scholarly journals Targeting lysosomal cysteine protease cathepsin S reveals immunomodulatory therapeutic strategy for oxaliplatin-induced peripheral neuropathy

Theranostics ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 4672-4687
Author(s):  
Szu-Jung Chen ◽  
Li-Hsien Chen ◽  
Yu-Min Yeh ◽  
Chou-Ching K Lin ◽  
Peng-Chan Lin ◽  
...  
Keyword(s):  
Peripheral Neuropathy ◽  
Cysteine Protease ◽  
Therapeutic Strategy ◽  
Cathepsin S ◽  
Lysosomal Cysteine Protease

scholarly journals Lysosomal cysteine protease cathepsin S is involved in cancer cell motility by regulating store-operated Ca2+ entry

2019 ◽  
Vol 1866 (12) ◽  
pp. 118517 ◽  
Author(s):  
Hsiao-Han Lin ◽  
Szu-Jung Chen ◽  
Meng-Ru Shen ◽  
Yi-Ting Huang ◽  
Hsing-Pang Hsieh ◽  
...  
Keyword(s):  
Cell Motility ◽  
Cancer Cell ◽  
Cysteine Protease ◽  
Cathepsin S ◽  
Cancer Cell Motility ◽  
Lysosomal Cysteine Protease

Immunohistochemical localization of lysosomal cysteine protease cathepsins B and L in monkey hippocampal neurons after transient ischemia

1999 ◽  
Vol 19 (3) ◽  
pp. 302-310
Author(s):  
Yukihiko Kohda ◽  
Katsuhiro Tsuchiya ◽  
Junkoh Yamashita ◽  
Masaki Yoshida ◽  
Takashi Ueno ◽  
...  
Keyword(s):  
Cysteine Protease ◽  
Hippocampal Neurons ◽  
Immunohistochemical Localization ◽  
Transient Ischemia ◽  
Lysosomal Cysteine Protease

Nonpeptidic, Noncovalent Inhibitors of the Cysteine Protease Cathepsin S

2004 ◽  
Vol 47 (20) ◽  
pp. 4799-4801 ◽  
Author(s):  
Robin L. Thurmond ◽  
Mary Pat Beavers ◽  
Hui Cai ◽  
Steven P. Meduna ◽  
Darin J. Gustin ◽  
...  
Keyword(s):  
Cysteine Protease ◽  
Cathepsin S

scholarly journals Application of a Highly Selective Cathepsin S Two-step Activity-Based Probe in Multicolor Bio-Orthogonal Correlative Light-Electron Microscopy

2021 ◽  
Vol 8 ◽  
Author(s):  
Floris J. van Dalen ◽  
Thomas Bakkum ◽  
Tyrza van Leeuwen ◽  
Mirjam Groenewold ◽  
Edgar Deu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Dendritic Cells ◽  
Immune Cell ◽  
Ultrastructural Level ◽  
Cross Reactivity ◽  
Protein Profiling ◽  
Cathepsin S ◽  
Probe Design ◽  
Competitive Activity ◽  
Lysosomal Cysteine Protease

Cathepsin S is a lysosomal cysteine protease highly expressed in immune cells such as dendritic cells, B cells and macrophages. Its functions include extracellular matrix breakdown and cleavage of cell adhesion molecules to facilitate immune cell motility, as well as cleavage of the invariant chain during maturation of major histocompatibility complex II. The identification of these diverse specific functions has brought the challenge of delineating cathepsin S activity with great spatial precision, relative to related enzymes and substrates. Here, the development of a potent and highly selective two-step activity-based probe for cathepsin S and the application in multicolor bio-orthogonal correlative light-electron microscopy is presented. LHVS, which has been reported as a selective inhibitor of cathepsin S with nanomolar potency, formed the basis for our probe design. However, in competitive activity-based protein profiling experiments LHVS showed significant cross-reactivity toward Cat L. Introduction of an azide group in the P2 position expanded the selectivity window for cathepsin S, but rendered the probe undetectable, as demonstrated in bio-orthogonal competitive activity-based protein profiling. Incorporation of an additional azide handle for click chemistry on the solvent-exposed P1 position allowed for selective labeling of cathepsin S. This highlights the influence of click handle positioning on probe efficacy. This probe was utilized in multicolor bio-orthogonal confocal and correlative light-electron microscopy to investigate the localization of cathepsin S activity at an ultrastructural level in bone marrow-derived dendritic cells. The tools developed in this study will aid the characterization of the variety of functions of cathepsin S throughout biology.

scholarly journals Mammalian legumain – A lysosomal cysteine protease with extracellular functions?

