scholarly journals Validation of analytical method by HPLC for determination of dapsone in polymeric nanocapsules based on crude rice brain oil

2017 ◽  
Keyword(s):  
Analytical Method ◽  
Polymeric Nanocapsules ◽  
Validation Of Analytical Method

scholarly journals Development and validation of analytical method by HPLC-DAD for determination of vasodilator active in pharmaceutical ophthalmic forms

2020 ◽  
Vol 41 ◽  
Author(s):  
Aina Liz Alves Cesar ◽  
Larissa Caldeira Navarro ◽  
Alaíde de Matos Silva ◽  
Rachel Oliveira Castilho ◽  
Gisele Assis Castro Goulart ◽  
...  
Keyword(s):  
Analytical Method ◽  
Development And Validation ◽  
Validation Of Analytical Method

scholarly journals Development and Validation of Analytical Method for Determination of an Important Flavonoid in Malva neglecta

2019 ◽  
pp. 1-8
Author(s):  
Naira Nayeem Akmal ◽  
Mohd. Imran ◽  
Mohammad Ali Mujtaba
Keyword(s):  
Quality Control ◽  
Calibration Curve ◽  
Analytical Method ◽  
Methanolic Extract ◽  
Hplc Method ◽  
Average Recovery ◽  
Acceptance Criterion ◽  
Development And Validation ◽  
Validation Of Analytical Method

Development of genuine and dependable analytical methods which profile marker phytoconstituents in   an extract containing a mixture of several components is a challenging task. A simple, rapid, precise, and reliable HPLC method was developed for quantification of quercetin from the methanolic extract of Malva neglecta. The estimation was carried out using Phenomenex Gemini-NX-5 µm C18 .The parameters  considered for validation were accuracy, precision, linearity and robustness. The calibration curve was found to be linear in a concentration range of 20–100 μg/mL.The correlation coefficient was r2=0.9996. The % average recovery of quercetin was found to be in the range of 99.82 to 100.52% which was within the acceptance criterion indicating the accuracy of the method. The results of the robustness study indicated that there is no influence of minor changes. The developed and validated method can be successfully used for the determination of quercetin in Malva neglecta; thereby helping in authentication and quality control of this plant.

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