scholarly journals Novel sampling methods for monitoring Anopheles arabiensis from Eritrea

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11497
Author(s):  
Jacques D. Charlwood ◽  
Amanuel Kidane Andegiorgish ◽  
Yonatan Estifanos Asfaha ◽  
Liya Tekle Weldu ◽  
Feven Petros ◽  
...  

Background Studies comparing novel collection methods for host seeking and resting mosquitoes A. arabiensis were undertaken in a village in Eritrea. Techniques included an odor baited trap, a novel tent-trap, human landing collection and three methods of resting collection. A technique for the collection of mosquitoes exiting vegetation is also described. Pre-gravid rates were determined by dissection of host seeking insects and post-prandial egg development among insects collected resting. Results Overall 5,382 host-seeking, 2,296 resting and 357 A. arabiensis exiting vegetation were collected. The Furvela tent-trap was the most efficient, risk-free method for the collection of outdoor host-seeking insects, whilst the Suna trap was the least effective method. Mechanical aspirators (the CDC backpack or the Prokopack aspirator) were superior to manual aspiration in a dark shelter but there was no advantage over manual aspiration in a well-lit one. An estimated two-thirds of newly-emerged mosquitoes went through a pre-gravid phase, feeding twice before producing eggs. Mosquitoes completed gonotrophic development in a dark shelter but left a well-lit shelter soon after feeding. One blood-fed female marked in the village was recaptured 2 days after release exiting vegetation close to the oviposition site and another, shortly after oviposition, attempting to feed on a human host 3 days after release. Exit rates of males from vegetation peaked 3 min after the initial male had left. Unfed and gravid females exited approximately 6 min after the first males. Conclusions Furvela tent-traps are suitable for the collection of outdoor biting A. arabiensis in Eritrea whilst the Prokopack sampler is the method of choice for the collection of resting insects. Constructing well-lit, rather than dark, animal shelters, may encourage otherwise endophilic mosquitoes to leave and so reduce their survival and hence their vectorial capacity.

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Stella T. Kessy ◽  
Bruno A. Nyundo ◽  
Ladslaus L. Mnyone ◽  
Issa N. Lyimo

Despite the considerable progress made so far, the effectiveness and mass application of odour-baited outdoor mosquito control devices in pipelines is limited by several factors. These include the design and size of the devices, optimal placement of attractive blends, and nature of materials into which the blends are impregnated. The primary aim of this study was to manipulate these factors to improve the attractiveness of our recently developed passive outdoor host seeking device (POHD) to outdoor biting Anopheles arabiensis. Specifically, the study aimed to determine optimal placement of odour blends and killing bioactives in POHD for maximum attraction and killing of An. arabiensis and to assess the effects of blend types, formulation, and residual activity on attractiveness of the POHD to An. arabiensis. The POHDs baited with attractive blends, carbon dioxide (CO2), and bendiocarb-treated electrostatic netting were placed either towards the top or bottom openings, and other modifications were exposed to An. arabiensis under the semifield system at Ifakara Health Institute (IHI). Each night, a total of 100 starved female, 3–7-day-old, semifield reared An. arabiensis mosquitoes were released, collected the next morning (alive or dead), counted, and recorded. Live mosquitoes were maintained in the semifield insectary and monitored for 24 hours mortality. Each treatment combination of the POHD was tested in three replicates. Overall, the results indicated that the proportion of mosquitoes attracted to and killed in the POHD varied with position of attractants and killing agent (bendiocarb). The POHD with bottom placed attractants and bendiocarb attracted and killed higher proportion of mosquitoes compared to the POHD with top placed attractants and bendiocarb. The highest mortalities were observed when the POHD was baited with a combination of attractive blends and CO2. Moreover, the residual activity of attractive blends applied inside POHD varied with type and formulation of attractive blend. The POHD packed with Mbita and Ifakara blend in microencapsulated pellets (granules) attracted higher proportion of mosquitoes than that baited with soaked nylon-strip formulation of either blends. Interestingly, POHD baited with Mbita blend in microencapsulated pellets (granules) formulation attracted and killed higher proportion of mosquitoes (>90%) than that baited with Ifakara blend even 9 months after application. Conclusively, the POHD remained effective for a relatively longer period of time when baited with bottom placed synthetic blends and CO2 combination, thus warranting further trials under real life situations.


