Histological examination of bone tissue for comparison of three different agents decalcification

2014 ◽  
Keyword(s):  
Bone Tissue ◽  
Histological Examination

Bone Tissue Ablation with sub-µs Pulses of a Q-switch CO 2 Laser: Histological Examination of Thermal Side Effects

2002 ◽  
Vol 17 (4) ◽  
pp. 258-264 ◽  
Author(s):  
M.M. Ivanenko ◽  
S. Fahimi-Weber ◽  
T. Mitra ◽  
W. Wierich ◽  
P. Hering
Keyword(s):  
Side Effects ◽  
Bone Tissue ◽  
Histological Examination ◽  
Tissue Ablation

scholarly journals A histological evaluation of bone calluses in the treatment of tibia fractures in sheep with the use of a semicircular fixator 

2010 ◽  
Vol 55 (No. 11) ◽  
pp. 547-550
Author(s):  
Z. Adamiak ◽  
T. Rotkiewicz
Keyword(s):  
Fracture Healing ◽  
Bone Tissue ◽  
Histological Examination ◽  
Bone Growth ◽  
Compact Bone ◽  
Tibia Fracture ◽  
Histological Evaluation ◽  
Fracture Treatment ◽  
Compact Bone Tissue ◽  
Tibia Fractures

This study discusses the results of a histological examination of bone calluses in the treatment of tibia fractures in sheep with the involvement of a semi-circular fixator. In all sheep, callus samples revealed the presence of well-developed, compact bone tissue in the area of fracture healing. It was found that the use of a semi-circular fixator promoted bone growth, and that it is an effective method for tibia fracture treatment in sheep.

scholarly journals Post COVID-19 invasive mucormycosis and actinomycosis co-infection: a case report

2021 ◽  
Author(s):  
Neha Mishra ◽  
Venkata Sai Shashank Mutya ◽  
Irfan Ibrahim K. ◽  
Girish Rai
Keyword(s):  
Case Report ◽  
Bone Tissue ◽  
Histological Examination ◽  
Surgical Management ◽  
Gold Standard ◽  
Bacterial Culture ◽  
Beta Lactams ◽  
Necrotic Bone ◽  
Sulfur Granules ◽  
Aggressive Debridement

<p>Actinomyces are commensals of human oropharynx and actinomycosis is considered mainly as an endogenous infection that is triggered by a mucosal lesion. Typically, the disease presents as a slowly progressive painless indurated mass evolving into multiple abscesses with draining sinus tracts sometimes expressing a typical yellow exudate with characteristic sulfur granules. The gold standard of diagnosis is histological examination and bacterial culture of the tissue. Most isolates are susceptible to beta lactams and they are the treatment of choice along with surgical management with drainage of abscesses and excision of recalcitrant fibrotic lesions and debridement of necrotic bone tissue. Here we present a case of 37-year-old male patient who has developed severe COVID-19 infection following which he developed invasive mucormycosis followed by actinomycosis. We postulate that the lymphopenia and the use of immunosuppressants used in treatment of COVID-19 lead to mucormycosis and aggressive debridement used as a strategy in treatment of mucormycosis led to colonization of actinomyces leading to cervicofacial actinomycosis.</p>

scholarly journals A HISTOLOGICAL PICTURE OF BONE TISSUE IN CHILDREN WITH HEMATOGENOUS OSTEOMYELITIS

2019 ◽  
Vol 23 (5) ◽  
pp. 254-257
Author(s):  
S. V. Minaev ◽  
Alina N. Grigorova ◽  
N. V. Filipeva ◽  
I. N. Gerasimenko ◽  
O. I. Sevryukova ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Tissue ◽  
Histological Examination ◽  
T Lymphocyte ◽  
Chronic Osteomyelitis ◽  
City Hospital ◽  
Double Positive ◽  
Red Bone Marrow ◽  
Hematogenous Osteomyelitis ◽  
Acute Hematogenous Osteomyelitis

Introduction. Currently, there is a limited number of works devoted to the comprehensive study of specific features of surgical treatment of patients with acute hematogenous osteomyelitis (AHO) which includes histological and immune histochemical (IHC) tests of the bone marrow at different periods of the disease. In this regard, the purpose of our work was to make the morphological analyses of bone tissue and bone marrow fragments in children with hematogenous osteomyelitis. Material and methods. In 2013- 2016, in the department of purulent surgery of the city hospital in Stavropol, there were 64 children with AHO of long tubular bones aged from 3 to 17. 47 boys (73.4%), 17 girls (26.6%). The lesion was located in : femur - 25 (39.1%) children; tibia - 24 (37.5%); fibula - 6 (9.4%); humerus - 5 (7.8%); ulna - 3 (4.7%); radius - 1 (1.5%). All children had urgent osteoperforation of the affected bone. Bone slices were taken for histological examination and bone marrow fragments for IHC examination. Tissue sections were stained with hematoxylin and eosin, pikrofuksin by Van Gieson, aniline blue by Mallory, trichrome by Masson. IHC examination was done using a standard protocol with anti-CD3, CD4, CD8 monoclonal antibodies. Results. In AHO, the histological examination has revealed fragments of spongy bone tissue, elements of yellow and red bone marrow with diffuse abundant leukocyte infiltration, hemorrhages in bone marrow spaces, microcore fractures and necrotic foci in the trabeculae, vast areas of pronounced autolytic resorption of the bone tissue. Accumulation of CD3+cells (2-4) is seen in the inflammation infiltrate areas located along the fistula passage. The expression of T-lymphocyte-helper markers, or CD4 +, is moderate in chronic osteomyelitis (2 points). The expression of T-lymphocyte suppressor marker, or CD8 +, is moderate in chronic osteomyelitis (2 points). Immunoreactive material - coarse granular, cytoplasmic, disseminated (3 points). CD8 + lymphocytes are seen in clusters of cells in the amount from 4-6 to 20-30. In addition, to our mind, the picture of structural location of lymphocytes, is not completed, since cells are moving to the zone of interaction of CD8 + lymphocytes. Conclusions. Thus, minor subpopulations of lymphocytes, in particular double-positive T cells, or CD3 + / CD4 + / CD8 + lymphocytes, or highly differentiated memory cells are registered in the red bone marrow of patients with AHO. It characterizes the immunoregulatory index of the organism and may indicate the presence of autoimmune component in the immune response. If even separate CD8 + lymphocytes are revealed in the bone marrow of children with hematogenous osteomyelitis at IHC, it may indicate a risk of complications.

