Effects of origin, seasons and storage under different temperatures on germination ofSenecio vulgaris(Asteraceae) seeds

Effects of range, seasons and storage under different temperatures on germination of Senecio vulgaris (Asteraceae) seeds

10.7287/peerj.preprints.1691 ◽

2016 ◽

Author(s):

Noel Ndihokubwayo ◽

Viet - Thang Nguyen ◽

Dandan Cheng

Keyword(s):

High Temperature ◽

Biodiversity Loss ◽

Economic Loss ◽

Storage Conditions ◽

Germination Percentage ◽

Ambient Conditions ◽

Climate Chamber ◽

Senecio Vulgaris ◽

The North ◽

And Storage

Invasive plants colonize new environments, become pests and cause biodiversity loss, economic loss and health damage. Senecio vulgaris (Common groundsel, Asteraceae), a cosmopolitan weed wildly distributes in the temperate area, is reported with large populations in the north–eastern and south–western parts, but not in southern, central, northern or north-western part of China. We studied the germination behavior of S. vulgaris to explain the distribution and the biological invasion of this species in China. We used seeds originated from 12 populations in native and invasive range (six populations in each range) to conduct germination experiments in a climate chamber and ambient condition. When incubated in climate chamber (15°C) seeds from the majority of population showed >90% germination percentage (GP) and the GP was equal for both ranges. The mean germination time (MGT) was significant different among the populations. Under ambient conditions, significant effect of range, storage conditions (stored at 4 or 27°C) and seasons (in summer or autumn) were observed on the GP while the MGT was only affected by the season. In autumn, the GP was higher (38.6%) and the MGT was slightly longer than that in summer (4.5%). In autumn, seeds stored at 4°C showed higher GP than those stored at 27°C, and seeds from invasive population revealed higher GP than those from native populations. High GP and short time for seed emergence demonstrated that in S. vulgaris seeds checked in this study were from Senecio vulgaris ssp vulgaris, the non – dormancy subspecies. The results also implied that the high temperature exceeds the threshold for S. vulgaris to germinate and storage at high temperature cause S. vulgaris seeds lost the viability greatly. This demonstrates the reason why in Wuhan S.vulgaris can’t establish natural and viable populations, and also explain why S.vulgaris is scattered in China.

Download Full-text

Effects of range, seasons and storage under different temperatures on germination of Senecio vulgaris (Asteraceae) seeds

10.7287/peerj.preprints.1691v1 ◽

2016 ◽

Author(s):

Noel Ndihokubwayo ◽

Viet - Thang Nguyen ◽

Dandan Cheng

Keyword(s):

High Temperature ◽

Biodiversity Loss ◽

Economic Loss ◽

Storage Conditions ◽

Germination Percentage ◽

Ambient Conditions ◽

Climate Chamber ◽

Senecio Vulgaris ◽

The North ◽

And Storage

Invasive plants colonize new environments, become pests and cause biodiversity loss, economic loss and health damage. Senecio vulgaris (Common groundsel, Asteraceae), a cosmopolitan weed wildly distributes in the temperate area, is reported with large populations in the north–eastern and south–western parts, but not in southern, central, northern or north-western part of China. We studied the germination behavior of S. vulgaris to explain the distribution and the biological invasion of this species in China. We used seeds originated from 12 populations in native and invasive range (six populations in each range) to conduct germination experiments in a climate chamber and ambient condition. When incubated in climate chamber (15°C) seeds from the majority of population showed >90% germination percentage (GP) and the GP was equal for both ranges. The mean germination time (MGT) was significant different among the populations. Under ambient conditions, significant effect of range, storage conditions (stored at 4 or 27°C) and seasons (in summer or autumn) were observed on the GP while the MGT was only affected by the season. In autumn, the GP was higher (38.6%) and the MGT was slightly longer than that in summer (4.5%). In autumn, seeds stored at 4°C showed higher GP than those stored at 27°C, and seeds from invasive population revealed higher GP than those from native populations. High GP and short time for seed emergence demonstrated that in S. vulgaris seeds checked in this study were from Senecio vulgaris ssp vulgaris, the non – dormancy subspecies. The results also implied that the high temperature exceeds the threshold for S. vulgaris to germinate and storage at high temperature cause S. vulgaris seeds lost the viability greatly. This demonstrates the reason why in Wuhan S.vulgaris can’t establish natural and viable populations, and also explain why S.vulgaris is scattered in China.

