scholarly journals Impact of polybasic alcohols on biocompatibility and selectivity of Penicillin G Acylase for kinetically controlled synthesis

2015 ◽  
Author(s):  
Yi-Feng Shi ◽  
Zhu-An Cao ◽  
Zhong-Yao Shen
Keyword(s):  
Methyl Ester ◽  
Ethylene Glycol ◽  
Butyl Alcohol ◽  
Penicillin G Acylase ◽  
Penicillin G ◽  
Acyl Donor ◽  
Controlled Synthesis ◽  
Kinetically Controlled ◽  
Hydrolysis Of ◽  
Kinetically Controlled Synthesis

The enzyme catalyzed synthesis of cephalexin (CEX) from 7-amino-3-deacetoxycephalosporanic acid (7-ADCA) and D-a-phenylglycine methyl ester (PGM) by Penicillin G acylase (PGA) is a model for kinetically controlled synthesis. The parallel hydrolysis of PGM, the activated acyl donor, is the principle competing pathway in this reaction, limiting the synthetic yield and reaction efficiency. To improve the performance of PGA catalyzed CEX synthesis, the biocompatibility and selectivity of various co-solvents were investigated. Polybasic alcohols such as ethylene glycol, glycerol and PEG400 did not cause deleterious changes to the enzyme, whereas monobasic alcohols, such as butyl alcohol, disrupted the PGA activity. Compared with the reaction in aqueous medium, the use of ethylene glycol as a co-solvent was found to have good selectivity in order to facilitate CEX synthesis and significantly minimize PGM hydrolysis. The pH of ethylene glycol medium was also optimized. The mechanism of the enhanced effect of polybasic alcohols as co-solvents on both biocompatibility and selectivity of enzymatic kinetically controlled synthesis is suggested.

scholarly journals Impact of polybasic alcohols on biocompatibility and selectivity of Penicillin G Acylase for kinetically controlled synthesis

2015 ◽  
Author(s):  
Yi-Feng Shi ◽  
Zhu-An Cao ◽  
Zhong-Yao Shen
Keyword(s):  
Methyl Ester ◽  
Ethylene Glycol ◽  
Butyl Alcohol ◽  
Penicillin G Acylase ◽  
Penicillin G ◽  
Acyl Donor ◽  
Controlled Synthesis ◽  
Kinetically Controlled ◽  
Hydrolysis Of ◽  
Kinetically Controlled Synthesis

The enzyme catalyzed synthesis of cephalexin (CEX) from 7-amino-3-deacetoxycephalosporanic acid (7-ADCA) and D-a-phenylglycine methyl ester (PGM) by Penicillin G acylase (PGA) is a model for kinetically controlled synthesis. The parallel hydrolysis of PGM, the activated acyl donor, is the principle competing pathway in this reaction, limiting the synthetic yield and reaction efficiency. To improve the performance of PGA catalyzed CEX synthesis, the biocompatibility and selectivity of various co-solvents were investigated. Polybasic alcohols such as ethylene glycol, glycerol and PEG400 did not cause deleterious changes to the enzyme, whereas monobasic alcohols, such as butyl alcohol, disrupted the PGA activity. Compared with the reaction in aqueous medium, the use of ethylene glycol as a co-solvent was found to have good selectivity in order to facilitate CEX synthesis and significantly minimize PGM hydrolysis. The pH of ethylene glycol medium was also optimized. The mechanism of the enhanced effect of polybasic alcohols as co-solvents on both biocompatibility and selectivity of enzymatic kinetically controlled synthesis is suggested.

scholarly journals Gold Nanoparticles for Colorimetric detection of hydrolysis of antibiotics by penicillin G acylase

2010 ◽  
Vol 01 (04) ◽  
pp. 322-329 ◽  
Author(s):  
Neha R. Tiwari ◽  
Ambrish Rathore ◽  
Asmita Prabhune ◽  
Sulabha K. Kulkarni
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Detection ◽  
Penicillin G Acylase ◽  
Penicillin G ◽  
Hydrolysis Of

Kinetically controlled synthesis of ampicillin and cephalexin in highly condensed systems in the absence of a liquid aqueous phase

2006 ◽  
Vol 39 (1-4) ◽  
pp. 105-111 ◽  
Author(s):  
Alessandra Basso ◽  
Patrizia Spizzo ◽  
Micaela Toniutti ◽  
Cynthia Ebert ◽  
Paolo Linda ◽  
...  
Keyword(s):  
Aqueous Phase ◽  
Controlled Synthesis ◽  
Kinetically Controlled ◽  
Condensed Systems ◽  
Kinetically Controlled Synthesis

Kinetically controlled synthesis of nickel tetrahedron nanocrystals for high performance catalytic hydrogenation

RSC Advances ◽  
2013 ◽  
Vol 3 (16) ◽  
pp. 5314 ◽  
Author(s):  
Guoxiang Chen ◽  
Yonghui Zhao ◽  
Dong Lv ◽  
Tiejun Zhao ◽  
Yan Zhu ◽  
...  
Keyword(s):  
Catalytic Hydrogenation ◽  
High Performance ◽  
Controlled Synthesis ◽  
Kinetically Controlled ◽  
Kinetically Controlled Synthesis

Growth pathways of silver nanoplates in kinetically controlled synthesis: bimodal versus unimodal growth

RSC Advances ◽  
2015 ◽  
Vol 5 (19) ◽  
pp. 14266-14272 ◽  
Author(s):  
Mun Ho Kim ◽  
Dong Ki Yoon ◽  
Sang Hyuk Im
Keyword(s):  
Reducing Agent ◽  
Controlled Synthesis ◽  
Silver Nanoplates ◽  
Kinetically Controlled ◽  
Kinetically Controlled Synthesis

In the kinetically controlled synthesis, the growth of Ag nanoplates proceeded along different pathways depending on the concentration of PVP serving as both a reducing agent and a stabilizer.

Kinetically controlled synthesis of bimetallic nanostructures by flowrate manipulation in a continuous flow droplet reactor

2018 ◽  
Vol 3 (4) ◽  
pp. 437-441 ◽  
Author(s):  
Joshua S. Santana ◽  
Kallum M. Koczkur ◽  
Sara E. Skrabalak
Keyword(s):  
Continuous Flow ◽  
Pd Nanoparticles ◽  
Core Shell ◽  
Controlled Synthesis ◽  
Seeded Growth ◽  
Kinetically Controlled ◽  
Bimetallic Nanostructures ◽  
Kinetically Controlled Synthesis

We show that different Au–Pd nanoparticles, ranging from sharp-branched octopods to core@shell octahedra, can be achieved by inline manipulation of reagent flowrates in a microreactor for seeded growth.

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