scholarly journals Novel, non-symbiotic isolates ofNeorhizobiumfrom a dryland agricultural soil

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4776 ◽  
Author(s):  
Amalia Soenens ◽  
Juan Imperial
Keyword(s):  
Agricultural Soil ◽  
Draft Genome ◽  
Genomic Analysis ◽  
Symbiotic Genes ◽  
16S Rdna Sequence ◽  
Pcr Screening ◽  
Selective Enrichment ◽  
16S Rdna Sequence Analysis ◽  
Fast Growing Rhizobia ◽  
Large Contigs

Semi-selective enrichment, followed by PCR screening, resulted in the successful direct isolation of fast-growing Rhizobia from a dryland agricultural soil. Over 50% of these isolates belong to the genusNeorhizobium, as concluded from partialrpoBand near-complete 16S rDNA sequence analysis. Further genotypic and genomic analysis of five representative isolates confirmed that they form a coherent group withinNeorhizobium, closer toN. galegaethan to the remainingNeorhizobiumspecies, but clearly differentiated from the former, and constituting at least one new genomospecies withinNeorhizobium.All the isolates lackednodandnifsymbiotic genes but contained arepABCreplication/maintenance region, characteristic of rhizobial plasmids, within large contigs from their draft genome sequences. TheserepABCsequences were related, but not identical, torepABCsequences found in symbiotic plasmids fromN. galegae, suggesting that the non-symbiotic isolates have the potential to harbor symbiotic plasmids. This is the first report of non-symbiotic members ofNeorhizobiumfrom soil.

scholarly journals Novel, non-symbiotic isolates of Neorhizobium from a dryland agricultural soil

2018 ◽  
Author(s):  
Amalia Soenens ◽  
Juan Imperial
Keyword(s):  
Agricultural Soil ◽  
Draft Genome ◽  
Genomic Analysis ◽  
Symbiotic Genes ◽  
16S Rdna Sequence ◽  
Pcr Screening ◽  
Selective Enrichment ◽  
16S Rdna Sequence Analysis ◽  
Fast Growing Rhizobia ◽  
Large Contigs

Semi-selective enrichment, followed by PCR screening, resulted in the successful direct isolation of fast-growing Rhizobia from a dryland agricultural soil. Over 50% of these isolates belong to the genus Neorhizobium, as concluded from partial rpoB and near-complete 16S rDNA sequence analysis. Further genotypic and genomic analysis of five representative isolates confirmed that they form a coherent group within Neorhizobium, closer to N. galegae than to the remaining Neorhizobium species, but clearly differentiated from the former, and constituting at least one new genomospecies within Neorhizobium. All the isolates lacked nod and nif symbiotic genes but contained a repABC replication / maintenance region, characteristic of rhizobial plasmids, within large contigs from their draft genome sequences. These repABC sequences were related, but not identical, to repABC sequences found in symbiotic plasmids from N. galegae, suggesting that the non-symbiotic isolates have the potential to harbor symbiotic plasmids. This is the first report of non-symbiotic members of Neorhizobium from soil.

scholarly journals Novel, non-symbiotic isolates of Neorhizobium from a dryland agricultural soil

2018 ◽  
Author(s):  
Amalia Soenens ◽  
Juan Imperial
Keyword(s):  
Agricultural Soil ◽  
Draft Genome ◽  
Genomic Analysis ◽  
Symbiotic Genes ◽  
16S Rdna Sequence ◽  
Pcr Screening ◽  
Selective Enrichment ◽  
16S Rdna Sequence Analysis ◽  
Fast Growing Rhizobia ◽  
Large Contigs

Semi-selective enrichment, followed by PCR screening, resulted in the successful direct isolation of fast-growing Rhizobia from a dryland agricultural soil. Over 50% of these isolates belong to the genus Neorhizobium, as concluded from partial rpoB and near-complete 16S rDNA sequence analysis. Further genotypic and genomic analysis of five representative isolates confirmed that they form a coherent group within Neorhizobium, closer to N. galegae than to the remaining Neorhizobium species, but clearly differentiated from the former, and constituting at least one new genomospecies within Neorhizobium. All the isolates lacked nod and nif symbiotic genes but contained a repABC replication / maintenance region, characteristic of rhizobial plasmids, within large contigs from their draft genome sequences. These repABC sequences were related, but not identical, to repABC sequences found in symbiotic plasmids from N. galegae, suggesting that the non-symbiotic isolates have the potential to harbor symbiotic plasmids. This is the first report of non-symbiotic members of Neorhizobium from soil.

scholarly journals Characterization and Genomic Analysis of an Efficient Dibutyl Phthalate-Degrading Bacterium Microbacterium sp. USTB-Y

