scholarly journals Combination of circulating miR-145-5p/miR-191-5p as biomarker for breast cancer detection

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10494
Author(s):  
Yeldar Ashirbekov ◽  
Arman Abaildayev ◽  
Nazgul Omarbayeva ◽  
Dauren Botbayev ◽  
Ayaz Belkozhayev ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Detection ◽  
Roc Analysis ◽  
Diagnostic Value ◽  
Control Group ◽  
Endogenous Control ◽  
Women Patients ◽  
Diagnosis And Prognosis ◽  
Kazakh Population ◽  
Plasma Mirnas

Background Breast cancer (BC) is the most common cancer among women worldwide. At present, there is a need to search for new, accurate, reliable, minimally invasive and cheap biomarkers in addition to existing methods for the diagnosis and prognosis of BC. The main goal of this study was to test the diagnostic value of six circulating miRNAs in Kazakh women. Materials and methods TaqMan-based miRNA profiling was conducted using plasma specimens from 35 BC women patients and 33 healthy women samples (control group). Results The level of all seven miRNAs (including endogenous control) normalized by synthetic cel-miR-39 were significantly elevated in the group of BC patients. Normalization using miR-222-3p as endogenous control reduced differences in level of miRNAs between groups; as a result, only three miRNAs were significantly upregulated in the group of BC patients—miR-145-5p (P = 6.5e−12), miR-191-5p (P = 3.7e−10) and miR-21-5p (P = 0.0034). Moreover, ROC analysis showed that the use of miR-145-5p and miR-191-5p, both individually (AUC = 0.931 and 0.904, respectively) or in combination (AUC = 0.984), allows to accurately differentiate BC patients from healthy individuals. Conclusions Two plasma miRNAs—miR-145-5p and miR-191-5p—are potential biomarkers for diagnosis of BC in the Kazakh population. The findings need to be further substantiated using a more representative sample.

scholarly journals A Novel Paradigm Based on ST2 and Its Contribution towards a Multimarker Approach in the Diagnosis and Prognosis of Heart Failure: A Prospective Study during the Pandemic Storm

Life ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1080
Author(s):  
Radu-Stefan Miftode ◽  
Daniela Constantinescu ◽  
Corina Maria Cianga ◽  
Antoniu Octavian Petris ◽  
Amalia-Stefana Timpau ◽  
...  
Keyword(s):  
Heart Failure ◽  
Area Under The Curve ◽  
Serum Levels ◽  
Diagnostic Value ◽  
Control Group ◽  
Diagnosis And Prognosis ◽  
Test Kit ◽  
Positive Correlations ◽  
Lactate Levels ◽  
A Prospective Study

Background: Acute heart failure (HF) represents an increasingly common and challenging presentation in the emergency room, also inducing a great socio-economic burden. Extensive research was conducted toward finding an ideal biomarker of acute HF, both in terms of sensitivity and specificity, but today practicians’ interest has shifted towards a more realistic multimarker approach. Natriuretic peptides (NPs) currently represent the gold standard for diagnosing HF in routine clinical practice, but novel molecules, such as sST2, emerge as potentially useful biomarkers, providing additional diagnostic and prognostic value. Methods: We conducted a prospective, single-center study that included 120 patients with acute HF and 53 controls with chronic HF. Of these, 13 patients (eight with acute HF, five from the control group) associated the coronavirus-19 disease (COVID-19). The diagnosis of HF was confirmed by a complete clinical, biological and echocardiographic approach. Results: The serum levels of all studied biomarkers (sST2, NT-proBNP, cardiac troponin) were significantly higher in the group with acute HF. By area under the curve (AUC) analysis, we noticed that NT-proBNP (AUC: 0.976) still had the best diagnostic performance, closely followed by sST2 (AUC: 0.889). However, sST2 was a significantly better predictor of fatal events, showing positive correlations for both in-hospital and at 1-month mortality rates. Moreover, sST2 was also associated with other markers of poor prognosis, such as the use of inotropes or high lactate levels, but not with left ventricle ejection fraction, age, body mass index or mean arterial pressure. sST2 levels were higher in patients with a positive history of COVID-19 as compared with non-COVID-19 patients, but the differences were statistically significant only within the control group. Bivariate regression showed a positive and linear relationship between NT-proBNP and sST2 (r(120) = 0.20, p < 0.002). Conclusions: we consider that sST2 has certain qualities worth integrating in a future multimarker test kit alongside traditional biomarkers, as it provides similar diagnostic value as NT-proBNP, but is emerging as a more valuable prognostic factor, with a better predictive value of fatal events in patients with acute HF.

scholarly journals DIAGNOSTIC VALUE OF INTERLEUKIN-6 AND D-DIMER IN INFLAMMATORY BOWEL DISEASES

