scholarly journals Urinary nucleic acids can be concentrated, dried on membranes and stored at room temperature in vacuum bags

2014 ◽  
Author(s):  
Fanshuang Zhang ◽  
Yuan Yuan ◽  
Jianqiang Wu ◽  
Youhe Gao
Keyword(s):  
Nucleic Acids ◽  
Large Scale ◽  
Room Temperature ◽  
Invasive Disease ◽  
The Body ◽  
Clinical Samples ◽  
Good Source ◽  
Disease Biomarkers ◽  
Non Invasive

Urine accumulates traces of changes that occur in the body and can potentially serve as a better biomarker source. Urinary nucleic acids are a promising class of non-invasive disease biomarkers. However, long-term frozen human urine samples are not a good source for the extraction of nucleic acids. In this paper, we demonstrate that urinary nucleic acids can be concentrated, dried on membranes and stored in vacuum bags at room temperature for several months. The amount of total RNA on the membranes after storage at room temperature for three months was unchanged. The levels of miR-16 and miR-21 exhibited no significant differences (P = 0.564, 0.386). This simple and economical method makes the large-scale storage of clinical samples of urinary nucleic acids possible.

scholarly journals Urinary nucleic acids can be concentrated, dried on membranes and stored at room temperature in vacuum bags

2014 ◽  
Author(s):  
Fanshuang Zhang ◽  
Yuan Yuan ◽  
Jianqiang Wu ◽  
Youhe Gao
Keyword(s):  
Nucleic Acids ◽  
Large Scale ◽  
Room Temperature ◽  
Invasive Disease ◽  
The Body ◽  
Clinical Samples ◽  
Good Source ◽  
Disease Biomarkers ◽  
Non Invasive

Urine accumulates traces of changes that occur in the body and can potentially serve as a better biomarker source. Urinary nucleic acids are a promising class of non-invasive disease biomarkers. However, long-term frozen human urine samples are not a good source for the extraction of nucleic acids. In this paper, we demonstrate that urinary nucleic acids can be concentrated, dried on membranes and stored in vacuum bags at room temperature for several months. The amount of total RNA on the membranes after storage at room temperature for three months was unchanged. The levels of miR-16 and miR-21 exhibited no significant differences (P = 0.564, 0.386). This simple and economical method makes the large-scale storage of clinical samples of urinary nucleic acids possible.

scholarly journals Catheter Ablation in Persistent AF, the Evolution towards a More Pragmatic Strategy

2021 ◽  
Vol 10 (18) ◽  
pp. 4060
Author(s):  
Louisa O’Neill ◽  
Jean-Yves Wielandts ◽  
Kris Gillis ◽  
Gabriela Hilfiker ◽  
Jean-Benoit Le Polain De Waroux ◽  
...  
Keyword(s):  
Catheter Ablation ◽  
Large Scale ◽  
The Body ◽  
Success Rates ◽  
Meaningful Improvement ◽  
Non Invasive ◽  
Technological Advances ◽  
Mapping Techniques ◽  
Catheter Technology

Atrial fibrillation (AF) is the most common cardiac arrhythmia worldwide and represents a heterogeneous disorder with a complex pathological basis. While significant technological advances have taken place over the last decade in the field of catheter ablation of AF, response to ablation varies and long-term success rates in those with persistent AF remain modest. Mechanistic studies have highlighted potentially different sustaining factors for AF in the persistent AF population with substrate-driven focal and re-entrant sources in the body of the atria identified on invasive and non-invasive mapping studies. Translation to clinical practice, however, remains challenging and the application of such mapping techniques to clinical ablation has yet to demonstrate a significant benefit beyond pulmonary vein isolation (PVI) alone in the persistent AF cohort. Recent advances in catheter and ablation technology have centered on improving the durability of ablation lesions at index procedure and although encouraging results have been demonstrated with early studies, large-scale trials are awaited. Further meaningful improvement in clinical outcomes in the persistent AF population requires ongoing advancement in the understanding of AF mechanisms, coupled with continuing progress in catheter technology capable of delivering durable transmural lesions.

scholarly journals Anti-meningococcal B vaccination in Italian adolescents: a cost-effective health opportunity

2020 ◽  
Vol 30 (Supplement_5) ◽  
Author(s):  
D Panatto ◽  
P Landa ◽  
D Amicizia ◽  
P L Lai ◽  
E Lecini ◽  
...  
Keyword(s):  
Large Scale ◽  
Public Health Problem ◽  
Vaccination Strategy ◽  
Cost Effective ◽  
Dose Schedule ◽  
Developed Countries ◽  
Invasive Disease ◽  
International Setting ◽  
The Impact

