scholarly journals Humoral immune response in cattle and buffaloes vaccinated against Pasteurella multocida B: 2 with reference to levels of B Lymphocytes and IL-2R+

2014 ◽  
Keyword(s):  
Immune Response ◽  
B Lymphocytes ◽  
Humoral Immune Response ◽  
Pasteurella Multocida ◽  
Humoral Immune

Some immunological studies on a new vaccine derived frombacteriophage lysate of Pasteurella multocida in mice

2017 ◽  
Author(s):  
Hari Mohan Saxena ◽  
Yanglem Pushpa ◽  
Sabia Qureshi
Keyword(s):  
Immune Response ◽  
Serum Protein ◽  
Humoral Immune Response ◽  
Protein Concentration ◽  
Pasteurella Multocida ◽  
Phage Lysate ◽  
Serum Globulin ◽  
Humoral Immune ◽  
Serum Igg Levels ◽  
Serum Protein Concentration

The study was undertaken to evaluate the humoral immune response in mice induced by a novel phage lysate vaccine against Hemorrhagic Septicemia developed from Pasteurella multocida grown under iron-restricted conditions and lysed by a bacteriophage. Two groups of mice were immunized with lysate vaccine (LV) and conventional killed HS vaccine (CV) and blood was collected at various days post – immunization (DPI). The serum protein concentration in lysate vaccinated mice (4.675±0.223) was significantly higher (p less than 0.05) than the conventional vaccinated mice (4.100±0.282) at 150 DPI. The serum globulin levels at 90 DPI and 180 DPI in LV mice (1.330±0.071 and 0.650±0.100) were significantly (p less than 0.01) higher than the CV mice (0.850±0.084 and 0.366±0.098). The serum IgG levels at 150 DPI and 180 DPI in LV (0.564±0.188 and 0.485±0121) mice were significantly higher (p less than 0.01) than the CV mice (0.178±0.039 and 0.121±0.026). Although our results in mice are promising, further studies involving bovines are needed.

scholarly journals Missing Links in Antibody Assembly Control

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Tiziana Anelli ◽  
Eelco van Anken
Keyword(s):  
Immune Response ◽  
B Cell ◽  
B Lymphocytes ◽  
Humoral Immune Response ◽  
Disulfide Bonds ◽  
Cell Receptor ◽  
Immunoglobulin M ◽  
Dependent Manner ◽  
Humoral Immune ◽  
Secretory Igm

Fidelity of the humoral immune response requires that quiescent B lymphocytes display membrane bound immunoglobulin M (IgM) on B lymphocytes surface as part of the B cell receptor, whose function is to recognize an antigen. At the same time B lymphocytes should not secrete IgM until recognition of the antigen has occurred. The heavy chains of the secretory IgM have a C-terminal tail with a cysteine instead of a membrane anchor, which serves to covalently link the IgM subunits by disulfide bonds to form “pentamers” or “hexamers.” By virtue of the same cysteine, unassembled secretory IgM subunits are recognized and retained (via mixed disulfide bonds) by members of the protein disulfide isomerase family, in particular ERp44. This so-called “thiol-mediated retention” bars assembly intermediates from prematurely leaving the cell and thereby exerts quality control on the humoral immune response. In this essay we discuss recent findings on how ERp44 governs such assembly control in a pH-dependent manner, shuttling between the cisGolgi and endoplasmic reticulum, and finally on how pERp1/MZB1, possibly as a co-chaperone of GRP94, may help to overrule the thiol-mediated retention in the activated B cell to give way to antibody secretion.

Tetraspanins in the humoral immune response

2011 ◽  
Vol 39 (2) ◽  
pp. 512-517 ◽  
Author(s):  
Annemiek B. van Spriel
Keyword(s):  
Immune Response ◽  
B Cell ◽  
B Lymphocytes ◽  
Humoral Immune Response ◽  
Cell Biology ◽  
Transmembrane Proteins ◽  
Receptor Activation ◽  
Ample Evidence ◽  
Humoral Immune ◽  
Mhc Molecules