2021 ◽  
pp. 92-96
Author(s):  
А. P. Lutsyk ◽  
D. V. Shorikova
Keyword(s):  
Inflammatory Bowel Diseases ◽  
Interleukin 6 ◽  
Roc Analysis ◽  
Diagnostic Value ◽  
Control Group ◽  
Active Process ◽  
Montreal Classification ◽  
Bowel Diseases ◽  
Inflammatory Bowel ◽  
D Dimer

The article deals with the diagnostic value of changes in the content of interleukin-6 and D-dimer in patients with inflammatory bowel diseases (IBD) - Ulcerative Colitis (UC) and Crohn Disease (CD). Materials and methods: We have studied 34 patients with UC and 18 people with CD with continuously recurrent course. There are also analysed data of 15 patients with UC and 15 patients with CD in period of the remission. The control group included 30 healthy volunteers. Clinical, endoscopic examination, an immunoassay determination of the content of interleukin-6 (IL-6) and D-dimer, was carried out. CD activity was assessed using the Chron’s disease activity index. The localization of CD was established according to the Montreal classification. The clinical activity of the disease in patients with UC was determined using the classification according to Truelove-Witts. The value of the index consisted of the sum of points for each indicator, which allowed determining the activity of the inflammatory process. Statistical processing was performed using STATISTICA 10.0 (StatSoft. Inc., USA), at p<0.05, the discrepancies between the obtained data were considered statistically significant. Research results. According to the obtained data, mild activity was found in 7 patients with UC (20.6%) and 4 - with CD (22.3%), moderate severity - in 17 (50.0%) and 8 (44.4%) patients, severe - in 10 (29.4%) and 6 (33.3%) patients. In the remission stage, 15 patients with UC and 15 patients with CD were examined. According to the Montreal classification, lesions of the upper gastrointestinal tract (L4) were observed in 4 patients with CD (22.2%), terminal ileitis (L1) occurred in 10 patients (55.6%), 4 patients were diagnosed with ileocolitis (L3) - 22.2%. It has been established that patients with an active course of UC and CD had a higher level of proinflammatory markers and thrombosis markers (D-dimer and IL-6) compared with inactive course and control group (p<0.05). The D-dimer content in UC was 690±315 pg/l. It is confirmed the increase of the content of this marker in 2.55 times compared to control group and in 1.43 times than inactive course of the disease respectively. The level of IL-6 at active in course of UC was 3.36±1.78 pg/ml, increasing in 2.07 times against control and in 1.72 times compared with group of remission. In the active course of CD, the level of D-dimer reached 720±267 pg/ml, increasing in 2.67 times in relation to control and in 1.60 times with respect to patients under remission. The content of IL-6 in the active course of CD reached 4.07±2.17 pg/ml, increasing in 2.85 times in comparison to healthy individuals and in 2.83 times relatively patients with remission. While using the ROC-analysis, the most sensitive value as a “cut point” for the diagnosis of the active process in the course of IBD can be considered the content of the D-dimer in an amount of more than 650 pg/l. The calculated AUC in ROC-analysis for the IL-6 content was 73.7%±6.14% (61.7 - 85.7, p<0.001), indicating the prognostic value of the model. The values of the IL-6 content of more than 2.80 pg/ml are diagnostically significant to predict the active process in IBD.

Comments on the Logistic Function in ROC Analysis: Applications to Breast Cancer Detection

1982 ◽  
Vol 21 (01) ◽  
pp. 26-30 ◽  
Author(s):  
Joann Haberman ◽  
J. E. Goin
Keyword(s):  
Breast Cancer ◽  
Diagnostic Imaging ◽  
Cancer Detection ◽  
Task Analysis ◽  
Roc Analysis ◽  
Imaging Techniques ◽  
Logistic Function ◽  
Detection Task ◽  
Breast Cancer Detection ◽  
Statistical Methodology

ROC analysis is rapidly becoming the method of choice for determining the performance of diagnostic imaging techniques. This discussion is intended to provide scientists working in diagnostic imaging with a more in-depth view of the detection task analysis than is currently available in the statistical methodology literature. The logistic function is defined and its utility is illustrated by analyzing two breast cancer detection modalities.

Detection of COX-2 in liquid biopsy in patients with breast cancer

2020 ◽  
Vol 73 (12) ◽  
pp. 826-829
Author(s):  
Camilla Paulla de Souza ◽  
Beatriz Alves ◽  
Jaques Waisberg ◽  
Fernando Fonseca ◽  
Alipio de Oliveira Carmo ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Peripheral Blood ◽  
Tumour Size ◽  
Control Group ◽  
Extracellular Rna ◽  
Diagnosis And Prognosis ◽  
Cancer Aggressiveness ◽  
The Mean ◽  
Cox 2 ◽  
The Difference

