scholarly journals Tick bites (72 cases) between 2017 and 2019 at Ashikaga Red Cross Hospital, Tochigi Prefecture, Japan: Focus on 62 cases of Amblyomma testudinarium bites

2020 ◽  
Vol 71 (3) ◽  
pp. 219-223
Author(s):  
Mizuho Shimada ◽  
Hiroki Kawabata ◽  
Shuji Ando ◽  
Shichu Hou ◽  
Yumie Kobayashi ◽  
...  
Keyword(s):  
Red Cross ◽  
Tick Bites ◽  
Amblyomma Testudinarium

Annular Erythema Induced by Tick Bites due to 183 Larvae of Amblyomma testudinarium

2017 ◽  
Vol 79 (4) ◽  
pp. 349-352
Author(s):  
Hiroki SHIMIZU ◽  
Kotaro ITO ◽  
Monji KOGA ◽  
Shinichi IMAFUKU
Keyword(s):  
Tick Bites ◽  
Annular Erythema ◽  
Amblyomma Testudinarium

Ultrastructural observations of human monocytes infected with HIV-1

1991 ◽  
Vol 49 ◽  
pp. 108-109
Author(s):  
James K. Koehler ◽  
Steven G. Reed ◽  
Joao S. Silva
Keyword(s):  
Gradient Centrifugation ◽  
Virus Production ◽  
Human Monocyte ◽  
Red Cross ◽  
Human Monocytes ◽  
Blood Monocytes ◽  
Hiv Replication ◽  
Electron Microscopic ◽  
Ultrastructural Studies ◽  
Hiv 1

As part of a larger study involving the co-infection of human monocyte cultures with HIV and protozoan parasites, electron microscopic observations were made on the course of HIV replication and infection in these cells. Although several ultrastructural studies of the cytopathology associated with HIV infection have appeared, few studies have shown the details of virus production in “normal,” human monocytes/macrophages, one of the natural targets of the virus, and suspected of being a locus of quiescent virus during its long latent period. In this report, we detail some of the interactions of developing virons with the membranes and organelles of the monocyte host.Peripheral blood monocytes were prepared from buffy coats (Portland Red Cross) by Percoll gradient centrifugation, followed by adherence to cover slips. 90-95% pure monocytes were cultured in RPMI with 5% non-activated human AB serum for four days and infected with 100 TCID50/ml of HIV-1 for four hours, washed and incubated in fresh medium for 14 days.

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