scholarly journals Mango mistletoe Dendrophthoe pentandra leaf extract acts synergistically with 5-Fluorouracil to induce apoptosis and increase p21 expression in human cervical adenocarcinoma HeLa cells by reducing survivin expression

2018 ◽  
Vol 8 (7) ◽  
pp. 10-15 ◽  
Keyword(s):  
Hela Cells ◽  
Leaf Extract ◽  
Survivin Expression ◽  
Cervical Adenocarcinoma

Effects of Papaya Leaf Extract (Carica papaya L.) on Cellular Proliferation and Apoptosis in Cervical Cancer Mice Model

2019 ◽  
Vol 17 (5) ◽  
pp. 265-275
Author(s):  
Y. Peristiowati ◽  
Y. Puspitasari ◽  
Indasah
Keyword(s):  
Cervical Cancer ◽  
Cell Proliferation ◽  
Hela Cells ◽  
Leaf Extract ◽  
Caspase 3 ◽  
Methanol Extract ◽  
Negative Control ◽  
Proliferation Index ◽  
Control Group ◽  
P53 Expression

This study is aimed at analyzing the anticancer properties of papaya leaf extract, specifically the inhibition of cell proliferation and apoptotic induction through nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and p53 pathways. Twenty-five mice (Mus musculus), aged 2 months and weighing 20–30 g, was injected with 0.5 mg dexamethasone for 7 days. The mice were then injected intracutaneously with 1 ml of HeLa cells (8 × 106 HeLa cells/microliter). The mice were divided into five groups (5 each): negative control (P1) (5% CMC-Na, sodium carboxymethyl cellulose), treatment II (225 mg/kg BW (body weight) papaya leaves methanol extract), treatment III (450 mg/kg BW), treatment IV (750 mg/kg BW), and treatment PV (2 mg alcohol anticancer drug). Papaya leaf extract treatments were applied for 2 weeks. Then, the tumor tissue was isolated for hematoxylin and eosin staining. Immunohistochemical imaging was used to detect Ki-67, caspase-3, NF-κB, and p53 expression. Further analysis was undertaken using the ImmunoRatio software program. The results indicated that administration of papaya leaf methanol extract significantly increased the expression of NF-κB and p53 at a dose of 450 mg/kg BW. Our results also showed that the mice treated with 450 mg of papaya leaf extract per kg of BW (P3) had the largest increase of caspase-3 expression compared to the negative control group. Papaya leaf ethanol extract decreased the cancer cell proliferation index and increased apoptosis of cancer cells in animal models of cervical cancer; it may also work to increase NF-kB expression and expression of the p53 gene.

New Hybrid Scaffolds Based on Carbazole-Chalcones as Potent Anticancer Agents

2020 ◽  
Vol 20 ◽  
Author(s):  
Faisal Rashid ◽  
Sumera Zaib ◽  
Aliya Ibrar ◽  
Syeda Abida Ejaz ◽  
Aamer Saeed ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Hela Cells ◽  
Cervical Adenocarcinoma ◽  
G1 Arrest ◽  
Human Breast ◽  
Caspase 9 ◽  
Flow Cytometric ◽  
Pharmacodynamic Profile ◽  
Microscopic Studies ◽  
Mcf 7

