scholarly journals Dendrophthoe pentandra Induced Apoptosis and Cell Cycle Arrest at G1/S in Human Breast Adenocarcinoma Cells, MCF-7 via Up- Regulation of p53

2018 ◽  
Vol 8 (9) ◽  
pp. 130-141
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Cycle Arrest ◽  
Adenocarcinoma Cells ◽  
Induced Apoptosis ◽  
Mcf 7

Casuarinin from the Bark ofTerminalia arjunaInduces Apoptosis and Cell Cycle Arrest in Human Breast Adenocarcinoma MCF-7 Cells

Planta Medica ◽  
2005 ◽  
Vol 71 (3) ◽  
pp. 237-243 ◽  
Author(s):  
Po-Lin Kuo ◽  
Ya-Ling Hsu ◽  
Ta-Chen Lin ◽  
Liang-Tzung Lin ◽  
Jiunn-Kae Chang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Cycle Arrest ◽  
Mcf 7

scholarly journals Induction of Apoptosis and Cytotoxicity by Raphasatin in Human Breast Adenocarcinoma MCF-7 Cells

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3092 ◽  
Author(s):  
Muhammad Ibrahim ◽  
Saie Kntayya ◽  
Nooraini Mohd Ain ◽  
Renato Iori ◽  
Costas Ioannides ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Chromatin Condensation ◽  
Annexin V ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Cycle Arrest ◽  
Induction Of Apoptosis ◽  
Mcf 7

Glucoraphasatin (GRH), a glucosinolate present abundantly in the plants of the Brassicaceae family, is hydrolyzed by myrosinase to raphasatin, which is considered responsible for its cancer chemopreventive activity; however, the underlying mechanisms of action have not been investigated, particularly in human cell lines. The aims of this study are to determine the cytotoxicity of raphasatin, and to evaluate its potential to cause apoptosis and modulate cell cycle arrest in human breast adenocarcinoma MCF-7 cells. The cytotoxicity was determined following incubation of the cells with glucoraphasatin or raphasatin (0–100 µM), for 24, 48, and 72 h. GRH displayed no cytotoxicity as exemplified by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. When myrosinase was added to the incubation system to convert GRH to raphasatin, cytotoxicity was evident. Exposure of the cells to raphasatin stimulated apoptosis, as was exemplified by cell shrinkage, membrane blebbing, chromatin condensation, and nuclear fragmentation. Moreover, using Annexin V-FITC assay, raphasatin induced apoptosis, as witnessed by changes in cellular distribution of cells, at different stages of apoptosis; in addition, raphasatin caused the arrest of the MCF-7 cells at the G2 + M phase. In conclusion, raphasatin demonstrated cancer chemopreventive potential against human breast adenocarcinoma (MCF-7) cells, through induction of apoptosis and cell cycle arrest.

scholarly journals Ajwa Date (Phoenix dactylifera L.) Extract Inhibits Human Breast Adenocarcinoma (MCF7) Cells In Vitro by Inducing Apoptosis and Cell Cycle Arrest

PLoS ONE ◽  
2016 ◽  
Vol 11 (7) ◽  
pp. e0158963 ◽  
Author(s):  
Fazal Khan ◽  
Farid Ahmed ◽  
Peter Natesan Pushparaj ◽  
Adel Abuzenadah ◽  
Taha Kumosani ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Phoenix Dactylifera ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Mcf7 Cells ◽  
Human Breast Adenocarcinoma ◽  
Cycle Arrest ◽  
Phoenix Dactylifera L

The mechanism of ellipticine-induced apoptosis and cell cycle arrest in human breast MCF-7 cancer cells

2005 ◽  
Vol 223 (2) ◽  
pp. 293-301 ◽  
Author(s):  
Po-Lin Kuo ◽  
Ya-Ling Hsu ◽  
Cheng-Hsiung Chang ◽  
Chun-Ching Lin
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
Human Breast ◽  
Cycle Arrest ◽  
Induced Apoptosis ◽  
Mcf 7

scholarly journals Change in the Expression of BAK، FAS، BAX، TNF-A، BCL-2 and Survivin Apoptosis Genes of the Human Breast Adenocarcinoma Cells (MCF-7) by Staphylococcal Enterotoxin B

2019 ◽  
Author(s):  
Sara Afzali ◽  
Abbas Doosti ◽  
Mansour Heidari ◽  
Nahid Babaei ◽  
Parvaneh Keshavarz
Keyword(s):  
Staphylococcal Enterotoxin ◽  
Breast Adenocarcinoma ◽  
Control Group ◽  
Staphylococcal Enterotoxin B ◽  
Type B ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Adenocarcinoma Cells ◽  
Enterotoxin B ◽  
Mcf 7

Introduction: Breast cancer is one of the most prevalent malignancies among women. Patients whose suffering from this condition, as a result of the use of conventional therapies often have a poor response to treatment and the relapse among them is frequent. In this study, the effects of staphylococcal enterotoxin type B on BAK، FAS، BAX، TNF-a، BCL-2 و Survivin genes expression in human breast adenocarcinoma cells (MCF-7) was examined. Staphylococcal enterotoxin B is a powerful member of the Staphylococcus aureus toxins family, which is known as an anticancer agent with potential for killing cancer cells. Methods: The experimental study was carried out at the Biotechnology Research Center of Islamic Azad University, Shahrekord Branch. By using Lipofectamine 2000 reagent, MCF-7  cells transfected with the pcDNA3.1(+)-seb (recombinant) and  pcDNA3.1(+) (non-recombinant)  plasmids and were selected by culturing in a selective medium of RPMI- 1640 containing 600 μg / mL antibiotic G418. Then, the expression of BAK, FAS, BAX, TNF-a, BCL-2, and Survivin genes in transfected cells were analyzed by real time PCR. Student's t-test, SPSS Inc., Chicago, IL; Version 16 and also Excel program for statistical analysis were used. Results: The results of this study indicated that staphylococcal enterotoxin type B (SEB) remarkably changes the expression of apoptotic related genes in MCF-7 cell line. It was observed a significant increase in the expression of BAK, FAS, BAX, and TNF-a genes, the expression of BCL-2 and Survivin genes significantly decreased compared to the control group (P=0/032). Conclusion: Staphylococcal enterotoxin type B has an inhibitory effect on the growth, proliferation and invasion of breast adenocarcinoma cells through altering the expression of the genes involved in the apoptosis process. Therefore, it seems that there is a good research field for the use of this toxin in the control and treatment of human breast adenocarcinoma.

Sign in / Sign up

Export Citation Format

Share Document