scholarly journals Cloning, expression and purification of resistance gene analogue RGPM 301 from pearl millet in Escherichia coli

2016 ◽  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Pearl Millet ◽  
Expression And Purification ◽  
Resistance Gene Analogue

Isolation and characterisation of a NBS-LRR resistance gene analogue from pearl millet

2011 ◽  
Vol 44 (10) ◽  
pp. 1014-1024 ◽  
Author(s):  
P. Ranjini ◽  
S. Shailasree ◽  
K. Ramachandra Kini ◽  
H. Shekar Shetty
Keyword(s):  
Resistance Gene ◽  
Pearl Millet ◽  
Resistance Gene Analogue

Expression and Purification of Bioactive High-Purity Recombinant Mouse SPP1 in Escherichia coli

2014 ◽  
Vol 173 (2) ◽  
pp. 421-432 ◽  
Author(s):  
Yunsheng Yuan ◽  
Xiyuan Zhang ◽  
Shunyan Weng ◽  
Wen Guan ◽  
Di Xiang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Purity ◽  
Expression And Purification ◽  
Recombinant Mouse

scholarly journals Characterization of the 6'-N-aminoglycoside acetyltransferase gene aac(6')-Im [corrected] associated with a sulI-type integron.

1997 ◽  
Vol 41 (2) ◽  
pp. 314-318 ◽  
Author(s):  
E Hannecart-Pokorni ◽  
F Depuydt ◽  
L de wit ◽  
E van Bossuyt ◽  
J Content ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Amino Acid ◽  
Resistance Gene ◽  
Citrobacter Freundii ◽  
Gram Negative ◽  
Gene Environment ◽  
Insert Region ◽  
Klebsiella Spp

The amikacin resistance gene aac(6')-Im [corrected] from Citrobacter freundii Cf155 encoding an aminoglycoside 6'-N-acetyltransferase was characterized. The gene was identified as a coding sequence of 521 bp located down-stream from the 5' conserved segment of an integron. The sequence of this aac(6')-Im [corrected] gene corresponded to a protein of 173 amino acids which possessed 64.2% identity in a 165-amino-acid overlap with the aac(6')-Ia gene product (F.C. Tenover, D. Filpula, K.L. Phillips, and J. J. Plorde, J. Bacteriol. 170:471-473, 1988). By using PCR, the aac(6')-Im [corrected] gene could be detected in 8 of 86 gram-negative clinical isolates from two Belgian hospitals, including isolates of Citrobacter, Klebsiella spp., and Escherichia coli. PCR mapping of the aac(6')-Im [corrected] gene environment in these isolates indicated that the gene was located within a sulI-type integron; the insert region is 1,700 bases long and includes two genes cassettes, the second being ant (3")-Ib.

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