, and the fungal phyla Ascomycota and Basidiomycota dominated all the fertilized treatments. Soil total potassium (TK) concentration was the most important factor driving the variation in bacterial community structure under different fertilization regimes, while the major factors shaping fungal community structure were soil TN and NO3–-N (nitrate N). These findings indicate that optimization of N and P application rates might result in variations in soil properties, which changed the microbial community structure in the present study.

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