scholarly journals Molecular and morphological congruence of three new crypticNeopetrosiaspp. in the Caribbean

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6371 ◽  
Author(s):  
Jan Vicente ◽  
Jaime Andrés Ríos ◽  
Sven Zea ◽  
Robert J. Toonen
Keyword(s):  
Dna Sequences ◽  
Morphological Characters ◽  
28S Rrna ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Remote Island ◽  
The Caribbean ◽  
Morphological Differences ◽  
Definition Of ◽  
Mitochondrial Cytochrome Oxidase

Neopetrosia proxima(Porifera: Demospongiae: Haplosclerida) is described as a morphologically variable sponge common on shallow reefs of the Caribbean. However, the range of morphological and reproductive variation within putativeN. proximaled us to hypothesize that such variability may be indicative of cryptic species rather than plasticity. Using DNA sequences and morphological characters we confirmed the presence of three previously undescribed species ofNeopetrosia. Morphological differences of each new congener were best resolved by partial gene sequences of the mitochondrial cytochrome oxidase subunit 1 over nuclear ones (18S rRNA and 28S rRNA). Several new characters forNeopetrosiawere revealed by each new species. For example,N. dendrocrevaceasp. nov. andN. cristatasp. nov. showed the presence of grooves on the surface of the sponge body that converge at the oscula, and a more disorganized skeleton than previously defined for the genus.N. sigmaferasp. nov. adds the (1) presence of sigma microscleres, (2) significantly wider/longer oxeas (>200 μm), and (3) the presence of parenchymella larvae. Sampling of conspecifics throughout several locations in the Caribbean revealed larger spicules in habitats closer to the continental shelf than those in remote island locations. Our study highlights the importance of integrating molecular and morphological systematics for the discrimination of newNeopetrosiaspp. despite belonging to one of several polyphyletic groups (families, genera) within the current definition of the order Haplosclerida.

Aphelenchoidid nematodes associated with two dominant Ficus species in Aceh, Indonesia

Nematology ◽  
2017 ◽  
Vol 19 (3) ◽  
pp. 323-331 ◽  
Author(s):  
Rina Sriwati ◽  
Yuko Takeuchi-Kaneko ◽  
J. Jauharlina ◽  
Natsumi Kanzaki
Keyword(s):  
Ribosomal Rna ◽  
Dna Sequences ◽  
Large Subunit ◽  
Type B ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Molecular Sequences ◽  
Indonesian Population ◽  
Type C ◽  
Mitochondrial Cytochrome Oxidase

Aphelenchoidid nematodes associated with the syconia of two dominant fig species,Ficus hispidaandF. racemosa, were surveyed in Banda Aceh, Indonesia. Nematodes were isolated from sycones and pollinating wasps of these two fig species from four localities in the area, and identified based on the molecular sequences of two genetic loci, D2-D3 expansion segments of large subunit ribosomal RNA (D2-D3 LSU) and mitochondrial cytochrome oxidase subunit I (mtCOI). Molecular sequences of D2-D3 LSU and mtCOI were successfully determined for 44 and 19 individual nematodes, respectively, and these sequences were separated into four clades,i.e., types A-D of D2-D3 LSU and types I-IV of mtCOI. Phylogenetic analysis of the DNA sequences deposited in the GenBank database showed that the DNA sequences corresponded to three species, namely,Martininema baculum(type B/II),Ficophagus fleckeri(types A/I, D/IV) andF. cf.centerae(type C/III). Within these species,F. fleckeriwas separated into two clades as suggested in previous studies and thus it may possibly reflect the existence of two different taxa,F. fleckeriand a cryptic species. The IndonesianF. cf.centeraewas monophyletic with, but clearly separated from, the Chinese population ofF. centeraeand thus the Indonesian population is potentially an undescribed species. Overall, the species composition of fig-associated aphelenchoidids in the Aceh region seemed intermediate between continental Chinese and Australian species. However, further material collections followed by detailed morphological analyses are necessary to characterise or describe these fig-associated aphelenchoidids in Indonesia.

Taxonomy of the Oulimnius tuberculatus species group (Coleoptera: Elmidae) based on molecular and morphological data

Zootaxa ◽  
2010 ◽  
Vol 2670 (1) ◽  
pp. 59 ◽  
Author(s):  
FEDOR ČIAMPOR JR. ◽  
JÁN KODADA
Keyword(s):  
Taxonomic Status ◽  
Distinct Species ◽  
Morphological Characters ◽  
Molecular Data ◽  
Species Group ◽  
Morphological Data ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
16S Rna ◽  
Mitochondrial Cytochrome Oxidase

Two mitochondrial (cytochrome oxidase subunit I, 16S RNA) and one nuclear (18S RNA) gene fragments were used to analyse relationships within the Oulimnius tuberculatus species group. The results of molecular data analysis are compared with morphological characters, and the distributional patterns and taxonomic status of recognized taxa are addressed. Based on molecular data, Oulimnius perezi is re-validated as a distinct species, and the discovery of a possible new species from northwest Italy is reported. The possible origins and times of speciation within the species group are discussed.

scholarly journals MtCOI DNA sequences from Sycanus aurantiacus (Hemiptera: Heteroptera: Reduviidae) provide evidence of a possible new harpactorine species from Bali, Indonesia

