scholarly journals Expression of vitamin D 1α-hydroxylase in human gingival fibroblasts in vivo

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10279
Author(s):  
Kaining Liu ◽  
Bing Han ◽  
Jianxia Hou ◽  
Jianyun Zhang ◽  
Jing Su ◽  
...  
Keyword(s):  
Vitamin D ◽  
Stage Iv ◽  
Human Gingival Fibroblasts ◽  
Periodontal Pocket ◽  
Control Group ◽  
Gingival Fibroblasts ◽  
Connective Tissues ◽  
Periodontal Inflammation

Background Vitamin D 1α-hydroxylase CYP27B1 is the key factor in the vitamin D pathway. Previously, we analyzed the expression of CYP27B1 in human gingival fibroblasts in vitro. In the present study, we analyzed the gingival expression of CYP27B1 in vivo. Methods Forty-two patients with periodontitis Stage IV Grade C and 33 controls were recruited. All patients with periodontitis had unsalvageable teeth and part of the wall of the periodontal pocket was resected and obtained after tooth extraction. All controls needed crown-lengthening surgery, and samples of gingiva resected during surgery were also harvested. All the individuals’ gingivae were used for immunohistochemistry and immunofluorescence. In addition, gingivae from seventeen subjects of the diseased group and twelve subjects of the control group were analyzed by real-time PCR. Results Expression of CYP27B1 was detected both in gingival epithelia and in gingival connective tissues, and the expression in connective tissues colocalized with vimentin, indicating that CYP27B1 protein is expressed in gingival fibroblasts. The expression of CYP27B1 mRNA in gingival connective tissues and the CYP27B1 staining scores in gingival fibroblasts in the diseased group were significantly higher than those in the control group. Conclusions Expression of CYP27B1 in human gingival tissues was detected, not only in the fibroblasts of gingival connective tissues, but also in the gingival epithelial cells, and might be positively correlated with periodontal inflammation.

scholarly journals Effect of Sodium Lauryl Sulfate (SLS) on Primary Human Gingival Fibroblasts in an In Vitro Wound Healing Model

2019 ◽  
Vol 184 (Supplement_1) ◽  
pp. 97-101 ◽  
Author(s):  
Augustine H Chuang ◽  
Justin Bordlemay ◽  
Jeremy L Goodin ◽  
James C McPherson
Keyword(s):  
Wound Healing ◽  
Sodium Lauryl Sulfate ◽  
Potential Effect ◽  
Human Gingival Fibroblasts ◽  
In Vivo Studies ◽  
Control Group ◽  
Lauryl Sulfate ◽  
Gingival Fibroblasts

Abstract Objectives Sodium lauryl sulfate (SLS) is a surfactant used to decrease the surface tension of water. Most commercially available dentifrices contain 0.5–2.0% SLS. This study investigated the potential effect of SLS on oral wound healing using primary human gingival fibroblasts (HGFs). Methods HGFs cells were grown in12-well culture plates in DMEM medium. A 3 mm wound was created on confluent HGFs. The cells were challenged with 0 (the control group), 0.01, 0.02, 0.03, 0.04, or 0.05% SLS-containing media once daily for 2 minutes. The cells were stained on day 0, 2, 4, 6 and 8. The percent of wound fill area was measured. Results On day 2, 4, 6, and 8, the wound fill of the control group (0% SLS) was 15, 35, 67 and 98%, respectively; at 0.01% SLS, it was 10, 20, 65 and 84%; at 0.02%, it was 7, 10, 15 and 25%; at 0.03% SLS, it was only 5% and 8% on day 2 and 4. Conclusion Our results showed a dose- and time-dependent inhibition on HGFs wound fill by SLS; however, future in vivo studies are needed to validate if our in vitro findings using SLS-free dentifrices to avoid the potential delay of wound healing.

scholarly journals In vitro Analysis of Cytotoxicity of Temporary Resilient Relining Materials

2016 ◽  
Vol 17 (6) ◽  
pp. 457-462 ◽  
Author(s):  
Isleine P Caldas ◽  
Miriam Z Scelza ◽  
Marco A Gallito ◽  
Gutemberg Alves ◽  
Licínio Silva
Keyword(s):  
Cell Viability ◽  
Human Fibroblasts ◽  
Primary Cultures ◽  
Polystyrene Latex ◽  
Test Method ◽  
Human Gingival Fibroblasts ◽  
Control Group ◽  
Gingival Fibroblasts ◽  
Significant Difference