Download Full-text

Seed storage behaviour of Buxus wallichiana Baillon: An important woodcraft species of Indian Himalayan region

Journal of Applied and Natural Science ◽

10.31018/jans.v11i2.2045 ◽

2019 ◽

Vol 11 (2) ◽

pp. 311-314

Author(s):

Manisha Thapliyal ◽

Pravin Rawat

Keyword(s):

Room Temperature ◽

Seed Storage ◽

Storage Conditions ◽

Germination Percentage ◽

Himalayan Region ◽

Germination Test ◽

Storage Duration ◽

Indian Himalayan Region ◽

Ambient Room Temperature ◽

Forest Division

Seeds of Buxus wallichiana Baillon are dormant and not much information is available on their shelf life under storage conditions. Therefore, this study was conducted to investigate the seed storage behaviour of B. wallichiana, an important woodcraft species of the Indian Himalayan Region. Healthy matured fruits of B. wallichiana were collected from Matkangra block, Chakrata Forest Division of Uttarakhand. The seeds were extracted, cleaned, processed manually and their initial viability was tested through germination test. After initial viability determination, seeds were processed and placed in air-tight plastic boxes for storage at four temperatures viz., ambient room temperature (Control), 5°C, 15°C and -20°C. Thereafter, at every two months interval, germination test was conducted to assess the viability of the stored seeds. The results revealed that the initial viability of freshly collected seeds was 26.66 per cent and it took 7.83 days to complete germination. After two months of storage the germination percentage increased in the seeds stored at all the temperatures going up to maximum (73.33%) in the ones stored at -20°C followed by 15°C (66.67%) as compared to 50% in the seeds stored at room temperature. Similarly, the mean germination time also increased proportionally, longest being 44 days for seeds stored at -20°C. Seeds stored at ambient room temperature lost viability completely at 6 months storage duration while almost half the viability was lost in seeds stored at 15°C (36.67%) and -20°C (26.67%). From present study, it can be concluded that the longevity of the B. wallichiana seeds is around 12 months when the seeds are stored at lower temperatures, maintaining the viability about 23% at 5°C and 15°C and 16.67 % at -20°C.

Download Full-text

Effect of storage conditions and sodium hypochlorite treatment on germination of Cucumis prophetarum (Cucurbitaceae) seeds from arid Arabian deserts

10.21203/rs.3.rs-201765/v1 ◽

2021 ◽

Author(s):

Hatem Ahmed Shabana ◽

Tamer Mahmoud ◽

Sanjay Gairola ◽

Asma Al Ketbi ◽

Mariam Aljasmi ◽

...

Keyword(s):

Seed Germination ◽

United Arab Emirates ◽

Sodium Hypochlorite ◽

Room Temperature ◽

Seedling Emergence ◽

Seed Storage ◽

Storage Conditions ◽

Germination Percentage ◽

Continuous Darkness ◽

Temperature Requirements

Abstract Background This is the first study on seed germination of Cucumis prophetarum in the Arabian Peninsula. Light and temperature as the main environmental factors and seed storage conditions greatly affect the germination of many Cucurbitaceae species. C. prophetarum is the perennial prostrate with woody rootstocks that grow throughout the year in the arid Arabian deserts. We examined the effects of seed storage conditions and sterilization by sodium hypochlorite (NaOCL) on germination of C. prophetarum seeds. Matured, fresh and field stored seeds were collected in March of 2016 and 2017 from a population in the United Arab Emirates. Fresh and the stored seeds (field, freeze, and room temperature) were germinated at three temperature regimes (15/25, 20/30 and 25/35°C) in both continuous darkness and alternating 12 h light/12 h darkness. Results The fresh and freeze stored seeds had higher germination percentage than the field and room temperature stored seeds. High germination was recorded in both lower and moderate temperatures (15/25 and 20/30°C) in light conditions. Sterilization of the seeds by NaOCl had a negative effect on the speed and germination percentage under all storage conditions, and it changes light and temperature requirements of seeds for germination. Conclusions Germination of C. prophetarum is sensitive to incubation light and temperature as well as to the seed storage conditions. The germination ability of stored seeds indicates that seed storage behaviour of C. prophetarum is orthodox. Light and temperature requirements for seed germination reflects a suitable place, habitat, and time for seedling emergence of C. prophetarum. The effect of NaOCl treatment on the germination may be associated with concentration and exposure periods of seeds. Therefore, we suggest that when using NaOCl for seed sterilization or to stimulate the germination, caution must be taken for using its concentration and exposure time, especially for species with thin and soft seed coats like C. prophetarum. Further studies on seed germination ecology would help to understand better the adaptive strategy of the C. prophetarum in the arid desert environments.

