scholarly journals Phytochemical Study and Antioxidative Property of Ethanolic Extract from Termitomyces clypeatus

Author(s):  
Payel Mitra ◽  
Narayan Mandal ◽  
Anirban Roy ◽  
Krishnendu Acharya
2020 ◽  
Vol 15 (2) ◽  
pp. 41-57
Author(s):  
Fatma Acheuk ◽  
◽  
Kemais Abdellaoui ◽  
Wassima Lakhdari ◽  
Nora Chahbar ◽  
...  

The insecticidal potential of the Saharan plant Cotula cinerea, was evaluated on two insect species namely Aphis fabae and Tribolium castaneum by topical application (contact toxicity) and repellency test. A crude ethanolic extract of aerial part of the plant was prepared and tested in the laboratory on adults of both species. For contact toxicity, five doses were tested on each of the two species 1.56, 3.12, 6.25, 12.5 and 25 mg/ml for A. fabae and 25, 50, 250, 350 and 500 µg/insect for T. castaneum. The repellency of the extract was studied at the dose 500 μg/insect for T. castaneum and 25 µg/ml for A. fabae. Results showed that the repellency of the extract increased with exposure time and the highest rates were observed after 4 h of exposure (72.33 ± 22% for T. castaneum and 87 ± 3.6% for A. fabae). For insecticidal activity, at the highest doses (25 mg/ml and 500 µg/ml), 100% mortality is obtained 72 h after treatment for A. fabae and after 48 h for T. castaneum. The extract of this plant was found to be more toxic against T. castaneum adults. LD50 calculated 24 h after treatment for the two species is estimated at 1.7 mg/ml for A. fabae and at 30.3 µg/insect for T. castaneum. The extract of this plant inhibited the activity of acetylcholinesterase (AChE) in both insect species. This result suggests that this plant has a neurotoxic effect on A. fabae and T. castaneum. The results of phytochemical study showed that the plant is mainly rich in flavonoids, gallic tannins, alkaloids, saponosides and glucosides. The insecticidal effect obtained in this study could be due to the synergetic action of all constituents of the extract. Results suggest the possibility of using the extracts of this plant in integrated pest management to replace the chemical insecticides.


Pathogens ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 889
Author(s):  
Francisco Flávio Vieira de Assis ◽  
Nazaré Carneiro da Silva ◽  
Waldiney Pires Moraes ◽  
Lauro Euclides Soares Barata ◽  
Antonio Humberto Hamad Minervino

Cyperus articulatus L. is popularly known as priprioca. Its rhizomes are used as a medicine in the treatment of malaria in traditional medicine. Since priprioca oil is extracted for commercial purpose, we evaluated if the components from the priprioca residue can be a source of antiplasmodial active molecules. This study aimed to determine the in vitro antiplasmodial and cytotoxicity activities of the ethanolic extract of C. articulatus as an in vitro antiplasmodial agent. From the solid residue of the plant rhizomes, 40 g samples were removed and subjected to hot extraction using a Soxhlet extractor. The in vitro antiplasmodial activity was determined using the W2 and 3D7 strains of P. falciparum. The phytochemical study identified the following main compounds: corymbolone (14.25%), cyclocolorenone (9.75%), and cadalene (8.36%). The extract exhibited moderate IC50 (inhibitory concentration) against the two strains of P. falciparum: 1.21 ± 0.01 against the W2 strain and 1.10 ± 0.06 µg/mL against the 3D7 strain. Our results show the therapeutic potential of priprioca residue as a low-cost antiplasmodial agent.


2019 ◽  
Vol 12 (9) ◽  
pp. 4287
Author(s):  
Payel Mitra ◽  
Arun Kumar Dutta ◽  
Narayan Chandra Mandal ◽  
Krishnendu Acharya