Abstract Background Invasive disease due to Neisseria meningitidis (Nm) is a serious public health problem even in developed countries, owing to its high lethality rate (8-15%) and the invalidating sequelae suffered by many (up to 60%) survivors. As the microorganism is transmitted via the airborne route, the only available weapon in the fight against Nm invasive disease is vaccination. Our aim was to carry out an HTA to evaluate the costs and benefits of anti-meningococcal B (MenB) vaccination with Trumenba® in adolescents in Italy, while also considering the impact of this new vaccination strategy on organizational and ethics aspects. Methods A lifetime Markov model was developed. MenB vaccination with the two-dose schedule of Trumenba® in adolescents was compared with 'non-vaccination'. Two perspectives were considered: the National Health Service (NHS) and society. Three disease phases were defined: acute, post-acute and long-term. Epidemiological, economic and health utilities data were taken from Italian and international literature. The analysis was conducted by means of Microsoft Excel 2010®. Results Our study indicated that vaccinating adolescents (11th year of life) with Trumenba® was cost-effective with an ICER = € 7,912/QALY from the NHS perspective and € 7,758/QALY from the perspective of society. Vaccinating adolescents reduces the number of cases of disease due to meningococcus B in one of the periods of highest incidence of the disease, resulting in significant economic and health savings. Conclusions This is the first study to evaluate the overall impact of free MenB vaccination in adolescents both in Italy and in the international setting. Although cases of invasive disease due to meningococcus B are few, if the overall impact of the disease is adequately considered, it becomes clear that including anti-meningococcal B vaccination into the immunization program for adolescents is strongly recommended from the health and economic standpoints. Key messages Free, large-scale MenB vaccination is key to strengthening the global fight against invasive meningococcal disease. Anti-meningococcal B vaccination in adolescents is a cost-effective health opportunity.

Adverse Effects of Long-Term Ice-Cold Water Drink on Rat Liver Function and Histology

2020 ◽  
Author(s):  
Said Abdul Ghafour Saeedy ◽  
Ahmad Faisal Faiz ◽  
Marjan Nikbakhtzadeh ◽  
Bagher Minaei Zangi ◽  
Mansoor Keshavarz
Keyword(s):  
Liver Function ◽  
Rat Liver ◽  
Room Temperature ◽  
Cold Water ◽  
The Body ◽  
Control Group ◽  
Male Wistar Rats ◽  
Temperature Water ◽  
Care Period

Drinking ice-cold water is prohibited in Avicenna’s “The Canon of Medicine” book in which he emphasized that ice-cold water drinking was hazardous for the body organs such as the liver. Little information can be found regarding the effects of ice-cold fluid drinks on liver and its probable sequels on this vital organ. Accordingly, we investigated the effects of long-term ice-cold water drink on the rat liver function and histology. Eighteen male Wistar rats, weighing 180±20 g, were randomly divided into three groups of six as two months ice-cold water drink, CW2M; three months ice-cold water drink, CW3M; and three months room temperature water drink; control group. Upon completion of the care period, a blood sample has been taken for liver enzymes and lipid profile assessment. Liver tissue has also been used for histological studies of H&E staining and microscopic examination. Histological findings showed hepatocellular micro-vesicle formation, necrosis and derangement of the cellular cords and infiltration of Kupffer cells in ice-cold water taken animals. Serum TGs, VLDL-C and ALP significantly increased with sound decrease in FBS and LDL-C in ice-cold water taken animals. It seems that long-term ice-cold water has deleterious functional and structural effects on the liver.

Lessons for Health Service: the Chernobyl Accident and the COVID-19 Pandemic

2020 ◽  
Vol 65 (3) ◽  
pp. 79-84
Author(s):  
A. Bushmanov ◽  
I. Galstyan ◽  
V. Solov'ev ◽  
M. Konchalovsky
Keyword(s):  
Chernobyl Accident ◽  
Large Scale ◽  
Medical Personnel ◽  
The Body ◽  
Key Factors ◽  
Vital Functions ◽  
Medical Institutions ◽  
Emergency Drugs ◽  
To Receive

Purpose: The article is devoted to the analysis of organizational measures to overcome the medical consequences of two large-scale events: the Chernobyl accident on 26.04.1986 and the COVID-19 pandemic. Results: A comparison of the causes, key factors, numbers affected and involved in these events persons, and the availability of the source of knowledge about the clinical picture, diagnosis, prevention and treatment of diseases, developing as a result of radiation exposure and the virus SARS-Cov-2. The article considers the availability of special medical institutions, the infectability of medical workers, the presence of long-term consequences for the health of victims and for the economy. Conclusions: In the development of such catastrophic events, an important role should be played by 1) Timely and adequate information to the population. 2) It is necessary to have a sufficient number of beds that can be repurposed in accordance with the needs and provided with appropriate equipment to support the vital functions of the body. 3) Planned training of qualified medical personnel should be carried out. 4) It is necessary to have stocks of emergency drugs close to the institutions where it is possible to receive victims.