Tata Kelola ◽  
2020 ◽  
Vol 7 (1) ◽  
pp. 28-37
Author(s):  
Rudi Rudi ◽  
Abdul Rahman Mus ◽  
Serlin Serang

Penelitian ini bertujuan untuk mengetahui pengaruh faktor Lingkungan Kerja, Kompensasi dan Kemampuan Kerja secara simultan terhadap kinerja pegawai dan mengetahui faktor yang paling dominan terhadap kinerja pegawai di Kelurahan Baju Bodoa Kecamatan Maros Baru Kabupaten Maros. Peneltian ini menggunakan metode sampel dan metode pengumpulan data, data diperoleh dengan cara observasi, wawancara, kuesioner dan dokumentasi, sampel dalam penelitian ini berdasarkan pada metode Non Probability Sampling. Teknik Non Probability Sampling yang dipilih yaitu dengan Sampling Jenuh (sensus) yaitu metode penarikan sampel bila semua anggota populasi dijadikan sampel. Hasil dari pengujian menunjukkan bahwa secara simultan ketiga variabel berpengaruh signifikan terhadap Kinerja Pegawai sedangkan variabel yang paling dominan yaitu Faktor Kemampuan Kerja. This research aims to determine the effect of working environment, compensation and ability to simultaneously work on employees ' performance and to know the most dominant factor in the performance of employees in the village of the new Maros District Bodoa District, Maros. This study uses sample methods and data collection methods, data obtained by observation, interviews, questionnaires and documentation, samples in this study are based on NonProbability Sampling methods. NonProbability Sampling technique is selected with saturated Sampling (census) i.e. the method of sample withdrawal when all population members are made samples. The results of the test show that simultaneously the three variables have significant effect on the employee's performance while the most dominant variable is the job capability factor


1997 ◽  
Vol 87 (5) ◽  
pp. 445-453 ◽  
Author(s):  
J.D. Charlwood ◽  
T. Smith ◽  
P.F. Billingsley ◽  
W. Takken ◽  
E.O.K. Lyimo ◽  
...  

AbstractDelayed and immediate oocyst rates; parous rates and sporozoite rates were obtained in Anopheles gambiae Giles, A. arabiensis Patten and A. funestus Giles from two villages in the Kilombero Valley, southern Tanzania during the wet season of 1991. Collection methods included light trap, indoor resting collection and nets with holes cut in their side. Mosquito survival estimates from parous rates obtained from light trap collections, were compared with estimates from capture–recapture experiments and from that obtained during a population decline. Methods of estimating the proportion of feeds infectious to mosquitoes, K, were also compared. This proportion varied between villages and species and was highest in the village with the greatest proportion of A. gambiae. We propose that absolute estimates of K should be obtained by determining the immediate oocyst rate and measuring the parous rate using the same host seeking mosquitoes. This estimate was only available from one village and ranged from 1.9% for A. gambiae s.l. to 3.4% for A. funestus.


2021 ◽  
Author(s):  
Sisay Dugassa Lemma ◽  
Mathew Murphy ◽  
Sheleme Chibsa ◽  
Yehualashet Tadesse ◽  
Gedeon Yohannes ◽  
...  

Abstract Background Ethiopia has made great strides in malaria control over the last two decades. However, this progress has not been uniform and one concern has been reported high rates of malaria transmission in large agricultural development areas in western Ethiopia. Improved vector control is one way this transmission might be addressed, but little is known about malaria vectors in this part of the country. Methods To better understand the vector species involved in malaria transmission and their behaviour, human landing collections were conducted in Dangur woreda, Benishangul-Gumuz, between July and December 2017. This period encompasses the months with the highest rain and the peak mosquito population. Mosquitoes were identified to species and tested for the presence of Plasmodium sporozoites. Results The predominant species of the Anopheles collected was Anopheles arabiensis (1,733; i.e. 61.3% of the entire Anopheles), which was also the only species identified with sporozoites (Plasmodium falciparum and Plasmodium vivax). Anopheles arabiensis was collected as early in the evening as 18:00h-19:00h, and host-seeking continued until 5:00h-6:00h. Nearly equal numbers were collected indoors and outdoors. The calculated entomological inoculation rate for An. arabiensis for the study period was 1.41 infectious bites per month. More An. arabiensis were collected inside and outside worker’s shelters than in fields where workers were working at night. Conclusions Anopheles arabiensis is likely to be the primary vector of malaria in the agricultural development areas studied. High rates of human biting took place inside and outdoor near workers’ residential housing. Improved and targeted vector control in this area might considerably reduce malaria transmission.