AimsTo determine the expression of the cyclooxygenase-2 (COX-2) gene in patients with breast cancer attended at the Centro Universitário Saúde ABC/Faculdade de Medicina do ABC (CUS-ABC/FMABC) outpatient clinic. Breast cancer is the most common cancer in women worldwide. More than two million new cases are reported annually. An overexpression of COX-2 has been observed in many cancers. COX-2 is related to parameters of cancer aggressiveness, including tumour size, positive nodal state and lower survival, and to angiogenesis and resistance to apoptosis.Methods15 mL of peripheral blood was obtained from 34 patients and 21 healthy women. The extracellular RNA of QIAamp RNA was submitted to an RNA sequestration kit for RNA reverse transcriptase. Quantitative real-time PCR was performed using COX-2-specific oligonucleotides and the endogenous Glyceraldehyde-3-Phosphate Dehydrogenase gene.ResultsThe mean remission time was 53 years. The mean progression time was 33 months. The difference observed between the patient and control groups in median COX-2 expression (p<0.001) was significant.ConclusionsPatients with breast cancer showed a higher mean COX-2 expression in peripheral blood samples at diagnosis than the control group. Since this information could prove important in the diagnosis and prognosis of breast cancer, further research is required on larger patient samples.

The diagnostic value of ultrasonography as adjunct to mammography and clinical examination in breast cancer detection

2001 ◽  
Vol 37 ◽  
pp. S168
Author(s):  
K. Flobbe ◽  
A. Bosch ◽  
A. Kessels ◽  
G. Beets ◽  
M. von Meyenfeldt ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Clinical Examination ◽  
Cancer Detection ◽  
Diagnostic Value ◽  
Breast Cancer Detection

scholarly journals Diagnostic value of preoperative MRI dynamic contrast imaging (DCE-MRI) combined with diffusion-weighted imaging (DWI) in breast cancer detection

2018 ◽  
Vol 2 (6) ◽  
Author(s):  
Peng Jing ◽  
Qiang Zhang
Keyword(s):  
Breast Cancer ◽  
Cancer Detection ◽  
Diffusion Weighted Imaging ◽  
Diagnostic Value ◽  
Diagnostic Methods ◽  
Contrast Imaging ◽  
Diagnostic Efficacy ◽  
Dce Mri ◽  
Diffusion Weighted ◽  
The Difference

Abstract Objective: To investigate the diagnostic efficacy of preoperative MRI dynamic contrast imaging (DCE-MRI) combined with diffusion-weighted imaging (DWI) in detecting breast cancer.Methods: A retrospective study was performed to compare the results of DCE-MRI combined with DWI in 78 patients with breast cancer who were treated in our hospital between January 20 and December 2018.  Results: After diagnosis, the coincidence rate of diagnosis by DCE-MRI combined with DWI was significantly higher than ultrasound (91.0% versus 55.1%, respectively; P< 0.05). Among the two diagnostic methods, DCE-MRI combined with DWI imaging showed more obvious tumor signals, and the difference was statistically significant (P<0.05).Conclusion: Preoperative application of DCE-MRI combined with DWI can provide a more accurate and effective reference for surgical planning.

CIRCULATING MIR-145 AND MIR-21 IN BREAST CANCER IN THE KAZAKH POPULATION

Vestnik ◽  
2021 ◽  
pp. 61-68
Author(s):  
К.О. Шарипов ◽  
Е.Е. Аширбеков ◽  
А.О. Абайлдаев ◽  
Д.М. Ботбаев ◽  
А.М. Белкожаев ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Menopausal Status ◽  
Diagnostic Value ◽  
Clinicopathological Characteristics ◽  
Cancer Diagnostics ◽  
Age At Menarche ◽  
Cancer Type ◽  
Healthy Controls ◽  
Breast Cancer Patients ◽  
Diagnosis And Prognosis