Author(s):  
Hasna.T ◽  
Vivek.P ◽  
Manojkumar.N

The ancient wisdom in Ayurveda medicine is still not exhaustively explored. Multiple exotic plants existent in India which are not described in classical literature of Ayurveda are commonly referred to as Anukta Dravya (undocumented). Costus pictus D.Don is such a plant, recently introduced in India from Mexico, which is used for renal disorders there. In India, it is used in Diabetes mellitus. This is proven for antidiabetic, antioxidant, anticancerous, antimicrobial and diuretic actions. It is an easily propagated, palatable and cost effective plant. Identification through pharmacognostical and phytochemical studies is essential for the standardization of any plant. The green leaf is narrowly elliptic with 10 to 25cm length and 2.5 to 6cm width. Microscopy revealed presence of vascular bundles, unicellular trichomes in upper epidermis, thin walled parenchyma cells in ground tissue and layers of parenchymatous hypodermis. Powder microscopy showed presence of epidermal parenchyma cells with underlying chlorenchyma group of fibers and fibro vascular bundles.TLC photo documentation revealed presence of many phytoconstituents with different Rf values. Densitometric scan showed many peaks, 10 at 254nm, 11 at 366nm, 6 at 520nm and 9 at 620 nm after derivatisation. Moisture content was 20%. The percentage of total ash, acid insoluble ash, and water insoluble ash was determined. The water soluble extractive value was 22.57 which is highest among all the extracts. The results indicated the presence of alkaloid, steroids, tannins, flavanoids, phenol, carbohydrate and resin in ethanolic extract and steroid, flavanoids, phenols, saponins, tannins and glycosides in water extract.


2021 ◽  
Vol 9 (11) ◽  
pp. 1143-1150
Author(s):  
Yeo Sounta Oumar ◽  
◽  
Monyn Ebalah Delphine ◽  
Silue Kalamourou ◽  
Mawa Traore ◽  
...  

The aim of this work is to study phytochemicals and evaluate the antibacterial activity of aqueous and ethanolic extracts of Myrianthus holstii bark on three reference and five clinical strains derived from biological products. The results obtained show that the gives the best extraction yield. Chemical screening revealed the presence of polyphenols, alkaloids, flavonoids, saponosides, quinones, anthocyanins, tannins, terpenoids and sterols in both extracts. The results obtained show that the strains tested have a variable sensitivity for the two extracts and their concentrations. Diffusion and dilution methods on Muller-Hinton were used to evaluate the antibacterial activity of the extracts. The diameters of the inhibition zones are between 8 and 16 mm for the ethanolic extract starting from 25 mg/mL and between 8 and 12 mm for the aqueous extract at 50 mg/mL. The results revealed that these extracts have a dose-dependent antibacterial activity on the bacterial strains used. However, the 70% ethanolic extract has a better antibacterial potential on the strains compared with the aqueous extract, namely on S. aureus (CMI=3.12 mg/mL), E. coli ATCC (CMI=12.5 mg/mL) and S. aureus Meti-R (CMI=12.5 mg/mL). Also, this extract is bactericidal on all strains studied and its MIC ranges from 3.12 to 100 mg/mL after 24 and 48 hours of incubation. This study showed that extracts of Myrianthus holstii could be used in the treatment of infectious diseases.


1970 ◽  
Vol 4 (1) ◽  
pp. 64-68 ◽  
Author(s):  
Utpal Kumar Karmakar ◽  
Dyuti Ghosh ◽  
Samir Kumar Sadhu

The ethanolic extract of leaves and stem of Vallaris solanacea (Roth) Kuntze (Family: Apocynaceae) was screened for its analgesic, cytotoxic and antioxidant activities. Phytochemical analysis of the extract indicated the presence of Reducing Sugar, Tannins, Saponins, Gums, Steroids, Alkaloids, and Glycosides. The ethanolic extract showed statistically significant analgesic activity (p<0.005) in acetic acid induced writhing inhibition in mice at the dose of 500mg/kg body weight and also showed mild effect at the doses of 250mg/kg body weight. In the brine shrimp lethality test, the extract showed cytotoxicity with LC50 80 μg/ml and LC90 320 μg/ml. In the qualitative antioxi-dant assay using DPPH (1, 1-diphenyl-2-picryl hydrazyl) the extract showed free radical scavenging properties. These primary findings suggest that the extract might possess some chemical constituents that are responsible for analgesic, cytotoxic and antioxidant activities. Key words: Vallaris solanacea (Roth) Kuntze; phytochemical study; analgesic activity; cytotoxic activity; antioxi-dant activity. DOI: http://dx.doi.org/10.3329/sjps.v4i1.8869 SJPS 2011; 4(1): 64-68