ABSTRACT Aims The aim of this study is to evaluate the in vitro response of human gingival fibroblasts in primary cultures to two materials for temporary relining of dentures: Temporary Soft (TDV, Brazil) and Trusoft (Bosworth, USA) for 24 hours, 7 and 30 days by using a multi-parametric analysis. Materials and methods Each material sample (TDV, TS, Polystyrene, Latex) was prepared and incubated in a culture medium for 1, 7, and 30 days at 37°C. Human gingival fibroblasts were exposed to the extracts and cell viability was evaluated by a multi-parametric assay, which allowed sequential analysis of mitochondrial activity (XTT), membrane integrity [neutral red (NR)], and cell density [crystal violet dye exclusion (CVDE)] in the same cells. Analysis of variance (ANOVA) was used to test the interactions of the three sources of variation (material, test method, and time) with the proportions of viable cells for each relining material. Results Both evaluated materials (TDV and TS) had low cytotoxic effects during 1, 7, and 30 days after manipulation of the material, as assessed by all three methods used. A statistical difference was found when comparing the negative control group (latex fragments) with the other groups, which showed high toxicity and low percentage of cell viability in all tests used. There was no significant difference among other materials (p > 0.05). Conclusion Low cytotoxicity levels were detected by representatives of the major groups of temporary prosthetic relining materials, as evaluated by multiple cellular viability parameters in human fibroblasts. Clinical significance There are various soft materials on the market for relining prostheses; however, the effects of these materials on tissues need to be clarified to avoid problems for patients. How to cite this article Caldas IP, Scelza MZ, Gallito MA, Alves G, Silva L. In vitro Analysis of Cytotoxicity of Temporary Resilient Relining Materials. J Contemp Dent Pract 2016;17(6):457-462.

scholarly journals Antibacterial Properties of Nano-Ag Coating on Healing Abutment: An In Vitro and Clinical Study

Antibiotics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 347 ◽  
Author(s):  
Tetsurou Odatsu ◽  
Shinichiro Kuroshima ◽  
Mika Sato ◽  
Kazuma Takase ◽  
Alireza Valanezhad ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Dental Plaque ◽  
Antibacterial Effect ◽  
Antibacterial Properties ◽  
Pure Titanium ◽  
Human Gingival Fibroblasts ◽  
Microwave Assisted Synthesis ◽  
Gingival Fibroblasts

Peri-implantitis is an inflammatory disease with a relevant focus on the long-term success of dental implants and implant-supported prostheses. The present study focuses on the antibacterial effect of the silver nanoparticle and investigated the suppression of dental plaque adhesion on implant abutment and/or superstructure by micro-wave assistant nanosilver coating in vivo and in vitro. Nanosilver coating on pure titanium was prepared by microwave-assisted synthesis, and characterized by scanning electron microscopy and energy-dispersive X-ray spectroscopy. In vitro studies were conducted to analyze biocompatibility using MTS assay and fluorescence microscopy with human gingival fibroblasts to evaluate antibacterial activity. During the in vivo study, nanosilver coating was applied to the healing abutments, and the prevention of plaque accumulation on nanosilver coating was confirmed by a split-mouth randomized clinical trial. The aggregation of nano-sized particles was found on the titanium surface with an antibacterial effect. The coating had no cytotoxic effect on human gingival fibroblasts. The result of the clinical trial showed that the coating suppressed the dental plaque adhesion on the healing abutments. Nanosilver coating is a promising material with antibacterial properties and can be used for implant abutments and prostheses for preventing peri-implantitis.

Comparative assessment of effectiveness of new drugs for targeted therapy of endometriosis by experimental model

GYNECOLOGY ◽  
2018 ◽  
Vol 20 (5) ◽  
pp. 46-51 ◽  
Author(s):  
M I Yarmoliskaya ◽  
M A Petrosyan ◽  
M S Florova ◽  
A S Molotkov ◽  
A S Denisova ◽  
...  
Keyword(s):  
Vitamin D ◽  
Targeted Therapy ◽  
New Drugs ◽  
Control Group ◽  
Oral Drugs ◽  
Significant Difference ◽  
Pathogenetic Therapy ◽  
Recurrent Nature

Introduction. The chronic, progressive, recurrent nature of the endometriosis results new avenues of targeted therapy for genital endometriosis with high therapeutic efficacy and minimal side effects must be explored. Nowadays, the standard of prolonged specific therapy for endometriosis is the dienogest 2 mg daily, which has already been proven to be effective in vitro, in vivo and in clinical practice. Purpose: to evaluate the effectiveness of new types of targeted pathogenetic therapy for endometriosis on the model of endometriosis in rats compared to dienogest and without treatment. Materials and methods. Endometriosis was induced on 69 Wistar rats by autotransplantation of uterine fragments onto the inner surface of the abdominal wall. After 14 days, the heterotopies had been measured by laparoscopy and then rats were randomized of into one of 6 experimental groups (dienogest, letrozole, cabergoline, metformin, vitamin D, melatonin) or a control group. All drugs were administered daily orally for three weeks, after which an autopsy and re-measuring of the size of endometrial implants were performed. Results. The most pronounced decrease in the size of endometrial implants was observed in the group of animals treated with dienogest (complete resorption - 48%, regression - 48%, without dynamics - 4%) and letrozole (complete resorption - 44%, regression - 56%) without the statistically significant difference between groups. In other groups, a significant decrease in the size of endometrial implants was demonstrated compared with the control, without a statistically significant difference between the groups. Findings. The presented study confirms the absence at the present time of oral drugs for the treatment of endometriosis, comparable in efficacy and safety with dienogest. Further research are needed to evaluate the different combinations of dopamine agonists, biguanides, vitamin D, melatonin as the supplement to the classic hormone-modulating therapy for endometriosis or as monotherapy in patients with contraindications to standard hormone therapy.