Biochimie ◽  
2019 ◽  
Vol 166 ◽  
pp. 77-83 ◽  
Author(s):  
Ngoc Nguyen Lunde ◽  
Tatjana Bosnjak ◽  
Rigmor Solberg ◽  
Harald Thidemann Johansen
Keyword(s):  
Cysteine Protease ◽  
Lysosomal Cysteine Protease

Abstract 2942: Cathepsin S inhibition alleviates oxaliplatin-induced peripheral neuropathy through regulation of Ca2+ homeostasis

2019 ◽  
Author(s):  
Szu-Jung Chen ◽  
Meng-Ru Shen ◽  
Li-Hsien Chen ◽  
Hsiao-Han Lin ◽  
Shih-Han Hsu ◽  
...  
Keyword(s):  
Peripheral Neuropathy ◽  
Cathepsin S

scholarly journals Novel Opportunities for Cathepsin S Inhibitors in Cancer Immunotherapy by Nanocarrier-Mediated Delivery

Cells ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 2021
Author(s):  
Natalie Fuchs ◽  
Mergim Meta ◽  
Detlef Schuppan ◽  
Lutz Nuhn ◽  
Tanja Schirmeister
Keyword(s):  
Therapeutic Strategy ◽  
Selective Inhibition ◽  
Cell Polarization ◽  
Matrix Proteins ◽  
Cathepsin S ◽  
Cancer Immunity ◽  
Anti Cancer ◽  
Orally Bioavailable ◽  
Antigen Presenting

Cathepsin S (CatS) is a secreted cysteine protease that cleaves certain extracellular matrix proteins, regulates antigen presentation in antigen-presenting cells (APC), and promotes M2-type macrophage and dendritic cell polarization. CatS is overexpressed in many solid cancers, and overall, it appears to promote an immune-suppressive and tumor-promoting microenvironment. While most data suggest that CatS inhibition or knockdown promotes anti-cancer immunity, cell-specific inhibition, especially in myeloid cells, appears to be important for therapeutic efficacy. This makes the design of CatS selective inhibitors and their targeting to tumor-associated M2-type macrophages (TAM) and DC an attractive therapeutic strategy compared to the use of non-selective immunosuppressive compounds or untargeted approaches. The selective inhibition of CatS can be achieved through optimized small molecule inhibitors that show good pharmacokinetic profiles and are orally bioavailable. The targeting of these inhibitors to TAM is now more feasible using nanocarriers that are functionalized for a directed delivery. This review discusses the role of CatS in the immunological tumor microenvironment and upcoming possibilities for a nanocarrier-mediated delivery of potent and selective CatS inhibitors to TAM and related APC to promote anti-tumor immunity.

Detection of cathepsin S cysteine protease in human brain tumour microdialysatesin vivo

2007 ◽  
Vol 21 (2) ◽  
pp. 204-209 ◽  
Author(s):  
T. Flannery ◽  
R. S. McConnell ◽  
S. McQuaid ◽  
G. Mcgregor ◽  
M. Mirakhur ◽  
...  
Keyword(s):  
Human Brain ◽  
Brain Tumour ◽  
Cysteine Protease ◽  
Cathepsin S

Cathepsin Q, a Novel Lysosomal Cysteine Protease Highly Expressed in Placenta

2000 ◽  
Vol 267 (3) ◽  
pp. 791-795 ◽  
Author(s):  
Katia Sol-Church ◽  
Jennifer Frenck ◽  
Robert W. Mason
Keyword(s):  
Cysteine Protease ◽  
Lysosomal Cysteine Protease
Sign in / Sign up

Export Citation Format

Share Document