scholarly journals Phytochemical analysis, Anti inflammatory activity, In vitro antidiabetic activity and GC-MS profile of Erythrina variegata L. bark

2016 ◽  
pp. 147-155 ◽  
Author(s):  
Nagaraj Santhiya ◽  
Suriyamoorthy Priyanga ◽  
Subrhamanian Hemmalakshmi ◽  
Kanakasabapathi Devaki
Keyword(s):  
Inflammatory Activity ◽  
Antidiabetic Activity ◽  
Phytochemical Analysis ◽  
Erythrina Variegata ◽  
Anti Inflammatory

scholarly journals Phytochemical Analysis, Antioxidant and Anti-Inflammatory Activity of Calyces from Physalis peruviana

2014 ◽  
Vol 9 (11) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Reina M. Toro ◽  
Diana M. Aragón ◽  
Luis F. Ospina ◽  
Freddy A. Ramos ◽  
Leonardo Castellanos
Keyword(s):  
Antioxidant Activities ◽  
Folk Medicine ◽  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Physalis Peruviana ◽  
Edema Model ◽  
Anti Inflammatory ◽  
Bio Assay

Physalis peruviana calyces are used extensively in folk medicine. The crude ethanolic extract and some fractions of calyces were evaluated in order to explore antioxidant and anti-inflammatory activities. The anti-inflammatory activity was evaluated by the TPA-induced ear edema model. The antioxidant in vitro activity was measured by means of the superoxide and nitric oxide scavenging activity of the extracts and fractions. The butanolic fraction was found to be promising due to its anti-inflammatory and antioxidant activities. Therefore, a bio-assay guided approach was employed to isolate and identify rutin (1) and nicotoflorin (2) from their NMR spectroscopic and MS data. The identification of rutin in calyces of P. peruviana supports the possible use of this waste material for phytotherapeutic, nutraceutical and cosmetic preparations.

Phytochemical, In vitro Anti-inflammatory and Antimicrobial Potential of Hugonia mystax L.

2021 ◽  
pp. 169-173
Author(s):  
K.P. Jaiganesh ◽  
T.J. Jasna ◽  
A.C. Tangavelou
Keyword(s):  
Tamil Nadu ◽  
Diclofenac Sodium ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Membrane Stabilization ◽  
Traditional System ◽  
Antimicrobial Potential ◽  
Biochemical Compounds ◽  
Anti Inflammatory

Hugonia mystax L., (Linaceae), is commonly distributed in the thorny scrubs and tropical dry evergreen forests of Tamil Nadu, which has been valued for centuries in traditional system of medicine for the treatment of various ailments. In the present study was an attempt to investigate the phytochemical nature and anti-inflammatory, antimicrobial potential by adopting suitable methods. Phytochemical analysis of Hugonia mystax L., plant extracts revealed the presence of various biochemical compounds such as alkaloids, flavonoids, glycosides, triterpenoids and saponins etc. Since triterpenoids and flavonoids have remarkable anti-inflammatory activity, so our present work aims at evaluating in vitro anti inflammatory activity of Hugonia mystax L., by HRBC membrane stabilization method. The inhibition of hypotonicity induced HRBC membrane lysis was taken as a measure of the anti-inflammatory activity. The percentage of membrane stabilization for ethanolic extracts and Diclofenac sodium were done at different concentrations. The maximum membrane stabilization of Hugonia mystax L., extracts was found to be 94.97 % at a dose of 2000 μg/ml. Therefore, our studies support the isolation and the use of active constituents from Hugonia mystax L., in treating inflammations.

scholarly journals IN VITRO ANTI-INFLAMMATORY AND CYTOTOXICITY OF CRINUM X AMABILE GROWN IN ECUADOR

