scholarly journals Targeted homing of CCR2-overexpressing mesenchymal stromal cells to ischemic brain enhances post-stroke recovery partially through PRDX4-mediated blood-brain barrier preservation

Theranostics ◽  
2018 ◽  
Vol 8 (21) ◽  
pp. 5929-5944 ◽  
Author(s):  
Yinong Huang ◽  
Jiancheng Wang ◽  
Jianye Cai ◽  
Yuan Qiu ◽  
Haiqing Zheng ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Stroke Recovery ◽  
Brain Barrier ◽  
Ischemic Brain ◽  
Post Stroke ◽  
Blood Brain

Mesenchymal Stromal Cells Protect the Blood-Brain Barrier, Reduce Astrogliosis, and Prevent Cognitive and Behavioral Alterations in Surviving Septic Mice

2020 ◽  
Vol 48 (4) ◽  
pp. e290-e298 ◽  
Author(s):  
Adriano Y. O. Silva ◽  
Érica A. Amorim ◽  
Maria C. Barbosa-Silva ◽  
Maiara N. Lima ◽  
Helena A. Oliveira ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Brain Barrier ◽  
Behavioral Alterations ◽  
Blood Brain

scholarly journals Cystatin C improves blood–brain barrier integrity after ischemic brain injury in mice

2019 ◽  
Vol 153 (3) ◽  
pp. 413-425 ◽  
Author(s):  
Bo Yang ◽  
Junjie Xu ◽  
Liuhui Chang ◽  
Zhigang Miao ◽  
Dara Heang ◽  
...  
Keyword(s):  
Brain Injury ◽  
Blood Brain Barrier ◽  
Cystatin C ◽  
Brain Barrier ◽  
Ischemic Brain Injury ◽  
Ischemic Brain ◽  
Barrier Integrity ◽  
Blood Brain ◽  
Blood Brain Barrier Integrity

1335 Susceptibility to hypoxic-ischemic brain damagein neonatalrats is closely related with development of blood-brain barrier

1996 ◽  
Vol 25 ◽  
pp. S152
Author(s):  
Kanji Muramatsu ◽  
Atsuo Fukuda ◽  
Hajime Togari ◽  
Yoshiro Wada ◽  
Hitoo Nishino
Keyword(s):  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Ischemic Brain ◽  
Blood Brain

scholarly journals A Novel lncRNA ENST00000530525 Affects ANO1 Contributing to Blood-Brain Barrier Injury in Cultured hCMEC/D3 Cells Under OGD/R Conditions.

2021 ◽  
Author(s):  
Wen Jiang ◽  
Jie Li ◽  
Yuefang Cai ◽  
Wenchen Liu ◽  
Mei Chen ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Cell Viability ◽  
Blood Brain Barrier ◽  
Differentially Expressed ◽  
Brain Barrier ◽  
Cell Counting ◽  
Blood Samples ◽  
Post Stroke ◽  
Blood Brain ◽  
Acute Cerebral Ischemia

Abstract Ischemic stroke (IS) is a major neurological disease with high fatality and residual disability burdens. Increasing amount of long noncoding RNAs (lncRNAs) have been revealed to play an important role in ischemic stroke. However, the roles and significances of most lncRNAs in ischemic stroke are still unknown.This study was performed to identify differentially expressed lncRNAs using a lncRNA microarray in whole blood samples of patients suffered from acute cerebral ischemia. Bioinformatics analyses including GO, KEGG pathway enrichment analysis, and proximity to putative stroke risk location analysis were performed. A novel lncRNA ENST00000530525 significantly decreased after ischemic stroke. Furthermore, we evaluated lncRNA ENST00000530525 expression in cultured hCMEC/D3 cells under oxygen-glucose deprivation/reoxygenation(OGD/R) conditions using fluorescent in situ hybridization (FISH) and quantitative real-time polymerase chain reaction (RT-qPCR) analysis. To investigate the function of lncRNA ENST00000530525, the plasmid of overexpression(OE) and negative control(NC) were transfected into hCMEC/D3, then cell viability was detected by cell counting kit-8 (CCK-8) Assay after OGD/R,lncRNA ENST00000530525 and ANO1 expression were investigated using RT-qPCR and Immunofluorescence. For blood-brain barrier(BBB) permeability,FITC-dextran transendothelial permeability assay and Tight junction(Tj) protein was detected.There were 3352 differentially expressed lncRNAs in blood samples of acute ischemic stroke patients. The validation results were consistent with gene chip data.GO and KEGG results showed these lncRNAs were mainly related to oxygen and glucose metabolism, leukocyte transendothelial migration,mitophagy and cellular senescence.Among these, lncRNA ENST00000530525 was the highly down-regulated lncRNA and mapped within the ischemic stroke associated gene anoctamin-1 (ANO1). We furtherly found lncRNA ENST00000530525 was down-regulated in hCMEC/D3 cells under 4h OGD and 20h reoxygenation(OGD4/R20) conditions. Up-regulating lncRNA ENST00000530525 decreased the cell viability while increased ANO1 expression and contributed to BBB injury of hCMEC/D3 cells after OGD4/R20.The lncRNA ENST00000530525 might plays deleterious roles in post-stroke pathogenesis. The results show light on some differentially expressed lncRNAs in human certainly participate through characteristic roles in post-stroke pathogenesis, thus, the roles and significances of some novel lncRNAs in ischemic stroke thereby warranting further study.

scholarly journals Circulating Tight-Junction Proteins Are Potential Biomarkers for Blood-Brain Barrier Function in a Model of Neonatal Hypoxic/Ischemic Brain Injury

2020 ◽  
Author(s):  
Axel Erik Andersson ◽  
Carina Mallard ◽  
Carl Joakim Ek
Keyword(s):  
Brain Injury ◽  
Blood Brain Barrier ◽  
Brain Injuries ◽  
Brain Barrier ◽  
Neonatal Brain ◽  
Ischemic Brain ◽  
Blood Brain ◽  
Hypoxia Ischemia ◽  
The Brain ◽  
Junction Proteins

Abstract BackgroundNeonatal hypoxia-ischemia often leads to lifelong disabilities with limited treatments currently available. The brain vasculature is an important factor in many neonatal brain pathologies but there is a lack of diagnostic tools to evaluate the brain vascular health of neonates in a clinical setting. Measurement of blood-brain barrier tight-junction proteins have shown promise as biomarkers for brain injury in the adult. Here we tested the biomarker potential of tight-junctions in the context of neonatal brain injury.MethodsThe levels of TJ-proteins (occluding, claudin-5, and zonula occludens-1) in both blood plasma and cerebrospinal fluid (CSF) as well as blood-brain barrier function were measured in a clinically relevant hypoxia/ischemia model in neonatal rats.ResultsTemporally acute elevated levels of occludin and claudin-5 could be measured in blood and CSF after hypoxia/ischemia with males generally having higher levels than females. The levels of claudin-5 in CSF correlated with the severity of the brain injury at 24h post- hypoxia/ischemia. Simultaneously, we detected early increase in blood-brain barrier-permeability at 6 and 24h after hypoxia/ischemia.ConclusionsLevels of circulating claudin-5 and occludin are increased after hypoxic/ischemic brain injuries and blood-brain barrier-impairment and have promise as early biomarkers for cerebral vascular health and as a tool for risk assessment of neonatal brain injuries.

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