scholarly journals Purification and characterization of an iron-activated alkaline phosphatase produced by Rhizopus microsporus var. microsporus under submerged fermentation using rye flour

2020 ◽  
Author(s):  
Ornela Pedro Henrique de Oliveira ◽  
Guimarães Luis Henrique Souza
Keyword(s):  
Alkaline Phosphatase ◽  
Submerged Fermentation ◽  
Purification And Characterization ◽  
Rhizopus Microsporus ◽  
Rye Flour

Purification and Characterization of a Chymosin from Rhizopus microsporus var. rhizopodiformis

2014 ◽  
Vol 174 (1) ◽  
pp. 174-185 ◽  
Author(s):  
Qian Sun ◽  
Xi-Ping Wang ◽  
Qiao-Juan Yan ◽  
Wei Chen ◽  
Zheng-Qiang Jiang
Keyword(s):  
Purification And Characterization ◽  
Rhizopus Microsporus

Purification and characterization of alkaline phosphatase from the gut of sea cucumber Stichopus japonicus

2013 ◽  
Vol 79 (3) ◽  
pp. 477-485 ◽  
Author(s):  
Hai-Tao Wu ◽  
Dong-Mei Li ◽  
Bei-Wei Zhu ◽  
Jing-Heng Cheng ◽  
Jin-Jian Sun ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Sea Cucumber ◽  
Purification And Characterization ◽  
Stichopus Japonicus

scholarly journals Revealing enzyme functional architecture via high-throughput microfluidic enzyme kinetics

Science ◽  
2021 ◽  
Vol 373 (6553) ◽  
pp. eabf8761
Author(s):  
C. J. Markin ◽  
D. A. Mokhtari ◽  
F. Sunden ◽  
M. J. Appel ◽  
E. Akiva ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Kinetics ◽  
High Throughput ◽  
Active Site ◽  
Molecular Mechanisms ◽  
Extensive Investigation ◽  
Purification And Characterization ◽  
Multiple Substrates ◽  
Catalytic Effects

Systematic and extensive investigation of enzymes is needed to understand their extraordinary efficiency and meet current challenges in medicine and engineering. We present HT-MEK (High-Throughput Microfluidic Enzyme Kinetics), a microfluidic platform for high-throughput expression, purification, and characterization of more than 1500 enzyme variants per experiment. For 1036 mutants of the alkaline phosphatase PafA (phosphate-irrepressible alkaline phosphatase of Flavobacterium), we performed more than 670,000 reactions and determined more than 5000 kinetic and physical constants for multiple substrates and inhibitors. We uncovered extensive kinetic partitioning to a misfolded state and isolated catalytic effects, revealing spatially contiguous regions of residues linked to particular aspects of function. Regions included active-site proximal residues but extended to the enzyme surface, providing a map of underlying architecture not possible to derive from existing approaches. HT-MEK has applications that range from understanding molecular mechanisms to medicine, engineering, and design.

scholarly journals Purification and characterization of alkaline phosphatase of Bacteroides gingivalis 381.

1990 ◽  
Vol 58 (9) ◽  
pp. 2882-2887 ◽  
Author(s):  
Y Yamashita ◽  
K Toyoshima ◽  
M Yamazaki ◽  
N Hanada ◽  
T Takehara
Keyword(s):  
Alkaline Phosphatase ◽  
Purification And Characterization ◽  
Bacteroides Gingivalis

scholarly journals Purification and characterization of alkaline phosphatase from lactic acid bacteria

RSC Advances ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 354-360 ◽  
Author(s):  
Yu-Hao Chu ◽  
Xin-Xin Yu ◽  
Xing Jin ◽  
Yu-Tang Wang ◽  
Duo-Jia Zhao ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Organophosphorus Pesticides ◽  
Purification And Characterization

Alkaline phosphatase (ALP) excreted from lactic acid bacteria (LAB) showed the ability to degrade organophosphorus pesticides.

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