Download Full-text

Oxidative Stability at Different Storage Conditions and Adulteration Detection of Prickly Pear Seeds Oil

Journal of Food Quality ◽

10.1155/2020/8837090 ◽

2020 ◽

Vol 2020 ◽

pp. 1-12

Author(s):

Fatima Ettalibi ◽

Abderraouf El Antari ◽

Chemseddoha Gadhi ◽

Hasnaâ Harrak

Keyword(s):

Fatty Acids ◽

Oxidative Stability ◽

Room Temperature ◽

Negative Impact ◽

Storage Conditions ◽

Prickly Pear ◽

Economic Losses ◽

Fatty Acids Composition ◽

Quality Aspects ◽

The Impact

Lipid oxidation and adulteration have a negative impact on functionality and notoriety of foods especially vegetable oils and cause economic losses. The present study investigates the control of two commercial quality aspects of prickly pear seeds oil (PPSO): oxidative stability during storage and detection of adulteration. Peroxide index, specific extinction coefficients K232 and K270, free acidity, and fatty acids composition were evaluated during different periods of incubation (6, 12, and 18 months) at various temperatures (4°C, 25°C, 40°C, and uncontrolled room temperature ranging between 4°C and 40°C) with different packaging (protected and unprotected from sunlight, with and without nitrogen gas bubbling). Based on the physicochemical and biochemical parameters evolution, this study has shown that PPSO stored at 4°C for 18 months preserves the initial quality. However, at 40°C, an intense lipid oxidative process occurred after 6 months of storage. The changes have also affected fatty acids composition, especially rates of linoleic and oleic acids. The shelf-life of oils stored at 25°C and at uncontrolled room temperature can be limited to 6 months. Regarding the impact of light and nitrogen bubbling, sunlight has affected seriously the oxidative stability of oils after 12 months of storage and the bubbling with nitrogen has improved their stability when they have been stored in clear glass bottles. The levels of adulteration detection using fatty acids as markers are relatively high. The detection of oil adulteration can be depicted by fatty acids composition up to 15% of olive and almond oils and up to 20% of rapeseed oil. The iodine value could also be an indicator of the sunflower oil presence in PPSO. Therefore, other minor compounds including sterols and tocopherols should be investigated to depict PPSO adulteration with cheaper oils and to determine lower levels of detection in order to ensure the authenticity of PPSO.

Download Full-text

Salinity Tolerance of Black Gram Cultivars During Germination and Early Seedling Growth

Cercetari Agronomice in Moldova ◽

10.2478/cerce-2018-0025 ◽

2018 ◽

Vol 51 (3) ◽

pp. 51-68 ◽

Cited By ~ 1

Author(s):

M.K. Hasan ◽

M.S. Islam ◽

M.R. Islam ◽

H.N. Ismaan ◽

A. El Sabagh

Keyword(s):

Salt Stress ◽

Salinity Tolerance ◽

Seedling Growth ◽

Negative Impact ◽

Germination Rate ◽

Dry Weight ◽

Germination Percentage ◽

Shoot Length ◽

Sand Culture ◽

Black Gram

Abstract A laboratory experiment regarding germination and seedling growth test was conducted with three black gram genotypes tested under three salinity levels (0, 75 and 150 mM), for 10 days, in sand culture within small plastic pot, to investigate the germination and seedling growth characteristics. Different germination traits of all black gram genotypes, like germination percentage (GP), germination rate (GR), coefficient of velocity of germination (CVG) greatly reduced, as well as mean germination time (MGT) increased with increasing salt stress. At high salt stress, BARI Mash-3 provided the highest GP reduction (28.58%), while the lowest was recorded (15.79% to control) in BARI Mash-1. Salinity have the negative impact on shoot and root lengths, fresh and dry weights. The highest (50.32% to control) and lowest reduction (36.39%) of shoot length were recorded in BARI Mash-2 and BARI Mash-1, respectively, under 150 mM NaCl saline conditions. There were significant reduction of root lengths, root fresh and dry weight, shoot length, shoot fresh and dry weight in all genotypes under saline condition. The genotypes were arranged as BARI Mash-1 > BARI Mash-3 > BARI Mash-2, with respect to salinity tolerance.

