PERFORMANCE OF THE TUBERCULIN SKIN TEST IN MYCOBACTERIUM BOVIS–EXPOSED AND –UNEXPOSED AFRICAN LIONS (PANTHERA LEO)

2019 ◽  
Vol 55 (3) ◽  
pp. 537 ◽  
Author(s):  
Ignatius M. Viljoen ◽  
Tashnica Taime Sylvester ◽  
Sven D. C. Parsons ◽  
Robert P. Millar ◽  
Paul D. van Helden ◽  
...  
Keyword(s):  
Tuberculin Skin Test ◽  
Skin Test ◽  
Mycobacterium Bovis ◽  
Panthera Leo ◽  
African Lions

scholarly journals Comparison of Two Commercially Available Gamma Interferon Blood Tests for Immunodiagnosis of Tuberculosis

2007 ◽  
Vol 15 (1) ◽  
pp. 168-171 ◽  
Author(s):  
Jose Domínguez ◽  
Juan Ruiz-Manzano ◽  
Malú De Souza-Galvão ◽  
Irene Latorre ◽  
Celia Milà ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Tuberculin Skin Test ◽  
Skin Test ◽  
Mycobacterium Bovis ◽  
Latent Infection ◽  
Positive Tuberculin Skin Test ◽  
Gamma Interferon ◽  
Bcg Vaccination ◽  
Blood Tests

ABSTRACT We evaluated the T-SPOT.TB and Quantiferon-TB Gold In tube (QFN-G-IT) tests for diagnosing Mycobacterium tuberculosis infection. T-SPOT.TB was more sensitive than QFN-G-IT in diagnosing both active and latent infection. Both gamma interferon tests were unaffected by prior Mycobacterium bovis BCG vaccination. Among children who were not BCG vaccinated but had a positive tuberculin skin test, QFN-G-IT was negative in 53.3% of cases, and T-SPOT.TB was negative in 50% of cases.

scholarly journals Comparison of Mantoux and QuantiFERON TB Gold Tests for Diagnosis of Latent Tuberculosis Infection in Army Personnel

2007 ◽  
Vol 14 (4) ◽  
pp. 477-480 ◽  
Author(s):  
Willeke P. J. Franken ◽  
Joost F. Timmermans ◽  
Corine Prins ◽  
Evert-Jan H. J. Slootman ◽  
Johan Dreverman ◽  
...  
Keyword(s):  
Tuberculin Skin Test ◽  
Skin Test ◽  
Mycobacterium Bovis ◽  
Military Personnel ◽  
Latent Tuberculosis Infection ◽  
Nontuberculous Mycobacteria ◽  
Latent Tuberculosis ◽  
Tuberculosis Infection ◽  
Army Personnel

ABSTRACT The tuberculin skin test (TST) was compared with QuantiFERON-TB Gold in-tube (QFT-GIT) test for the diagnosis of tuberculosis in non-Mycobacterium bovis BCG-vaccinated military personnel. Among subjects positive by TST, 44.4% of recruits were positive by QFT-GIT compared with 11.5% subjects tested after missions abroad, suggesting that most TST conversions in the latter group were caused by nontuberculous mycobacteria.

Intradermal tuberculin testing of wild African lions (Panthera leo) naturally exposed to infection with Mycobacterium bovis

2010 ◽  
Vol 144 (3-4) ◽  
pp. 384-391 ◽  
Author(s):  
D.F. Keet ◽  
A.L. Michel ◽  
R.G. Bengis ◽  
P. Becker ◽  
D.S. van Dyk ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Panthera Leo ◽  
Tuberculin Testing ◽  
African Lions

scholarly journals Temporal dynamics of intradermal cytokine response to tuberculin in Mycobacterium bovis BCG-vaccinated cattle using sampling microneedles

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sabine Steinbach ◽  
Sasan Jalili-Firoozinezhad ◽  
Sreenidhi Srinivasan ◽  
Mariane B. Melo ◽  
Sonya Middleton ◽  
...  
Keyword(s):  
Tuberculin Skin Test ◽  
Skin Test ◽  
Mycobacterium Bovis ◽  
Temporal Dynamics ◽  
Skin Testing ◽  
Tuberculin Skin Testing ◽  
Cytokines And Chemokines ◽  
Skin Response ◽  
Novel Biomarkers

AbstractBovine tuberculosis (bTB) is a disease of livestock with severe and worldwide economic, animal welfare and zoonotic consequences. Application of test-and-slaughter-based control polices reliant on tuberculin skin testing has been the mainstay of bTB control in cattle. However, little is known about the temporal development of the bovine tuberculin skin test response at the dermal sites of antigen injection. To fill this knowledge gap, we applied minimally-invasive sampling microneedles (SMNs) for intradermal sampling of interstitial fluid at the tuberculin skin test sites in Mycobacterium bovis BCG-vaccinated calves and determined the temporal dynamics of a panel of 15 cytokines and chemokines in situ and in the peripheral blood. The results reveal an orchestrated and coordinated cytokine and local chemokine response, identified IL-1RA as a potential soluble biomarker of a positive tuberculin skin response, and confirmed the utility of IFN-γ and IP-10 for bTB detection in blood-based assays. Together, the results highlight the utility of SMNs to identify novel biomarkers and provide mechanistic insights on the intradermal cytokine and chemokine responses associated with the tuberculin skin test in BCG-sensitized cattle.