Background and Objectives: Despite various technological advances for the treatment of cancer, the identification of new chemical entities with potent anticancer effects remain an indispensable requirement of the time due to multi-drug resistance exhibited by previously developed anticancer drugs. Particularly, the hybrid drugs incorporating two individual bioactive pharmacophores present medicinally important structural leads, thus improving the pharmacodynamic profile of the drug molecules. The antiproliferative and pro-apoptotic activity of the carbazole-chalcone hybrids on human breast and cervical cancer cells will be examined. Materials and Methods: To overcome such complications, in the current study, we evaluated the cytotoxic effects of carbazole-chalcone hybrids on human breast adenocarcinoma (MCF-7), cervical adenocarcinoma (HeLa) cells and normal cells i.e., baby hamster kidney cells (BHK-21) using MTT (dimethyl-2-thiazolyl-2,5-diphenyl-2H-tetrazolium bromide) assay. The mechanistic studies were performed on potent compound 4g by fluorescent microscopic studies, release of lactate dehydrogenase (LDH) and mitochondrial membrane potential, activation of caspase-9 and -3 and flow cytometric analysis. Results: As revealed by MTT assay, compound 4g was identified as the most potent derivative among the tested series with IC50 values of 5.64 and 29.15 μM against HeLa and MCF-7 cells, respectively. The results were compared with cisplatin. Fluorescent microscopic studies using 4′,6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI) staining confirmed the occurrence of apoptosis in HeLa cells treated with the most active compound 4g. Moreover, compound 4g also triggered the release of lactate dehydrogenase (LDH) in treated HeLa and MCF-7 cells while a luminescence assay displayed a remarkable increase in the activity of caspase-9 and -3. Moreover, flow cytometric results revealed that compound 4g caused G0/G1 arrest in the treated HeLa cells. Conclusion: Our results demonstrated that the compound 4g possesses chemotherapeutic properties against breast cancer and cervical adenocarcinoma cells, thus warranting further research to test the anticancer efficacy of this compound at clinical level.

scholarly journals The in vitro effects of compound-X on growth, morphology, the induction of autophagy and apoptosis, as well as cell cycle progression in a cervical adenocarcinoma cell line

2012 ◽  
Vol 31 (1) ◽  
Author(s):  
S. Marais ◽  
T.V. Mqoco ◽  
B.A. Stander ◽  
R. Prudent ◽  
L. Lafanechère ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Line ◽  
Hela Cells ◽  
Cell Cycle Progression ◽  
Cervical Adenocarcinoma ◽  
Growth Morphology ◽  
Adenocarcinoma Cell Line ◽  
Cycle Progression ◽  
Adenocarcinoma Cell

It can be concluded that compound-X induced both autophagy and apoptosis as a means of celldeath in HeLa cells.

scholarly journals Crinane Alkaloids of the Amaryllidaceae with Cytotoxic Effects in Human Cervical Adenocarcinoma (HeLa) Cells

2014 ◽  
Vol 9 (4) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Jerald J. Nair ◽  
Lucie Rárová ◽  
Miroslav Strnad ◽  
Jaume Bastida ◽  
Lee Cheesman ◽  
...  
Keyword(s):  
South Africa ◽  
Indigenous Peoples ◽  
Hela Cells ◽  
Cervical Adenocarcinoma ◽  
Cytotoxic Activities ◽  
Adenocarcinoma Cell Line ◽  
Cytotoxic Effects ◽  
Adenocarcinoma Cell ◽  
The Family ◽  
History Of

The family Amaryllidaceae has a long history of usage in the traditional medicinal practices of the indigenous peoples of South Africa, with three of its species known to be used for cancer treatment. Furthermore, the Amaryllidaceae is widely recognized for its unique alkaloid constituents, several of which exhibit potent and selective cytotoxic activities. In this study, several crinane alkaloids derived from local Amaryllidaceae species were examined for cytotoxic effects against the human cervical adenocarcinoma cell line, of which distichamine was the most potent (IC50 2.2 μM).

