scholarly journals Modulatory effect of berberine on adipose tissue PPARγ, adipocytokines and oxidative stress in high fat diet/ streptozotocin-induced diabetic rats

2017 ◽  
Keyword(s):  
Oxidative Stress ◽  
Adipose Tissue ◽  
High Fat Diet ◽  
Diabetic Rats ◽  
High Fat ◽  
And Oxidative Stress

scholarly journals Countering adipose tissue dysfunction could underlie the superiority of telmisartan in the treatment of obesity-related hypertension

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Yahya M. Naguib ◽  
Rehab M. Samaka ◽  
Mohamed S. Rizk ◽  
Omnia Ameen ◽  
Shaimaa M. Motawea
Keyword(s):  
Oxidative Stress ◽  
Adipose Tissue ◽  
Visceral Adipose Tissue ◽  
High Fat Diet ◽  
Serum Lipids ◽  
High Fat ◽  
Diet Induced Obesity ◽  
Adipose Tissue Dysfunction ◽  
Visceral Adipose ◽  
And Oxidative Stress

Abstract Background The prevalence of hypertension and obesity has increased significantly in recent decades. Hypertension and obesity often coexist, and both are associated with increased cardiovascular mortality. Obese hypertensive patients usually require special anti-hypertensive treatment strategy due to the increased risk of treatment resistance. Molecules that can target both obesity and hypertension underlying pathologies should get more attention. Herein, we evaluated the therapeutic effects of telmisartan, with special interest in visceral adipose tissue dysfunction, in obesity-related hypertension rat model. Methods Thirty male Wistar rats weighing 150–200 g were equally divided into: 1—Control group (fed normal laboratory diet for 24 weeks), 2—Diet-induced obesity group (DIO, fed high fat diet for 24 weeks), and 3—Diet-induced obesity treated with telmisartan group (DIO + Tel, fed high fat diet and received telmisartan for 24 weeks). At the end of the study, anthropometrical parameters were evaluated. Systolic blood pressure and heart rate were measured. Blood samples were collected for the measurement of serum lipids, adipokines, cardiac, renal, inflammatory, and oxidative stress biomarkers. Kidneys were removed and used for histopathological studies, and visceral adipose tissue was utilized for histopathological, immunohistochemical and RT-PCR studies. Results High fat diet resulted in obesity-related changes in anthropometrical parameters, elevation of blood pressure, increase in heart rate, higher serum levels of cardiac, inflammatory and kidney function biomarkers, with altered serum lipids, adipokines and oxidative stress markers. Morphological changes (H&E and PAS-stained sections) were noticed in kidneys and visceral adipose tissue. Immunohistochemistry and RT-PCR studies confirmed adipose tissue dysfunction and over-expression of inflammatory and oxidative stress proteins. Telmisartan countered obesity-induced alterations in cardiovascular, renal, and adipose tissue functions. Conclusion Adipose tissue dysfunction could be the core pathophysiology of obesity-related hypertension. Besides its anti-hypertensive effect, telmisartan had profound actions on visceral adipose tissue structure and function. Attention should be given to polymodal molecules targeting adipose tissue-related disorders.

scholarly journals The Effects of Quercetin on High Fat Diet-Induced Inflammation and Oxidative Stress in Brown Adipose Tissue

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1669-1669
Author(s):  
Ya Pei ◽  
Hye Won Kang
Keyword(s):  
Oxidative Stress ◽  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
High Fat Diet ◽  
The Body ◽  
Low Grade ◽  
Related Factor ◽  
High Fat ◽  
Brown Adipose ◽  
And Oxidative Stress

Abstract Objectives Under obesity state, adipose tissue derived inflammatory mediators circulate all over the body and induce low-grade chronic inflammation, which is the main cause for the development of metabolic diseases. Moreover, inflammation-induced reactive oxygen species (ROS) cause oxidative stress, a process in damaging cellular structure and functions. Recently, microRNAs (miRNAs) were found to potentially regulate inflammation and its associated diseases. Brown adipose tissue (BAT) protects against obesity through thermogenic activity to increase energy expenditure. However, high levels of inflammation, ROS generation and aberrant level of miRNAs result in the dysfunction of BAT. Previously, quercetin showed anti-obesity effect through BAT activation. Thus, the purpose of this study was sought to investigate the effect of quercetin on high fat diet (HFD)-induced inflammation and oxidative stress in BAT. Methods C57BL/6 male mice were fed with a HFD or HFD supplemented with 1% quercetin (HFDQ) for 16 weeks. Total RNA was isolated from BAT to measure the expression of target mRNAs such as tumor necrosis factor alpha (TNFa), interleukin (IL) 1b, IL6, inducible nitric oxide synthase (iNOS), cyclooxygenase (COX) 2, NADPH oxidase (NOX) 2, nuclear factor E2-related factor (NRF) 2, superoxide dismutase (SOD) 2, SOD3, and catalase that are involved in regulation of inflammation and oxidative stress, and microRNA (miRNA)-155, a master regulator of inflammation, using a quantitative PCR. Results BAT of HFDQ-fed mice exhibited decreased expression of COX2, TNFa, IL1b, IL6, and iNOS compared to that of HFD-induced obese mice. NOX2 gene encoding an enzyme that generates ROS was also decreased in BAT of HFDQ-fed mice. The genes such as SOD2, SOD3, NRF2, and catalase that are involved in regulation of antioxidant enzymes were significantly increased. As the cognate gene of TNFa, miRNA-155 levels were downregulated. Conclusions Quercetin ameliorates HFD-induced inflammation and oxidative stress in BAT by regulating miRNA-155. Intake of quercetin may improve obese conditions by regulating BAT function through anti-inflammatory and antioxidant effects. Funding Sources This work was supported by USDA.

