scholarly journals Insensitivity of PI3K/Akt/GSK3 signaling in peripheral blood mononuclear cells of age-related macular degeneration patients

2016 ◽  
Keyword(s):  
Macular Degeneration ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Age Related Macular Degeneration ◽  
Peripheral Blood Mononuclear ◽  
Age Related ◽  
Blood Mononuclear Cells

scholarly journals The Effect of Sulforaphane on Glyoxalase I Expression and Activity in Peripheral Blood Mononuclear Cells

Nutrients ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1773 ◽  
Author(s):  
Michela Alfarano ◽  
Donato Pastore ◽  
Vincenzo Fogliano ◽  
Casper Schalkwijk ◽  
Teresa Oliviero
Keyword(s):  
Gene Expression ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Limit Of Detection ◽  
Peripheral Blood Mononuclear ◽  
Potential Health ◽  
Age Related ◽  
Blood Mononuclear Cells ◽  
Expression Activity

Studies demonstrate that the potential health-beneficial effect of sulforaphane (SR), a compound formed in broccoli, is the result of a number of mechanisms including upregulation of phase two detoxification enzymes. Recent studies suggest that SR increases expression/activity of glyoxalase 1 (Glo1), an enzyme involved in the degradation of methylglyoxal, is major precursor of advanced glycation end products. Those compounds are associated with diabetes complications and other age-related diseases. In this study, the effect of SR on the expression/activity of Glo1 in peripheral blood mononuclear cells (PBMCs) from 8 healthy volunteers was investigated. PBMCs were isolated and incubated with SR (2.5 μM-concentration achievable by consuming a broccoli portion) for 24 h and 48 h. Glo1 activity/expression, reduced glutathione (GSH), and glutathione-S-transferase gene expression were measured. Glo1 activity was not affected while after 48 h a slight but significant increase of its gene expression (1.03-fold) was observed. GSTP1 expression slightly increased after 24 h incubation (1.08-fold) while the expressions of isoform GSTT2 and GSTM2 were below the limit of detection. GSH sharply decreased, suggesting the formation of GSH-SR adducts that may have an impact SR availability. Those results suggest that a regular exposure to SR by broccoli consumption or SR supplements may enhance Glo1.

Age-Related Changes of Beta-Endorphin and Cholecystokinin in Peripheral Blood Mononuclear Cells

1991 ◽  
Vol 621 (1 Physiological) ◽  
pp. 174-178 ◽  
Author(s):  
ALBERTO E. PANERAI ◽  
BARBARA MANFREDI ◽  
LUISA LOCATELLI ◽  
FRANCESCA RUBBOLI ◽  
PAOLA SACERDOTE
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Beta Endorphin ◽  
Age Related ◽  
Blood Mononuclear Cells ◽  
Age Related Changes

Age-related impaired proliferation of peripheral blood mononuclear cells is associated with an increase in both IL-10 and IL-12

1999 ◽  
Vol 34 (2) ◽  
pp. 243-252 ◽  
Author(s):  
Steven C Castleab ◽  
Koichi Uyemuraabc ◽  
William Crawford ◽  
Wendy Wongab ◽  
W.B Klaustermeyer ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Age Related ◽  
Blood Mononuclear Cells

Age-related fatigue is associated with reduced mitochondrial function in peripheral blood mononuclear cells

2021 ◽  
Vol 144 ◽  
pp. 111177
Author(s):  
Catrin Herpich ◽  
Kristina Franz ◽  
Susanne Klaus ◽  
Ursula Müller-Werdan ◽  
Mario Ost ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Mitochondrial Function ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Age Related ◽  
Blood Mononuclear Cells

scholarly journals Age-related epigenetic drift deregulates SIRT6 expression and affects its downstream genes in human peripheral blood mononuclear cells

Epigenetics ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. 1336-1347
Author(s):  
Magdalena Owczarz ◽  
Jacek Połosak ◽  
Anna Domaszewska-Szostek ◽  
Paulina Kołodziej ◽  
Alina Kuryłowicz ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Age Related ◽  
Blood Mononuclear Cells
Sign in / Sign up

Export Citation Format

Share Document