Serologic Survey of Brucella canis and Leptospira spp. in Free-Ranging Wild and Domestic Canids from Tierra del Fuego, Chile

2019 ◽  
Vol 55 (3) ◽  
pp. 713
Author(s):  
Sebastián Moya ◽  
Sofía Oettinger ◽  
Consuelo Borie ◽  
Roberto Flores ◽  
Pedro Abalos ◽  
...  
Keyword(s):  
Tierra Del Fuego ◽  
Brucella Canis ◽  
Leptospira Spp ◽  
Serologic Survey ◽  
Free Ranging

scholarly journals Antibodies produced by dogs successfully challenged with live Leptospira spp. did not cross-react to Brucella antigen in a commercial rapid slide agglutination test that detects antibodies to Brucella canis

2018 ◽  
Vol 31 (1) ◽  
pp. 83-85
Author(s):  
Matthew R. Krecic
Keyword(s):  
Cross Reactivity ◽  
Agglutination Test ◽  
Slide Agglutination ◽  
Serologic Tests ◽  
Experimental Infections ◽  
Negative Results ◽  
Brucella Canis ◽  
Slide Agglutination Test ◽  
Leptospira Spp ◽  
Canine Brucellosis

Brucella canis is a cause of canine infertility and abortion. Veterinarians and veterinary laboratorians screen for antibodies to B. canis with serologic tests including a rapid slide agglutination test (RSAT; D-Tec CB, Zoetis, San Diego, CA). False-positive results are possible because of cross-reactivity to antibodies to some gram-negative bacteria. Cross-reactivity has been reported between antibodies of Brucella abortus and Leptospira spp. with serologic tests for bovine brucellosis; however, this has not been documented with serologic tests for canine brucellosis, to the author’s knowledge. The RSAT was evaluated with the sera from dogs experimentally challenged with 1 of 4 serovars of Leptospira spp.: L. kirschneri serovar Grippotyphosa, or L. interrogans serovars Canicola, Icterohaemorrhagiae, or Pomona. Experimental infections were confirmed through results of microscopic agglutination testing and/or lateral flow immunochromatography testing. The sera of 32 dogs collected at day 0 and days 7, 10, and 14 yielded negative results with the RSAT. Antibodies produced through experimental infections to these 4 serovars of Leptospira spp. did not cross-react with Brucella antigen with the RSAT; therefore, cross-reactivity of anti-leptospiral antibodies may not be of concern for B. canis rapid slide agglutination testing of dogs.

scholarly journals Seroprevalence for brucellosis and leptospirosis in dogs from Belém and Castanhal, State of Pará, Brazil

2015 ◽  
Vol 45 (3) ◽  
pp. 265-270 ◽  
Author(s):  
Giselle Souza da PAZ ◽  
Katarine de Souza ROCHA ◽  
Michele de Souza LIMA ◽  
Ediene Moura JORGE ◽  
José Carlos Figueiredo PANTOJA ◽  
...  
Keyword(s):  
Brucella Abortus ◽  
Bacterial Infections ◽  
Agar Gel ◽  
Source Of Infection ◽  
Brucella Canis ◽  
Leptospira Spp ◽  
The City

Brucellosis and leptospirosis are widely spread bacterial infections and dogs are the most important source of infection and reservoir for diseases. Dogs can disseminate the agents in the environment and transmit them to humans and/or other animals. The objective of this study was assess the occurrence of reactive to antibodies anti-Leptospira spp., Brucella canis and B. abortus in Belém and Castanhal, State of Pará, Amazon, Brazil. A total of 156 samples were randomly collected in the city of Belém and 158 samples in Castanhal. The anti-B. canis antibodies research was performed by Agar Gel Immunodiffusion (AGID) with and without 2-mercaptoethanol serum treatment (AGID-2ME). To assess the anti-B. abortus antibodies, the technique of Fast Seroagglutination with buffered acidified plate antigen (BAPAT) was used. For anti-Leptospira spp. antibodies research, the Microscopic Agglutination Technique (MAT) was used. No animal reacted to Brucella abortus and one animal was reactive to B. canis at the AGID, but it was negative to the AGID-2ME test. Seventeen percent of dogs (47/274) presented anti-Leptospira spp. antibodies, with prevalence of serovar Canicola. The dogs from Belém and Castanhal are not source of infection for B. abortus and B. canis, however, they are reservoirs for different serovars of Leptospira spp.

scholarly journals SEROLOGIC SURVEY FOR ANTIBODIES AGAINST MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS IN FREE-RANGING CERVIDS FROM NORWAY

2004 ◽  
Vol 40 (1) ◽  
pp. 32-41 ◽  
Author(s):  
Morten Tryland ◽  
Ingrid Olsen ◽  
Turid Vikøren ◽  
Kjell Handeland ◽  
Jon M. Arnemo ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Mycobacterium Avium Subsp ◽  
Serologic Survey ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Free Ranging

Antibodies to Toxoplasma gondii, Leishmania spp., and Leptospira spp. in Free-Ranging Six-Banded Armadillos (Euphractus sexcinctus) from Northeastern Brazil

