scholarly journals Isolation and identification of marine strains of Stenotrophomona maltophilia with high chitinolytic activity

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6102 ◽  
Author(s):  
Roger Salas-Ovilla ◽  
Didiana Gálvez-López ◽  
Alfredo Vázquez-Ovando ◽  
Miguel Salvador-Figueroa ◽  
Raymundo Rosas-Quijano
Keyword(s):  
Organic Compound ◽  
Bacterial Communities ◽  
Stenotrophomonas Maltophilia ◽  
Morphological Traits ◽  
Specific Activity ◽  
Agar Medium ◽  
Chitinolytic Activity ◽  
Isolation And Identification ◽  
Carbon And Nitrogen ◽  
Dilution Assay

Chitin is the second most abundant organic compound in nature and represents a rich carbon and nitrogen source that is primarily transformed by bacterial communities. Bacteria capable of gradually hydrolyzing chitin into N-acetylglucosamine monomers can have applications in the transformation of residues from shrimp and other crustaceans. The objective of the present study was to isolate, characterize and identify microorganisms with high chitinolytic activity. These microorganisms were isolated and characterized based on macro- and microscopic morphological traits. Strains were selected on colloidal chitin agar medium primarily based on a hydrolysis halo larger than 2 mm and a growing phase no longer than 6 days. Secondary selection consisted of semi-quantitative evaluation of chitinolytic activity with a drop dilution assay. From the above, ten strains were selected. Then, strain-specific activity was evaluated. The B4 strain showed the highest specific activity, which was 6,677.07 U/mg protein. Molecular identification indicated that the isolated strains belong to the species Stenotrophomonas maltophilia.

THE PREDOMINANT BACTERIA IN NATURAL ZOOGLOEAL COLONIES: I. ISOLATION AND IDENTIFICATION

1967 ◽  
Vol 13 (12) ◽  
pp. 1671-1682 ◽  
Author(s):  
Richard F. Unz ◽  
Norman C. Dondero
Keyword(s):  
Waste Water ◽  
Single Cell ◽  
Agar Medium ◽  
Isolation And Identification ◽  
Group I ◽  
Yeast Autolysate ◽  
Conventional Methods ◽  
Group Ii ◽  
Produce Acid

Direct, single-cell isolations of bacteria, primarily from natural, branching, waste water zoogloeas, were made by micromanipulation. Isolations were also made by conventional methods. Direct isolates were classified, chiefly with regard to zoogloea formation, into two groups designated group I (zoogloea-forming) and group II (nonzoogloea-forming). Casitone – glycerol – yeast autolysate agar medium was best for the isolation of group I bacteria. Group I isolates reduced nitrate to gas, possessed urease and catalase, and gave positive oxidase reactions. They were generally able to hydrolyze gelatin but, with one exception, did not produce acid from carbohydrates, and none produced H2S, indole, or acetylmethylcarbinol or utilized Koser citrate. Group II strains were usually more diverse on differential tests and could be distinguished from group I strains. Group I strains were characterized as Zoogloea strains and were found to be the predominant bacteria in natural, branching, zoogloeal colonies.

scholarly journals EXPRESSION OF b-XYLOSIDASE ENCODING GENE IN PHIS/ Bacillus megaterium MS SYSTEM

2015 ◽  
Vol 2 (1) ◽  
pp. 25 ◽  
Author(s):  
Sri Sumarsih
Keyword(s):  
Culture Medium ◽  
Culture Supernatant ◽  
Specific Activity ◽  
Agar Medium ◽  
Nitrilotriacetic Acid ◽  
Protoplast Transformation ◽  
E Coli ◽  
Recombinant Cells ◽  
Relative Molecular Weight ◽  
Encoding Gene