2020 ◽  
Author(s):  
Sisay Dugassa Lemma ◽  
Mathew Murphy ◽  
Sheleme Chibsa ◽  
Yehualashet Tadesse ◽  
Gedeon Yohannes ◽  
...  

Abstract Background: Ethiopia has made great strides in malaria control. However, this progress has not been uniform and one concern has been reported high rates of malaria transmission in large agricultural development areas in western Ethiopia. Improved vector control is one way this transmission might be addressed, but little is known about malaria vectors in this part of the country. Methods: To better understand the vector species involved in malaria transmission and their behavior, human landing collections were conducted in Dangur woreda, Benishangul-Gumuz, between July and December 2017. Mosquitoes were identified to species and tested for presence of Plasmodium sporozoites. Results: The predominant species collected was Anopheles arabiensis (61.3% of all Anopheles), which was also the only species identified with sporozoites (P. falciparum and P. vivax). Anopheles arabiensis was collected as early in the evening as 18:00h-19:00h, and host-seeking continued until 5:00h-6:00h. Nearly equal numbers were collected indoors and outdoors. The calculated entomological inoculation rate for An. arabiensis for the study period was 1.41 infectious bites per month. More An. arabiensis were collected inside and outside worker’s shelters than in fields where workers were working at night. Conclusions: An. arabiensis is likely to be the primary vector of malaria in the agricultural development areas studied. High rates of human biting took place inside and outdoor near workers’ residential housing. Improved and targeted vector control in this area might considerably reduce malaria transmission.


2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Sisay Dugassa ◽  
Mathew Murphy ◽  
Sheleme Chibsa ◽  
Yehualashet Tadesse ◽  
Gedeon Yohannes ◽  
...  

Abstract Background Ethiopia has made great strides in malaria control over the last two decades. However, this progress has not been uniform and one concern has been reported high rates of malaria transmission in large agricultural development areas in western Ethiopia. Improved vector control is one way this transmission might be addressed, but little is known about malaria vectors in this part of the country. Methods To better understand the vector species involved in malaria transmission and their behaviour, human landing collections were conducted in Dangur woreda, Benishangul-Gumuz, between July and December 2017. This period encompasses the months with the highest rain and the peak mosquito population. Mosquitoes were identified to species and tested for the presence of Plasmodium sporozoites. Results The predominant species of the Anopheles collected was Anopheles arabiensis (1,733; i.e. 61.3 % of the entire Anopheles), which was also the only species identified with sporozoites (Plasmodium falciparum and Plasmodium vivax). Anopheles arabiensis was collected as early in the evening as 18:00 h-19:00 h, and host-seeking continued until 5:00 h-6:00 h. Nearly equal numbers were collected indoors and outdoors. The calculated entomological inoculation rate for An. arabiensis for the study period was 1.41 infectious bites per month. More An. arabiensis were collected inside and outside worker’s shelters than in fields where workers were working at night. Conclusions Anopheles arabiensis is likely to be the primary vector of malaria in the agricultural development areas studied. High rates of human biting took place inside and outdoor near workers’ residential housing. Improved and targeted vector control in this area might considerably reduce malaria transmission.


2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Ahmad Ubaidillah ◽  
Misbahul Khoir

The objectives of research include; first, to describe what local Islamic working ethos are as the basis for the resilience of songkok, whip and slap handicraft businesses in Serah Panceng Gresik Village. Second, to describe the resilience of the songkok, whip and slap handicraft business in the village of Serah Panceng Gresik. This study is a qualitative-descriptive study with the aim of understanding the phenomena experienced by the subject of research including behavior, perception, motivation, and action holistically by utilizing various scientific methods. Data collection methods include; Observation, In-depth Interview or Focus Group Discussion, Documentation. Data analysis techniques include: processing and preparing data for analysis, reading the entire data, analyzing in more detail by coding data, considering detailed instructions that can help the coding process, giving descriptions that will be presented in the report, interpreting and interpreting data. The results showed that in Serah Village local Islamic working ethos were preserved by the community, such as alms giving, reading dziba', reading tahlil, attending haul akbar, and reading sholawat together every Friday. Although in the tradition it does not involve songkok, whip, and slap directly, there is a good impact to support the resilience of songkok, but not whip, and slap production. Religious rituals by praying together asking Allah to facilitate and carry out business in production songkok, whip, and slap are an expression of gratitude for what God gave to the people of Serah Village. All economic activity done by Serah community is meant to get God’s willing. Keywords: Islamic Working Ethos, Handicraft Businesses