2021 ◽  
Author(s):  
ZhenZhen Zhao ◽  
Chao Liu ◽  
Qianqian Xu ◽  
Shahbaz Ahmad ◽  
Haiyang Zhang ◽  
...  
Keyword(s):  
Dibutyl Phthalate ◽  
Draft Genome ◽  
Genomic Analysis ◽  
Culture Conditions ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis ◽  
Degrading Bacterium ◽  
Holistic Understanding ◽  
Genes Encoding ◽  
Genetic Mechanisms

Abstract A promising bacterial strain for biodegrading dibutyl phthalate (DBP) was successfully isolated from activated sludge and characterized as a potential novel Microbacterium sp. USTB-Y based on 16S rDNA sequence analysis and whole genome average nucleotide identity (ANI). Initial DBP of 50 mg/L could be completely removed by USTB-Y both in mineral salt medium and in DBP artificially contaminated soil within 12 h at the optimal culture conditions of pH 7.5 and 30℃, which indicates that USTB-Y has a strong ability in DBP biodegradation. Phthalic acid (PA) was identified as the end-product of DBP biodegraded by USTB-Y using GC/MS. The draft genome of USTB-Y was sequenced by Illumina NovaSeq, and 29 and 188 genes encoding for putative esterase/carboxylesterase and hydrolase/alpha/beta hydrolase were annotated based on NR (non redundant protein sequence database) analysis, respectively. Gene3781 and gene3780 from strain USTB-Y showed 100% identity with dpeH and mpeH from Microbacterium sp. PAE-1. But no phthalate catabolic gene (pht) cluster was found in the genome of strain USTB-Y. This information will be valuable for obtaining a more holistic understanding on diverse genetic mechanisms of PAEs-biodegrading Microbacterium sp. strains.

Papaya shoot tip associated endophytic bacteria isolated from in vitro cultures and host–endophyte interaction in vitro and in vivo

2007 ◽  
Vol 53 (3) ◽  
pp. 380-390 ◽  
Author(s):  
Pious Thomas ◽  
Sima Kumari ◽  
Ganiga K. Swarna ◽  
T.K.S. Gowda
Keyword(s):  
Culture Medium ◽  
Carica Papaya ◽  
In Vitro Cultures ◽  
Dependent Manner ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis ◽  
Single Organism ◽  
Dose Dependent

Fourteen distinct bacterial clones were isolated from surface-sterilized shoot tips (~1 cm) of papaya (Carica papaya L. ‘Surya’) planted on Murashige and Skoog (MS)-based papaya culture medium (23/50 nos.) during the 2–4 week period following in vitro culturing. These isolates were ascribed to six Gram-negative genera, namely Pantoea ( P. ananatis ), Enterobacter ( E. cloacae ), Brevundimonas ( B. aurantiaca ), Sphingomonas , Methylobacterium ( M. rhodesianum ), and Agrobacterium ( A. tumefaciens ) or two Gram-positive genera, Microbacterium ( M. esteraromaticum ) and Bacillus ( B. benzoevorans ) based on 16S rDNA sequence analysis. Pantoea ananatis was the most frequently isolated organism (70% of the cultures) followed by B. benzoevorans (13%), while others were isolated from single stocks. Bacteria-harboring in vitro cultures often showed a single organism. Pantoea, Enterobacter, and Agrobacterium spp. grew actively on MS-based normal papaya medium, while Microbacterium, Brevundimonas, Bacillus, Sphingomonas, and Methylobacterium spp. failed to grow in the absence of host tissue. Supplying MS medium with tissue extract enhanced the growth of all the organisms in a dose-dependent manner, indicating reliance of the endophyte on its host. Inoculation of papaya seeds with the endophytes (20 h at OD550 = 0.5) led to delayed germination or slow seedling growth initially. However, the inhibition was overcome by 3 months and the seedlings inoculated with Pantoea, Microbacterium, or Sphingomonas spp. displayed significantly better root and shoot growths.

scholarly journals Erratum to: Deodorization of pig slurry and characterization of bacterial diversity using 16S rDNA sequence analysis

2014 ◽  
Vol 52 (12) ◽  
pp. 1056-1056
Author(s):  
Ok-Hwa Hwang ◽  
Sebastian Raveendar ◽  
Young-Ju Kim ◽  
Ji-Hun Kim ◽  
Tae-Hun Kim ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Bacterial Diversity ◽  
16S Rdna ◽  
Rdna Sequence ◽  
Pig Slurry ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis

scholarly journals Whole Genome Sequencing Reveals Identification of New Acinetobacter spp. (maqsudiensis) from Local Meat Samples Collected from Dhaka, Bangladesh