The tetraspanins represent a large superfamily of four-transmembrane proteins that are expressed on all nucleated cells. Tetraspanins play a prominent role in the organization of the plasma membrane by co-ordinating the spatial localization of transmembrane proteins and signalling molecules into ‘tetraspanin microdomains’. In immune cells, tetraspanins interact with key leucocyte receptors [including MHC molecules, integrins, CD4/CD8 and the BCR (B-cell receptor) complex] and as such can modulate leucocyte receptor activation and downstream signalling pathways. There is now ample evidence that tetraspanins on B-lymphocytes are important in controlling antibody production. The tetraspanin CD81 interacts with the BCR complex and is critical for CD19 expression and IgG production, whereas the tetraspanin CD37 inhibits IgA production and is important for IgG production. By contrast, the tetraspanins CD9, Tssc6 and CD151 appear dispensable for humoral immune responses. Thus individual tetraspanin family members have specific functions in B-cell biology, which is evidenced by recent studies in tetraspanin-deficient mice and humans. The present review focuses on tetraspanins expressed by B-lymphocytes and discusses novel insights into the function of tetraspanins in the humoral immune response.

Potentiation of the humoral immune response elicited by a commercial vaccine against bovine respiratory disease by Enterococcus faecalis CECT7121

2018 ◽  
Vol 9 (4) ◽  
pp. 553-562 ◽  
Author(s):  
A.M. Díaz ◽  
B. Almozni ◽  
M.A. Molina ◽  
M.D. Sparo ◽  
M.A. Manghi ◽  
...  
Keyword(s):  
Immune Response ◽  
Respiratory Disease ◽  
Enterococcus Faecalis ◽  
Humoral Immune Response ◽  
Pasteurella Multocida ◽  
Bovine Respiratory Disease ◽  
Intragastric Administration ◽  
Adjuvant Effect ◽  
Humoral Immune ◽  
Commercial Vaccine

Vaccination against pathogens involved in bovine respiratory disease (BRD) is a useful tool to reduce the risk of this disease however, it has been observed that the commercially available vaccines only partially prevent the infections caused by Pasteurella multocida and Mannheimia haemolytica. Therefore, it is recommended to search for new adjuvant strategies to minimise the economic impact of this respiratory syndrome. A possibility to improve the conventional vaccine response is to modulate the immune system with probiotics, since there is accumulating evidence that certain immunomodulatory strains administered around the time of vaccination can potentiate the immune response. Considering veterinary vaccines are frequently tested in murine models, we have developed an immunisation schedule in BALB/c mice that allows us to study the immune response elicited by BRD vaccine. In order to evaluate a potential strategy to enhance vaccine efficacy, the adjuvant effect of Enterococcus faecalis CECT7121 on the murine specific humoral immune response elicited by a commercial vaccine against BRD was studied. Results indicate that the intragastric administration of E. faecalis CECT7121 was able to induce an increase in the specific antibody titres against the bacterial components of the BRD vaccines (P. multocida and M. haemolytica). The quality of the humoral immune response, in terms of antibody avidity, was also improved. Regarding the cellular immune response, although the BRD vaccination induced a low specific secretion of cytokines in the spleen cell culture supernatants, E. faecalis CECT7121-treated mice showed higher interferon-γ production than immunised control mice. Our results allowed us to conclude that the administration of E. faecalis CECT7121 could be employed as an adjuvant strategy to potentiate humoral immune responses.

Role of Yersinia pseudotuberculosis outer proteins (Yops) in murine humoral immune response

2009 ◽  
Vol 54 (3) ◽  
pp. 239-245 ◽  
Author(s):  
J. M. L. Maia ◽  
L. G. S. Monnazzi ◽  
B. M. M. Medeiros
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Yersinia Pseudotuberculosis ◽  
Humoral Immune

scholarly journals Diagnostic and analytical performance evaluation of ten commercial assays for detecting SARS-CoV-2 humoral immune response

2021 ◽  
pp. 113043
Author(s):  
Marnix Mylemans ◽  
Eveline Van Honacker ◽  
Louis Nevejan ◽  
Stefanie van den Bremt ◽  
Laura Hofman ◽  
...  
Keyword(s):  
Immune Response ◽  
Performance Evaluation ◽  
Humoral Immune Response ◽  
Analytical Performance ◽  
Humoral Immune
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