scholarly journals Donation of mitochondria by iPSC-derived mesenchymal stem cells protects retinal ganglion cells against mitochondrial complex I defect-induced degeneration

Theranostics ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 2395-2410 ◽  
Author(s):  
Dan Jiang ◽  
Guoyin Xiong ◽  
Hong Feng ◽  
Zhao Zhang ◽  
Peikai Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Retinal Ganglion Cells ◽  
Complex I ◽  
Ganglion Cells ◽  
Mitochondrial Complex I ◽  
Retinal Ganglion ◽  
Mitochondrial Complex

Treatment of traumatic optic neuropathy using human placenta-derived mesenchymal stem cells in Asian patients

2020 ◽  
Vol 15 (10) ◽  
pp. 2163-2179
Author(s):  
Youngje Sung ◽  
Sang Min Lee ◽  
Mira Park ◽  
Hye Jeong Choi ◽  
Sukho Kang ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Optic Nerve ◽  
Retinal Ganglion Cells ◽  
Optic Neuropathy ◽  
Ganglion Cells ◽  
Retinal Ganglion ◽  
Traumatic Optic Neuropathy ◽  
Asian Patients

Aim: To assess the safety and feasibility of subtenon transplantation of human placenta-derived mesenchymal stem cells (hPMSCs) in Asian patients with traumatic optic neuropathy. Materials & methods: The survival of retinal ganglion cells in the rat retina was evaluated by monitoring the expression of Tuj1 and Gfap after optic nerve compression. Based on the preclinical data, we conducted a Phase I, open label, single center, nonrandomized clinical trial in four Asian traumatic optic neuropathy patients. The safety and ophthalmologic changes were evaluated. Results: The levels of Tuj1 and Gfap expression were significantly increased in the hPMSC treatment group compared with the sham group, suggesting a protective effect of hPMSCs on the optic nerve and retinal ganglion cells. There was no evidence of adverse proliferation, tumorigenicity, severe inflammation or other serious issues during the 12-month follow-up period. Visual acuity improved in all four patients. Conclusion: The results suggested that hPMSCs are safe and have potential utility in regenerative medicine. Clinical trial registration number: 20150196587 (Korean FDA), 2015-07-123-054 (IRB).

scholarly journals Protective effects of human umbilical cord mesenchymal stem cells on retinal ganglion cells in mice with acute ocular hypertension

2021 ◽  
Vol 14 (2) ◽  
pp. 194-199
Author(s):  
Rui Liu ◽  
◽  
Hong Yang ◽  
Xiao-Yuan Sha ◽  
Guo-Cheng Yu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Protective Effect ◽  
Ocular Hypertension ◽  
Retinal Ganglion Cells ◽  
Loss Rate ◽  
Ganglion Cells ◽  
Normal Group ◽  
Human Umbilical Cord ◽  
Retinal Ganglion

AIM: To observe the protective effect of human umbilical cord mesenchymal stem cells (hucMSCs) on retinal ganglion cells (RGCs) injury in mice with acute ocular hypertension (AOH). METHODS: Fifty-six adult male C57BL/6 mice were randomly divided into four groups: normal group, AOH group, hucMSCs group, normal saline (NS) group. Left eye of mice was induced by 90 mm Hg intraocular pressure for 1h to establish AOH model. hucMSCs 1×105/µL, 1 µL or NS 1 µL was injected into the vitreous body the next day. CM-Dil fluorescent dye was used to label the 3rd generation of hucMSCs, for tracing the cells in the vitreous cavity of mice. Seven days after the model established, hematoxylin-eosin (HE) staining was used to observe the thickness of the inner retina layer in four groups. Numbers and loss rate of RGCs were evaluated by counting Brn-3a positive cells stained by immunofluorescencein. RESULTS: On the 7th day after AOH established, labeled hucMSCs were found in the vitreous cavity. HE staining showed that the thickness of retinal inner layer in AOH group was significantly lower than that in normal group and hucMSCs group (P<0.05), same as that in NS group (P>0.05). Compared with AOH group, the RGCs in normal group was significantly higher; RGCs number increased in hucMSCs group and the loss rate was lower (P<0.05). Injection of NS had no protective effect on RGCs. CONCLUSION: In AOH mouse model, vitreous injection of hucMSCs have shown a protection for RGCs.

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