2018 ◽  
Author(s):  
Muhammad Maqsud Hossain ◽  
Abdus Sadique ◽  
Aura Rahman ◽  
Abdul Khaleque ◽  
Abdul Mueed Ibne Momen ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Genomic Analysis ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Acinetobacter Species ◽  
E Coli ◽  
Potential Marker ◽  
Meat Samples ◽  
Generation Technology

In this study we announce the draft genome sequence of a newly identified Acinetobacter species cross-reacting with E. coli serotype 0157:H7. The advent of Next-Generation technology has paved to way to discover new species which could otherwise be misidentified using conventional cultural and serotyping methods. The whole genome sequence of this isolate will help to identify potential marker/s of intervention and further genomic analysis might also shed light onto the virulence properties of this newly identified Acinetobacter species which has been provided the new name of Acinetobacter maqsudiensis.

scholarly journals Draft Genome Sequence of Mesosutterella multiformis JCM 32464T, a Member of the Family Sutterellaceae, Isolated from Human Feces

2019 ◽  
Vol 8 (24) ◽  
Author(s):  
Nao Ikeyama ◽  
Moriya Ohkuma ◽  
Mitsuo Sakamoto
Keyword(s):  
Genome Sequence ◽  
Genome Assembly ◽  
Draft Genome ◽  
Genomic Analysis ◽  
Draft Genome Sequence ◽  
Human Feces ◽  
The Family ◽  
Metabolic Activities

Here, we report the draft genome sequence of Mesosutterella multiformis JCM 32464T, a new member of the family Sutterellaceae that was isolated from human feces. The genome assembly comprised 2,621,983 bp, with a G+C content of 56.9%. This genomic analysis will be useful for understanding the metabolic activities of this asaccharolytic bacterium.

scholarly journals Draft Genome Sequence of Bacillus marisflavi CK-NBRI-03, Isolated from Agricultural Soil

2020 ◽  
Vol 9 (7) ◽  
Author(s):  
Mayank Gupta ◽  
Puneet Singh Chauhan ◽  
Sudhir K. Sopory ◽  
Sneh L. Singla-Pareek ◽  
Nidhi Adlakha ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Genome Assembly ◽  
Agricultural Soil ◽  
Draft Genome ◽  
Uttar Pradesh ◽  
Draft Genome Sequence ◽  
Positive Bacterium ◽  
Gram Positive ◽  
Content Type ◽  
Draft Genome Assembly

Here, we report the 4.34-Mb draft genome assembly of Bacillus marisflavi CK-NBRI-03 (or P3), a Gram-positive bacterium, with an average G+C content of 48.66%. P3 was isolated from agricultural soil from the Badaun (midwestern plain zone) region of Uttar Pradesh, India.

scholarly journals Gulosibacter molinativorax gen. nov., sp. nov., a molinate-degrading bacterium, and classification of ‘Brevibacterium helvolum’ DSM 20419 as Pseudoclavibacter helvolus gen. nov., sp. nov.

2004 ◽  
Vol 54 (3) ◽  
pp. 783-789 ◽  
Author(s):  
Célia M. Manaia ◽  
Balbina Nogales ◽  
Norbert Weiss ◽  
Olga C. Nunes
Keyword(s):  
Cell Wall ◽  
16S Rdna ◽  
Sequence Similarity ◽  
Rdna Sequence ◽  
Strictly Aerobic ◽  
16S Rdna Sequence ◽  
Cell Wall Peptidoglycan ◽  
16S Rdna Sequence Analysis ◽  
Degrading Bacterium ◽  
Phylogenetic Level

A Gram-positive, molinate-degrading bacterium, strain ON4T (=DSM 13485T=LMG 21909T), was isolated from a mixed bacterial culture able to mineralize the herbicide molinate. The strain was strictly aerobic, oxidase- and catalase-positive and non-acid-fast, with a growth temperature of 10–41 °C. It contained the major menaquinone MK-9 and a cell-wall peptidoglycan based on d-ornithine. 16S rDNA sequence analysis revealed that the strain formed a distinct line of descent in the family Microbacteriaceae, showing the highest 16S rDNA similarity (∼95 %) to members of the genus Curtobacterium and ‘Brevibacterium helvolum’ DSM 20419 (=ATCC 13715). The latter was reported to have the cell-wall peptidoglycan type B2γ and the major menaquinone MK-9, which are typical of Clavibacter, but it is clearly separated from this genus at the phylogenetic level. Based on low values of 16S rDNA sequence similarity to previously described genera and their distinctive phenotypic characteristics, it is proposed that strains ON4T and ‘B. helvolum’ DSM 20419 be classified as two novel genera and species, with the respective names Gulosibacter molinativorax gen. nov., sp. nov. and Pseudoclavibater helvolus gen. nov., sp. nov.

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