2018 ◽  
Vol 11 (10) ◽  
pp. 99
Author(s):  
Vinueza Diego ◽  
Portero Santiago ◽  
Pilco Gisela ◽  
GarcÍa Marlene ◽  
Acosta Karen ◽  
...  
Keyword(s):  
Total Alkaloid ◽  
Inflammatory Activity ◽  
Tetrazolium Salt ◽  
Phytochemical Analysis ◽  
Total Alkaloids ◽  
Methanolic Extracts ◽  
Anti Inflammatory ◽  
Alkaloid Extracts ◽  
Phytochemical Studies

Objective: The aim of this research was to assess the in vitro anti-inflammatory activity and cytotoxicity of the methanolic extract and total alkaloid extract obtained from leaves and bulbs of Crinum x amabile (CA) growing in Ecuador.Methods: Methanolic extracts of dry powered leaves and bulbs of CA obtained by cold maceration method were subjected to preliminary phytochemical screening. Total alkaloid extracts of leaves and bulbs of CA were obtained by conventional extraction of alkaloids base with an organic solvent. Furthermore, the anti-inflammatory activity and cytotoxicity of the four extracts were investigated by in vitro isolated neutrophils model using stable tetrazolium salt (WST-1).Results: Phytochemical analysis of methanolic extracts revealed the major classes of phytochemicals such as alkaloids, flavonoids, tannins, triterpenes, and steroids, but no proteins or saponins could be detected on leaves extract. Extracts obtained from bulbs both methanolic and total alkaloids of CA show an interesting anti-inflammatory activity, although it was not significant compared to the standard anti-inflammatory drug, aspirin. Cytotoxicity of bulb alkaloid extract was lower compared with all of the other extracts.Conclusion: Based on the results of this research, it could be concluded that CA is a very interesting source of natural anti-inflammatory compounds (especially alkaloids) which could be used to prevent many chronic disorders. Further, phytochemical studies are necessary to identify the chemical compounds responsible for the significant anti-inflammatory activity showed. 

scholarly journals Phytochemical analysis, antioxidant and anti-inflammatory activities of Chassalia kolly leaves extract, a plant used in Benin to treat skin illness

2021 ◽  
Vol 15 (3) ◽  
pp. 063-072
Author(s):  
Koudoro Yaya Alain ◽  
Awadji Jospin Morand ◽  
Botezatu Dediu Andreea ◽  
Olaye Théophile ◽  
Agbangnan Dossa Cokou Pascal ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Total Flavonoids ◽  
Skin Infections ◽  
Total Phenols ◽  
Anti Inflammatory

In the last decades, it has been intensively studied natural alternatives to synthetic products. Chassalia kolly is one of the medicinal plants used traditionally for treatment of skin infections. This study aimed at identifying the diversity secondary metabolites, to investigate the antioxidant and anti-inflammatory activities of ethanolic extract of Chassalia kolly leaves. Total phenols were determined by Folin Ciocalteu method. The aluminum trichloride method has been used to quantify total flavonoids, while the determination of condensed tannins was carried out by the hydrochloric vanillin method. The antioxidant capacity was evaluated by 2, 2-diphenyl-l-picrylhydrazyl (DPPH), 2, 2'-Azino-bis- (3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) and phosphomolybdenum methods. In vitro anti-inflammatory activity of ethanolic extract of Chassalia kolly leaves was evaluated by method membrane stabilization. In this results, Chassalia kolly leaves contain flavonoid, mucilages, anthocyans, sterols and terpenes, saponosides, alkaloids and reducing compounds. The contents of total phenols, total flavonoids and total tannins are respectively (20.41±2.11) µgGAE/mgDM; (30.29±2.18) µgQE/mgDM and (1.211±0.122)µgCE/mgDM. The results obtained during this work allowed us to assert that all extracts of the studied plant have very good antioxidant properties (IC50=0.05µg/µL). The ethanolic extract of the leaves of Chassalia kolly showed an anti-inflammatory activity more interesting than aspirin which is the reference compound used in this study.

