scholarly journals A novel animal model for neuroinflammation and white matter degeneration

2017 ◽  
Author(s):  
Baohu Ji ◽  
Kerin Higa ◽  
Virawudh Soontornniyomkij ◽  
Atsushi Miyanohara ◽  
Xianjin Zhou
Keyword(s):  
Animal Model ◽  
Immune System ◽  
White Matter ◽  
Innate Immune System ◽  
D1 Receptor ◽  
Innate Immune ◽  
Small Interference Rna ◽  
Cell Defense ◽  
White Matter Degeneration ◽  
Short Hairpin Rnas

Small interference RNA has been widely used to suppress gene expression. Three different short-hairpin RNAs (shRNAs) against dopamine D1 receptor (Drd1), driven by mouse U6 promoter in self-complementary AAV8 vector (scAAV8), were used to silence mouse striatal Drd1 expression. Transduction of mouse striatum with all 3 scAAV8-D1shRNA virus, but not the control scAAV8 virus, causes extensive neuroinflammation, demyelination, and axon degeneration. RNA interference is known to be coupled to the innate immune system as a host cell defense against virus infection. Activation of the innate immune system may play a causal role in the development of neuroinflammation and white matter degeneration, providing a novel animal model for multiple sclerosis (MS) and other neuroinflammatory diseases.

scholarly journals A novel animal model for neuroinflammation and white matter degeneration

2017 ◽  
Author(s):  
Baohu Ji ◽  
Kerin Higa ◽  
Virawudh Soontornniyomkij ◽  
Atsushi Miyanohara ◽  
Xianjin Zhou
Keyword(s):  
Animal Model ◽  
Immune System ◽  
White Matter ◽  
Innate Immune System ◽  
D1 Receptor ◽  
Innate Immune ◽  
Small Interference Rna ◽  
Cell Defense ◽  
White Matter Degeneration ◽  
Short Hairpin Rnas

Small interference RNA has been widely used to suppress gene expression. Three different short-hairpin RNAs (shRNAs) against dopamine D1 receptor (Drd1), driven by mouse U6 promoter in self-complementary AAV8 vector (scAAV8), were used to silence mouse striatal Drd1 expression. Transduction of mouse striatum with all 3 scAAV8-D1shRNA virus, but not the control scAAV8 virus, causes extensive neuroinflammation, demyelination, and axon degeneration. RNA interference is known to be coupled to the innate immune system as a host cell defense against virus infection. Activation of the innate immune system may play a causal role in the development of neuroinflammation and white matter degeneration, providing a novel animal model for multiple sclerosis (MS) and other neuroinflammatory diseases.

scholarly journals A novel animal model for neuroinflammation and white matter degeneration

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3905 ◽  
Author(s):  
Baohu Ji ◽  
Kerin Higa ◽  
Virawudh Soontornniyomkij ◽  
Atsushi Miyanohara ◽  
Xianjin Zhou
Keyword(s):  
Animal Model ◽  
Immune System ◽  
White Matter ◽  
Innate Immune System ◽  
D1 Receptor ◽  
Innate Immune ◽  
Small Interference Rna ◽  
Cell Defense ◽  
White Matter Degeneration ◽  
Short Hairpin Rnas

Small interference RNA has been widely used to suppress gene expression. Three different short hairpin RNAs (shRNAs) against dopamine D1 receptor (Drd1), driven by mouse U6 promoter in self-complementary AAV8 vector (scAAV8), were used to silence mouse striatal Drd1 expression. Transduction of mouse striatum with all three scAAV8-D1shRNA viruses, but not the control scAAV8 virus, causes extensive neuroinflammation, demyelination, and axon degeneration. RNA interference is known to be coupled to the innate immune system as a host cell defense against virus infection. Activation of the innate immune system may play a causal role in the development of neuroinflammation and white matter degeneration, providing a novel animal model for multiple sclerosis (MS) and other neuroinflammatory diseases.

scholarly journals NLRP3 Inflammasome and MS/EAE

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Makoto Inoue ◽  
Mari L. Shinohara
Keyword(s):  
Multiple Sclerosis ◽  
Animal Model ◽  
Immune System ◽  
Experimental Autoimmune Encephalomyelitis ◽  
Nlrp3 Inflammasome ◽  
Innate Immune System ◽  
Innate Immune ◽  
Autoimmune Encephalomyelitis ◽  
Danger Signals ◽  
Experimental Autoimmune

Inflammasomes are cytosolic sensors that detect pathogens and danger signals in the innate immune system. The NLRP3 inflammasome is currently the most fully characterized inflammasome and is known to detect a wide array of microbes and endogenous damage-associated molecules. Possible involvement of the NLRP3 inflammasome (or inflammasomes) in the development of multiple sclerosis (MS) was suggested in a number of studies. Recent studies showed that the NLRP3 inflammasome exacerbates experimental autoimmune encephalomyelitis (EAE), an animal model of MS, although EAE can also develop without the NLRP3 inflammasome. In this paper, we discuss the NLRP3 inflammasome in MS and EAE development.

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