scholarly journals Isolation and purification of blasticidin s deaminase from Aspergillus terreus.

1975 ◽  
Vol 28 (1) ◽  
pp. 7-14 ◽  
Author(s):  
ISAMU YAMAGUCHI ◽  
HIROKO SHIBATA ◽  
HARUO SETO ◽  
TOMOMASA MISATO
Keyword(s):  
Aspergillus Terreus ◽  
Isolation And Purification ◽  
Blasticidin S

scholarly journals 2P056 Spectroscopic Studies on the Cobalt-Substituted Blasticidin S deaminase from Aspergillus terreus

2005 ◽  
Vol 45 (supplement) ◽  
pp. S133
Author(s):  
M. Nosaka ◽  
T. Higuchi ◽  
M. Furuichi ◽  
T. Kohzuma
Keyword(s):  
Aspergillus Terreus ◽  
Spectroscopic Studies ◽  
Blasticidin S

An unexpected gift from fungicide metabolism studies: blasticidin S deaminase (BSD) from Aspergillus terreus

2002 ◽  
pp. 55-60
Author(s):  
Makoto Kimura ◽  
Masaki Yamamoto ◽  
Makio Furuichi ◽  
Takashi Kumasaka ◽  
Isamu Yamaguchi
Keyword(s):  
Aspergillus Terreus ◽  
Blasticidin S

Crystallization and preliminary X-ray diffraction studies of blasticidin S deaminase from Aspergillus terreus

1999 ◽  
Vol 55 (2) ◽  
pp. 547-548 ◽  
Author(s):  
Masayoshi Nakasako ◽  
Makoto Kimura ◽  
Isamu Yamaguchi
Keyword(s):  
Diffraction Data ◽  
Aspergillus Terreus ◽  
Crystal Structure Analysis ◽  
Thin Plates ◽  
X Ray Diffraction ◽  
Orthorhombic Space Group ◽  
Cell Dimensions ◽  
Crystallization Conditions ◽  
Blasticidin S ◽  
Unit Cell Dimensions

Blasticidin S deaminase from Aspergillus terreus was crystallized with polyethylene glycol 8000. Two types of crystals were grown under the same crystallization conditions. One type grew as thin plates, while the other had a rhombic shape. The rhombic shaped crystal was suitable for high-resolution crystal structure analysis. Precession photographs and diffraction data showed that the crystal belonged to orthorhombic space group P212121, with unit-cell dimensions a = 70.33, b = 146.56 and c = 56.48 Å. The calculated Vm value was acceptable when a tetramer of the enzyme was contained in an asymmetric unit. Preliminary diffraction data were collected to a resolution of 2.0 Å with good statistics.

The Flexible C-Terminal Region of Aspergillus terreus Blasticidin S Deaminase: Identification of Its Functional Roles with Deletion Enzymes

2002 ◽  
Vol 290 (1) ◽  
pp. 421-426 ◽  
Author(s):  
Makoto Kimura ◽  
Makio Furuichi ◽  
Masaki Yamamoto ◽  
Takashi Kumasaka ◽  
Hiroshi Mizuno ◽  
...  
Keyword(s):  
Aspergillus Terreus ◽  
Terminal Region ◽  
Functional Roles ◽  
Blasticidin S

Immunogold labeling of CMP-KDO synthetase produced by recombinant E. Coli cells

1987 ◽  
Vol 45 ◽  
pp. 924-925
Author(s):  
F. A. Durum ◽  
R. G. Goldman ◽  
T. J. Bolling ◽  
M. F. Miller
Keyword(s):  
Inclusion Bodies ◽  
Large Scale ◽  
Recombinant Dna ◽  
Test System ◽  
Cell Protein ◽  
Gram Negative Bacteria ◽  
Cytoplasmic Inclusions ◽  
Isolation And Purification ◽  
E Coli ◽  
Low Concentrations

CMP-KDO synthetase (CKS) is an enzyme which plays a key role in the synthesis of LPS, an outer membrane component unique to gram negative bacteria. CKS activates KDO to CMP-KDO for incorporation into LPS. The enzyme is normally present in low concentrations (0.02% of total cell protein) which makes it difficult to perform large scale isolation and purification. Recently, the gene for CKS from E. coli was cloned and various recombinant DNA constructs overproducing CKS several thousandfold (unpublished data) were derived. Interestingly, no cytoplasmic inclusions of overproduced CKS were observed by EM (Fig. 1) which is in contrast to other reports of large proteinaceous inclusion bodies in various overproducing recombinant strains. The present immunocytochemical study was undertaken to localize CKS in these cells.Immune labeling conditions were first optimized using a previously described cell-free test system. Briefly, this involves soaking small blocks of polymerized bovine serum albumin in purified CKS antigen and subjecting them to various fixation, embedding and immunochemical conditions.

Asperjinone, a norneolignan, and terrein, a suppressor of ABCG2-expressing breast cancer cells, from Aspergillus terreus

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
WY Liao ◽  
CN Shen ◽  
LH Lin ◽  
YL Yang ◽  
HY Han ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Aspergillus Terreus
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