scholarly journals Exploring nutrient limitation for polyhydroxyalkanoates synthesis by newly isolated strains ofAeromonassp. using biodiesel-derived glycerol as a substrate

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5838 ◽  
Author(s):  
Justyna Możejko-Ciesielska ◽  
Tomasz Pokoj
Keyword(s):  
Crude Glycerol ◽  
Municipal Wastewater ◽  
Nitrogen Deficiency ◽  
Treatment Plant ◽  
Second Step ◽  
Rrna Gene ◽  
Municipal Wastewater Treatment ◽  
Nitrogen And Phosphorus ◽  
Pure Glycerol ◽  
Biodiesel Industry

Aeromonasspp. strains isolated from activated sludge in a municipal wastewater treatment plant were found to be able to synthesize polyhydroxyalkanoates (PHA) utilizing pure and crude glycerol. The 16S rRNA gene sequence of the isolates exhibited similarity toAeromonas hydrophila,A. aquatica, andA. salmonicida. Our results confirmed that the adequate supply of nitrogen and phosphorus during culture in 250-ml shake flasks did not stimulate the synthesis of PHAs. The results indicate that the PHA content of cells was higher under a phosphorus-limiting environment compared to nitrogen starvation. In the two-stage cultivation using glucose (in the first step) and crude glycerol from biodiesel industry (in the second step) as a component of the growth medium, the analyzed strains grew to 3.06 g/l of cell dry weight containing up to 22% of PHAs. Furthermore, during the same culture strategy up to 42% of PHAs were extracted, when in the second step of the process,Aeromonassp. AC_03 was grown on pure glycerol under phosphorus limitation. The purified biopolymer was confirmed to be polyhydroxybutyrate.Aeromonassp. AC_02 was also capable to accumulate the poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer when pure glycerol was added as a substrate under nitrogen-deficiency one-step bioprocess. Our results confirm that due to the biopolymer productivity, newly isolated strains could be exploited for obtaining valuable biopolymers using wastes generated from biodiesel industry.

Upgrading of a Combined Industrial and Municipal Wastewater Treatment Plant – The Fredericia Plant, Denmark

1990 ◽  
Vol 22 (7-8) ◽  
pp. 225-232
Author(s):  
G. Petersen
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Treatment Strategies ◽  
Pilot Scale ◽  
Municipal Wastewater Treatment ◽  
Nitrogen And Phosphorus ◽  
Effluent Quality ◽  
Biological Nitrogen

The upgrading of an existing mechanical wastewater treatment plant to meet the new standards for effluent quality in the Municipality of Fredericia, Denmark, are presented. The Municipality has a lot of very big organic and inorganic industries, which leaves several different possibilities for treatment strategies. In 1987 pilot-scale tests were carried out to study the effects of various combinations of wastewater types on the treatment efficiency, and the tests resulted in two main solutions for the wastewater treatment system. The pilot-scale tests were run in a two - stage biology plant. The first stage was either a BIOSORPTION unit or a PRE-DENITRIFICATION unit. The second stage was a biological nitrogen and phosphorus removal unit (a BIO-DENIPHO unit).

Experiment Research on Performance of Step-Feed SBR Nitrogen and Phosphorus Removal

2014 ◽  
Vol 1073-1076 ◽  
pp. 849-853
Author(s):  
Xiu Bin Lv ◽  
Zhi Hong Yang ◽  
Hai Zhao Zhao ◽  
Hong Ping Chen
Keyword(s):  
Municipal Wastewater ◽  
Field Experiments ◽  
Batch Reactor ◽  
Waste Water Treatment ◽  
Treatment Plant ◽  
Water Treatment Plant ◽  
Waste Water Treatment Plant ◽  
Municipal Wastewater Treatment ◽  
Nitrogen And Phosphorus ◽  
Class A

A waste water treatment plant (WWTP) adopts sequencing batch reactor (SBR) process, which exist the problem of instable treatment effect on denitrification and dephosphorization. The total nitrogen (TN) and total phosphorus (TP) of the effluent could not reach the class A standard of discharge standard of pollutants for municipal wastewater treatment plant (GB18918-2002) (hereinafter referred to as the class A standard) as a result of different order in utilizing the carbon source between nitrification and denitrification. The step-feed procedure is used to improve the efficiency of denitrification and dephosphorization. Field experiments about the effects of the different influent distribution ratio (marked as λ) on denitrification and dephosphorization were carried out and the results showed that the effect of the effluent TN is the best and other indexes could also achieve class A standard when λ is 5:3.

