scholarly journals Starch biotransformation into isomaltooligosaccharides using thermostable alpha-glucosidase from Geobacillus stearothermophilus

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5086 ◽  
Author(s):  
Peng Chen ◽  
Ruixiang Xu ◽  
Jianhui Wang ◽  
Zhengrong Wu ◽  
Lei Yan ◽  
...  
Keyword(s):  
High Performance ◽  
Crosslinking Agent ◽  
Small Scale ◽  
Cross Linking ◽  
Geobacillus Stearothermophilus ◽  
Isolation And Purification ◽  
Chitosan Membrane ◽  
Scale Preparation ◽  
Layer Chromatography ◽  
Alpha Glucosidase

The present study first identified the biotransformation of starch as a novel preparation method was investigated using the alpha-transglucosidase-producing Geobacillus stearothermophilus U2. Subsequently, 5 L- and 20 L-scale fermentations were performed. After isolation and purification, liquid alpha-glucosidase preparations were obtained. Through covalent cross-linking and adsorption cross-linking using chitosan as the carrier and glutaraldehyde as the crosslinking agent, the conditions for immobilization of alpha-glucosidase on chitosan were determined. Moreover, Isomaltooligosaccharides (IMOs) were then prepared using chitosan membrane-immobilized alpha-glucosidase, beta-amylase, pullulanase, fungal alpha-amylase and starch as substrate. The mixed syrup that contained IMOs was evaluated and analyzed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). In addition, small-scale preparation of IMOs was performed. These results are a strong indication that the alpha-transglucosidase-producing G. stearothermophilus as a potential application technique can be successfully used to prepare industrial IMOs.

scholarly journals Identification and Quantification of an Adulterant in a Dietary Supplement Marketed for Sexual Enhancement

2018 ◽  
Vol 1 (4) ◽  
pp. 25-33 ◽  
Author(s):  
Flavia Redko ◽  
Sabrina Flor ◽  
Silvia Lucangioli ◽  
Jerónimo Ulloa ◽  
Rafael Ricco ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance ◽  
Spectroscopic Techniques ◽  
Easy Method ◽  
Isolation And Purification ◽  
Chromatography Analysis ◽  
Pharmaceutical Ingredients ◽  
Emerging Risk ◽  
The Mean ◽  
Layer Chromatography

In recent years, the consumption of dietary supplements (DS) has increased worldwide. In Argentina, approximately 14 million DS units were sold between 2015 and 2017. The adulteration of DS with active pharmaceutical ingredients or their analogues has been reported. This represents an alarming emerging risk to public health. The aim of this work was to detect the possible adulteration of a DS marketed in Argentina for the treatment of erectile dysfunction. Initially, thin layer chromatography analysis of the DS capsules content suggested the presence of a major compound. For the isolation and purification of this compound, an easy method consisted of a liquid-liquid extraction (water/CH2Cl2) followed by re-crystallisation from ethanol, is reported. Spectroscopic techniques such as mono- and bidimensional nuclear magnetic resonance, Fourier transform infrared spectroscopy and mass spectrometry allowed its identification as tadalafil. A rapid and reliable method was developed for the quantification of tadalafil in this DS by high performance liquid chromatography-mass spectrometry (HPLC-MS/MS). The mean content of tadalafil per capsule was 21.2 mg which represents a slightly higher value than that found in approved products in Argentina (5 or 20 mg per tablet). In addition, an undeclared alga was identified in the DS by microscopic techniques.

scholarly journals Improved Method for Isolation of Lycopsamine from Roots of Comfrey (Symphytum officinale)

2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700
Author(s):  
Damjan Janeš ◽  
Boštjan Kalamar ◽  
Samo Kreft
Keyword(s):  
High Performance ◽  
Pyrrolizidine Alkaloids ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Improved Method ◽  
Isolation And Purification ◽  
H Nmr ◽  
Symphytum Officinale ◽  
Layer Chromatography ◽  
Preparative Layer Chromatography

An improved method for the isolation and purification of pyrrolizidine alkaloids from comfrey ( Symphytum officinale L.) roots was developed, introducing very fast, selective and ion residue-free reduction of N-oxides followed by ion-exchange chromatography giving a non-aqueous solution of alkaloids, from which solvents can be easily removed. With this procedure the use of large volumes of organic solvents, very slow reduction of N-oxides and input of additional impurities was avoided. Lycopsamine, which proved to be the major alkaloid, was additionally purified by preparative layer chromatography (PLC) and high performance liquid chromatography (HPLC). The identity of the alkaloid was confirmed by 1H NMR spectroscopy and mass spectrometry.

