scholarly journals Evaluation of structural and ultrastructural changes in thyroid and parathyroid glands after near infrared irradiation: study on an animal model

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11891
Author(s):  
Carlos Serra ◽  
Luis Silveira
Keyword(s):  
Electron Microscopy ◽  
Near Infrared ◽  
Secretory Granules ◽  
Parathyroid Glands ◽  
Infrared Light ◽  
Ultrastructural Changes ◽  
Pathological Examination ◽  
Infrared Irradiation ◽  
Technical Problems ◽  
Cervical Incision

Intraoperative identification of parathyroid glands is a tough task for surgeons performing thyroid or parathyroid surgery, because the small size, color and shape of these glands hinder their discrimination from other cervical tissues. In 2011, Paras described the autofluorescence of parathyroid glands, a property that could facilitate their intraoperative identification. Parathyroid glands submitted to a 785 nm laser beam emit fluorescence in the near infrared range, with a peak at 822 nm. As the intrinsic properties of secretory tissues may be affected by the exposure to the near infrared light, a situation that could preclude their intraoperative utilization, the authors compared the structural and ultra-structural patterns of rat’s thyroid and parathyroid glands submitted to irradiation replicating the conditions that allow their intraoperative identification, with those of non irradiated animals. Twenty-four Wistar rats were divided into six groups: animals of Groups 1, 3 and 5 were submitted under general anesthesia to direct irradiation of the cervical area with a 780 nm LED light for 3 minutes through a cervical incision, and animals of Groups 2, 4 and 6 were submitted to cervical dissection without irradiation. Animals of were euthanized immediately (Groups 1 and 2), at Day 30 (Groups 3 and 4) at and at Day 60 (Groups 5 and 6) and thyroid and parathyroid glands were removed: one lobe was prepared for conventional pathological examination and the other lobe for electron microscopy observed by three experienced pathological experts. Twenty-four samples were prepared for conventional histology and there were no alterations reported in any group. Due to technical problems, only 21 samples were observed by electron microscopy and there were no differences in the ultrastructure of parathyroid and thyroid glands, namely the nuclear pattern, mitochondria, endoplasmic reticulum or secretory granules, in any of the groups. These results confirm the innocuity of near infrared irradiation’, allowing its intraoperative utilization.

Transferrin-coated magnetic upconversion nanoparticles for efficient photodynamic therapy with near-infrared irradiation and luminescence bioimaging

Nanoscale ◽  
2017 ◽  
Vol 9 (31) ◽  
pp. 11214-11221 ◽  
Author(s):  
Dan Wang ◽  
Lin Zhu ◽  
Yuan Pu ◽  
Jie-Xin Wang ◽  
Jian-Feng Chen ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Magnetic Force ◽  
Near Infrared ◽  
Infrared Light ◽  
Upconversion Nanoparticles ◽  
Infrared Irradiation ◽  
Near Infrared Light

A novel platform based on transferrin coated magnetic upconversion nanoparticles was developed for near-infrared light induced imaging and photodynamic therapy with enhanced efficiency by magnetic force.

Increased identification of parathyroid glands using near infrared light during thyroid and parathyroid surgery

2017 ◽  
Vol 31 (9) ◽  
pp. 3737-3742 ◽  
Author(s):  
Jorge Falco ◽  
Fernando Dip ◽  
Pablo Quadri ◽  
Martin de la Fuente ◽  
Marcos Prunello ◽  
...  
Keyword(s):  
Near Infrared ◽  
Parathyroid Glands ◽  
Infrared Light ◽  
Parathyroid Surgery ◽  
Near Infrared Light

Plasmon-induced charge separation at the interface between ITO nanoparticles and TiO2 under near-infrared irradiation

2019 ◽  
Vol 21 (10) ◽  
pp. 5674-5678 ◽  
Author(s):  
Seung Hyuk Lee ◽  
Hiroyasu Nishi ◽  
Tetsu Tatsuma
Keyword(s):  
Charge Separation ◽  
Near Infrared ◽  
Electron Injection ◽  
Infrared Light ◽  
Infrared Irradiation ◽  
Near Infrared Light ◽  
Induced Charge ◽  
Ito Nanoparticles