b-Xylosidase encoding gene from G. thermoleovorans IT-08 had been expressed in the pHIS1525/ B. megaterium MS941 system. The b-xylosidase gene (xyl) was inserted into plasmid pHIS1525 and propagated in E. coli DH10b. The recombinant plasmid was transformed into B. megaterium MS941 by protoplast transformation. Transformants were selected by growing the recombinant cells on solid LB medium containing tetracycline (10 µg/ ml). The expression of the b-xylosidase gene was assayed by overlaid the recombinant B. megaterium MS941 cell with agar medium containing 0.2% ethylumbelliferyl-b-D-xyloside (MUX). This research showed that the b-xylosidase gene was succesfully sub-cloned in pHIS1525 system and expressed by the recombinant B. megaterium MS941. Theaddition of 0.5% xylose into the culture medium could increase the activity of recombinantactivity of recombinant of recombinantb-xylosidase by 2.74 fold. The recombinant B. megaterium MS941 secreted 75.56% of the expressed b-xylosidase into culture medium. The crude extract b-xylosidase showed the optimum activity at 50° C and pH 6. The recombinant b-xylosidase was purified from culture supernatant by affinity chromatographic method using agarose containing Ni-NTA (Nickel-Nitrilotriacetic acid). The pure b-xylosidase showed a specific activity of 10.06 Unit/mg protein and relative molecular weight ± 58 kDa.

scholarly journals Novel Fibrinolytic Protease Producing Streptomyces radiopugnans VITSD8 from Marine Sponges

Marine Drugs ◽  
2019 ◽  
Vol 17 (3) ◽  
pp. 164 ◽  
Author(s):  
Dhamodharan D ◽  
Jemimah S ◽  
Merlyn S ◽  
Subathra C
Keyword(s):  
Tamil Nadu ◽  
Specific Activity ◽  
Blood Clot ◽  
Exchange Chromatography ◽  
Marine Sponges ◽  
Protease Production ◽  
Clot Lysis ◽  
Carbon And Nitrogen ◽  
Lysis Activity

Fibrinolytic enzymes have received more attention due to their medicinal potential for thrombolytic diseases. The aim of this study is to characterize the in vitro fibrinolytic nature of purified protease producing Streptomyces radiopugnans VITSD8 from marine brown tube sponges Agelas conifera. Three varieties of sponge were collected from the Rameshwaram Sea coast, Tamil Nadu, India. The fibrinolytic activity of Streptomyces sp. was screened and determined by casein plasminogen plate and fibrin plate methods respectively. The crude caseinolytic protease was purified using ammonium sulfate fractionation, affinity and ion-exchange chromatography. Based on the morphological, biochemical, and molecular characterization, the isolate VITSD8 was confirmed as Streptomyces radiopugnans. Maltose and peptone were found to be the best carbon and nitrogen sources for the production of fibrinolytic protease. The carbon and nitrogen source peptone showed (781 U/mL) enzyme activity. The optimum pH and temperature for fibrinolytic protease production was found to be 7.0 and 33 °C respectively. The purified enzyme showed a maximum specific activity of 3891 U. The blood clot lysis activity was compared with the standard, and it was concluded that a minimum of 0.18 U (10 µL) of purified protease was required to dissolve the blood clot. This is the first report which exploits the fibrinolytic protease activity of Streptomyces radiopugnans VITSD8 extracted from a marine sponge. Hence the investigation suggests a potential benefit of purified fibrinolytic protease which will serve as an excellent clot buster alternative.

Phage therapy of corrosion-producing bacterium Stenotrophomonas maltophilia using isolated lytic bacteriophages

2017 ◽  
Vol 64 (6) ◽  
pp. 607-612 ◽  
Author(s):  
Arezoo Pedramfar ◽  
Keivan Beheshti Maal ◽  
Sayed Hossein Mirdamadian
Keyword(s):  
Stenotrophomonas Maltophilia ◽  
Culture Medium ◽  
Phage Therapy ◽  
Isolation And Identification ◽  
Content Type ◽  
Sulfate Reducing ◽  
Transmission Electron ◽  
Reducing Bacteria ◽  
Pipeline Corrosion ◽  
Selective Culture Medium

