scholarly journals Non-invasive longitudinal imaging of VEGF-induced microvascular alterations in skin wounds

Theranostics ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 558-573
Author(s):  
Yu-Hang Liu ◽  
Lorenz M. Brunner ◽  
Johannes Rebling ◽  
Maya Ben-Yehuda Greenwald ◽  
Sabine Werner ◽  
...  
Keyword(s):  
Skin Wounds ◽  
Non Invasive ◽  
Microvascular Alterations ◽  
Longitudinal Imaging

scholarly journals In Vivo Imaging of Biodegradable Implants and Related Tissue Biomarkers

Polymers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 2348
Author(s):  
Leon Riehakainen ◽  
Chiara Cavallini ◽  
Paolo Armanetti ◽  
Daniele Panetta ◽  
Davide Caramella ◽  
...  
Keyword(s):  
Imaging Modality ◽  
Imaging Techniques ◽  
Special Focus ◽  
Tissue Remodelling ◽  
Biodegradable Implant ◽  
Advantages And Disadvantages ◽  
Non Invasive ◽  
Positron Emission ◽  
Longitudinal Imaging

Non-invasive longitudinal imaging of osseointegration of bone implants is essential to ensure a comprehensive, physical and biochemical understanding of the processes related to a successful implant integration and its long-term clinical outcome. This study critically reviews the present imaging techniques that may play a role to assess the initial stability, bone quality and quantity, associated tissue remodelling dependent on implanted material, implantation site (surrounding tissues and placement depth), and biomarkers that may be targeted. An updated list of biodegradable implant materials that have been reported in the literature, from metal, polymer and ceramic categories, is provided with reference to the use of specific imaging modalities (computed tomography, positron emission tomography, ultrasound, photoacoustic and magnetic resonance imaging) suitable for longitudinal and non-invasive imaging in humans. The advantages and disadvantages of the single imaging modality are discussed with a special focus on preclinical imaging for biodegradable implant research. Indeed, the investigation of a new implant commonly requires histological examination, which is invasive and does not allow longitudinal studies, thus requiring a large number of animals for preclinical testing. For this reason, an update of the multimodal and multi-parametric imaging capabilities will be here presented with a specific focus on modern biomaterial research.

scholarly journals Longitudinal Imaging Using PET/CT with Collagen-I PET-Tracer and MRI for Assessment of Fibrotic and Inflammatory Lesions in a Rat Lung Injury Model

2020 ◽  
Vol 9 (11) ◽  
pp. 3706 ◽  
Author(s):  
Irma Mahmutovic Persson ◽  
Nina Fransén Pettersson ◽  
Jian Liu ◽  
Hanna Falk Håkansson ◽  
Anders Örbom ◽  
...  
Keyword(s):  
Lung Injury ◽  
Imaging Techniques ◽  
Collagen I ◽  
Imaging Biomarkers ◽  
Drug Induced ◽  
Non Invasive ◽  
Pet Tracer ◽  
Pet Ct ◽  
Activity Measurements ◽  
Longitudinal Imaging

Non-invasive imaging biomarkers (IBs) are warranted to enable improved diagnostics and follow-up monitoring of interstitial lung disease (ILD) including drug-induced ILD (DIILD). Of special interest are IB, which can characterize and differentiate acute inflammation from fibrosis. The aim of the present study was to evaluate a PET-tracer specific for Collagen-I, combined with multi-echo MRI, in a rat model of DIILD. Rats were challenged intratracheally with bleomycin, and subsequently followed by MRI and PET/CT for four weeks. PET imaging demonstrated a significantly increased uptake of the collagen tracer in the lungs of challenged rats compared to controls. This was confirmed by MRI characterization of the lesions as edema or fibrotic tissue. The uptake of tracer did not show complete spatial overlap with the lesions identified by MRI. Instead, the tracer signal appeared at the borderline between lesion and healthy tissue. Histological tissue staining, fibrosis scoring, lysyl oxidase activity measurements, and gene expression markers all confirmed establishing fibrosis over time. In conclusion, the novel PET tracer for Collagen-I combined with multi-echo MRI, were successfully able to monitor fibrotic changes in bleomycin-induced lung injury. The translational approach of using non-invasive imaging techniques show potential also from a clinical perspective.

scholarly journals Non-invasive imaging reveals conditions that impact distribution and persistence of cells after in vivo administration

2017 ◽  
Author(s):  
Lauren Scarfe ◽  
Arthur Taylor ◽  
Jack Sharkey ◽  
Rachel Harwood ◽  
Michael Barrow ◽  
...  
Keyword(s):  
Cell Types ◽  
Arterial System ◽  
Imaging Modalities ◽  
Organ Distribution ◽  
Human Umbilical Cord ◽  
Cell Therapies ◽  
Non Invasive ◽  
Longitudinal Imaging ◽  
In Vivo Administration

