CHARACTERIZATION OF PORCINE PARVOVIRUS TYPE 3 AND PORCINE CIRCOVIRUS TYPE 2 IN WILD BOARS (SUS SCROFA) IN SLOVAKIA

2015 ◽  
Vol 51 (3) ◽  
pp. 703-711 ◽  
Author(s):  
Ivan Sliz ◽  
Michaela Vlasakova ◽  
Anna Jackova ◽  
Stefan Vilcek
Keyword(s):  
Sus Scrofa ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Porcine Parvovirus ◽  
Wild Boars ◽  
Type 3

Genetic characterization of porcine circovirus type 2 in captive wild boars in southern Brazil

2017 ◽  
Vol 49 (5) ◽  
pp. 1071-1075 ◽  
Author(s):  
José Paulo Hiroji Sato ◽  
Danielle Gava ◽  
Rejane Schaefer ◽  
Maurício Egídio Cantão ◽  
Janice Reis Ciacci-Zanella ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Southern Brazil ◽  
Wild Boars

scholarly journals Immunohistochemical and ultra-structural detection ofPneumocystisin wild boars (Sus scrofa) co-infected with porcine circovirus type 2 (PCV2) in Southern Brazil

2011 ◽  
Vol 49 (2) ◽  
pp. 172-175 ◽  
Author(s):  
Mauro Riegert Borba ◽  
Edna Maria Cavallini Sanches ◽  
André Mendes Ribeiro Corrêa ◽  
Andréia Spanamberg ◽  
Juliano de Souza Leal ◽  
...  
Keyword(s):  
Sus Scrofa ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Southern Brazil ◽  
Wild Boars

scholarly journals Genetic characterization of porcine circovirus type 2 in piglets from PMWS-affected and -negative farms in Thailand

2011 ◽  
Vol 8 (1) ◽  
pp. 88 ◽  
Author(s):  
Tippawan Jantafong ◽  
Alongkot Boonsoongnern ◽  
Pariwat Poolperm ◽  
Kitcha Urairong ◽  
Chalermpol Lekcharoensuk ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus

Characterization of Immune Response of Young Pigs to Porcine Circovirus Type 2 Infection

2000 ◽  
Vol 13 (2) ◽  
pp. 143-153 ◽  
Author(s):  
ROMAN M. POGRANICHNYY ◽  
KYOUNG-JIN YOON ◽  
PERRY A. HARMS ◽  
SABRINA L. SWENSON ◽  
JEFFREY J. ZIMMERMAN ◽  
...  
Keyword(s):  
Immune Response ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Young Pigs

scholarly journals Simultaneous detection of porcine pseudorabies virus, porcine parvovirus and porcine circovirus type 2 by multiplex real-time PCR and amplicon melting curve analysis using SYBR Green I

2018 ◽  
Vol 63 (No. 8) ◽  
pp. 358-366
Author(s):  
LL Zheng ◽  
XH Jin ◽  
FS Wei ◽  
CQ Wang ◽  
HY Chen ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pseudorabies Virus ◽  
Simultaneous Detection ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Porcine Parvovirus ◽  
Porcine Pseudorabies Virus

Porcine parvovirus, porcine pseudorabies virus and porcine circovirus type 2 can cause reproductive failure in pigs, and swine are often simultaneously infected by combinations of the three viruses. We here report the development of a SYBR Green I-based multiplex real time PCR assay for simultaneous detection of porcine parvovirus, porcine pseudorabies virus and porcine circovirus type 2. Three pairs of specific primers were designed for the porcine parvovirus-VP2, porcine pseudorabies virus-gH and porcine circovirus type 2-ORF2 genes. Viral genomes were identified based on their distinctive melting temperatures in singleplex PCR reactions. The melting temperature was 74.5 °C for the 313 bp amplicon of porcine parvovirus-VP2 gene, 87.5 °C for the 355 bp amplicon of porcine pseudorabies virus-gH gene and 80.5 °C for the 171 bp amplicon of the porcine circovirus type 2-ORF2 gene, respectively. The detection limit of the method ranged from 0.01–0.03 TCID<sub>50</sub>/ml for the three viruses. In addition, porcine parvovirus, porcine pseudorabies virus and porcine circovirus type 2 viral loads were measured in 100 field samples, and the result showed that the concordance between real-time PCR and conventional PCR was 60.42%. The sensitivity and specificity of real-time PCR were 100% and 100%, while those of conventional PCR were 40.83% and 72.22%, respectively.

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