Download Full-text

Enhanced preservation of vacuum-packaged Atlantic salmon by hyperbaric storage at room temperature versus refrigeration

Scientific Reports ◽

10.1038/s41598-021-81047-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Liliana G. Fidalgo ◽

Mário M. Q. Simões ◽

Susana Casal ◽

José A. Lopes-da-Silva ◽

Ivonne Delgadillo ◽

...

Keyword(s):

Atlantic Salmon ◽

Microbial Activity ◽

Room Temperature ◽

Energy Savings ◽

Textural Properties ◽

Storage Conditions ◽

Volatile Profile ◽

Fragmentation Index ◽

C 30 ◽

Water Holding

AbstractHyperbaric storage at room temperature (HS/RT: 75 MPa/25 °C) of vacuum-packaged fresh Atlantic salmon (Salmo salar) loins was studied for 30 days and compared to atmospheric pressure at refrigerated temperatures (AP/5 °C, 30 days) and RT (AP/25 °C, 5 days). Most of the fatty acids were not affected by storage conditions, with only a slight decrease of docosahexaenoic acid (DHA) content (n-3 polyunsaturated fatty acid) for AP samples, reflected in the lower polyene index values obtained and higher oxidation extent. For HS, a lower lipid oxidation extension and a slower increase of myofibrillar fragmentation index values were observed, when compared to AP samples. The volatile profile was similar for the HS and fresh samples, with the HS samples retaining fresh-like alcohols and aldehydes components, which disappeared in AP samples, mainly in AP/25 °C samples. The volatile profile for AP samples (5 and 25 °C) revealed mostly spoilage-like compounds due to microbial activity. Drip loss increased progressively during the 30 days of storage under HS, while a slight decrease of water holding capacity after 5 days was observed, increasing further after 30 days. Regarding textural properties, only resilience was affected by HS, decreasing after 30 days. So, HS/RT could represent an interesting extended preservation methodology of fresh salmon loins, since allows retaining important physicochemical properties for at least 15 days, while refrigeration after 5 days showed already volatile spoilage-like compounds due to microbial activity. Furthermore, this methodology allows additional considerable energy savings when compared to refrigeration.

Download Full-text

Impact of Thermotherapy and Short-Term Storage on Quercus robur L. Acorn Mycobiota and Germination

Forests ◽

10.3390/f12050528 ◽

2021 ◽

Vol 12 (5) ◽

pp. 528

Author(s):

Jelena Kranjec Orlović ◽

Damir Drvodelić ◽

Marko Vukelić ◽

Matea Rukavina ◽

Danko Diminić ◽

...

Keyword(s):

Natural Regeneration ◽

Fungal Diversity ◽

Quercus Robur ◽

Negative Impact ◽

Storage Conditions ◽

Short Term ◽

Isolation Frequency ◽

Short Term Storage ◽

Penicillium Spp ◽

Term Storage

When natural regeneration of Quercus robur stands is hampered by an insufficient acorn yield, human assisted sowing of acorns collected in non-affected stands and stored for some period of time is performed. To inhibit the development of fungi and acorn deterioration during storage, thermotherapy is usually applied by submerging acorns for 2.5 h in water heated to 41 °C. This research aimed to test the effect of four thermotherapy treatments of different durations and/or applied temperatures as well as short-term storage at −1 °C or 3 °C on acorn internal mycobiota and germination. Fungal presence in cotyledons was analyzed in 450 acorns by isolation of mycelia on artificial media, followed by a DNA-based identification. Germination of 2000 acorns was monitored in an open field trial. Thermotherapy significantly decreased fungal diversity, while storage at 3 °C increased the isolation frequency of several fungi, mainly Penicillium spp. The most frequently isolated fungi did not show a negative impact on acorn germination after short-term storage. The study confirmed the efficiency of thermotherapy in the eradication of a part of acorn internal mycobiota, but also its effect on the proliferation of fast-colonizing fungi during storage. However, the latter showed to be more stimulated by storage conditions, specifically by storage at 3 °C.

