scholarly journals Effects of exposure to atrazine on retinoid signaling in zebrafish

2017 ◽  
Author(s):  
Yu Fan ◽  
Haixing Liu ◽  
Pengxing Xu ◽  
Weizhi Zhang
Keyword(s):  
Retinoic Acid ◽  
Surface Waters ◽  
Retinoic Acid Receptor ◽  
Binding Protein ◽  
Retinol Binding Protein ◽  
Retinol Dehydrogenase ◽  
Nontarget Organisms ◽  
Retinoid Signaling ◽  
Zebrafish Larvae ◽  
Opsin Genes

Atrazine is a widely used herbicide developed for use in range and pastureland. It is present in many surface waters, contaminating nontarget organisms due to its persistence. In this study, the effects of acute exposure to atrazine on retinoid signaling were investigated in zebrafish. Zebrafish embryos were exposed to atrazine from 6 hours post-fertilization (hpf) to 120 hpf. The contents of retinal and retinoic acid were decreased significantly. The mRNA expression levels of retinal dehydrogenase (raldh2), retinol dehydrogenase (rdh1), retinol binding protein (rbp1a), retinoic acid receptor subunit (raraa), and cellular retinoic acid binding protein (crabp1a and crabp2a) were significantly reduced, which indicated that retinoid signaling was interrupted. However, the transcriptional levels of five opsin genes (zfrho, zfuv, zfred, zfblue, and zfgr1) were increased. These results indicated that exposure to atrazine could inhibit retinoid signaling and impair the eye development of zebrafish larvae.

scholarly journals Effects of exposure to atrazine on retinoid signaling in zebrafish

2017 ◽  
Author(s):  
Yu Fan ◽  
Haixing Liu ◽  
Pengxing Xu ◽  
Weizhi Zhang
Keyword(s):  
Retinoic Acid ◽  
Surface Waters ◽  
Retinoic Acid Receptor ◽  
Binding Protein ◽  
Retinol Binding Protein ◽  
Retinol Dehydrogenase ◽  
Nontarget Organisms ◽  
Retinoid Signaling ◽  
Zebrafish Larvae ◽  
Opsin Genes

Atrazine is a widely used herbicide developed for use in range and pastureland. It is present in many surface waters, contaminating nontarget organisms due to its persistence. In this study, the effects of acute exposure to atrazine on retinoid signaling were investigated in zebrafish. Zebrafish embryos were exposed to atrazine from 6 hours post-fertilization (hpf) to 120 hpf. The contents of retinal and retinoic acid were decreased significantly. The mRNA expression levels of retinal dehydrogenase (raldh2), retinol dehydrogenase (rdh1), retinol binding protein (rbp1a), retinoic acid receptor subunit (raraa), and cellular retinoic acid binding protein (crabp1a and crabp2a) were significantly reduced, which indicated that retinoid signaling was interrupted. However, the transcriptional levels of five opsin genes (zfrho, zfuv, zfred, zfblue, and zfgr1) were increased. These results indicated that exposure to atrazine could inhibit retinoid signaling and impair the eye development of zebrafish larvae.

scholarly journals Effects of exposure to atrazine on retinoid signaling in zebrafish

2017 ◽  
Author(s):  
Yu Fan ◽  
Haixing Liu ◽  
Pengxing Xu ◽  
Weizhi Zhang
Keyword(s):  
Retinoic Acid ◽  
Surface Waters ◽  
Retinoic Acid Receptor ◽  
Binding Protein ◽  
Retinol Binding Protein ◽  
Retinol Dehydrogenase ◽  
Nontarget Organisms ◽  
Retinoid Signaling ◽  
Zebrafish Larvae ◽  
Opsin Genes

Atrazine is a widely used herbicide developed for use in range and pastureland. It is present in many surface waters, contaminating nontarget organisms due to its persistence. In this study, the effects of acute exposure to atrazine on retinoid signaling were investigated in zebrafish. Zebrafish embryos were exposed to atrazine from 6 hours post-fertilization (hpf) to 120 hpf. The contents of retinal and retinoic acid were decreased significantly. The mRNA expression levels of retinal dehydrogenase (raldh2), retinol dehydrogenase (rdh1), retinol binding protein (rbp1a), retinoic acid receptor subunit (raraa), and cellular retinoic acid binding protein (crabp1a and crabp2a) were significantly reduced, which indicated that retinoid signaling was interrupted. However, the transcriptional levels of five opsin genes (zfrho, zfuv, zfred, zfblue, and zfgr1) were increased. These results indicated that exposure to atrazine could inhibit retinoid signaling and impair the eye development of zebrafish larvae.

scholarly journals Evaluation of Topramezone on Zebrafish Retinoid Signaling

2017 ◽  
Author(s):  
Haixing Liu ◽  
Pengxing Xu ◽  
Yu Fan ◽  
Weizhi Zhang
Keyword(s):  
Retinoic Acid ◽  
Retinoic Acid Receptor ◽  
Binding Protein ◽  
Retinol Binding Protein ◽  
Transcriptional Expression ◽  
Retinol Dehydrogenase ◽  
Expression Levels ◽  
Retinoid Signaling ◽  
Weeds Control ◽  
Short Time