Plasmon-induced charge separation by continuous electron injection was achieved from ITO nanoparticles to TiO2 under 1500–2200 nm near-infrared light.

scholarly journals Up-Conversion Nanomaterial Doped With Au Nanoparticles With Photothermal Conversion for Multi-Modality Imaging

2021 ◽  
Author(s):  
Wei Zhang ◽  
Yanli Lu ◽  
Yang Zang ◽  
Qingyun Xiong ◽  
Jinping Xiong
Keyword(s):  
Electron Microscopy ◽  
Gold Nanoparticles ◽  
Near Infrared ◽  
Au Nanoparticles ◽  
Cell Counting ◽  
Infrared Light ◽  
Transmission Electron ◽  
Before And After ◽  
Low Cytotoxicity ◽  
Magnetic Resonance Imaging Mri

In this study, a new method for synthesizing Au-NaYF4:Yb3+/Er3+-DSPE-PEG2K nanocomposites was introduced. Using a hydrothermal method, the synthesized Yb3+- and Er3+-codoped NaYF4 upconversion luminescent materials and Au nanoparticles were doped into upconversion nanomaterials and modified with DSPE-PEG2k up-conversion nanomaterials. This material is known as Ag-UCNPs-DSPE-PEG2k, it improves both the luminous intensity because of the doped Au nanoparticles and has low cytotoxicity because of the DSPE-PEG2k modified. Exciting UCNPs with a wavelength of 980nm near-infrared light will emit light with a wavelength of 520nm to further excite gold nanoparticles to convert light energy into heat. Successful synthesized gold nanoparticles was confirmed using transmission electron microscopy (TEM). The morphology of UCNPs was observed using scanning electron microscopy (SEM), and the mapping confirmed the successful doping of Au nanoparticles. Fluorescence spectra were used to compare changes in luminescence intensity before and after doping Au nanoparticles. The cytotoxicity of Au-UCNPs-DSPE-PEG2K was tested via the cell counting kit-8 (CCK-8) method, and its imaging ability was characterized using the Magnetic Resonance Imaging (MRI) and Computed Tomography (CT) method.

Solvothermal Synthesis and Near-Infrared Shielding Properties of Cs0.3WO3/WO3 Composites

2020 ◽  
Vol 19 (04) ◽  
pp. 1950032
Author(s):  
Zhao Linyuan ◽  
Yang Mingqing ◽  
Lv Yong
Keyword(s):  
Electron Microscopy ◽  
Near Infrared ◽  
Raw Materials ◽  
Tungsten Bronze ◽  
Tungstic Acid ◽  
Light Transmittance ◽  
Infrared Light ◽  
Transmission Electron ◽  
Shielding Properties ◽  
Electron Energy Loss

The Cs[Formula: see text]WO3/WO3 composite with near-infrared shielding properties was synthesized by the solvothermal method using tungstic acid and cesium salt as raw materials. The as-prepared composites were tested by X-ray powder diffraction, scanning electron microscopy, energy spectrum analysis, transmission electron microscopy, electron energy loss spectroscopy, and ultraviolet-visible near-infrared spectroscopy. The effects of different reaction conditions on the structure and near-infrared shielding properties of the synthesized composites were investigated. The best near-infrared light transmittance of as-prepared composites can reach up to 9%, which provides a feasible solution for the near-infrared shielding material. The new homogeneous composites of cesium tungsten bronze and tungsten oxide are good candidates for solar filters.

Plasmonic behaviour and plasmon-induced charge separation of nanostructured MoO3−x under near infrared irradiation

Nanoscale ◽  
2018 ◽  
Vol 10 (6) ◽  
pp. 2841-2847 ◽  
Author(s):  
Seung Hyuk Lee ◽  
Hiroyasu Nishi ◽  
Tetsu Tatsuma
Keyword(s):  
Charge Separation ◽  
Near Infrared ◽  
Infrared Light ◽  
Infrared Irradiation ◽  
Near Infrared Light ◽  
Induced Charge

Plasmonic MoO3−x nanostructures were developed on template nanoparticles and plasmon-induced charge separation was performed under near infrared light at the interface between the nanostructure and TiO2.

