scholarly journals Multiplexed non-invasive tumor imaging of glucose metabolism and receptor-ligand engagement using dark quencher FRET acceptor

Theranostics ◽  
2020 ◽  
Vol 10 (22) ◽  
pp. 10309-10325
Author(s):  
Alena Rudkouskaya ◽  
Nattawut Sinsuebphon ◽  
Marien Ochoa ◽  
Sez-Jade Chen ◽  
Joseph E. Mazurkiewicz ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Tumor Imaging ◽  
Receptor Ligand ◽  
Invasive Tumor ◽  
Non Invasive

scholarly journals Development of a Hypoxia-Selective Near-Infrared Fluorescent Probe for Non-invasive Tumor Imaging

2012 ◽  
Vol 60 (3) ◽  
pp. 402-407 ◽  
Author(s):  
Bahaa Gamal Mohamed Youssif ◽  
Kensuke Okuda ◽  
Tetsuya Kadonosono ◽  
Ola Ibrahim Abdel Razek Salem ◽  
Alaa Arafat Mohamed Hayallah ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
Tumor Imaging ◽  
Invasive Tumor ◽  
Non Invasive

scholarly journals Tc-99m Ser-Asp-Val-Glu-Cys-Gly: A novel Tc-99m labeled hexapeptide for molecular and non-invasive tumor imaging

2016 ◽  
Vol 14 (5) ◽  
pp. 4887-4892
Author(s):  
Dae-Weung Kim ◽  
Myoung Hyoun Kim ◽  
Chang Guhn Kim
Keyword(s):  
Tumor Imaging ◽  
Invasive Tumor ◽  
Non Invasive

Synthesis and evaluation of Tc-99m DTPA–glutathione as a non-invasive tumor imaging agent in a mouse colon cancer model

2014 ◽  
Vol 28 (5) ◽  
pp. 447-454 ◽  
Author(s):  
Dae-Weung Kim ◽  
Woo Hyoung Kim ◽  
Myoung Hyoun Kim ◽  
Chang Guhn Kim ◽  
Chang-Sok Oh ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Tumor Imaging ◽  
Imaging Agent ◽  
Cancer Model ◽  
Invasive Tumor ◽  
Non Invasive ◽  
Mouse Colon ◽  
Tumor Imaging Agent

scholarly journals Multiplexed Non-invasive in vivo Imaging to Assess Metabolism and Receptor Engagement in Tumor Xenografts

2019 ◽  
Author(s):  
Alena Rudkouskaya ◽  
Nattawut Sinsuebphon ◽  
Marien Ochoa ◽  
Joe E. Mazurkiewicz ◽  
Xavier Intes ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Targeted Delivery ◽  
Near Infrared ◽  
Metabolic Response ◽  
Imaging Modalities ◽  
Wide Field ◽  
Receptor Ligand ◽  
Non Invasive ◽  
Intracellular Drug Delivery

AbstractFollowing an ever-increased focus on personalized medicine, there is a continuing need to develop preclinical molecular imaging modalities to guide the development and optimization of targeted therapies. To date, non-invasive quantitative imaging modalities that can comprehensively assess simultaneous cellular drug delivery efficacy and therapeutic response are lacking. In this regard, Near-Infrared (NIR) Macroscopic Fluorescence Lifetime Förster Resonance Energy Transfer (MFLI-FRET) imaging offers a unique method to robustly quantify receptor-ligand engagement in vivo and subsequent intracellular internalization, which is critical to assess the delivery efficacy of targeted therapeutics. However, implementation of multiplexing optical imaging with FRET in vivo is challenging to achieve due to spectral crowding and cross-contamination. Herein, we report on a strategy that relies on a dark quencher that enables simultaneous assessment of receptor-ligand engagement and tumor metabolism in intact live mice. First, we establish that IRDye QC-1 (QC-1) is an effective NIR dark acceptor for the FRET-induced quenching of donor Alexa Fluor 700 (AF700) using in vitro NIR FLI microscopy and in vivo wide-field MFLI imaging. Second, we report on simultaneous in vivo imaging of the metabolic probe IRDye 800CW 2-deoxyglucose (2-DG) and MFLI-FRET imaging of NIR-labeled transferrin FRET pair (Tf-AF700/Tf-QC-1) uptake in tumors. Such multiplexed imaging revealed an inverse relationship between 2-DG uptake and Tf intracellular delivery, suggesting that 2-DG signal may predict the efficacy of intracellular targeted delivery. Overall, our methodology enables for the first time simultaneous non-invasive monitoring of intracellular drug delivery and metabolic response in preclinical studies.

scholarly journals Frequency Locked Microtoroid Optical Resonators as a Non-Invasive Tumor Biopsy Alternative

2013 ◽  
Vol 104 (2) ◽  
pp. 528a ◽  
Author(s):  
Judith Su ◽  
Alex Goldberg ◽  
Andrew Raubitschek ◽  
Brian Stoltz ◽  
Scott E. Fraser
Keyword(s):  
Optical Resonators ◽  
Tumor Biopsy ◽  
Invasive Tumor ◽  
Non Invasive

Non-invasive tumor decoding and phenotyping of cerebral gliomas utilizing multiparametric 18F-FET PET-MRI and MR Fingerprinting

2019 ◽  
Vol 47 (6) ◽  
pp. 1435-1445 ◽  
Author(s):  
Johannes Haubold ◽  
Aydin Demircioglu ◽  
Marcel Gratz ◽  
Martin Glas ◽  
Karsten Wrede ◽  
...  
Keyword(s):  
Fet Pet ◽  
Invasive Tumor ◽  
Non Invasive ◽  
Cerebral Gliomas
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