Purpose Corrosion-producing microorganisms have different physiology and include sulfate-reducing bacteria, iron oxidizers and magnesium oxidizers. Biocorrosion has been seen in various industries, especially the petrochemicals and oil industries. One proposal to solve this problem is the use of bacteriophages to treat the bacteria-caused corrosion. The aims of this study were isolation and identification of corrosion-producing bacteria from petroleum pipeline corrosion as well as finding their specific bacteriophages for phage therapy purposes. Design/methodology/approach The sample pipes with the corrosion were obtained from the Gandomkar petroleum pipeline station, Chaharmahal and Bakhtiari, Iran. For screening the corrosion-producing bacteria, the rusted pipe samples were cultured in a selective culture medium, manganese agar. The purified individual colonies were subjected to molecular examinations. For isolating bacteriophages from silversmithing workshops wastewater in Isfahan, whole plate titration methods and transmission electron microscopy were used to isolate and detect phages. Findings The cultivation of corrosion-based material on manganese agar after 18 hours incubation at 30°C resulted in the isolation of cream-colored colonies. The microscopic examinations showed Gram-negative coccobacilli. Based on molecular examinations, the isolated bacteria were identified as Stenotrophomonas maltophilia strain PBM-IAUF-2 with Genebank accession number of KU145278.1. The found bacteriophage was related to the Siphoviridae family of phages. Originality/value This paper is the first report of isolation and identification of corrosion-producing bacteria and its specific lytic phages from Gandomkar petroleum pipeline station, Chaharmahal and Bakhtiari, Iran. The biological procedures for preventing the microbial corrosion could be an asset and considered as a potential in the petroleum and industrial microbiology. Phage therapy is considered as one of the economical methods for reducing the biocorrosion.

scholarly journals Screening of Keratinolytic Bacteria from Poultry Wastes

1970 ◽  
Vol 45 (3) ◽  
pp. 261-266 ◽  
Author(s):  
Z Jahan ◽  
SN Khan ◽  
M Mozammel Hoque
Keyword(s):  
Large Scale ◽  
Biochemical Analysis ◽  
Specific Activity ◽  
Basal Medium ◽  
Bacillus Sp ◽  
Carbon And Nitrogen ◽  
Feather Waste ◽  
Key Words Identification ◽  
Bacillus Spp ◽  
Poultry Feather

The aim of this study was to characterize keratinolytic bacteria isolated from feather waste. Six isolates were recovered from poultry feather- decomposed materials. Isolate Z3 and Z4 showed important feather degrading activity when grown on basal medium containing 1% native feather as the only source of energy, carbon and nitrogen. All isolates were Gram positive and rod-shaped bacilli. Based on microscopic and biochemical analysis, the isolates were identified as Bacillus spp. Keratinolytic activities of these isolates were measured after cultivation of the bacteria on raw feathers. Maximum keratinase activity was showed by the isolate Z4 (22.3 U/ml) with the specific activity of 40.5 U/mg. Bacillus sp. Z4 is a potent producer of keratinase, which can be used for production of the enzyme in large scale. Key words: Identification; Purification; Characterization; Keratinase; Feather DOI: 10.3329/bjsir.v45i3.6535Bangladesh J. Sci. Ind. Res. 45(3), 261-266, 2010

scholarly journals Evaluation of a new chromogenic agar medium for isolation and identification of Group B streptococci

2006 ◽  
Vol 43 (6) ◽  
pp. 615-618 ◽  
Author(s):  
J.D. Perry ◽  
M. Oliver ◽  
A. Nicholson ◽  
J. Wright ◽  
F.K. Gould
Keyword(s):  
Agar Medium ◽  
Group B Streptococci ◽  
Isolation And Identification ◽  
Chromogenic Agar ◽  
Group B

scholarly journals Isolasi dan Identifikasi Bakteri Kitinolitik yang terdapat pada Cangkang Lobster Air Tawar (Cherax Quadricarinatus) [Isolation and Identification of Chytinolitic Bacteria from The Crayfish (Cherax Quadricarinatus) Shell]

2019 ◽  
Vol 8 (1) ◽  
pp. 16
Author(s):  
Hari Suprapto ◽  
Sudarno Sudarno ◽  
Istikhara Mentari Tito
Keyword(s):  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Test Medium ◽  
Clear Zone ◽  
Chitinolytic Activity ◽  
Isolation And Identification ◽  
Cherax Quadricarinatus ◽  
Chitinolytic Bacteria ◽  
Bacterium Species ◽  
Observation Method