scholarly journals Histidine supplementation alleviates inflammation in the adipose tissue of high-fat diet-induced obese rats via the NF-κB- and PPARγ-involved pathways

2014 ◽  
Vol 112 (4) ◽  
pp. 477-485 ◽  
Author(s):  
Xiaowei Sun ◽  
Rennan Feng ◽  
Yanchuan Li ◽  
Song Lin ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adipose Tissue ◽  
High Fat Diet ◽  
Low Grade ◽  
Obese Women ◽  
Sprague Dawley ◽  
High Fat ◽  
Tnf Α ◽  
Obese Rats ◽  
And Oxidative Stress

Obesity is considered to be accompanied by a chronic low-grade inflammatory state that contributes to the occurrence of many chronic diseases. Our previous study has demonstrated that histidine supplementation significantly ameliorates inflammation and oxidative stress in obese women. However, the in vivo potential mechanisms are not known. The present study was conducted to investigate the mechanisms underlying the effects of histidine on inflammation in a high-fat diet (HFD)-induced female obese rat model. An obese model was established in female Sprague–Dawley rats by HFD feeding for 8 weeks and followed by histidine supplementation for another 4 weeks. The results revealed that HFD-increased body weight and HFD-lowered serum histidine concentrations were significantly reversed by histidine supplementation (P< 0·05). In addition, the serum concentrations of TNF-α, IL-6, C-reactive protein (CRP) and malondialdehyde were significantly reduced and those of superoxide dismutase (SOD) were significantly increased by histidine supplementation when compared with those in obese rats (P< 0·05). Correspondingly, the mRNA expressions of TNF-α, IL-6 and CRP in the adipose tissue were significantly down-regulated and that of CuZnSOD was significantly up-regulated by histidine supplementation (P< 0·05). Histidine supplementation significantly reduced the HFD-induced translocation of NF-κB p65 into the nucleus (P= 0·032) by reducing the phosphorylation of the inhibitor of κBα in the adipose tissue. The results also revealed that the expression of adiponectin was markedly increased both in the serum and in the adipose tissue after histidine supplementation, accompanied by the activation of PPARγ (P= 0·021). These findings indicate that histidine is an effective candidate for ameliorating inflammation and oxidative stress in obese individuals via the NF-κB- and PPARγ-involved pathways.

scholarly journals Calorie Restriction with a High-Fat Diet Effectively Attenuated Inflammatory Response and Oxidative Stress-Related Markers in Obese Tissues of the High Diet Fed Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Seungae Park ◽  
Na-Young Park ◽  
Giuseppe Valacchi ◽  
Yunsook Lim
Keyword(s):  
Oxidative Stress ◽  
Adipose Tissue ◽  
Systemic Inflammation ◽  
Calorie Restriction ◽  
High Fat Diet ◽  
Heme Oxygenase 1 ◽  
High Fat ◽  
Expression Levels ◽  
Diet Induced Obesity ◽  
And Oxidative Stress

Obesity characterized by increased mass of adipose tissue leads to systemic inflammation. Calorie restriction (CR) improves parameters associated with immune response and antioxidant defense. We hypothesized that CR with a high fat diet (HFCR) regulates local and systemic inflammation and oxidative stress damage in a high fat diet induced obesity (HF group). We investigated effect of HFCR on inflammation and oxidative stress-related markers in liver and adipose tissues as well as adipokines in plasma. HFCR lowered liver triglyceride levels, total cholesterol levels, and the plasma leptin/adiponectin ratio to normal levels and improved glucose tolerance. HFCR also improved fatty liver and normalized adipocyte size and morphology. HFCR reduced lipid peroxidation and decreased the expression levels of inducible nitric oxide synthetase, cyclooxygenase-2, NF-E2-related factor, and heme oxygenase-1 in the liver. Moreover, HFCR suppressed the expression levels of C- reactive protein and manganese superoxide dismutase in the adipose tissue in the HF group. These results suggest that HFCR may have beneficial effects on inflammation and oxidative stress as well as lipid profiles in the HF diet induced obesity. Moreover, HFCR may be a good way to increase compliance in obese patients and to prevent obesity induced complications without changes in dietary pattern.