scholarly journals PHYTOCHEMICAL ANALYSIS AND SUPPRESSION OF INFLAMMATORY TARGETS BY ADATHODA VASICA

2018 ◽  
Vol 11 (5) ◽  
pp. 162
Author(s):  
Amala Reddy ◽  
Sujatha Sundaresan
Keyword(s):  
Methanolic Extract ◽  
Maximum Yield ◽  
Foot Ulcer ◽  
Significant Inhibition ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Membrane Stabilization ◽  
Standard Drug ◽  
Anti Inflammatory

 Objectives: A persistent and chronic inflammation may act as an underlying definition for many of the long-term illness such as diabetic foot ulcer, Alzheimer’s disease and rheumatoid arthritis. Folklore medicinal plants provide immense health benefits with new remedies. Active compounds from plants containing alkaloids and phenolic compounds (flavonoids and tannins) possess antioxidant and anti-inflammatory activity. The leaves of Adhatoda vasica are most known for its effectiveness in treating respiratory diseases such as asthma, chronic bronchitis, and other respiratory conditions. In this report, we investigated the possible action of A. vasica against inflammation.Methods: Solvents with varying polarity (Hexane, dichloromethane, ethyl acetate, and methanol) were used for the sequential extraction of leaves of A. vasica Linn (Family: Acanthaceae). The anti‐inflammatory activity was assessed using simple in vitro methods. Phenols, flavonoids, phytosterols, saponins, alkaloids, and tannins were analyzed qualitatively using standard protocols. Anti‐inflammatory activity was evaluated using albumin denaturation assay, membrane stabilization assay and proteinase inhibitory activity. Aspirin (diclofenac) was used as a standard drug for the study of anti-inflammatory activity.Results: Our results substantiated that methanolic extract of A. vasica gave a maximum yield of 12.5% and apparently all bioactive phytochemical constituents were at a higher concentration in the methanolic extract. Methanolic extract of A. vasica showed a significant inhibition of the heat-induced albumin denaturation, red blood cells membrane stabilization, and proteinase inhibitory effects with 86±1.46, 88±1.24, and 96±0.93% for 1000 μg/ml, respectively.Conclusion: The above results delineate that the bioactive components were extracted using high polar solvent are mostly flavonoids and related polyphenols. These compounds may be the active constituents to render the anti-inflammatory activity to A. vasica.

scholarly journals Intestinal Anti-Inflammatory Activity ofBaccharis dracunculifoliain the Trinitrobenzenesulphonic Acid Model of Rat Colitis

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Sílvia Helena Cestari ◽  
Jairo Kennup Bastos ◽  
Luiz Claudio Di Stasi
Keyword(s):  
Lipid Peroxidation ◽  
Plant Extract ◽  
Inflammatory Activity ◽  
Glutathione Depletion ◽  
Phytochemical Analysis ◽  
Coumaric Acid ◽  
Brain Membrane ◽  
Anti Inflammatory ◽  
Improve Health

Baccharis dracunculifoliaDC (Asteraceae) is a Brazilian medicinal plant popularly used for its antiulcer and anti-inflammatory properties. This plant is the main botanical source of Brazilian green propolis, a natural product incorporated into food and beverages to improve health. The present study aimed to investigate the chemical profile and intestinal anti-inflammatory activity ofB. dracunculifoliaextract on experimental ulcerative colitis induced by trinitrobenzenosulfonic acid (TNBS). Colonic damage was evaluated macroscopically and biochemically through its evaluation of glutathione content and its myeloperoxidase (MPO) and alkaline phosphatase activities. Additionalin vitroexperiments were performed in order to test the antioxidant activity by inhibition of induced lipid peroxidation in the rat brain membrane. Phytochemical analysis was performed by HPLC using authentic standards. The administration of plant extract (5 and 50 mg kg−1) significantly attenuated the colonic damage induced by TNBS as evidenced both macroscopically and biochemically. This beneficial effect can be associated with an improvement in the colonic oxidative status, since plant extract prevented glutathione depletion, inhibited lipid peroxidation and reduced MPO activity. Caffeic acid,p-coumaric acid, aromadendrin-4-O-methyl ether, 3-prenyl-p-coumaric acid, 3,5-diprenyl-p-coumaric acid and baccharin were detected in the plant extract.