scholarly journals Isolation and purification of plant secondary metabolites using column-chromatographic technique

2016 ◽  
Vol 11 (4) ◽  
pp. 844 ◽  
Author(s):  
Vivek K. Bajpai ◽  
Rajib Majumder ◽  
Jae Gyu Park
Keyword(s):  
Secondary Metabolites ◽  
High Performance ◽  
Plant Secondary Metabolites ◽  
Biologically Active ◽  
Chromatographic Technique ◽  
Isolation And Purification ◽  
Chromatographic Techniques ◽  
Visual Demonstration ◽  
Layer Chromatography ◽  
Column Chromatographic

<p>Chromatographic techniques have significant role in natural products chemistry as well as contribute dramatically in the discovery of novel and innovative compounds of pharmaceutical and biomedical importance. This study focused on step-by-step visual demonstration of fractionation and isolation of biologically active plant secondary metabolites using column-chromatographic techniques. Isolation of bioactive compounds using column-chromatographic involves: a) Preparation of sample; b) Packing of column; c) Pouring of sample into the column; d) Elution of fractions; and e) Analysis of each fractions using thin layer chromatography. However, depending on nature of research, compounds can be further purified using high performance liquid chromatography (HPLC), and nuclear magnetic resonance (NMR) spectral analyses.</p><p><strong>Video Clips</strong></p><p><a href="https://www.youtube.com/v/pr8mrBoI8xA">Part 1:</a> 3 min 45 sec</p><p><a href="https://www.youtube.com/v/rYrfClKn-og">Part 2:</a> 6 min 21 sec</p><p><a href="https://www.youtube.com/v/kffHXxuPwbo">Part 3</a>: 4 min 45 sec</p>

Identification of ß-carotene producing bacteria from terrestrial habitats of Jaipur, Rajasthan

2019 ◽  
Vol 48 (4) ◽  
pp. 329-336
Author(s):  
Ankit Chokriwal ◽  
Bjorn John Stephen ◽  
Abhijeet Singh
Keyword(s):  
High Performance ◽  
Design Methodology ◽  
Biochemical Characterization ◽  
Dpph Assay ◽  
Content Type ◽  
Isolation And Purification ◽  
Ic50 Value ◽  
Terrestrial Habitats ◽  
Layer Chromatography

Purpose Carotenoids are pigments that have significant value as colorants and antioxidants in pharmaceutical, food, textile and cosmetic industries. Owing to its high demand, this study aims to identify ß-carotene producing bacteria from different terrestrial habitats of Jaipur region. Design/methodology/approach In this paper, standard isolation and purification process was used, followed by colony morphology and biochemical characterization of ß-carotene producing bacteria. ß-carotene concentration was determined quantitatively using spectrophotometric method. Findings Out of 43 isolates, 21 isolates showed peak range between 400 and 500 nm confirming the presence of carotenoids. Only one bacteria SAN-A has capacity to produce ß-carotene confirmed by the thin layer chromatography and high performance liquid chromatography (HPLC) with a yield of 1.68 mg/l. The 2, 2-Diphenyl-2-picrylhydrazil (DPPH) assay showed an IC50 value of 4.0 mg/ml. Originality/value The present study revealed the presence of ß-carotene producing bacteria in the soil of different terrestrial habitat of Jaipur region which can be exploited as an economical source for ß-carotene production.

scholarly journals High-Performance Thin-Layer Chromatography/Mass Spectrometry for Rapid Analysis of Neutral Glycosphingolipids

2008 ◽  
Vol 91 (5) ◽  
pp. 1218-1226 ◽  
Author(s):  
Masao Miyazaki ◽  
Azusa Yonesige ◽  
Junko Matsuda ◽  
Yasuhiro Kuroda ◽  
Naoya Kojima ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Structural Information ◽  
Lipid Extraction ◽  
Quadrupole Ion Trap ◽  
Simple Method ◽  
Isolation And Purification ◽  
Layer Chromatography

Abstract Direct coupling of high-performance thin-layer chromatography (HPTLC) to matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MS) was shown to be a reliable and reproducible method to obtain structural information and fundamental properties of glycosphingolipids (GSLs).We report a protocol for the preparation of neutral GSL extracts frommouse tissues and demonstrate the applicability of HPTLC/MS to these preparations. The protocol consists of lipid extraction and ion exchange chromatography followed by a mild alkaline treatment and a reversed-phase cartridge extraction. Possible structures for each GSL are proposed based on HPTLC/MS analyses. This fast and simple method can be used to screen neutral GSL extracts obtained from tissues and cells without isolation and purification into individual GSLs.

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