scholarly journals Adverse influence of ß-methylcholanthrene on detoxification function of chick embryo brain glutathione S-transferases and degenerative changes of brain

2018 ◽  
Keyword(s):  
Chick Embryo ◽  
Degenerative Changes ◽  
Glutathione S Transferases ◽  
Adverse Influence ◽  
Embryo Brain

scholarly journals SDS/PAGE characteristics of protein kinases tightly associated with chick embryo brain ribosomes.

1999 ◽  
Vol 46 (4) ◽  
pp. 911-917
Author(s):  
M Sanecka-Obacz
Keyword(s):  
Chick Embryo ◽  
Protein Kinases ◽  
Embryo Development ◽  
Sds Page ◽  
Casein Kinases ◽  
Triton X ◽  
Brain Ribosomes ◽  
Embryo Brain ◽  
Exogenous Substrates

Protein kinases tightly associated with chick embryo brain ribosomes washed with Triton X-100 and KCl were characterized by their ability to phosphorylate ribosomes and two exogenous substrates, histone IIA and casein. c-AMP-dependent kinase (PKA) and casein kinases (CK1, CK2) were examined in the presence of specific modulators by SDS/PAGE followed by renaturation in gel assay according to Kameshita & Fujisawa (Anal. Biochem. 1989, 183, 139-143). Basing on these data it can be presumed that PKA activity increases, but the levels of CK2 and CK1 decrease during chick embryo development.

The cellular environment controls the expression of engrailed-like protein in the cranial neuroepithelium of quail-chick chimeric embryos

Development ◽  
1991 ◽  
Vol 113 (3) ◽  
pp. 1037-1048 ◽  
Author(s):  
C.A. Gardner ◽  
K.F. Barald
Keyword(s):  
Chick Embryo ◽  
Protein Expression ◽  
Morphological Changes ◽  
Polyclonal Antiserum ◽  
Negative Region ◽  
Cellular Environment ◽  
Normal Environment ◽  
Restricted Region ◽  
Embryo Brain

We have previously shown that one of two chicken engrailed-like genes, chick En-2, is expressed in a restricted region of the early chick embryo brain: the mes/metencephalon (Gardner et al. 1988). In this study, we examine the role of the cellular environment in regulation of engrailed-like (En) protein expression in quail-chick chimeric embryos. Two types of transplant surgery were performed at the 9–15 somite stage to produce chimeric embryos. In the first, the mid-mesencephalic vesicle or caudal mesencephalic vesicle alar plate (which is En protein-positive) was transplanted from a quail embryo into an En protein-negative region of chick neuroepithelium, the prosencephalon (mMP and cMP grafts, respectively). In the second reciprocal surgery, prosencephalic alar plate which is En protein-negative, was transplanted into the En protein-positive mesencephalic vesicle (PM grafts). A polyclonal antiserum, alpha Enhb-1, which recognizes chick En proteins (Davis et al. 1991) was used to identify En-positive cells 48 h after surgery. In mMP embryos, 71% of integrated grafts had lost En expression (n = 17). In contrast, in cMP grafts, 93% of integrated grafts continued to stain with the antiserum (n = 14). In addition, in 86% of these embryos, the graft induced adjacent chick host diencephalic cells to become En protein-positive as well. All PM grafts contained aEnhb-1-positive cells; such cells never expressed this protein in their normal environment. These early changes in En protein expression correlate well with the morphological changes observed in similar graft surgeries assayed later in development. Thus, our results are consistent with the hypothesis that En genes play a role in the regionalization of the early cranial neuroepithelium.

L-type calcium channels may regulate neurite initiation in cultured chick embryo brain neurons and N1E-115 neuroblastoma cells

1990 ◽  
Vol 55 (1) ◽  
pp. 109-120 ◽  
Author(s):  
G. Audesirk ◽  
T. Audesirk ◽  
C. Ferguson ◽  
M. Lomme ◽  
D. Shugarts ◽  
...  
Keyword(s):  
Chick Embryo ◽  
Calcium Channels ◽  
Neuroblastoma Cells ◽  
Brain Neurons ◽  
Neurite Initiation ◽  
Embryo Brain

The translation of rabbit hemoglobin messenger RNA in a cell-free extract from chick embryo brain

1974 ◽  
Vol 36 (2) ◽  
pp. 299-310 ◽  
Author(s):  
Don Hendrick ◽  
Walter Knöchel ◽  
Walter Schwarz ◽  
Sabine Pitzel ◽  
Heinz Tiedemann
Keyword(s):  
Chick Embryo ◽  
Messenger Rna ◽  
Cell Free Extract ◽  
A Cell ◽  
Embryo Brain

scholarly journals Isolation and separation of α and β-tubulin from chick-embryo brain, muscle and skin

1978 ◽  
Vol 169 (3) ◽  
pp. 717-719
Author(s):  
A R A el-Hamalawi
Keyword(s):  
Chick Embryo ◽  
Sodium Dodecyl Sulphate ◽  
Sodium Dodecyl ◽  
Disc Electrophoresis ◽  
Polyacrylamide Gel ◽  
New Method ◽  
Embryo Brain ◽  
Β Tubulin

Two kinds of tubulin, alpha and beta, have been described in microtubules from many different systems. In the present study a new method is described for isolating and separating these two kinds of tubulin from chick-embryo brain, muscle and skin. The isolated tubulins were characterized by polyacrylamide-gel disc electrophoresis in the presence of urea and sodium dodecyl sulphate.

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