Ultrastructural modifications provoked by tamoxifen either alone or combined with oestradiol in the uteri of fetal or newborn guinea-pigs

1986 ◽  
Vol 110 (2) ◽  
pp. 197-NP ◽  
Author(s):  
J. R. Pasqualini ◽  
F. Lecerf
Keyword(s):  
Electron Microscopy ◽  
Epithelial Cells ◽  
Guinea Pigs ◽  
Secretory Granules ◽  
Perinatal Period ◽  
Ultrastructural Changes ◽  
Uterine Epithelial Cells ◽  
Transmission Electron ◽  
Uterine Growth ◽  
Newborn Animals

ABSTRACT The ultrastructural changes in the uterine epithelial cells of fetal and newborn guinea-pigs were examined by transmission electron microscopy after the administration of tamoxifen, oestradiol or a combination of tamoxifen and oestradiol. Tamoxifen provoked a substantial alteration of the mitochondria and the rough endoplasmic reticulum with the formation of numerous vacuoles and secretory granules, indicating an enhancement of synthetic and secretory activities. The mixture of oestradiol and tamoxifen increased the effect and most organelles became irreversibly altered, particularly in the uteri of newborn animals after a long treatment. It is concluded that, in addition to the agonistic oestrogen effects of tamoxifen on uterine growth, DNA and protein contents, tamoxifen also acts as an agonist on the ultrastructural changes of the uterine epithelial cells during the perinatal period of the guinea-pig. J. Endocr. (1986) 110, 197–202

scholarly journals Near-infrared autofluorescence imaging to detect parathyroid glands in thyroid surgery

2018 ◽  
Vol 100 (1) ◽  
pp. 33-36 ◽  
Author(s):  
R Ladurner ◽  
N Al Arabi ◽  
U Guendogar ◽  
KKJ Hallfeldt ◽  
H Stepp ◽  
...  
Keyword(s):  
Visual Recognition ◽  
Near Infrared ◽  
Parathyroid Tissue ◽  
Parathyroid Glands ◽  
Surrounding Tissue ◽  
Infrared Light ◽  
Autofluorescence Imaging ◽  
Central Lymph Node Dissection ◽  
Blue Channel ◽  
Central Lymph

ObjectiveTo identify and save parathyroid glands during thyroidectomy by displaying their autofluorescence.MethodsAutofluorescence imaging was carried out during thyroidectomy with and without central lymph node dissection. After visual recognition by the surgeon, the parathyroid glands and the surrounding tissue were exposed to near-infrared light with a wavelength of 690–770 nm using a modified Karl Storz near infrared/indocyanine green endoscopic system. Parathyroid tissue was expected to show near infrared autofluorescence at 820 nm, captured in the blue channel of the camera.ResultsWe investigated 41 parathyroid glands from 20 patients; 37 glands were identified correctly based on near-infrared autofluorescence. Neither lymph nodes nor thyroid revealed substantial autofluorescence and nor did adipose tissue.ConclusionsParathyroid tissue is characterised by showing autofluorescence in the near-infrared spectrum. This effect can be used to identify and preserve parathyroid glands during thyroidectomy.

Ultrastructural Changes of Canine Kidneys During 24-Hour Perfusion on a LI-400 Preservation System

1971 ◽  
Vol 29 ◽  
pp. 316-317
Author(s):  
M. O. Magnusson ◽  
D. G. Osborne ◽  
T. Shimoji ◽  
W. S. Kiser ◽  
W. A. Hawk
Keyword(s):  
Electron Microscopy ◽  
Electrolyte Solution ◽  
Ultrastructural Changes ◽  
Midline Incision ◽  
Short Term ◽  
Pentobarbital Anesthesia ◽  
Pulsatile Perfusion

Short term experimental and clinical preservation of kidneys is presently best accomplished by hypothermic continuous pulsatile perfusion with cryoprecipitated and millipore filtered plasma. This study was undertaken to observe ultrastructural changes occurring during 24-hour preservation using the above mentioned method.A kidney was removed through a midline incision from healthy mongrel dogs under pentobarbital anesthesia. The kidneys were flushed immediately after removal with chilled electrolyte solution and placed on a LI-400 preservation system and perfused at 8-10°C. Serial kidney biopsies were obtained at 0-½-1-2-4-8-16 and 24 hours of preservation. All biopsies were prepared for electron microscopy. At the end of the preservation period the kidneys were autografted.

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