scholarly journals Identification of Interleukin-8-Reducing Lead Compounds Based on SAR Studies on Dihydrochalcone-Related Compounds in Human Gingival Fibroblasts (HGF-1 cells) In Vitro

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1382
Author(s):  
Katharina Schueller ◽  
Joachim Hans ◽  
Stefanie Pfeiffer ◽  
Jessica Walker ◽  
Jakob P. Ley ◽  
...  
Keyword(s):  
Cell Model ◽  
Periodontal Diseases ◽  
Oral Care ◽  
Interleukin 8 ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Periodontal Inflammation ◽  
Anti Inflammatory ◽  
Related Compounds

Background: In order to identify potential activities against periodontal diseases, eighteen dihydrochalcones and structurally related compounds were tested in an established biological in vitro cell model of periodontal inflammation using human gingival fibroblasts (HGF-1 cells). Methods: Subsequently to co-incubation of HGF-1 cells with a bacterial endotoxin (Porphyromonas gingivalis lipopolysaccharide, pgLPS) and each individual dihydrochalcone in a concentration range of 1 µM to 100 µM, gene expression of interleukin-8 (IL-8) was determined by qPCR and cellular interleukin-8 (IL-8) release by ELISA. Results: Structure–activity analysis based on the dihydrochalcone backbone and various substitution patterns at its aromatic ring revealed moieties 2′,4,4′,6′-tetrahydroxy 3-methoxydihydrochalcone (7) to be the most effective anti-inflammatory compound, reducing the pgLPS-induced IL-8 release concentration between 1 µM and 100 µM up to 94%. In general, a 2,4,6-trihydroxy substitution at the A-ring and concomitant vanilloyl (4-hydroxy-3-methoxy) pattern at the B-ring revealed to be preferable for IL-8 release inhibition. Furthermore, the introduction of an electronegative atom in the A,B-linker chain led to an increased anti-inflammatory activity, shown by the potency of 4-hydroxybenzoic acid N-vanillylamide (13). Conclusions: Our data may be feasible to be used for further lead structure designs for the development of potent anti-inflammatory additives in oral care products.

scholarly journals Influence of Nano, Micro and Macro Topography of Dental Implant Surfaces on Human Gingival Fibroblasts

2021 ◽  
Vol 22 (18) ◽  
pp. 9871
Author(s):  
Morena Petrini ◽  
Tania Vanessa Pierfelice ◽  
Emira D’Amico ◽  
Natalia Di Pietro ◽  
Assunta Pandolfi ◽  
...  
Keyword(s):  
Soft Tissues ◽  
Titanium Implant ◽  
Sem Analysis ◽  
Human Gingival Fibroblasts ◽  
Dependent Manner ◽  
Gingival Fibroblasts ◽  
Force Microscopy ◽  
Atomic Force

Current research on dental implants has mainly focused on the influence of surface roughness on the rate of osseointegration, while studies on the development of surfaces to also improve the interaction of peri-implant soft tissues are lacking. To this end, the first purpose of this study was to evaluate the response of human gingival fibroblasts (hGDFs) to titanium implant discs (Implacil De Bortoli, Brazil) having different micro and nano-topography: machined (Ti-M) versus sandblasted/double-etched (Ti-S). The secondary aim was to investigate the effect of the macrogeometry of the discs on cells: linear-like (Ti-L) versus wave-like (Ti-W) surfaces. The atomic force microscopy (AFM) and scanning electron microscopy (SEM) analysis showed that the Ti-S surfaces were characterized by a significantly higher micro and nano roughness and showed the 3D macrotopography of Ti-L and Ti-W surfaces. For in vitro analyses, the hGDFs were seeded into titanium discs and analyzed at 1, 3, and 5 days for adhesion and morphology (SEM) viability and proliferation (Cck-8 and MTT assays). The results showed that all tested surfaces were not cytotoxic for the hGDFs, rather the nano-micro and macro topography favored their proliferation in a time-dependent manner. Especially, at 3 and 5 days, the number of cells on Ti-L was higher than on other surfaces, including Ti-W surfaces. In conclusion, although further studies are needed, our in vitro data proved that the use of implant discs with Ti-S surfaces promotes the adhesion and proliferation of gingival fibroblasts, suggesting their use for in vivo applications.

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