scholarly journals Screening of phospholipase A activity and its production by new actinomycete strains cultivated by solid-state fermentation

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3524 ◽  
Author(s):  
Priscila Sutto-Ortiz ◽  
María de los Angeles Camacho-Ruiz ◽  
Manuel R. Kirchmayr ◽  
Rosa María Camacho-Ruiz ◽  
Juan Carlos Mateos-Díaz ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
High Throughput Screening ◽  
Egg Yolk ◽  
Specific Substrate ◽  
Phospholipase Activity ◽  
High Accumulation ◽  
Egg Yolk Phosphatidylcholine ◽  
Potential Applications ◽  
Routine Assay

Novel microbial phospholipases A (PLAs) can be found in actinomycetes which have been poorly explored as producers of this activity. To investigate microbial PLA production, efficient methods are necessary such as high-throughput screening (HTS) assays for direct search of PLAs in microbial cultures and cultivation conditions to promote this activity. About 200 strains isolated with selected media for actinomycetes and mostly belonging toStreptomyces(73%) andMicromonospora(10%) genus were first screened on agar-plates containing the fluorophore rhodamine 6G and egg yolk phosphatidylcholine (PC) to detect strains producing phospholipase activity. Then, a colorimetric HTS assay for general PLA activity detection (cHTS-PLA) using enriched PC (≈60%) as substrate and cresol red as indicator was developed and applied; this cHTS-PLA assay was validated with known PLAs. For the first time, actinomycete strains were cultivated by solid-state fermentation (SSF) using PC as inductor and sugar-cane bagasse as support to produce high PLA activity (from 207 to 2,591 mU/g of support). Phospholipase activity of the enzymatic extracts from SSF was determined using the implemented cHTS-PLA assay and the PC hydrolysis products obtained, were analyzed by TLC showing the presence of lyso-PC. Three actinomycete strains of theStreptomycesgenus that stood out for high accumulation of lyso-PC, were selected and analyzed with the specific substrate 1,2-α-eleostearoyl-sn-glycero-3-phosphocholine (EEPC) in order to confirm the presence of PLA activity in their enzymatic extracts. Overall, the results obtained pave the way toward the HTS of PLA activity in crude microbial enzymatic extracts at a larger scale. The cHTS-PLA assay developed here can be also proposed as a routine assay for PLA activity determination during enzyme purification,directed evolution or mutagenesis approaches. In addition, the production of PLA activity by actinomycetes using SSF allow find and produce novel PLAs with potential applications in biotechnology.

scholarly journals Pool of Biological Resources for Potential Applications in Solid State Fermentation Obtained from a Forest Plantation of <i>Pinus pseudostrobus</i> Lindl, Mexico

2016 ◽  
Vol 06 (14) ◽  
pp. 1021-1039
Author(s):  
E. Rodríguez-Bustamante ◽  
E. Rodríguez-Flores ◽  
F. Rojas-García ◽  
A. S. Callejas-Iberri ◽  
L. M. Gallardo-Roldán ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Forest Plantation ◽  
Biological Resources ◽  
Potential Applications ◽  
Pinus Pseudostrobus

scholarly journals SCREENING, ISOLATION, AND PRODUCTION OF FUNGAL LACCASE FROM SAW MILL SOIL OF OSMANABAD.

2020 ◽  
pp. 1-4
Author(s):  
Sabina Shaikh ◽  
Prashant Dixit ◽  
Innus Shaikh
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Fungal Species ◽  
Laccase Production ◽  
Bromophenol Blue ◽  
Natural Habitats ◽  
Solid Media ◽  
Potential Applications ◽  
Laccase Enzyme ◽  
Pulp And Paper Industries

The rainforest and wood decaying habitats are the main sources for the several laccase producers, which include plant, bacteria, fungi and actinomycetes. Among these, several fungal species having laccase producing ability, which mainly includes wood rooting fungi that are often associated with lignin peroxidase or manganese dependent peroxidase or both. In view of this, we screened the natural habitats like saw mill soil and nearby places of Osmanabad district of Maharashtra for isolation of potential fungal species having laccase producing abilities. A total 45 fungal strains were isolated and screened for laccase production on solid media like Czapak dox agar medium containing guaiacol, tannic acid, bromophenol blue, and ABTS. Out of that 24 isolates showed positive results and among these S19 was found to be a potent laccase producing ability. The S19 fungus sp. subcultured on Czapak dox agar slant and screened for laccase enzyme production on solid state fermentation using wheat bran. In conclusion, we isolated the most potent laccase producing S19 fungus sp. from local natural habitats. A total 11.35 U/L of laccase enzyme obtained under optimized solid state fermentation. The enzyme may find potential applications in degradation of xenobiotics, decolourization of dyes, pulp and paper industries, depolymerisation of lignin, pigment degradation, and several other industrial processes.

scholarly journals Development and Optimization of Lipase-Catalyzed Synthesis of Phospholipids Containing 3,4-Dimethoxycinnamic Acid by Response Surface Methodology

Catalysts ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 588
Author(s):  
Magdalena Rychlicka ◽  
Natalia Niezgoda ◽  
Anna Gliszczyńska
Keyword(s):  
Response Surface Methodology ◽  
Food Additives ◽  
Reaction Medium ◽  
Egg Yolk ◽  
Molar Ratio ◽  
Molar Ratios ◽  
Egg Yolk Phosphatidylcholine ◽  
Potential Applications ◽  
Substrate Molar Ratio ◽  
Enzyme Load