Phytolaserophoresis as Integrative Approach to the Treatment of Chronic Internal Organs Diseases

10.12737/5764 ◽  
2014 ◽  
Vol 8 (1) ◽  
pp. 1-7
Author(s):  
Касаева ◽  
E. Kasaeva ◽  
Купеев ◽  
R. Kupeev ◽  
Купеев ◽  
...  
Keyword(s):  
Self Regulation ◽  
The Body ◽  
Integrative Approach ◽  
Therapeutic Effects ◽  
Primary Role ◽  
Endocrine Regulation ◽  
Material Resources ◽  
Non Invasive ◽  
Regulatory Functions

This article describes the development history and basic principles of a modern therapy method - phytolaserophoresis. It provides complex treating impact on chronic diseases, taking into account additional pathogenic disorders of nerve and endocrine regulation. Data analysis of patients with various internal chronic pathologies, revealed the primary role of osteochondrosis and other degenerative disc diseases in maintaining chronic pathological processes. Osteochondrosis doesn’t an isolated problem of the musculoskeletal system, it leads to hypoxia of the brain, the excitement of sub-cortical centers of self-regulation and, as a consequence, in violation of the nervous and endocrine regulation. Treatment by means of phytolaserophoresis method implies soft methods of therapeutic effects on central and peripheral factors to chronic disease. After normalization of the regulatory functions of the body, against the background of recovery of homeostasis and microcirculation in organs, the targeted therapeutic effect at the site of the problem becomes more efficient and can achieve a significant improvement and long-term remission. Advantages of the technique are its non-invasive, absence side effects, available technical and material resources and the possibility of applying the method in medical establishments of any capacity.

scholarly journals Scaling-Up and Semi-Continuous Cultivation of Locally Isolated Marine Microalgae Tetraselmis striata in the Subtropical Island of Gran Canaria (Canary Islands, Spain)

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1326
Author(s):  
Zivan Gojkovic ◽  
Flavio Guidi ◽  
Begoña Bustamante ◽  
Marianna Venuleo ◽  
Patrícia Alexandra Clemente Janeiro de Assunçao ◽  
...  
Keyword(s):  
Large Scale ◽  
Photosynthetic Activity ◽  
Continuous Cultivation ◽  
Scaling Up ◽  
Good Source ◽  
Gran Canaria ◽  
Tetraselmis Striata ◽  
Large Scale Cultivation ◽  
Photosynthetic Efficiencies

The goal of this study was to determine the feasibility of the large-scale cultivation of locally isolated Tetraselmis striata in different open ponds in Gran Canaria. The biomass productivities were 24.66 ± 0.53 kgDW in 32 days (28.9t/ha/year) for 8000 L indoors, 42.32 ± 0.81 kgDW in 43 days (38.8 t/ha/year) for an 8000 L pond outdoors, and 54.9 ± 0.58 kgDW in 28 days (19.6 t/ha/year) for a 45,000 L pond outdoors. The photosynthetic efficiencies were 1.45 ± 0.03% for an 8000 L pond indoors, 1.95 ± 0.04% for 8000 L outdoors. and 1.10 ± 0.01% for a 45,000 L pond outdoors. The selected strain was fast-growing (µ = 0.21 day−1) and could be rapidly scaled up to 45,000 L; it formed healthy cultures that maintained high photosynthetic activity during long-term cultivation and provided stable biomass productivities, able to grow on urea, which acted as a cheap and effective grazer control. The obtained biomass is a good source of proteins and has an FA profile with a high content of some nutritionally important fatty acids: oleic, α-linolenic (ALA) and EPA. The high ash content in the biomass (>35%) can be reduced by the implementation of additional washing steps after the centrifugation of the culture.

scholarly journals Advanced Biomimetic Nanomaterials for Non-invasive Disease Diagnosis