scholarly journals Molecular Evidence for Novel Planctomycete Diversity in a Municipal Wastewater Treatment Plant

2003 ◽  
Vol 69 (12) ◽  
pp. 7354-7363 ◽  
Author(s):  
Rakia Chouari ◽  
Denis Le Paslier ◽  
Patrick Daegelen ◽  
Philippe Ginestet ◽  
Jean Weissenbach ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Wastewater Treatment Plant ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Rrna Gene ◽  
Municipal Wastewater Treatment ◽  
Monophyletic Cluster ◽  
Municipal Wastewater Treatment Plant

ABSTRACT We examined anoxic and aerobic basins and an anaerobic digestor of a municipal wastewater treatment plant for the presence of novel planctomycete-like diversity. Three 16S rRNA gene libraries were constructed by using a 16S rRNA-targeted universal reverse primer and a forward PCR primer specific for Planctomycetes. Phylogenetic analysis of 234 16S rRNA gene sequences defined 110 operational taxonomic units. The majority of these sequences clustered with the four known genera, Pirellula (32%), Planctomyces (18.4%), Gemmata (3.8%), and Isosphaera (0.4%). More interestingly, 42.3% of the sequences appeared to define two distantly separated monophyletic groups. The first group, represented by 35.5% of the sequences, was related to the Planctomyces group and branched as a monophyletic cluster. It exhibited between 11.9 and 20.3% 16S rRNA gene sequence dissimilarity in comparisons with cultivated planctomycetes. The second group, represented by 6.8% of the sequences, was deeply rooted within the Planctomycetales tree. It was distantly related to the anammox sequences (level of dissimilarity, 20.3 to 24.4%) and was a monophyletic cluster. The retrieved sequences extended the intralineage phylogenetic depth of the Plantomycetales from 23 to 30.6%. The lineages described here may have a broad diversity of undiscovered biochemical and metabolic novelty. We developed a new 16S rRNA-targeted oligonucleotide probe and localized members of one of the phylogenetic groups using the fluorescent in situ hybridization technique. Our results indicate that activated sludge contains very diverse representatives of this group, which grow under aerobic and anoxic conditions and even under anaerobic conditions. The majority of species in this group remain poorly characterized.

scholarly journals Implementing Solutions for Muskrat Lake

2016 ◽  
Vol 2 (1) ◽  
pp. 7-11
Author(s):  
Wilson Ho
Keyword(s):  
Biological Treatment ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Test Site ◽  
Agricultural Runoff ◽  
Municipal Wastewater Treatment ◽  
Wastewater Remediation ◽  
Short Term ◽  
Nitrogen And Phosphorus ◽  
Water Speed

Muskrat Lake has recently been suffering from nutrient overloading due to unknown causes. The purpose of this investigation is to determine where excess nutrients are concentrated in the Muskrat Lake watershed, to monitor their levels over a six-week period, and to test the BioCord Reactor as a novel solution. The reactor is commonly used in man-made wastewater remediation systems to increase the level of biological treatment, and decrease concentrations of nitrogen and phosphorus. Collection bottles were rinsed three times at each collection site before samples were taken and shipped to the Canadian Nuclear Laboratories and the Ministry of Environment for analysis, or brought back to the lab. Lab equipment was purchased from HACH Industries, and the HACH TNT Kit 843 procedure was slightly modified to scan for an ultra-low level of phosphorus. Overall, the results suggest that agricultural runoff, the mixed wood forests, and the municipal wastewater treatment plant do not have significant impacts on the watershed, but more analysis is necessary before any definitive conclusions can be drawn. Furthermore, the BioCord Reactor was shown to be ineffective at the test site due to low rainfall, water depth, and water speed. More sites need to be analyzed for a longer period of time in order to determine if this technology is feasible as a short-term remediation tool for eutrophication.

