scholarly journals Isolation and characterization of 10 polymorphic microsatellite loci for the endangered Galapagos-endemic whitespotted sandbass (Paralabrax albomaculatus)

PeerJ ◽  
2015 ◽  
Vol 3 ◽  
pp. e1253 ◽  
Author(s):  
Alicia C. Bertolotti ◽  
Sarah M. Griffiths ◽  
Nathan K. Truelove ◽  
Stephen J. Box ◽  
Richard F. Preziosi ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Marine Reserve ◽  
Iucn Red List ◽  
Closely Related Species ◽  
Important Species ◽  
Isolation And Characterization ◽  
Commercially Important ◽  
Paired End Sequencing ◽  
Galapagos Marine Reserve

The white-spotted sandbass (Paralabrax albomaculatus)is a commercially important species in the Galapagos Marine Reserve, but is classified as endangered in the IUCN Red List. For this study, 10 microsatellite loci were isolated and characterized using Illumina paired-end sequencing. These loci can be used for genetic studies of population structure and connectivity to aid in the management of the white-spotted sandbass and other closely-related species. The 10 characterized loci were polymorphic, with 11–49 alleles per locus, and observed heterozygosity ranged from 0.575 to 0.964. This set of markers is the first to be developed for this species.

scholarly journals Next Generation Sequencing, Isolation and Characterization of 14 Microsatellite Loci of Canthon Cyanellus (Coleoptera: Scarabaeidae)

2021 ◽  
Author(s):  
Luis R. Arce-Valdés ◽  
Rosa Ana Sánchez-Guillén ◽  
Janet Nolasco-Soto ◽  
Mario E. Favila
Keyword(s):  
Next Generation Sequencing ◽  
Microsatellite Loci ◽  
Dung Beetle ◽  
Distribution Range ◽  
Next Generation ◽  
Isolation And Characterization ◽  
Paired End Sequencing ◽  
Generation Sequencing

Abstract BackgroundWe used Illumina paired-end-sequencing to isolate and characterize microsatellites of Canthon cyanellus, a Neotropical roller dung beetle encompassing several lineages within its distribution range.Methods and ResultsWe examined C. cyanellus specimens collected at eight different localities in Mexico (two or three specimens per locality). We initially made amplification tests with 16 loci, but two of these showed amplification problems; thus, we evaluated 14 loci. The 14 microsatellites were polymorphic, with 2–16 alleles each. The expected and observed heterozygosity ranged from 0.11 to 0.76 and from 0.20 to 0.78, respectively. ConclusionsThese microsatellites will help to assess structure at the population and lineage levels, identify zones of potential hybridization between lineages, and draw a more precise geographic delimitation of Canthon cyanellus lineages.

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization

Isolation and characterization of thirteen novel dinucleotide microsatellite loci from Tetraena mongolica Maxim

2014 ◽  
Vol 6 (2) ◽  
pp. 297-299 ◽  
Author(s):  
YingBiao Zhi ◽  
Ling Ding ◽  
Yupeng Wang ◽  
Longfei Hu ◽  
Yupeng Hua ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Microsatellite Loci of Clarias macrocephalus and Their Application to Genetic Diversity Study

1999 ◽  
Vol 65 (4) ◽  
pp. 520-526 ◽  
Author(s):  
Uthairat Na-Nakorn ◽  
Nobuhiko Taniguchi ◽  
Estu Nugroho ◽  
Shingo Seki ◽  
Wongpathom Kamonrat
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Loci ◽  
Isolation And Characterization ◽  
Clarias Macrocephalus ◽  
Genetic Diversity Study ◽  
Diversity Study

Isolation and characterization of microsatellite loci inCoccoloba cereifera(Polygonaceae), an endangered species endemic to the Serra do Cipó, Brazil

2008 ◽  
Vol 8 (4) ◽  
pp. 854-856 ◽  
Author(s):  
RENNAN G. MOREIRA ◽  
ROSS A. MCCAULEY ◽  
AUREA C. CORTÉS-PALOMEC ◽  
M. BERNADETE LOVATO ◽  
G. WILSON FERNANDES ◽  
...  
Keyword(s):  
Endangered Species ◽  
Microsatellite Loci ◽  
Serra Do Cipó ◽  
Isolation And Characterization
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