The interesterification reaction of egg-yolk phosphatidylcholine (PC) with ethyl ester of 3,4-dimethoxycinnamic acid (E3,4DMCA) catalyzed by Novozym 435 in hexane as a reaction medium was shown to be an effective method for the synthesis of corresponding structured O-methylated phenophospholipids. The effects of substrate molar ratios, time of the reaction and enzyme load on the process of incorporation of 3,4DMCA into PC were evaluated by using the experimental factorial design of three factors and three levels. The results showed that a substrate molar ratio is a crucial variable for the maximization of the synthesis of 3,4-dimethoxycinnamoylated phospholipids. Under optimized parameters of 1/10 substrate molar ratio PC/E3,4DMCA, enzyme load 30% (w/w), hexane as a medium and incubation time of 3 days, the incorporation of aromatic acid into phospholipid fraction reached 21 mol%. The modified phosphatidylcholine (3,4DMCA-PC) and modified lysophosphatidylcholine (3,4DMCA-LPC) were obtained in isolated yields of 3.5% and 27.5% (w/w), respectively. The developed method of phosphatidylcholine interesterification is the first described in the literature dealing with 3,4DMCA and allows us to obtain new O-methylated phenophospholipids with potential applications as food additives or nutraceuticals with pro-health activity.

Characterization of a Novel Thermostable Enzyme from Thermus sp. 2.9 with Phospholipase and Acyltransferase Activities

2018 ◽  
Vol 28 (3) ◽  
pp. 99-106
Author(s):  
Laura Emilce Navas ◽  
Mónica Florin-Christensen ◽  
Graciela Beatriz Benintende ◽  
Rubén Oreste Zandomeni ◽  
Marcelo Facundo Berretta
Keyword(s):  
Egg Yolk ◽  
Thermostable Enzyme ◽  
Industrial Processes ◽  
Gene Encoding ◽  
High Thermostability ◽  
Egg Yolk Phosphatidylcholine ◽  
Wide Range ◽  
Potential Applications ◽  
Four Blocks ◽  
Differential Properties

Phospholipases are classified in different enzyme families according to the ester bond they cleave within phospholipids. The use of phospholipases in industrial processes has prompted the search for new enzymes with differential properties. A gene encoding a novel phospholipase (PLP_2.9) was identified in the genome of the thermophilic strain <i>Thermus</i> sp. 2.9. The analysis of the primary sequence unveiled a patatin-like domain. The alignment of the amino acid sequence of PLP_2.9 to other bacterial patatin-related proteins showed that the four blocks characteristic of this type of phospholipases and the amino acids representing the catalytic dyad are conserved in this protein. PLP_2.9 was overexpressed in <i>Escherichia coli</i> and the purified enzyme was characterized biochemically. PLP_2.9<i></i> hydrolyzed <i>p</i>-nitrophenyl palmitate at alkaline pH over a wide range of temperatures (55–80°C), showing high thermostability. PLP_2.9 displayed phospholipase A and acyltransferase activities on egg yolk phosphatidylcholine. Due to its high thermostability, PLP_2.9 has potential applications as a catalyst in several industrial processes.

Progress and perspectives on halide lithium conductors for all-solid-state lithium batteries

2020 ◽  
Vol 13 (5) ◽  
pp. 1429-1461 ◽  
Author(s):  
Xiaona Li ◽  
Jianwen Liang ◽  
Xiaofei Yang ◽  
Keegan R. Adair ◽  
Changhong Wang ◽  
...  
Keyword(s):  
Energy Storage ◽  
Solid State ◽  
Lithium Batteries ◽  
Electrochemical Stability ◽  
Superionic Conductors ◽  
Fundamental Understanding ◽  
Potential Applications ◽  
Synthesis Routes

This review focuses on fundamental understanding, various synthesis routes, chemical/electrochemical stability of halide-based lithium superionic conductors, and their potential applications in energy storage as well as related challenges.

Solid state fermentation of the winter savory (Satureja montana L) plant: changes of chemical composition and antimicrobial activity

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
G Juodeikiene ◽  
D Cizeikiene ◽  
A Maruška ◽  
E Bartkiene ◽  
L Basinskiene ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Solid State ◽  
Solid State Fermentation ◽  
Satureja Montana

Two Consecutive Magneto-Structural Gas-Solid Transformations with Single-Crystal Retention in Non-Porous Molecular Materials

2018 ◽  
Author(s):  
Julia Miguel-Donet ◽  
Javier López-Cabrelles ◽  
Nestor Calvo Galve ◽  
Eugenio Coronado ◽  
Guillermo Minguez Espallargas
Keyword(s):  
Solid State ◽  
Single Crystal ◽  
Structural Rearrangement ◽  
Magnetic Behaviour ◽  
Porous Solids ◽  
Potential Applications ◽  
Hcl Gas ◽  
Porous Coordination Polymer ◽  
Gas Molecules ◽  
Magnetostructural Transformation

<p>Modification of the magnetic properties in a solid-state material upon external stimulus has attracted much attention in the recent years for their potential applications as switches and sensors. Within the field of coordination polymers, gas sorption studies typically focus on porous solids, with the gas molecules accommodating in the channels. Here we present a 1D non-porous coordination polymer capable of incorporating HCl gas molecules, which not only causes a reordering of its atoms in the solid state but also provokes dramatic changes in the magnetic behaviour. Subsequently, a further solid-gas transformation can occur with the extrusion of HCl gas molecules causing a second structural rearrangement which is also accompanied by modification in the magnetic path between the metal centres. Unequivocal evidence of the two-step magnetostructural transformation is provided by X-ray single-crystal diffraction.</p>

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