Simultaneous removal of nitrogen and phosphorus from wastewater by means of FeS-based autotrophic denitrification

2013 ◽  
Vol 67 (12) ◽  
pp. 2761-2767 ◽  
Author(s):  
Ruihua Li ◽  
Jianmin Niu ◽  
Xinmin Zhan ◽  
Bo Liu
Keyword(s):  
Municipal Wastewater ◽  
Removal Rate ◽  
Treatment Plant ◽  
Secondary Effluent ◽  
Autotrophic Denitrification ◽  
Municipal Wastewater Treatment ◽  
Nitrogen And Phosphorus ◽  
P Concentration ◽  
N Removal ◽  
Wide Ph Range

The efficacy of iron(II) sulfide (FeS)-based autotrophic denitrification in simultaneous nitrogen and phosphorus removal from wastewater was studied with batch experiments. It was efficient at a wide pH range of 5–9, and temperature range of 10–40 °C. The concentrations of NH4+-N, Mg2+ and HCO3− in the wastewater should be kept over 7.8, 0.24 and 30 mg L−1 for efficient nitrate (NO3−-N) reduction, respectively. The NO3−-N removal rate increased from 0 to 82 mg L−1 d−1 and then leveled off when the NO3−-N concentration increased from 0 to 415 mg L−1 and then to 700 mg L−1, respectively. The NO3−-N removal rate quickly increased, leveled off, and then sharply decreased when the PO43−-P concentration increased from 0 to 0.1 mg L−1, then to 114.0 mg L−1, and further to 683.8 mg L−1, respectively. The PO43−-P removal was over 98% when the PO43−-P concentration ranged 0–683.3 mg L−1. During treatment of the secondary effluent of a local municipal wastewater treatment plant containing NO3−-N of 14.9 mg L−1 and total phosphorus (TP) of 3.9 mg L−1, NO3−-N was reduced to 1.1 mg L−1 and TP was completely removed.

scholarly journals High functional diversity among Nitrospira populations that dominate rotating biological contactor microbial communities in a municipal wastewater treatment plant

2019 ◽  
Author(s):  
Emilie Spasov ◽  
Jackson M. Tsuji ◽  
Laura A. Hug ◽  
Andrew C. Doxey ◽  
Laura A. Sauder ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Municipal Wastewater ◽  
Ammonia Oxidation ◽  
Treatment Plant ◽  
16S Rrna Gene Sequencing ◽  
Model Systems ◽  
Rrna Gene ◽  
Ammonia Oxidizing Bacteria ◽  
Municipal Wastewater Treatment ◽  
Nitrite Oxidizers

AbstractNitrification, the oxidation of ammonia to nitrate via nitrite, is an important process in municipal wastewater treatment plants (WWTPs). Members of the Nitrospira genus that contribute to complete ammonia oxidation (comammox) have only recently been discovered and their relevance to engineered water treatment systems is poorly understood. This study investigated distributions of Nitrospira, ammonia-oxidizing archaea (AOA), and ammonia-oxidizing bacteria (AOB) in biofilm samples collected from tertiary rotating biological contactors (RBCs) of a municipal WWTP in Guelph, Ontario, Canada. Using quantitative PCR (qPCR), 16S rRNA gene sequencing, and metagenomics, our results demonstrate that Nitrospira species strongly dominate RBC biofilm samples and that comammox Nitrospira outnumber all other nitrifiers. Genome bins recovered from assembled metagenomes reveal multiple populations of comammox Nitrospira with distinct spatial and temporal distributions, including several taxa that are distinct from previously characterized Nitrospira members. Diverse functional profiles imply a high level of niche heterogeneity among comammox Nitrospira, in contrast to the sole detected AOA representative that was previously cultivated and characterized from the same RBC biofilm. Our metagenome bins also reveal two cyanase-encoding populations of comammox Nitrospira, suggesting an ability to degrade cyanate, which has not been shown previously for Nitrospira that are not strict nitrite oxidizers. This study demonstrates the importance of RBCs as model systems for continued investigation of environmental factors that control the distributions and activities of AOB, AOA, comammox Nitrospira, and other nitrite oxidizers.

Nitrogen and phosphorus removal using fluidized-carriers in a full-scale A2O biofilm system

2020 ◽  
Author(s):  
Keke Xiao
Keyword(s):  
Wastewater Treatment ◽  
Phosphorus Removal ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Full Scale ◽  
Anammox Bacteria ◽  
Municipal Wastewater Treatment ◽  
Nitrogen And Phosphorus ◽  
Class Level ◽  
Microbial Analysis

