Evaluation and comparison of hydroxyapatite nanoparticles’ cytotoxicity on L929 fibroblast and human peripheral blood mononuclear cells: an in vitro study

2012 ◽  
Vol 3 (2) ◽  
Author(s):  
H Shahoon ◽  
S Farhadi ◽  
R Hamedi ◽  
Z Yadegari ◽  
V Majd Al Hossein ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
In Vitro Study ◽  
Hydroxyapatite Nanoparticles ◽  
Peripheral Blood Mononuclear ◽  
L929 Fibroblast ◽  
Blood Mononuclear Cells

DNA damage and methylation induced by glyphosate in human peripheral blood mononuclear cells ( in vitro study)

2017 ◽  
Vol 105 ◽  
pp. 93-98 ◽  
Author(s):  
Marta Kwiatkowska ◽  
Edyta Reszka ◽  
Katarzyna Woźniak ◽  
Ewa Jabłońska ◽  
Jaromir Michałowicz ◽  
...  
Keyword(s):  
Dna Damage ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
In Vitro Study ◽  
Vitro Study ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals Oxidative Properties of Polystyrene Nanoparticles with Different Diameters in Human Peripheral Blood Mononuclear Cells (In Vitro Study)

2021 ◽  
Vol 22 (9) ◽  
pp. 4406
Author(s):  
Kinga Kik ◽  
Bożena Bukowska ◽  
Anita Krokosz ◽  
Paulina Sicińska
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
In Vitro Study ◽  
Polystyrene Nanoparticles ◽  
Oxidative Potential ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

With the ongoing commercialization, human exposure to plastic nanoparticles will dramatically increase, and evaluation of their potential toxicity is essential. There is an ongoing discussion on the human health effects induced by plastic particles. For this reason, in our work, we assessed the effect of polystyrene nanoparticles (PS-NPs) of various diameters (29, 44 and 72 nm) on selected parameters of oxidative stress and the viability of human peripheral blood mononuclear cells (PBMCs) in the in vitro system. Cells were incubated with PS-NPs for 24 h in the concentration range of 0.001 to 100 µg/mL and then labeled: formation of reactive oxygen species (ROS) (including hydroxyl radical), protein and lipid oxidation and cell viability. We showed that PS-NPs disturbed the redox balance in PBMCs. They increased ROS levels and induced lipid and protein oxidation, and, finally, the tested nanoparticles induced a decrease in PBMCs viability. The earliest changes in the PBMCs were observed in cells incubated with the smallest PS-NPs, at a concentration of 0.01 μg/mL. A comparison of the action of the studied nanoparticles showed that PS-NPs (29 nm) exhibited a stronger oxidative potential in PBMCs. We concluded that the toxicity and oxidative properties of the PS-NPs examined depended to significant degree on their diameter.

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