Antiproliferative and apoptosis inducing activity of Markhamia tomentosa leaf extract on HeLa cells

2013 ◽  
Vol 149 (3) ◽  
pp. 745-749 ◽  
Author(s):  
Bolanle Ibrahim ◽  
Abimbola Sowemimo ◽  
Luanne Spies ◽  
Trevor Koekomoer ◽  
Maryna van de Venter ◽  
...  
Keyword(s):  
Hela Cells ◽  
Leaf Extract

scholarly journals Rapid Biosynthesis of Silver Nanoparticles Using Pepino (Solanum muricatum) Leaf Extract and Their Cytotoxicity on HeLa Cells

Materials ◽  
2016 ◽  
Vol 9 (5) ◽  
pp. 325 ◽  
Author(s):  
Mónica Gorbe ◽  
Ravishankar Bhat ◽  
Elena Aznar ◽  
Félix Sancenón ◽  
M. Marcos ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Hela Cells ◽  
Leaf Extract ◽  
Solanum Muricatum

On the mechanism of phenotypic conversion of human cervical adenocarcinoma HeLa cells surviving infection by influenza B virus: Potential implications for biological management of adenocarcinomas

2008 ◽  
Vol 204 (5) ◽  
pp. 329-334 ◽  
Author(s):  
Despina O. Sioutopoulou ◽  
Elias Plakokefalos ◽  
Leonidas Arvanitis ◽  
Evangelos Athanassiou ◽  
John Venizelos ◽  
...  
Keyword(s):  
Hela Cells ◽  
Cervical Adenocarcinoma ◽  
Influenza B Virus ◽  
Influenza B ◽  
B Virus ◽  
Biological Management

scholarly journals EVALUATION OF THE ANTICANCER PROPERTIES OF TURMESAC® IN HUMAN CERVICAL ADENOCARCINOMA

2020 ◽  
pp. 109-113
Author(s):  
FIROZ HM ◽  
NANJUNDAIAH S ◽  
SADASHIVA CT
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Hela Cells ◽  
Anticancer Agent ◽  
Cervical Adenocarcinoma ◽  
Apoptosis Induction ◽  
Anticancer Properties ◽  
Cycle Arrest ◽  
Diphenyltetrazolium Bromide ◽  
Ic50 Value

Objective: In the current study, an extract of turmeric rhizome (Turmesac®) was evaluated for possible anticancer activity in human cervical adenocarcinoma (HeLa) cells. Methods: Turmesac®’s ability to elicit cytotoxicity in cancer cells was evaluated by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay (MTT) assay, where the IC50 value was determined. Apoptosis induction and cell cycle arrest were analyzed by flow cytometry post-Turmesac® IC50 value treatment for 24 h. Results: The determined IC50 value of Turmesac® in HeLa cells was 115.12 μg/ml. This concentration was able to induce apoptosis 2 times greater than the apoptotic standard, camptothecin, treated cells. Cell cycle arrest was observed at the G0/G1 and S phases in Turmesac® treated HeLa cells. Conclusion: Turmesac® shows the potential of being a promising anticancer agent that may be incorporated into chemotherapies, but further study is required to elucidate the exact mechanisms involved with longer treatment duration, as would be the case in clinical trial phases.

scholarly journals Corrigendum to: Screening of Ipomoea tuba Leaf Extract for Identification of Bioactive Compounds and Evaluation of Its in vitro Antiproliferative Activity Against MCF-7 and HeLa Cells (Published: Food Technol. Biotechnol. 58 (1) 71-75 (2020) https://doi.org/10.17113/ftb.58.01.20.6351)

2020 ◽  
Vol 58 (3) ◽  
pp. 356-356
Author(s):  
Thirupati Chinna Venkateswarulu ◽  
Gaddam Eswaraiah ◽  
Srirrama Krupanidhi ◽  
Karlapudi Abraham Peele ◽  
Indira Mikkili ◽  
...  
Keyword(s):  
Cell Lines ◽  
Bioactive Compounds ◽  
Hela Cells ◽  
Antiproliferative Activity ◽  
Leaf Extract ◽  
Mcf 7

The authors requested the replacement of Fig. 1. Morphology of MCF-7 cells after the treatment with the extract of Ipomoea tuba leaf: a) untreated MCF-7 cell lines, and b-g) treated with different concentrations (5, 10, 25, 50, 75 and 100 μg/mL respectively) of the leaf extract The change includes the replacemend of images 1c-1g.

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