scholarly journals Molecular identification and expression patterns of odorant binding protein and chemosensory protein genes inAthetis lepigone(Lepidoptera: Noctuidae)

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3157 ◽  
Author(s):  
Ya-Nan Zhang ◽  
Xiu-Yun Zhu ◽  
Ji-Fang Ma ◽  
Zhi-Ping Dong ◽  
Ji-Wei Xu ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Environmentally Friendly ◽  
Northern China ◽  
Tissue Expression ◽  
Odorant Binding Proteins ◽  
Chemosensory Protein ◽  
Genes Encoding ◽  
Odorant Binding ◽  
Lepidoptera Noctuidae ◽  
Oviposition Sites

The olfaction system of insects plays an important role in mediating various physiological behaviors, including locating hosts, avoiding predators, and recognizing mates and oviposition sites. Therefore, some key genes in the system present valuable opportunities as targets for developing novel green pesticides.Athetis lepigone, a noctuid moth can feed on more than 30 different host plants making it a serious polyphagous pest worldwide, and it has become one of the major maize pests in northern China since 2011. However, there are no reports on effective and environmentally friendly pesticides for the control of this pest. In this study, we identified 28 genes encoding putative odorant binding proteins (OBPs) and 20 chemosensory protein (CSPs) genes based on our previousA. lepigonetranscriptomic data. A tissue expression investigation and phylogenetic analysis were conducted in an effort to postulate the functions of these genes. Our results show that nearly half (46.4%) of theAlOBPsexhibited antennae-biased expression while many of theAlCSPswere highly abundant in non-antennal tissues. These results will aid in exploring the chemosensory mechanisms ofA. lepigoneand developing environmentally friendly pesticides against this pest in the future.

scholarly journals Identification and Expression Profile of Chemosensory Genes in the Small Hive Beetle Aethina tumida

Insects ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 661
Author(s):  
Lixian Wu ◽  
Xin Zhai ◽  
Liangbin Li ◽  
Qiang Li ◽  
Fang Liu ◽  
...  
Keyword(s):  
Pest Control ◽  
Expression Profiles ◽  
Environmentally Friendly ◽  
Insect Behavior ◽  
Tissue Expression ◽  
Control Measures ◽  
Aethina Tumida ◽  
Chemosensory Genes ◽  
Genes Encoding ◽  
Odorant Binding

Aethina tumida is a parasite and predator of honeybee causing severe loss to the bee industry. No effective and environmentally friendly methods are available to control this pest at present. Chemosensory genes play key roles in insect behavior which can potentially be used as targets for developing environmentally friendly pest control agents. In this study, the putative chemosensory genes in antennae and forelegs of A. tumida involved in olfaction or contact chemical communication of adults were investigated using RNA transcriptome sequencing and PCR methods. Based on transcriptomic data, unigenes encoding 38 odorant receptors (ORs), 24 ionotropic receptors (IRs), 14 gustatory receptors (GRs), 3 sensory neuron membrane proteins (SNMPs), 29 odorant binding proteins (OBPs), and 22 chemosensory proteins (CSPs) were identified. The analyses of tissue expression profiles revealed that genes encoding 38 ORs, 13 antennal IRs, 11 GRs, 1 SNMP, 24 OBPs, and 12 CSPs were predominately expressed in antennae. No significant differences in expression levels of these genes were found between males and females. Genes encoding 5 non-NMDA iGluRs, 3 GRs, 2 SNMPs, 5 OBPs, and 12 CSPs were predominately expressed in forelegs. RT-PCR assays for SNMPs, OBPs and CSPs further revealed that 3 OBPs (AtumOBP3, 26 and 28) and 3 CSPs (AtumCSP7, 8 and 21) were highly expressed in antennae. Our results enrich the gene inventory of A. tumida and facilitate the discovery of potential novel targets for developing new pest control measures.

Expression patterns of odorant-binding proteins in antennae of the moth Manduca sexta

2003 ◽  
Vol 313 (3) ◽  
pp. 321-333 ◽  
Author(s):  
James B. Nardi ◽  
Lou Ann Miller ◽  
Kimberly K. O. Walden ◽  
Susan Rovelstad ◽  
Liping Wang ◽  
...  
Keyword(s):  
Manduca Sexta ◽  
Binding Proteins ◽  
Expression Patterns ◽  
Odorant Binding Proteins ◽  
Odorant Binding

scholarly journals Genome-wide Identification and Characterization of the DELLA Subfamily in Prunus mume

2015 ◽  
Vol 140 (3) ◽  
pp. 223-232 ◽  
Author(s):  
Jiuxing Lu ◽  
Weiru Yang ◽  
Qixiang Zhang
Keyword(s):  
Expression Patterns ◽  
Ornamental Plants ◽  
Fruit Trees ◽  
Tissue Expression ◽  
Prunus Mume ◽  
Tissue Expression Analysis ◽  
Della Proteins ◽  
Genes Encoding ◽  
Positive Regulators ◽  
Delayed Flowering

Control of development is an important issue in the production of ornamental plants. Gibberellins (GAs) play a key role in regulating plant growth and development. DELLA is nuclear negative regulators of GA signaling. We identified two DELLA homologous genes, PmDELLA1 and PmDELLA2, in the genome of mei (Prunus mume) genome. We analyzed the structure, expression patterns and molecular functions of both genes. Tissue expression analysis showed that both genes were transcriptionally active. PmDELLA1 showed higher expression in seeds than PmDELLA2. This indicated that PmDELLA2 plays different roles from PmDELLA1 in seed germination. The expressions of both genes at various flowering stages were relatively low. We speculated that PmDELLAs might be positive regulators of flowering by releasing the repression of GA during floral blooming. Transgenic arabidopsis (Arabidopsis thaliana) lines overexpressing the two genes showed dwarf and delayed flowering. We confirmed that the two PmDELLAs were partially conserved with genes encoding DELLA proteins in arabidopsis. Our bioinformatics and functional analyses provide information that may be valuable to improve the economic, agronomic and ecological properties of mei and other Rosaceae fruit trees.

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