2020 ◽  
Vol 21 (2) ◽  
pp. 122-129
Author(s):  
Maria C K Nadjib ◽  
Alfetri N.P Lango ◽  
Paulus Un

The research, which was conducted in the village of Oepaha, Nekamese District, Kupang Regency, from June to July 2019, aims to identify marketing channels, capabilities and margins, and the share and benefits of celery marketing for farmers. The location of the research was determined using purposive sampling methods, considering that the location was the most important supplier area for celery in Kupang Regency. The population in this study was the farming community in Oepaha Village, Nekamese district, Kupang Regency,in the amount of 96 celery farmers. The sampling methods is carried out by simple random sampling according to the Slovenian formula, so that a sample of 49 celery farmers is obtained. Sampling for marketing institutions was determined by selecting the marketing institutions which directly involved in celery marketing using snowball sampling methods. In the end, respondents selected marketing institutes consisting of village collectors amounted to 4 people and retailers amounted to 4 people. The analysis of the data used in this study includes descriptive analysis, marketing margin analysis, farmer's share analysis and marketing profit analysis. The results showed that the marketing channel for celery carried out by farmers consisted of two channels, namely farmers directly to consumers and farmers to consumers through intermediaries, namely village collectors and retailers. Celery marketing functions that arise are the functions of sales, purchases, transport, standardization and financing and market information on the zero level channel,while sales, purchasing, transportation, storage, standardization, and financing, as well as market information, run on the second level channel. The marketing margin at zero level is Rp. 51.000,-, while the second level marketing channel is Rp. 17,000 at the collector and Rp. 34,000 at the retailer. The percentage of farmer’s share received by farmers is 25% at zero level and 25% at second level channel. The profit from celery marketing in the zero level marketing channel is Rp. 8.261 (farmers), the second level marketing channel is Rp. 16,688 (farmers), Rp. 15.267 (collectors' traders), Rp. 28,029 (retailers).


Publika ◽  
2021 ◽  
pp. 109-120
Author(s):  
Novi Ekri Nurwahyuni ◽  
Muhammad Farid Ma'ruf