scholarly journals Amelioration of Obesity, Glucose Intolerance, and Oxidative Stress in High-Fat Diet and Low-Dose Streptozotocin-Induced Diabetic Rats by Combination Consisting of “Curcumin with Piperine and Quercetin”

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Ginpreet Kaur ◽  
Meena C
Keyword(s):  
Oxidative Stress ◽  
Glucose Intolerance ◽  
High Fat Diet ◽  
Low Dose ◽  
Diabetic Rats ◽  
High Fat ◽  
Medicinal Uses ◽  
Induced Changes ◽  
And Oxidative Stress

Curcumin is an important nutraceutical that has enormous potential for a variety of diseases, but the medicinal properties of curcumin cannot be utilized due to its low in vivo bioavailability. Therefore, in view of the foregoing, there is an extensive need for combinatorial extract “curcumin with piperine and quercetin” which may enhance bioavailability of oral curcumin by inhibiting the enzymes responsible for the metabolism of curcumin. Thus, the present study investigated the effect of combinatorial extract of curcumin on obesity, glucose intolerance, and oxidative stress in high fat diet and low-dose streptozotocin-induced rats. Oral administration of combinatorial extract for 28 days significantly () reduced PGL (64.84%), PTG (88.94%), LDL (26.38%) and PTC (50.23%) levels, respectively and improved glucose tolerance () significantly to exogenously administered glucose (2 g/kg) at 60, 90, and 120 min interval on OGTT. The results for antioxidant potential indicate that at 100 mg/kg dose of combinatorial extract of curcumin significantly prevented the high-fat diet and low-dose streptozotocin-induced changes in the oxidative stress parameters () which supports popular medicinal uses of this combinatorial extract as antihyperglycemic and hypolipidemic and is likely to bring this promising natural product to the forefront of therapeutic agents in the in the treatment of “metabolic syndrome”.

Improvement for High Fat Diet-Induced Hepatic Injuries and Oxidative Stress by Flavonoid-Enriched Extract from Nelumbo nucifera Leaf

2009 ◽  
Vol 57 (13) ◽  
pp. 5925-5932 ◽  
Author(s):  
Ming-Cheng Lin ◽  
Shao-Hsuan Kao ◽  
Pei-Jun Chung ◽  
Kuei-Chuan Chan ◽  
Mon-Yuan Yang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Nelumbo Nucifera ◽  
High Fat ◽  
Hepatic Injuries ◽  
And Oxidative Stress

scholarly journals Effects of a Mixed Limosilactobacillus fermentum Formulation with Claimed Probiotic Properties on Cardiometabolic Variables, Biomarkers of Inflammation and Oxidative Stress in Male Rats Fed a High-Fat Diet

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2202
Author(s):  
Micaelle Oliveira de Luna Freire ◽  
Luciana Caroline Paulino do Nascimento ◽  
Kataryne Árabe Rimá de Oliveira ◽  
Alisson Macário de Oliveira ◽  
Thiago Henrique Napoleão ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Control Diet ◽  
Male Rats ◽  
Control Group ◽  
Low Grade ◽  
Probiotic Properties ◽  
High Fat ◽  
Low Grade Inflammation ◽  
And Oxidative Stress

High-fat diet (HFD) consumption has been linked to dyslipidemia, low-grade inflammation and oxidative stress. This study investigated the effects of a mixed formulation with Limosilactobacillusfermentum 139, L. fermentum 263 and L. fermentum 296 on cardiometabolic parameters, fecal short-chain fatty acid (SCFA) contents and biomarkers of inflammation and oxidative stress in colon and heart tissues of male rats fed an HFD. Male Wistar rats were grouped into control diet (CTL, n = 6), HFD (n = 6) and HFD with L. fermentum formulation (HFD-Lf, n = 6) groups. The L.fermentum formulation (1 × 109 CFU/mL of each strain) was administered twice a day for 4 weeks. After a 4-week follow-up, biochemical parameters, fecal SCFA, cytokines and oxidative stress variables were evaluated. HFD consumption caused hyperlipidemia, hyperglycemia, low-grade inflammation, reduced fecal acetate and propionate contents and increased biomarkers of oxidative stress in colon and heart tissues when compared to the CTL group. Rats receiving the L. fermentum formulation had reduced hyperlipidemia and hyperglycemia, but similar SCFA contents in comparison with the HFD group (p < 0.05). Rats receiving the L. fermentum formulation had increased antioxidant capacity throughout the colon and heart tissues when compared with the control group. Administration of a mixed L. fermentum formulation prevented hyperlipidemia, inflammation and oxidative stress in colon and heart tissues induced by HFD consumption.

Sign in / Sign up

Export Citation Format

Share Document