Download Full-text

Characteristics of Packaged Water under Different Storage Conditions Within Osogbo Metropolis

UNIOSUN Journal of Engineering and Environmental Sciences ◽

10.36108/ujees/0202.20.0221 ◽

2020 ◽

Vol 2 (2) ◽

Author(s):

O.O Fadipe

Keyword(s):

Room Temperature ◽

Control Unit ◽

Storage Conditions ◽

Prolonged Storage ◽

E Coli ◽

Physico Chemical ◽

Routine Basis ◽

Regulatory Bodies ◽

Microbiological Analyses ◽

Sachet Water

The study investigated the characteristics of packaged water stored under ambient and sunlight conditions. This is with a view to testing the effect of prolonged storage under different storage conditions on its quality. In addition it analyzed the interactions between the parameters. Two packs each of bottled and sachet water was purchased from each factory at the point of production and ready for distribution to wholesalers. Twenty eight pieces of packaged water from each factory were kept at room temperature and the same quantity were kept under sunlight. Physico-chemical and microbiological analyses were carried out on the remaining packaged water within 24hrs. Half of the samples stored at the two storage conditions were removed for analysis at 3 weeks while the remaining half was analyzed after 6weeks. The physico-chemical characteristics were within the WHO recommended values except for the pH of some samples that have values in the acidic range of 6.2-6.48. All the physico-chemical values increased for samples kept under sunlight. All the water samples showed growth in faecal coliform (4-46 cfu/100 mL) and E. coli (0-13 cfu/100 mL) for samples kept under sunlight at three weeks and this growth increased to the sixth week. The presence of E. coli is an indication that the packaged water is not pure. Displaying packaged water under the sunlight and storing beyond 3 weeks by vendors have effect on the potability of the product. The regulatory bodies should raise awareness and ensure manufacturer have a quality control unit to test on a routine basis.

Download Full-text

Abstract P189: Method of Cold Saline Storage for Prehospital Induced Hypothermia

Circulation ◽

10.1161/circ.118.suppl_18.s_1486-a ◽

2008 ◽

Vol 118 (suppl_18) ◽

Author(s):

Mitch Kampmeyer ◽

Clifton W Callaway

Keyword(s):

Room Temperature ◽

Prehospital Care ◽

Rank Test ◽

Induced Hypothermia ◽

Ambient Conditions ◽

Storage Unit ◽

Cold Saline ◽

Kaplan Meier ◽

Significant Difference ◽

Ambient Room Temperature

Recent research supports the use of cold IV fluid as a method for initiating therapeutic hypothermia in post-cardiac arrest resuscitation. However, prehospital care programs employing this treatment have encountered various difficulties. Barriers to prehospital induced hypothermia protocols include the lack of effective or economically reasonable methods to maintain cold saline in the field. Objective. Determine the time that a standard commercial cooler can maintain two 1-liter normal saline solution (NSS) bags below 4°C in 3 different environments. Methods. Environments simulating an ambulance compartment were created for the experiment. NSS temperatures were continuously recorded inside a standard commercial cooler with or without ice packs (IPs) under one of three scenarios: ambient room temperature (25°C) without (IPs), ambient room temperature with IPs and 50°C ambient temperature with IPs. Four trials under each condition were performed. Time to warm to 4°C was compared using Kaplan-Meier log rank test. Results. In a room temperature environment with IPs, the NSS warmed to 4°C in a mean interval of 29 hrs 53 mins versus in ambient room temperature without IPs (1 hr 21 mins) versus in constant hot environment of 50°C with IPs (10 hrs 50 mins). A significant difference was found between the three environments (log-rank =17.90, dF =2, p =0.0001). Conclusions. Low technology methods in the form of a cooler and IPs can provide cold NSS storage for longer than a full 24 hour shift in a room temperature ambulance. In hot ambient conditions, 4°C NSS can be maintained for nearly 11 hours using this method. This model exhibits an economical, easily deployable cold saline storage unit.

Download Full-text