2021 ◽  
Vol 18 (1) ◽  
pp. 74
Author(s):  
I Putu Sudiarta ◽  
Dewa Gede Wiryangga Selangga ◽  
Gusti Ngurah Alit Susanta Wirya ◽  
Ketut Ayu Yuliadhi ◽  
I Wayan Susila ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Sequences ◽  
Genetic Similarity ◽  
Mitochondrial Cytochrome ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
The Usa ◽  
Close Genetic Relationship ◽  
Mitochondrial Cytochrome Oxidase ◽  
Mtcoi Gene

Sycanus aurantiacus Ishikawa & Okajima, found in Bali, was first described in 2007 as a new harpactorine species based on morphological and biological characteristics; however, its genome has not yet been sequenced. In this study, we examine the mitochondrial cytochrome oxidase subunit I (MtCOI) nucleotide sequence of S. aurantiacus in order to determine whether it represents a new harpactorine species. A sample from Pancasari, Bali, Indonesia was collected at the same location S. aurantiacus was first discovered in 2007. The selected mtCOI gene (650 bp) was successfully amplified using mtCOI primer pairs LCO1490 and HCO2198, and the resulting MtCOI sequence of the S. aurantiacus sample was compared with those from other hapactorine species recorded in GenBank. This comparison revealed low genetic similarity between S. aurantiacus and most other harpactorine species worldwide, except for the Genus Sycanus (JQ888697) from USA whose mtCOI shares approximately 91% similarity with the Pancasari sample. Phylogenetic analysis indicated a close genetic relationship between Sycanus from Bali and the Genus Sycanus (JQ888697) from the USA. The mtCOI sequence of S. aurantiacus had not been recorded previously, and our comparison with existing Sycanus sequences provides support to the understanding that S. aurantiacus is indeed its own species.

Deep into darkness: the first stygobitic species of freshwater shrimp of the genus Caridina (Crustacea: Decapoda: Atyidae) from Northern Vietnam

Zootaxa ◽  
2021 ◽  
Vol 4933 (3) ◽  
pp. 422-434
Author(s):  
DO VAN TU ◽  
CAO THI KIM THU ◽  
THOMAS VON RINTELEN
Keyword(s):  
Ribosomal Rna ◽  
Morphological Characters ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Northern Vietnam ◽  
Freshwater Shrimps ◽  
Molecular Phylogenetic ◽  
Rna Genes ◽  
Mitochondrial Cytochrome Oxidase ◽  
Molecular Phylogenetic Data

The first stygobitic species of atyid freshwater shrimps from Vietnam is described from Cuc Phuong National Park, Northern Vietnam. Caridina thachlam sp. nov. can be distinguished from its congeners by a suite of morphological characters such as strongly reduced eyes without eyestalk, short and almost unarmed rostrum and slender pereiopods. Molecular phylogenetic data from the mitochondrial cytochrome oxidase subunit I (COI) and 16S ribosomal RNA genes also support the distinctiveness of the new species from all other species that we have examined. 

scholarly journals Lysiphlebus orientalis (Hymenoptera, Braconidae), a new invasive aphid parasitoid in Europe – evidence from molecular markers

2013 ◽  
Vol 103 (4) ◽  
pp. 451-457 ◽  
Author(s):  
Andjeljko Petrović ◽  
Milana Mitrović ◽  
Petr Starý ◽  
Olivera Petrović-Obradović ◽  
Vladimir Žikić ◽  
...  
Keyword(s):  
Host Range ◽  
Far East ◽  
Field Studies ◽  
Morphological Characters ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Aphid Parasitoid ◽  
The Past ◽  
Far Eastern ◽  
Mitochondrial Cytochrome Oxidase

AbstractWe report the occurrence of Lysiphlebus orientalis in Serbia, an aphid parasitoid from the Far East that is new to Europe and has the potential to become invasive. Our finding based on morphological characters is confirmed by analyses of mitochondrial cytochrome oxidase subunit I sequences. An increase in number and an expansion of the host range were observed during field studies over the past two years, and it is determined that the current host range encompasses nine aphid hosts on 12 different host plants, forming 13 tri-trophic associations. A host range determined for European populations of L. orientalis appears wider compared with that in its Far Eastern native habitats where Aphis glycines Mats. is the sole known host. Moreover, it overlaps considerably with the host ranges of European parasitoids that play an important role in the natural control of pest aphids.

First rearing record and DNA sequence of Chromatomyia aizoon (Hering, 1932) (Diptera: Agromyzidae) associated with Saxifraga paniculata Miller from the Czech Republic

2020 ◽  
Vol 69 (3) ◽  
pp. 283-288
Author(s):  
Miloš Černý ◽  
Jiří Kocián ◽  
Jan Ševčík
Keyword(s):  
Czech Republic ◽  
Cytochrome Oxidase ◽  
Dna Sequence ◽  
Partial Sequence ◽  
Mitochondrial Cytochrome ◽  
Cytochrome Oxidase Subunit ◽  
Cytochrome Oxidase Subunit I ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
The Czech Republic ◽  
Mitochondrial Cytochrome Oxidase

Abstract A male of Chromatomyia aizoon (Hering, 1932) (Diptera: Agromyzidae) was reared from a leaf mine at Saxifraga paniculata collected in Štramberk (Northern Moravia, Czech Republic). Partial sequence of the mitochondrial cytochrome oxidase subunit I (COI barcode region, 658 bp) is also provided.

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