scholarly journals Design, Synthesis and Biological Screening of Novel 1,5-Diphenyl-3-(4-(trifluoromethyl)phenyl)-2-pyrazoline Derivatives

2020 ◽  
Vol 67 (4) ◽  
pp. 1139-1147
Author(s):  
Fatih Tok ◽  
Bedia Koçyiğit-Kaymakçıoğlu
Keyword(s):  
Inflammatory Activity ◽  
Antidiabetic Activity ◽  
Spectroscopic Methods ◽  
Design Synthesis ◽  
Reference Drug ◽  
Ic50 Values ◽  
Adme Properties ◽  
Anti Inflammatory ◽  
In Vitro Antioxidant Activity

1-Phenyl-5-substituted-3-(4-(trifluoromethyl)phenyl)-4,5-dihydro-1H-pyrazole derivatives were synthesized from chalcone derivatives. The structures of compounds were characterized by IR, 1H NMR spectroscopic methods and elemental analysis. All compounds were evaluated for their in vitro antioxidant activity using DPPH and ABTS methods, anti-inflammatory activity using lipoxygenase inhibitory method and antidiabetic activity using the α-glucosidase inhibitory method. Especially, pyrazoline derivatives exhibited stronger anti-inflammatory activity than the reference drug indomethacin (IC50: 50.45 μM) and their IC50 values were in the range of 0.68 and 4.45 μM. In addition, the ADME properties of all chalcone and pyrazoline derivatives were calculated by Lipinski’s and Veber’s rules.

scholarly journals EVALUATION OF ANTI-INFLAMMATORY AND ANTIDIABETIC ACTIVITY OF SIMAROUBA GLAUCA BARK EXTRACT: AN IN VITRO STUDY

2020 ◽  
pp. 74-78
Author(s):  
ALEESHA R ◽  
BHARAT MISHRA
Keyword(s):  
In Vitro Study ◽  
Inflammatory Activity ◽  
Antidiabetic Activity ◽  
Bark Extract ◽  
Potential Candidate ◽  
Bioactive Constituents ◽  
Ic50 Value ◽  
Anti Inflammatory ◽  
Simarouba Glauca

Objectives: The present study was envisaged to identify the effect of anti-inflammatory and antidiabetic activity for methanolic bark extracts of Simarouba glauca. Methods: The present study design was to evaluate the in vitro anti-inflammatory and in vitro antidiabetic activity of S. glauca methanolic bark extract. To examine the antidiabetic activity, the samples were studied for their effect on inhibition of alpha-amylase and glucose transport across the dialysis membrane. In vitro anti-inflammatory activity was evaluated using albumin denaturation assay and membrane stabilization method. Results: Our current results indicate that the various bioactive constituents detected in S. glauca may be responsible for its in vitro antidiabetic and anti-inflammatory effects. The ability of plant extract on anti-inflammatory activity showed that it was effective in inhibiting heat-induced albumin denaturation with an IC50 value of test and standard was found to be 46.42 μg/ml and 24.09 μg/ml. In addition to this, heat-induced hemolysis was also performed. The IC50 values of the test and standard were found to be 43.51 μg/ml and 21.41 μg/ml, respectively. The percentage inhibition of the test sample varied from the concentration range of 75 to 100 μg/ml. The IC50 value of the test and standard was found to be 19.08 μg/ml and 9.08 μg/ml, respectively. Conclusion: The findings of the present study concluded that S. glauca bark has the potential to treat diabetes and a novel natural anti-inflammatory agent as a good source. Thus, S. glauca may be a potential candidate for the development of future antidiabetic and anti-inflammatory compounds. However, still further studies and standardization of the plant research may be required to develop them as medicine.

scholarly journals Design, Synthesis, in Vitro Antioxidant, Anti-Inflammatory and Antidiabetic Evaluation of New N-Substitutedbenzylidene-5-(4-Formylphenyl)-(3-Hydroxyphenyl)-4,5-Dihydropyrazole-1-Carbothioamide Derivatives