AbstrakIndonesia memiliki potensi perikanan yang sangat tinggi, salah satunya adalah lobster. Ekspor lobster air tawar cenderung meningkat tiap tahun. Total ekspor hasil lobster budidaya mencapai 94.511 ton/tahun. Pangsa pasar lobster air tawar tidak hanya terbatas di dalam negeri saja tetapi juga ke luar negeri. Penelitian ini bertujuan untuk mengetahui adanya bakteri kitinolitik dan juga jenis-jenis bakteri kitinolitik yang terdapat pada cangkang lobster air tawar. Penelitian ini menggunakan Penelitian ini menggunakan metode observasi untuk mengetahui adanya bakteri kitinolitik yang ada pada cangkang lobster air tawar. Data yang digunakan dalam penelitian ini adalah secara deskriptif. Data yang diperoleh dianalisis secara deskriptif yaitu penyajian data tentang morfologi dan karakteristik dari bakteri kitinolitik yang diisolasi dari cangkang lobser air tawar (Cherax quadricarinatus) dan dibandingkan dengan morfologi dan karakteristik bakteri kitinolitik dengan literatur yang sesuai. Hasil penelitian ini adalah diperoleh bahwa bakteri yang diisolasi dari cangkang lobster air tawar (Cherax quadricarinatus) dapat tumbuh dan berkembang pada media uji. Kemudian hasil uji hidrolisis bakteri kitinolitik ditandai dengan adanya zona bening yang dihasilkan dari bakteri tersebut. Dari hasil pengujian tersebut didapatkan jenis – jenis bakteri yaitu Aeromonas sp., Bacillus sp. dan Pseudomonas sp. AbstractIndonesia have highest potential of fishery. One of them is Lobster. It has increasing slightly, approximately 94.511 ton/years. The aims this research to determine the presence of chitinolytic bacteria and identify its species in crayfish shells. This research was performed via observation method to determine the presence of chitinolytic bacteria, which are exist in crayfish (cherax quadricarinatus) shell. The data used in this research is descriptive. The data descriptively analyzed represented the morphology and characteristics of chitinolytic bacteria isolated from crayfish (Cherax quadricarinatus) and comparison the morphology and characteristics of chitinolytic bacteria with appropriate literature. These results obtained that the bacteria isolated from crayfish (Cherax quadricarinatus) shell can grow and develop in the test medium. Then, chitinolytic activity was signed by the formation of clear zone on the test medium. The results obtained several bacterium species including Aeromonas sp., Bacillus sp. and Pseudomonas sp. 

scholarly journals Optimization of alkali-thermostable and cellulase-free eylanase production from Bacillus Sp.

2012 ◽  
Vol 19 ◽  
pp. 7-14
Author(s):  
SCD Sharma ◽  
MS Shovon ◽  
AKM Asaduzzaman ◽  
MG Sarowar Jahan ◽  
T Yeasmin ◽  
...  
Keyword(s):  
Wheat Bran ◽  
Agar Medium ◽  
Xylanase Activity ◽  
Effect Of Temperature ◽  
Bacillus Sp ◽  
Dilution Technique ◽  
Isolation And Identification ◽  
Xylanase Production ◽  
Optimization Of Process Parameters ◽  
Bacterium Bacillus

Context: To analyze the nutritional and physicochemical parameters for the production of alkali-thermostable and cellulase free xylanase from bacteria. Objectives: The aim of this study was to isolation and identification and of alkali-thermostable and cellulase free xylanase producing bacteria from soil as well as optimization of process parameters for xylanase production. Materials and Methods: The bacterium Bacillus sp. was isolated from soil by serial dilution technique on xylan agar medium and identified by morphological and biochemical studies. The production of xylanase was carried out on xylan broth medium and xylanase activity was assayed by dinitrosalicylic acid (DNS) method. The effect of cultural parameters on the production of xylanase was determined by measuring the activity of xylanase. The effect of temperature and pH on the activity of partially purified xylanase as well as substrate specificity of xylanase were examined. Results: The maximum xylanase production (4000 U/L) by a Bacillus sp. was attained when the medium containing 0.5% wheat bran xylan and peptone at pH 8.0 and 50-55°C within 48-60 h. The partially purified xylanase was optimally active at pH 9.0 and 55°C. The xylanase showed high substrate activity towards wheat bran xylan but no activity towards cellulose, carboxymethyl cellulose and starch. Thus the enzyme was alkali-thermostable and cellulase free xylanase. Conclusion: The results obtained in this study suggest that the Bacillus sp. used is highly potential and useful for the production of cellulase free xylanase. DOI: http://dx.doi.org/10.3329/jbs.v19i0.12994 J. bio-sci. 19: 7-14, 2011

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