Author(s):  
Ankita Prakash Kore ◽  
Nilofar Shahnwaj Naikwade ◽  
Padma Laxmikant Ladda ◽  
Tabbasum Shabbirahmad Shikalgar ◽  
Shirish Sidgonda Patil

Cajanus cajan (L) Millsp. is the perennial plant belongs to family Fabaceae, commonly called as Pigeon pea plant. The presence of phytoconstituents like flavonoids, the flavanone (substituted) from Cajanus cajan (L) Millsp. have in vitro neuroactive property. This flavanone named as pinostrobin helps to inhibit voltage – gated sodium channels. Because of its bioactive phytoconstituents it may act as antiepileptic drug. To avoid problems like ADR herbal plant might be alternative to treat epilepsy. The current study was therefore carried out to evaluate antiepileptic activity of Ethanolic extract of leaves of Cajanus cajan in rodents. The effect of ELECC in MES-induced convulsions in rat and PTZ-induced convulsion in mice was evaluated using doses 100 mg/kg and 200 mg/kg for 7 days. Phenytoin (25 mg/kg), Diazepam (4 mg/kg) was used as standard drug for respective model. Depending on the model, outcome measures were abolishment of Hind Limb Tonic Extensor phase in MES-induced convulsion in rat and onset of latency, recovery or death in PTZ-induced convulsion in mice as well as biochemical estimation of amino acid neurotransmitter (GABA, Glutamate) were evaluated. The ELECC at doses 100 and 200 mg/kg significantly delayed the HLTE phase in MES-induced convulsions in rat whereas, significantly increased onset of latency in PTZ-induced convulsion in mice. It also showed significant (p>0.0001) effect on the level of GABA and Glutamate in dose dependent manner in both models. The phytochemical study of C. cajan showed the presence of Glycosides, Flavonoids, Flavonones, Steroids, Tannins, Fixed oil, Fatty acids and Proteins. As the flavonoids present in C. cajan may contribute to the anticonvulsant activity of the plant. Therefore, the presence of such compounds in the extract may be responsible for the anticonvulsant effect. Therefore, present study validates its anticonvulsant activity. Further, research is required to elucidate its specific mechanism of action and isolation of responsible active principles.


INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (11) ◽  
pp. 15-24
Author(s):  
V.P Londhe ◽  
◽  
S. S Nipate ◽  
A.H. Tiwari ◽  
P.D. Chaudhari

Ficus racemosa (Family: Moracae) is a prominent plant in India and it was embedded in traditional medicinal texts of India as a wound healer. Hence to study its wound healing assets, the ethanolic leaves extract was studied on animals. Excision and incision wound models were used to study wound healing activity. For both models, extract was given in the form of ointment (5% and 10 % w/w). In the excision model, all extract treated animals showed significant (P<0.01) increase in percentage wound contraction. The incision wound model showed significant (P<0.01) increase in breaking strength when compared to control. In biochemical parameter, hydroxyproline level was significantly (P<0.01) increased in all drugs treated groups as compared to control in the excision wound model. Histopathological study also showed good regeneration of skin compared to control. Phytochemical study of the leaves showed presence of flovonoids and tannins which might be the key constituents for the wound healing activity.


2019 ◽  
Vol 10 (2) ◽  
pp. 1470-1473
Author(s):  
Jannathul firdous ◽  
Karpagam T ◽  
Varalakshmi ◽  
Sugunabai J ◽  
Gomathi S ◽  
...  

Medicinal plant Aloe vera are reported to have anti-cancer properties. In the present work, a phytochemical study in Aloe vera was evaluated qualitatively and further analysed using gas chromatography. Aloe vera leaves were washed, shade dried and homogenized to get a filtrate which was extracted in ethanol using Soxhlet apparatus. The ethanolic extract was subjected to various phytochemical tests and also evaluated using gas chromatography. As a result, the qualitative phytochemical analysis of A.vera ethanolic leaves extracts confirmed the presence of alkaloids, flavonoids, carbohydrates, proteins, saponins, phenols, terpenoids and phytosterols. Gas chromatographic analysis of A.vera ethanolic leaves extracts showed all these eight compounds. All these compounds are of pharmacological importance as they possess properties such as anti-cancer, anti-diabetic, analgesic, antibacterial, and antifungal activity. Future work is essential to explore its therapeutic applications.


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