2020 ◽  
Vol 56 (2) ◽  
pp. 486
Author(s):  
Werona de Oliveira Barbosa ◽  
Thiago Galvão Coelho ◽  
Talita Otaviano da Costa ◽  
Laís Moraes Paiz ◽  
Felipe Fornazari ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Northeastern Brazil ◽  
Leptospira Spp ◽  
Leishmania Spp ◽  
Euphractus Sexcinctus ◽  
Free Ranging

scholarly journals PREVALENCE OF ANTI-LEPTOSPIRA SPP. ANTIBODIES IN SWINE SLAUGHTERED IN THE PUBLIC SLAUGHTERHOUSE OF PATOS CITY, PARAÍBA STATE, NORTHEAST REGION OF BRAZIL

2008 ◽  
Vol 75 (4) ◽  
pp. 517-520
Author(s):  
S.S. Azevedo ◽  
R.M. Oliveira ◽  
C.J. Alves ◽  
D.M. Assis ◽  
S.F. Aquino ◽  
...  
Keyword(s):  
Statistical Difference ◽  
Agglutination Test ◽  
Microscopic Agglutination Test ◽  
Northeast Region ◽  
The Public ◽  
Serologic Diagnosis ◽  
Leptospira Spp ◽  
Serologic Survey

ABSTRACT A serologic survey was conducted among 131 swine slaughtered in the public slaughterhouse of Patos city, Northeast region of Brazil, to determine the prevalence of anti-Leptospira spp. agglutinins. For serologic diagnosis of leptospirosis, the microscopic agglutination test (MAT) was carried out using live cultures of 22 pathogenic and two saprophytic Leptospira spp. serovars. The most frequent serovar was found crossing the results of frequency and titer of agglutinins, and sera presenting equal titers for two or more serovars were not considered for this analysis. Of the 131 swine analyzed, 44 were seropositive for at least one Leptospira spp. serovar, resulting in a seroprevalence of 33.6% (95% CI = 25.5% 42.4%). The most frequent serovar was Pomona, with 38 (29.0%; 95% CI = 21.4% 37.6%) reactant sera. Other reactant serovars and respective prevalence were: Pyrogenes (2.3%; 95% CI = 0.5% 6.5%), Canicola (1.5%; 95% CI = 0.2% 5.4%) and Shermani (0.8%; 95% CI = 0.02% 4.2%). There was statistical difference in seroprevalence to serovar Pomona compared with others reactant serovars (P < 0.0001).

scholarly journals Guanaco’s diet and forage preferences in Nothofagus forest environments of Tierra del Fuego, Argentina

2017 ◽  
Vol 26 (1) ◽  
pp. e004 ◽  
Author(s):  
Claudia-Pamela Quinteros ◽  
José-Omar Bava ◽  
Miriam-Edith Gobbi ◽  
Guillermo-Emilio Defossé
Keyword(s):  
Tierra Del Fuego ◽  
Forest Understory ◽  
Grass Species ◽  
South American ◽  
Nothofagus Pumilio ◽  
Lama Guanicoe ◽  
Nothofagus Forests ◽  
Fresh Feces ◽  
Free Ranging ◽  
Composite Samples

Aim of study: Guanaco (Lama guanicoe Müller), is a South American native ungulate widely distributed in Patagonia, which in the island of Tierra del Fuego (TF), extends its habitat into Nothofagus spp. forests. Within these forests, guanacos consume lenga (Nothofagus pumilio) leaves and twigs, and other understory species. The aim of this work was to determine: 1) the spring and summer diet of free ranging guanacos, and 2) which plants, grown in the forest understory, guanacos do prefer, or avoid, in these seasons of great forage abundance.Area of study: Tierra del Fuego (Argentina), on three representative areas which combined Nothofagus forests and adjacent meadows (vegas).Material and Methods: Guanacos’ diet was determined by comparing epidermal and non-epidermal plant fragments with micro-histological analyses of feces. The analysis was made from composite samples of fresh feces, collected at the seasons of maximum forage productivity (spring and summer).Main results: During spring, 48% of guanacos’ diet was composed of lenga leaves, 30% of grass-like species, 15% of grasses, and less than 7% of herbs, shrubs, and lichens. In summer, 40% of the diet was composed of grasses, 30% of lenga leaves, 25% of grass-like species and the rest corresponded to herbs, shrubs, and lichens. Within the forest understory, guanaco selected lenga leaves and twigs, grass species were consumed according to their availability (or sometimes rejected), while other herbs were not consumed at all.Research highlights: Guanacos’ consumption preference for lenga, even considering the high availability of other forages, could adversely affect forest regeneration.

SEROLOGIC SURVEY OF LEPTOSPIRA SPP. IN CAPTIVE ANIMALS FROM VERACRUZ, MEXICO

2020 ◽  
Vol 51 (1) ◽  
pp. 222
Author(s):  
Carlos David Pérez-Brígido ◽  
Dora Romero-Salas ◽  
Sokani Sánchez-Montes ◽  
Javier Hermida-Lagunes ◽  
José Luis Ochoa ◽  
...  
Keyword(s):  
Leptospira Spp ◽  
Serologic Survey
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