Optimisation of the tuberculin skin test for detection of Mycobacterium bovis in African buffaloes (Syncerus caffer)

2021 ◽  
pp. 105254
Author(s):  
Katrin Smith ◽  
Netanya Bernitz ◽  
David Cooper ◽  
Tanya J. Kerr ◽  
Candice R. de Waal ◽  
...  
Keyword(s):  
Tuberculin Skin Test ◽  
Skin Test ◽  
Mycobacterium Bovis ◽  
Syncerus Caffer

scholarly journals Cellular immune response of Curraleiro Pé-duro and Nellore calves following Mycobacterium bovis-BCG vaccination

2013 ◽  
Vol 33 (12) ◽  
pp. 1403-1408
Author(s):  
Mayara Fernanda Maggioli ◽  
Joyce Rodrigues Lobo ◽  
Maria Clorinda Soares Fioravanti ◽  
André Kipnis ◽  
Ana Paula Junqueira-Kipnis
Keyword(s):  
Immune Response ◽  
Tuberculin Skin Test ◽  
Skin Test ◽  
Mycobacterium Bovis ◽  
Blood Levels ◽  
Cd4 Cells ◽  
Test Reaction ◽  
Positive Role ◽  
Bcg Vaccination ◽  
Ifn Γ

The present study aimed to assess the CD4, CD8 and γδ blood levels for Curraleiro Pé-duro, as well as the specific IFN-γ response after BCG vaccination using flow cytometry. The specific immune response against BCG was also evaluated by tuberculin skin test, performed before and 45 days after the vaccination. For comparison purposes, the same parameters were investigated on Nellore calves, an exotic bovine with resistance previously demonstrated. Naturally, Curraleiro Pé-duro animals had greater levels of CD4, CD8 and γδ lymphocytes (p<0.05). In response to vaccine, Curraleiro Pé-duro showed greater ability to respond specifically to BCG, generating resistance profile (Th1), evidenced by greater number of antigen specific CD4+ cells producing IFN-γ (p<0.05) and also higher tuberculin skin test reaction (p<0.05). Additionally, vaccinated Curraleiro Pé-duro calves had higher CD4 cells numbers than both Nellore control (p<0.05) and vaccinated groups (p<0.05). Curraleiro Pé-duro calves' higher basal lymphocytes blood level and stronger response in both IFN-γ and tuberculin skin test parameters probably play a positive role on protection/resistance to Mycobacterium bovis.

scholarly journals Tuberculosis in water buffalo (Bubalus bubalis) in the Baixo Araguari Region, Amapá, Brazil

2016 ◽  
Vol 37 (2) ◽  
pp. 885
Author(s):  
Silvia Minharro ◽  
Cristiane De Morais Alves ◽  
Pedro Moacyr Pinto Coelho Mota ◽  
Elaine Maria Seles Dorneles ◽  
Andréa Padilha de Alencar ◽  
...  
Keyword(s):  
Tuberculin Skin Test ◽  
Skin Test ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Water Buffalo ◽  
Bubalus Bubalis ◽  
Economic Importance ◽  
Brazilian Amazonia ◽  
Great Economic Importance ◽  
River Region

Water buffalo are of great economic importance in Brazilian Amazonia, which has the largest herd in Brazil. Bovine tuberculosis caused by Mycobacterium bovis is a zoonotic disease that results in severe losses to water buffalo production. Although the disease has already been described in the country, data on the occurrence and distribution of bovine tuberculosis in water buffalo in Brazil is very scarce. The aim of this study was to determine the frequency of bovine tuberculosis in water buffalo in the Baixo Araguari River region of Amapá, Brazil. Thirty herds, randomly selected from the 41 herds of water buffalo in the region, were sampled. From those herds, 212 randomly selected water buffalo were subjected to the comparative tuberculin skin test. The proportion of Baixo Araguari River region herds that were positive for bovine tuberculosis was 50.0% (95% CI 31.3% to 68.7%) and the proportion of animals that were positive was estimated to be 14.8% (95% CI 7.8% to 21.9%). Our results show that bovine tuberculosis is spread widely among water buffalo in the Baixo Araguari River region, which suggests that measures to control the disease should be undertaken in the region.

Antemortem Diagnosis of Mycobacterium bovis Infection in Free-ranging African Lions (Panthera leo) and Implications for Transmission

2015 ◽  
Vol 51 (2) ◽  
pp. 493-497 ◽  
Author(s):  
Michele Miller ◽  
Peter Buss ◽  
Jennifer Hofmeyr ◽  
Francisco Olea-Popelka ◽  
Sven Parsons ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Panthera Leo ◽  
Free Ranging ◽  
African Lions
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