2020 ◽  
Author(s):  
Sucheta Singh Singh ◽  
sumit Tehlan Tehlan ◽  
prabhakar kumar verma
Keyword(s):  
Antioxidant Activity ◽  
Heterocyclic Compounds ◽  
Inflammatory Activity ◽  
Antidiabetic Activity ◽  
Design Synthesis ◽  
Standard Drug ◽  
Wide Range ◽  
Anti Inflammatory ◽  
Clinical Potential

Abstract A series of N-substitutedbenzylidene-5-(4-formylphenyl)-(3-hydroxyphenyl)-4,5-dihydropyrazole-1-carbothioamide derivatives was designed, synthesized and examined for their therapeutical potential against prooxidant (oxidative stress), inflammation and diabetes. Biological results showed antioxidant activity with IC50 value 37.68 mol/L, anti-inflammatory activity with IC50 value 26.40 mol/L and antidiabetic activity with IC50 value 17.12 mol/L. The results of antioxidant activity showed that compounds Y9 and Y17 exhibited excellent antioxidant activity with IC50 values 17.43 mol/L and 18.98 mol/L, results of anti-inflammatory activity showed that compounds Y2, Y3 and Y7 exhibited excellent anti-inflammatory activity with IC50 values 23.23 mol/L, 22.09 mol/L and 19.05 mol/L respectively and results of antidiabetic activity showed that compounds Y1, Y5and Y6 exhibited excellent antidiabetic activity with IC50 values 17.08 mol/L, 8.36 mol/L and 13.50 mol/L. When compared with ascorbic acid, aspirin and acarbose as standard drug respectively. Heterocyclic compounds have diversity in their structure which makes them broad and economical therapeutic agents. Pyrazole is a five membered ring containing three carbon and two neighboring nitrogen atoms. Pyrazole and its derivatives have various biological as well as clinical potential thus considered for further research. Due to wide range of therapeutical activities pyrazole makes interest among researcher to explore it further for more activities. Pyrazole is present in various biological moieties eg. antimicrobial, antidiabetic, anti-inflammatory, antioxidant, antiviral, anticonvulsant, anticancer, anti-HIVand anti-tuberculosis agents.

scholarly journals In-vitro Anti-Inflammatory activity of Digera muricata Extracts

2020 ◽  
Vol 11 (4) ◽  
pp. 5748-5751
Author(s):  
Balaji Subramaniyan R ◽  
Jaikumar S ◽  
Sekkizhar M
Keyword(s):  
In Vitro Models ◽  
Inflammatory Activity ◽  
Antidiabetic Activity ◽  
Ethical Problems ◽  
Synthetic Drugs ◽  
Tissue Degeneration ◽  
Alternative Means ◽  
Anti Inflammatory ◽  
Work Done

Inflammation is a process that is generally termed for pain and redness, which includes swelling. But it is defined as a heterogeneous group of processes which result in the pain and increase in vascular circulation and denaturation of protein that results in tissue degeneration. There are many synthetic drugs available to treat and relieve the inflammation. They are potent and have many side effects too. So there is an increased search for alternative means of treatment option. Thereby there was a high focus on the medicinal plants and herbs. Digera muricate, which is used to treat many diseases like analgesic activity, antidiabetic activity and anticancer activity too, this plant has proven to possess the antioxidant potential also. So there is no enough work done to verify the inflammatory potential of the plant. This was the primary consideration for this research to select the plant to prove it has anti-inflammatory potential. Having in the account that the ethical problems in the usage of lab animals, the anti-inflammatory potential of the plant was evaluated in-vitro using enzyme inhibition assays. The in-vitro models of the inflammatory activity are used for testing the anti-inflammatory activity, and the results were not different from invivo models. The extracts showed a dose based rise in the action, and the plant process a better anti-inflammatory activity than the standard drugs.

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