2021 ◽  
Vol 8 ◽  
Author(s):  
Zhiyuan Feng ◽  
Hao Fan ◽  
Lin Cheng ◽  
Huan Zhang ◽  
Hong Fan ◽  
...  
Keyword(s):  
Social Stress ◽  
Large Scale ◽  
Metabolic Diseases ◽  
Disease Diagnosis ◽  
Invasive Disease ◽  
Non Invasive ◽  
Disease Marker ◽  
Medical Materials ◽  
Biomimetic Nanomaterials ◽  
Marker Detection

In modern society, the incidence of cancer, inflammatory diseases, nervous system diseases, metabolic diseases, and cardiovascular diseases is on the rise. These diseases not only cause physical and mental suffering for patients, but also place an enormous burden on society. Early, non-invasive diagnosis of these diseases can reduce the physical and mental pain of patients and social stress. There is an urgent need for advanced materials and methods for non-invasive disease marker detection, large-scale disease screening, and early diagnosis. Biomimetic medical materials are synthetic materials designed to be biocompatible or biodegradable, then developed for use in the medical industry. In recent years, with the development of nanotechnology, a variety of biomimetic medical materials with advanced properties have been introduced. Biomimetic nanomaterials have made great progress in biosensing, bioimaging, and other fields. The latest advance of biomimetic nanomaterials in disease diagnosis has attracted tremendous interest. However, the application of biomimetic nanomaterials in disease diagnosis has not been reviewed. This review particularly focuses on the potential of biomimetic nanomaterials in non-invasive disease marker detection and disease diagnosis. The first part focuses on the properties and characteristics of different kinds of advanced biomimetic nanomaterials. In the second part, the recent cutting-edge methods using biosensors and bioimaging based on biomimetic nanomaterials for non-invasive disease diagnosis are reviewed. In addition, the existing problems and future development of biomimetic nanomaterials is briefly described in the third part. The application of biomimetic nanomaterials would provide a novel and promising diagnostic method for non-invasive disease marker detection, large-scale clinical screening, and diagnosis, promoting the exploitation of devices with better detection performance and the development of global clinical public health.

scholarly journals Long term EIT based compliance monitoring in COVID-19 patients

2021 ◽  
Vol 7 (2) ◽  
pp. 323-326
Author(s):  
Imanol Isasa Reinoso ◽  
Rongqing Chen ◽  
András Lovas ◽  
Knut Moeller
Keyword(s):  
Electrical Impedance ◽  
Disease Diagnosis ◽  
The Body ◽  
Respiratory Complications ◽  
Impedance Tomography ◽  
Non Invasive ◽  
Phenotype Classification ◽  
Continuous Technology

Abstract The COVID-19 is a viral infection that causes respiratory complications. Infected lungs often present ground glass opacities, thus suggesting that medical imaging technologies could provide useful information for the disease diagnosis, treatment, and posterior recovery. The Electrical Impedance Tomography (EIT) is a non-invasive, radiationfree, and continuous technology that generates images by using a sequence of current injections and voltage measurements around the body, making it very appropriate for the study to monitor the regional behaviour of the lung. Moreover, this tool could also be used for a preliminary COVID-19 phenotype classification of the patients. This study is based on the monitoring of lung compliances of two COVID-19-infected patients: the results indicate that one of them could belong to the H-type, while the other is speculated belongs to L-type. It has been concluded that the EIT is a useful tool to obtain information regarding COVID-19 patients and could also be used to classify different phenotypes.

Development of the Mini-BCIBS

2017 ◽  
Vol 33 (6) ◽  
pp. 471-474
Author(s):  
Syd Hiskey ◽  
Fahime Javenbakht ◽  
Nicholas A. Troop
Keyword(s):  
Posttraumatic Growth ◽  
Large Scale ◽  
Female Students ◽  
Internet Survey ◽  
Clinical Samples ◽  
Traumatic Life Events ◽  
Survey Results ◽  
Using Data ◽  
Further Development

Abstract. We describe the development of a brief version of the Bi-Directional Changes in Being Scale (BCIBS; Hiskey, Troop, & Joseph, 2006 ), a measure of phenomenological change following stressful and traumatic life events. The psychometric properties of the mini-BCIBS were explored using data drawn from a sample of female students, survivors of a discotheque fire, and a large-scale Internet survey. Results suggest the new measure retains the breadth of experiences captured by its predecessor and is psychometrically equivalent. The new tool awaits further development among clinical samples and may help researchers explore the long-term trajectory of posttraumatic growth phenomena.

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