AbstractBackgroundCell-based regenerative medicine therapies are now frequently tested in clinical trials. In many conditions, cell therapies are administered systemically, but there is little understanding of their fate, and adverse events are often under-reported. Currently, it is only possible to assess safety and fate of cell therapies in preclinical studies, specifically by monitoring animals longitudinally using multimodal imaging approaches. Here, using a suite of in vivo imaging modalities to explore the fate of a range of human and murine cells, we investigate how route of administration, cell type and host immune status affect the fate of administered cells.MethodsWe applied a unique imaging toolkit combining bioluminescence, optoacoustic and magnetic resonance imaging modalities to assess the safety of different human and murine cell types by following their biodistribution and persistence in mice following administration into the venous or arterial system. Results: Longitudinal imaging analyses (i) suggested that the intra-arterial route may be more hazardous than intravenous administration for certain cell types; (ii) revealed that the potential of a mouse mesenchymal stem/stromal cell (MSC) line to form tumours, depended on administration route and mouse strain; and (iii) indicated that clinically tested human umbilical cord (hUC)-derived MSCs can transiently and unexpectedly proliferate when administered intravenously to mice.ConclusionsIn order to perform an adequate safety assessment of potential cell-based therapies, a thorough understanding of cell biodistribution and fate post administration is required. The non-invasive imaging toolbox used here can expose not only the general organ distribution of these therapies, but also a detailed view of their presence within different organs and, importantly, tumourigenic potential. Our observation that the hUC-MSCs but not the human bone marrow (hBM)-derived MSCs persisted for a period in some animals, suggests that therapies with these cells should proceed with caution.

scholarly journals Bellymount enables longitudinal, intravital imaging of abdominal organs and the gut microbiota in adult Drosophila

2019 ◽  
Author(s):  
Leslie Ann Jaramillo Koyama ◽  
Andrés Aranda-Díaz ◽  
Yu-Han Su ◽  
Shruthi Balachandra ◽  
Judy Lisette Martin ◽  
...  
Keyword(s):  
Tissue Level ◽  
Microbial Colonization ◽  
Internal Organs ◽  
Cell Lineages ◽  
Non Invasive ◽  
Abdominal Organs ◽  
Invasive Method ◽  
Longitudinal Imaging ◽  
Long Timescales ◽  
Adult Drosophila

Cell- and tissue-level processes often occur across days or weeks, but few imaging methods can capture such long timescales. Here we describe Bellymount, a simple, non-invasive method for longitudinal imaging of the Drosophila abdomen at sub-cellular resolution. Bellymounted flies remain live and intact, so the same individual can be imaged serially to yield vivid time series of multi-day processes. This feature opens the door to longitudinal studies of Drosophila internal organs in their native context. Exploiting Bellymount’s capabilities, we track intestinal stem cell lineages and gut microbial colonization in single flies, revealing spatiotemporal dynamics undetectable by previously available methods.

X-ray microtomography

1988 ◽  
Vol 46 ◽  
pp. 998-999
Author(s):  
H.W. Deckman ◽  
B.F. Flannery ◽  
J.H. Dunsmuir ◽  
K.D' Amico
Keyword(s):  
Computed Tomography ◽  
Internal Structure ◽  
Imaging Technique ◽  
Three Dimensional ◽  
Tissue Structure ◽  
Individual Element ◽  
X Ray ◽  
Non Invasive ◽  
Panoramic Imaging ◽  
Three Dimensional Images

We have developed a new X-ray microscope which produces complete three dimensional images of samples. The microscope operates by performing X-ray tomography with unprecedented resolution. Tomography is a non-invasive imaging technique that creates maps of the internal structure of samples from measurement of the attenuation of penetrating radiation. As conventionally practiced in medical Computed Tomography (CT), radiologists produce maps of bone and tissue structure in several planar sections that reveal features with 1mm resolution and 1% contrast. Microtomography extends the capability of CT in several ways. First, the resolution which approaches one micron, is one thousand times higher than that of the medical CT. Second, our approach acquires and analyses the data in a panoramic imaging format that directly produces three-dimensional maps in a series of contiguous stacked planes. Typical maps available today consist of three hundred planar sections each containing 512x512 pixels. Finally, and perhaps of most import scientifically, microtomography using a synchrotron X-ray source, allows us to generate maps of individual element.

Non-invasive measurement of a fermentation pattern shift in infants during the first three months of life

2001 ◽  
Vol 120 (5) ◽  
pp. A266-A266
Author(s):  
R BUTLER ◽  
B ZACHARAKIS ◽  
D MOORE ◽  
K CRAWFORD ◽  
G DAVIDSON ◽  
...  
Keyword(s):  
Non Invasive ◽  
Fermentation Pattern ◽  
Invasive Measurement

European multicentre validation trial of two new non-invasive tests for detection of antibody to H. pylori: Urine-based ELISA and rapid urine test

2001 ◽  
Vol 120 (5) ◽  
pp. A491-A491 ◽  
Author(s):  
A LEODOLTER ◽  
D VAIRA ◽  
F BAZZOLL ◽  
A HIRSCHL ◽  
F MEGRAUD ◽  
...  
Keyword(s):  
Urine Test ◽  
Non Invasive ◽  
H Pylori

Tu1996 NON-INVASIVE BREATH TEST SCREENING TO ASSESS FOR PREPROCEDURAL FASTING USING AN ELECTRONIC NOSE: AN UPDATE

2020 ◽  
Vol 158 (6) ◽  
pp. S-1249
Author(s):  
Yuri Hanada ◽  
Juan Reyes Genere ◽  
Bryan Linn ◽  
Tiffany Mangels-Dick ◽  
Kenneth K. Wang
Keyword(s):  
Electronic Nose ◽  
Breath Test ◽  
Non Invasive

1307: Renal Radiosurgery and Sorafenib as Non-Invasive Nephron-Sparing Treatment of Small Localized Renal Masses

2007 ◽  
Vol 177 (4S) ◽  
pp. 430-430
Author(s):  
Ram Ganapathi ◽  
Troy R. Gianduzzo ◽  
Arul Mahadevan ◽  
Monish Aron ◽  
Lee E. Ponsky ◽  
...  
Keyword(s):  
Renal Masses ◽  
Non Invasive ◽  
Nephron Sparing
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