Рак молочной железы является наиболее часто диагностирумым раком у женщин во всем мире. Несмотря на прогресс в скриниге, позволившим обнаруживать заболевание на более ранних стадиях и значительно уменьшить смертноть, имеется необходимость в поиске новых достоверных, малоинвазивных и дешевых биомаркеров для раннего обнаружения, диагностики и прогностики рака молочной железы. Целью данного исследования явилась проверка диагностичекой ценности двух циркулирующих микроРНК - miR-145-5p и miR-21-5p, рекомендованных в литературе в качестве маркеров рака молочной железы, на выборке казахских женщин (91 больных и 65 здоровых). Используя метод количественной ПЦР, мы обнаружили, что концентрации обеих микроРНК были достоверно повышены в плазме больных по сравнению со здоровыми контролями. Кроме того, была обнаружена связь с некоторыми клинико-патологическими характеристиками в группе пациентов: уровень miR-21-5p был ассоциирован с пролиферативной активностью опухоли, уровень miR-145-5p - с менопаузальным статусом, уровни обеих микроРНК - с возрастом менархе и употреблением алкоголя. ROC-анализ показал средний потенциал применимости miR-145-5p в диагностике РМЖ, и низкий потенциал для miR-21-5p. Breast cancer (BC) is the most commonly diagnosed cancer type in women around the world. Despite advances in screening that have made it possible to detect the disease at an earlier stage and significantly reduce mortality, there is a need to identify new reliable, minimally invasive and cheap biomarkers for the early detection, diagnosis and prognosis of breast cancer. The aim of this study was to test the diagnostic value of two circulating miRNAs: miR-145-5p and miR-21-5p, recommended in the literature as markers of breast cancer, in Kazakh women (91 patients and 65 healthy controls). Using the quantitative PCR technique, we found that both miR-145-5p and miR-21-5p levels were significantly elevated in the plasma of breast cancer patients in comparison to healthy controls. In addition, associations with some clinicopathological characteristics in the patient group were found: the level of miR-145-5p was associated with menopausal status; the level of miR-21-5p was associated with the proliferative activity of tumors; the levels of both miR-145-5p and miR-21-5p were associated with age at menarche and alcohol consumption. ROC-analysis showed an intermediate potential for usefulness in breast cancer diagnostics for miR-145-5p, and a low potential for miR-21-5p.

scholarly journals Diagnostic value of real-time shear wave elastography in children with chronic kidney disease

2020 ◽  
Author(s):  
Qin Liu ◽  
Wang Zhengrong
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Real Time ◽  
Shear Wave ◽  
Roc Analysis ◽  
Shear Wave Elastography ◽  
Diagnostic Value ◽  
Control Group ◽  
Case Group ◽  
Left And Right

Abstract Objective To investigate the diagnostic value of real-time shear wave elastography (SWE) in children with chronic kidney disease (CKD).Methods Children with CKD diagnosed by the ultrasound-guided biopsy between January 2018 and May 2019 were enrolled as the case group. Age- and sex- matched healthy children were selected as the control group. The Young’s Modulus (YM) of the renal cortex was measured by SWE after the traditional ultrasound examination. Variance analysis was performed to compare the values of YM between the two groups. Receiver operating characteristic curve (ROC) analysis was used to compare the values of YM, and explore the cut-offs of the YM.Results In the case group (n=60, 45% male, mean age of 9.2 years), the kidney YM modulus on the left side (16.8±4.8 Kpa vs. 8.3±2.1 Kpa) and the right side (16.0±4.7 Kpa vs. 8.3±2.4 Kpa) were both higher than the control group (all P values<0.001). With the progress of CKD, the YM value of the left and right kidneys gradually increased. ROC analysis showed that when the left and right kidney YM value was 11.7 KPa and 11.0Kpa, the diagnostic sensitivity and specificity were the highest (left: respectively 93.3% and 95.0%; right: respectively 93.3 % and 91.7%).Conclusion The increase of YM in CKD is related to the progression of renal dysfunction which may provide a new method for early diagnosis of CKD, dynamic monitoring of disease progression, and evaluation of curative effect and prognosis.

scholarly journals Diagnostic value of real-time shear wave elastography in children with chronic kidney disease

2020 ◽  
pp. 1-7
Author(s):  
Qin Liu ◽  
Zhengrong Wang
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Real Time ◽  
Shear Wave ◽  
Roc Analysis ◽  
Shear Wave Elastography ◽  
Diagnostic Value ◽  
Control Group ◽  
Case Group ◽  
Left And Right

OBJECTIVE: To evaluate the diagnostic value of real-time shear wave elastography (SWE) in children with chronic kidney disease (CKD). METHODS: Children with CKD diagnosed by the ultrasound-guided biopsy between January 2018 and May 2019 were enrolled as the case group. Age- and sex- matched healthy children were selected as the control group. The Young’s Modulus (YM) of the renal cortex was measured by SWE after the traditional ultrasound examination. Variance analysis was performed to compare the values of YM between the two groups. Receiver operating characteristic curve (ROC) analysis was used to compare the values of YM, and explore the cut-offs of the YM. RESULTS: In the case group (n = 60, 45% male, mean age of 9.2 years), the kidney YM modulus on the left side (16.8±4.8 kPa vs. 8.3±2.1 kPa) and the right side (16.0±4.7 kPa vs. 8.3±2.4 kPa) were both higher than the control group (all P values <  0.001). With the progress of CKD, the YM value of the left and right kidneys gradually increased. ROC analysis showed that when the left and right kidney YM value was 11.7 kPa and 11.0 kPa, the diagnostic sensitivity and specificity were the highest (left: respectively 93.3% and 95.0%; right: respectively 93.3 % and 91.7%). CONCLUSION: The increase of YM in CKD is related to the progression of renal dysfunction which may provide a new method for early diagnosis of CKD, dynamic monitoring of disease progression, and evaluation of curative effect and prognosis.

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