ABSTRACTTopramezone is a highly selective herbicide developed for broadleaf and grass weeds control in corn. In this study, the effects of topramezone on zebrafish, especially in retinoid signaling were investigated. Zebrafish embryos were treated with topramezone from 4 hours post-fertilization (hpf) to 144 hpf. Exposed to topramezone significantly reduced the retinal and retinoic levels compared to controls. The transcriptional expression levels of retinol dehydrogenase (rdh1), retinoic acid receptor subunit (raraa), retinal dehydrogenase (raldh2), retinol binding protein (rbp1a), and cellular retinoic acid binding protein (crabp1a and crabp2a) were significantly decreased. Our results suggested that topramezone significantly impaired zebrafish retinoid signaling during a short time exposure. However, treatment with topramezone significantly increased the mRNA expression levels of zfblue, zfrho, zfgr1, zfuv, and zfred. Our data demonstrated that topramezone treatment could interrupt retinoid signaling and further affect zebrafish eye development.

Lung retinol storing cells synthesize and secrete retinoic acid, an inducer of alveolus formation

2004 ◽  
Vol 286 (2) ◽  
pp. L249-L256 ◽  
Author(s):  
Ghenima Dirami ◽  
Gloria DeCarlo Massaro ◽  
Linda Biadasz Clerch ◽  
Una S. Ryan ◽  
Peter R. Reczek ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Retinoic Acid Receptor ◽  
Retinol Binding Protein ◽  
Microvascular Endothelial Cell ◽  
Alveolar Wall ◽  
Retinoid Signaling ◽  
All Trans Retinoic Acid ◽  
Wall Cell ◽  
Pulmonary Microvascular Endothelial Cell ◽  
Storage Granules

Retinoids play a key role in the formation of pulmonary alveoli. Lipid interstitial cells (LICs) of the alveolar wall store retinol and are concentrated at sites of alveolus formation, suggesting they are an endogenous source of retinoids for alveolus formation. We show in cultured rat lung cells that LICs synthesize and secrete all- trans retinoic acid (ATRA); its secretion is halved by dexamethasone, an inhibitor of alveolus formation. In a second alveolar wall cell, the pulmonary microvascular endothelial cell (PMVC), ATRA increases expression of the mRNA of cellular retinol binding protein-I (CRBP-I), a protein involved in ATRA synthesis. Serum-free, exogenous ATRA-free medium conditioned by LICs rich in retinol storage granules caused a 10-fold greater increase of CRBP-I mRNA in PMVCs than media conditioned by LICs with few retinol storage granules. This action of medium conditioned by retinol storage granule-rich LICs is decreased by a retinoic acid receptor pan-antagonist and by a retinoid X receptor pan-antagonist, suggesting the responsible molecule(s) is a retinoid and that retinoid signaling occurs in a paracrine fashion.

scholarly journals Estrogen Directly Induces Expression of Retinoic Acid Biosynthetic Enzymes, Compartmentalized between the Epithelium and Underlying Stromal Cells in Rat Uterus

Endocrinology ◽  
2004 ◽  
Vol 145 (10) ◽  
pp. 4756-4762 ◽  
Author(s):  
Xiao-Hong Li ◽  
Bharati Kakkad ◽  
David E. Ong
Keyword(s):  
Retinoic Acid ◽  
Stromal Cells ◽  
Binding Protein ◽  
Retinol Binding Protein ◽  
Mrna Levels ◽  
Retinol Dehydrogenase ◽  
Rat Uterus ◽  
Stromal Compartment ◽  
Specific Localization ◽  
Prior Administration

Abstract Estrogen (E2) has been shown to induce the biosynthesis of retinoic acid (RA) in rat uterus. Here we examined whether E2 could directly induce the enzymes involved in this process by using the ovariectomized rat. A retinol dehydrogenase that we have previously described, eRolDH, and the retinal dehydrogenase, RalDH II, were found to have markedly increased uterine mRNA levels within 4 h of E2 administration, independent of the prior administration of puromycin. eRolDH and RalDH II and their mRNAs were also increased in uteri of rats during estrus. This indicated that RA biosynthesis in rat uterus is directly controlled by E2 and provides a direct link between the action of a steroid hormone and retinoid action. We also examined the cell-specific localization of RalDH II by immunohistochemistry. The enzyme was observed in the stromal compartment, particularly in cells close to the uterine lumenal epithelium. eRolDH was observed only in the lining epithelial cells. Taken together with the previous observations of cellular retinol-binding protein and cellular retinoic acid-binding protein, type two also being expressed in the lumenal epithelium, we propose that RA production is compartmentalized, with retinol oxidation occurring in the lumenal epithelium and subsequent oxidation of retinal to RA occurring in the underlying stromal cells.

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