scholarly journals Clinical Case of Tracheobronchopathia Osteochondroplastica

2016 ◽  
Vol 23 (4) ◽  
pp. 201641
Author(s):  
Nataliia V. Chaplynska ◽  
Mykola M. Bagrii ◽  
Victoriia T. Rudnyk ◽  
Mariana Y. Koniuska ◽  
Svitlana L. Filipova ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Histological Examination ◽  
Late Onset ◽  
Main Bronchus ◽  
Clinical Manifestations ◽  
Differential Diagnostics ◽  
Bronchial Obstruction ◽  
Virtual Bronchoscopy ◽  
Subepithelial Layer ◽  
Tracheobronchopathia Osteochondroplastica

Tracheobronchopathia osteochondroplastica (TO) is a rare chronic disease of the airways characterized by the growth of cartilage and/or bone tissue in the submucosal layer of the trachea and large bronchi with varying degrees of their lumen narrowing. In a significant percentage of cases diagnosis is made only posthumously due to the late onset of clinical manifestations and the considerable complexity of life-time diagnostics.The objective of the research was to conduct the clinical observation of OT.Materials and methods. A patient Sh., 49 years of age, was admitted to the department of differential diagnostics of regional clinical pulmonology center to determine the cause of prolonged cough and bronchial obstruction syndrome. Only fiber-optic bronchoscopy (FBS) was the only informative method of examination. On the mucous membrane of the trachea and right main bronchus the dense white protuberance was found. The biopsy material was taken. Histological examination revealed areas of calcified cartilage located in subepithelial layer. Results. Taking into account the reasearch data, the TO was suspected. The disease has a benign course for many years and is asymptomatic at initial stages. However, deformation and narrowing of the lumen of the trachea and the large bronchi with epithelial atrophy causes a disturbance of phlegm discharge. This promotes the development of infectious and inflammatory processes in the lung tissue. Patients complain of cough, hemoptysis, shortness of breath, chest pain. X-ray examination of the lungs may detect the infiltrative changes. The indicators of lung function are normal or correspond to the type of obstructive disorders. CT radiograph and virtual bronchoscopy determine the deformation and narrowing of the trachea and the large bronchi due to the elements of cartilage and bone tissue. Definitive diagnosis is possible only according to the results of FBS and histological examination.Conclusions. Awareness on such rare pathology as TO is essential, especially in patients with chronic cough. In case of absence of specific clinical and radiographic changes, the computed tomography of the chest and FBS with for histological examination sampling should be performed to confirm the diagnosis.

Sternal augmentation plasty utilizing homologous bone tissue after Fontan operation

2010 ◽  
Vol 58 (S 01) ◽  
Author(s):  
W Kuroczynski ◽  
C Kampmann ◽  
R Huth ◽  
M Hartert ◽  
M Heinemann ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Fontan Operation

Mineral composition and gemological characteristics of the fossil marine reptiles of the Ulyanovsk region

2018 ◽  
pp. 12-16 ◽  
Author(s):  
D. A. Petrochenkov
Keyword(s):  
Chemical Composition ◽  
Bone Tissue ◽  
Mineral Composition ◽  
Bone Structure ◽  
Upper Jurassic ◽  
Marine Reptiles ◽  
Ulyanovsk Region ◽  
Impurity Elements ◽  
Wide Assortment

Fossils of marine reptiles are a new jewelry and ornamental material and collected in the Ulyanovsk region from the Upper Jurassic deposits. They consist of (wt. %): calcite — 52, apatite — 24 and pyrite — 23, and also gypsum presents. The contents of radioactive and carcinogenic elements are close to background. The original bone structure of reptiles is preserved. Apatite replaces the bone tissue of marine reptiles, forming a cellular framework. According to the chemical composition, apatite refers to fluorohydroxyapatite with an increased Sr content. The size of the crystals is finely-dispersed. Calcite and pyrite fill the central parts of the cells. Calcite crystals of isometric and elongated shape, 0,01—0,05 mm in size, form blocks up to 0,3 mm during intergrowth. Calcite fills thin, discontinuous veins along the contour of cells with a width of up to 0,03 mm. In calcite, among the impurity elements, there are (wt. %, on the average): Mg — 0,30, Mn — 0,39 and Fe — 0,96. Pyrite forms a dispersed impregnation in calcite and apatite, content of impurities is, wt. %: Ni — up to 0,96 and Cu — up to 0,24. On technological and decorative characteristics of fossils of sea reptiles of Ulyanovsk region are qualitative jewelry and ornamental materials of biomineral group, allowing to make a wide assortment of jewelry and souvenir products.

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

Sign in / Sign up

Export Citation Format

Share Document