<p>A full-scale biofilm system using fluidized-carriers integrated with anaerobic-anoxic–aerobic process (treatment capacity of 3.75 × 10<sup>5</sup> m<sup>3</sup> d<sup>−1</sup>) was used for municipal wastewater treatment. The results indicated relatively higher removal efficiencies of 86% total nitrogen (TN), 97% ammonium (NH<sub>4</sub>-N) and 97% total phosphorus (TP) were achieved, with 0.32 mg L<sup>−1</sup> TP, 0.81 mg L<sup>−1</sup> NH<sub>4</sub>-N and 8.07 mg L<sup>−1</sup> TN in the effluent, which meet the Class A of Discharge standard of pollutants for municipal wastewater treatment plant (GB18918-2002) of China. The results of microbial analysis indicated that the dominant microorganisms in the suspended sludge were Proteobacteria and Bacteroidetes at phylum level and β-Proteobacter at class level. The dominant microorganism in the biofilm was Proteobacteria at phylum level, with γ-Proteobacter (17.5%), β-Proteobacter (14%) and δ-Proteobacter (13.08%) distributed at class levels. The presence of Proteobacteria and Bacteroidetes in this system may be related with the phosphorus removal. A reddish color biofilm was formed on the surface of fluidized-carriers in the anaerobic tank and showed specific anammox ability, this may be related with the dominance of 0.0278% Planctomycetaceae at family level and 0.0278% Planctomycetales at order level. Besides the denitrification effects, the possible anammox bacteria present in the anaerobic tank might have also contributed to high nitrogen removal efficiency.</p>

Study on the Sewage Treatment by SBBR-BAF Filled with Ceramic Filling

2011 ◽  
Vol 299-300 ◽  
pp. 3-6
Author(s):  
Wei Li ◽  
Wei Wei ◽  
Yuan Liu ◽  
Tian Jiao Wang
Keyword(s):  
Removal Efficiency ◽  
Municipal Wastewater ◽  
Sewage Treatment ◽  
Treatment Plant ◽  
Biological Aerated Filter ◽  
Municipal Wastewater Treatment ◽  
Nitrogen And Phosphorus ◽  
Save Energy ◽  
Aerated Filter ◽  
Aeration Volume

In the biological aerated filter, the removal efficiency of phosphorus is poor, while the efficiency of organics and nitrogen are very well. In order to solve these problems, the sequence bio-film reactor connects to traditional biological aerated filter, ceramic filler was added to the system, study the effect of bio-film culturing and biological disposal capacity .Control intermittent time of anaerobic and aerobic and aeration volume, the removal effect of COD 、nitrogen and phosphorus are good in the sewage . The results show that anaerobic and aerated time are 3h and 4h in SBBR, the removal efficiency of COD、NH4+-N、TN and TP are 90.07%、85.12%、81.74% and 83.63%, the concentration are 31.37mg/L, 5.15㎎/L, 6.60㎎/L and 1.10㎎/L separately. All of these satisfy theⅠ-B criteria specified in Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant (GB 18918-2002). The system of SBBR-BAF run stably, removal sewage perfectly, avoid return and mud, save energy consumption.

scholarly journals Flavobacterium defluvii sp. nov., isolated from activated sludge

2007 ◽  
Vol 57 (2) ◽  
pp. 233-237 ◽  
Author(s):  
Minjeong Park ◽  
Seung Hyun Ryu ◽  
Thu-Huong Thi Vu ◽  
Hyeon-Su Ro ◽  
Pil-Yong Yun ◽  
...  
Keyword(s):  
Municipal Wastewater ◽  
Sequence Similarity ◽  
Treatment Plant ◽  
Optimal Growth ◽  
Molecular Data ◽  
Gliding Motility ◽  
Rrna Gene ◽  
Municipal Wastewater Treatment ◽  
16S Rrna Gene Sequences ◽  
Flavobacterium Johnsoniae

A Gram-negative bacterium, designated strain EMB117T, was isolated from a municipal wastewater treatment plant and characterized by polyphasic taxonomy. The cells were non-spore-forming rods that showed gliding motility. Optimal growth occurred at 25–30 °C and pH 7.0–8.0. Strain EMB117T contained phosphatidylethanolamine as the predominant polar lipid, and the major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 0 3-OH and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH). The G+C content of the genomic DNA was 33.5 mol% and the major isoprenoid quinone was MK-6. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain EMB117T belonged to the genus Flavobacterium and was most closely related to Flavobacterium johnsoniae DSM 425T (97.8 % sequence similarity). The DNA–DNA relatedness between strain EMB117T and F. johnsoniae ATCC 17061T was about 18 %. On the basis of the phenotypic, chemotaxonomic and molecular data, strain EMB117T represents a novel species within the genus Flavobacterium, for which the name Flavobacterium defluvii sp. nov. is proposed. The type strain is EMB117T (=KCTC 12612T=DSM 17963T).

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