Desa Wisata Jambu berdiri sejak tahun 2018  berlokasi di Kecamatan Kayen Kidul Kabupaten Kediri yang meraih penghargaan  nominasi kategori desa wisata terbaik 2018 dan 2019 pada Penghargaan Anugerah Desa. Pada fase awal pembangunan desa wisata menggunakan modal anggaran yang berasal dari Alokasi Dana Desa (ADD) dengan tujuan untuk meningkatkan pemberdayaan masyarakat desa. Penelitian ini merupakan penelitian deskriptif dengan menggunakan pendekatan kualitatif, serta metode pengumpulan data dilakukan secara langsung turun ke lapangan dengan wawancara, observasi, dan dokumentasi. Focus penelitian ini adalah meneliti mengenai permasalahan yang diangkat yaitu sumber daya manusia yang kurang responsive terhadap pengelolaan desa wisata. Desa wisata jambu memiliki 17 spot wisata diantaranya yaitu  Kebun Bibit Kediri, Wisata Edukasi Wiwit Padi, Permainan Outbond, Wisata Tubing Niagara, Wisata Edukasi Menangkap Lele, Agrowisata Petik Kelengkeng, Agrowisata Petik Alpukat, Agrowisata Buah Lokal, Wisata Edukasi Menanam Padi, Agrowisata Okulasi dan Tabulampat, Wisata Edukasi Angon Kerbau, Edukasi Pengolakah Yogurt, Edukasi Perah Kambing Ettawa, Edukasi Pengolahan Limbah Kayu “Ask Craft”, Wisata Sungai Sejuta Ikan, Edukasi Karawitan, Pasar Papringan. Pemerintah Desa Jambu gencar mengupayakan tumbuhnya pemberdayaan masyarakat dengan mengelola desa wisata. Tujuan pemberdayaan masyarakat melalui pengelolaan desa wisata Jambu yaitu untuk meningkatkan perekonomian masyarakat desa serta mewujudkan masyarakat desa yang mampu berdaya saing dan mandiri. Dalam proses pengelolaan desa wisata terdapat kendala atau hambatan yang terjadi yaitu tanggapan masyarakat yang tidak responsive terhadap konsep spot wisata yang sudah disepakati. Selain itu kurangnya akses sarana dan prasarana spot wisata juga menjadikan kondisi desa wisata yang tidak kondusif sehingga akan menurunkan daya tarik wisatawan. Kata kunci : Desa Wisata, Alokasi Dana Desa,  Pemberdayaan Masyarakat   Jambu Tourism Village was founded in 2018, located in Kayen Kidul District, Kediri Regency, which won the nomination award for the best tourism village category 2018 and 2019 at the Village Award. In the early phase of tourism village development using budget capital from the Village Fund Allocation (ADD) with the aim of increasing the empowerment of village communities. This research is a descriptive study using a qualitative approach, and data collection methods are carried out directly in the field with interviews, observation, and documentation. The focus of this research is to examine the problems raised, namely human resources who are not responsive to the management of tourist villages. Guava tourism village has 18 tourist spots including Kediri Seed Garden, Wiwit Paddy Educational Tour, Outbound Games, Niagara Tubing Tour, Catfish Catching Educational Tour, Longan Picking Agro, Avocado Picking Agro, Local Fruit Agro Tourism, Paddy Planting Educational Tour, Grafting Agro tourism and Tabulampat, Education Tour for Buffalo Angon, Education for Yogurt Processing, Education for Ettawa Goat Milk, Education for Making Rengginang, Education for Wood Waste Processing “Ask Craft”, One Million Fish River Tour, Education for Karawitan, Papringan Market. The Jambu Village Government is aggressively seeking the growth of community empowerment by managing a tourist village. The purpose of community empowerment through the management of the Jambu tourism village is to improve the economy of the village community and create a village community that is able to be competitive and independent. In the process of managing a tourism village, there are obstacles or obstacles that occur, namely the response of the community that is not responsive to the agreed tourist spot concept. In addition, the lack of access to tourist spot facilities and infrastructure also makes the condition of the tourist village not conducive so that it will reduce the attractiveness of tourists. Keywords: Tourism Village, Village Fund Allocation, Community Empowerment


2020 ◽  
Author(s):  
Triwibowo Ambar Garjito ◽  
Lulus Susanti ◽  
Mujiyono Mujiyono ◽  
Mega Tyas Prihatin ◽  
Dwi Susilo ◽  
...  

Abstract BackgroundSeveral methods exist to collect and assess the abundance of dengue vector mosquitoes, i.e. morning adult collection using an aspirator, pupal collection, various ovitraps, whole night collection using human landing methods, and larval collection. This diversity of methods might be a source of variability and lack of statistical significance when trying to correlate mosquito density and risk of dengue outbreak. There is also a lack of published data regarding the effectiveness of these methods MethodsA mosquito survey was conducted in 39 locations corresponding to 15 dengue endemic provinces in Indonesia. The larval surveys were performed by collecting at least a single Aedes larva from each container, and then reared up until hatching. Three adult mosquito sampling methods were also used, including morning resting collection, human landing collection, animal baited trap. All field samples were tested for dengue. Factor Analysis of Mixed Data (FAMD) was conducted to analyze the effectiveness of the collection methods against mosquito species and dengue incidence.ResultsA total of 44,675 mosquitoes were collected. The single larva method was the most efficient method. Out of a total of 89 dengue-positive pools, the most frequently encountered virus was DENV2, which made up half of the positive samples, followed by DENV3 and DENV1, respectively. FAMD showed that no correlation could be found between any methods and the presence of dengue virus in mosquitoes. Moreover, no correlation could be found between either any methods or the incidence.ConclusionsThere was no concistency in the efficacy of a given method and the incidence of dengue in the human population. There was no correlation between any of the parameters considered, i.e. method, incidence of dengue, location and presence of dengue virus in mosquitoes. This indicates that entomological factors are not reliable indicators.


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