scholarly journals Metadata Analysis Approaches for Understanding and Improving the Functional Involvement of Rumen Microbial Consortium in Digestion and Metabolism of Plant Biomass

2019 ◽  
Vol 7 ◽  
pp. 31-45 ◽  
Author(s):  
Ayyappa Kumar Sista Kameshwar ◽  
Luiz Pereira Ramos ◽  
Wensheng Qin
Keyword(s):  
Microbial Consortium ◽  
Plant Biomass ◽  
Metadata Analysis ◽  
Functional Involvement

scholarly journals Dilution-to-Stimulation/Extinction Method: a Combination Enrichment Strategy To Develop a Minimal and Versatile Lignocellulolytic Bacterial Consortium

2020 ◽  
Vol 87 (2) ◽  
Author(s):  
Laura Díaz-García ◽  
Sixing Huang ◽  
Cathrin Spröer ◽  
Rocío Sierra-Ramírez ◽  
Boyke Bunk ◽  
...  
Keyword(s):  
Microbial Consortium ◽  
Plant Biomass ◽  
Bacterial Species ◽  
Bacterial Consortium ◽  
Priority Effects ◽  
Plant Residues ◽  
Agricultural Plant ◽  
Enrichment Strategy ◽  
Sequence Types ◽  
Bacterial Sequence

ABSTRACT The engineering of complex communities can be a successful path to understand the ecology of microbial systems and improve biotechnological processes. Here, we developed a strategy to assemble a minimal and effective lignocellulolytic microbial consortium (MELMC) using a sequential combination of dilution-to-stimulation and dilution-to-extinction approaches. The consortium was retrieved from Andean forest soil and selected through incubation in liquid medium with a mixture of three types of agricultural plant residues. After the dilution-to-stimulation phase, approximately 50 bacterial sequence types, mostly belonging to the Sphingobacteriaceae, Enterobacteriaceae, Pseudomonadaceae, and Paenibacillaceae, were significantly enriched. The dilution-to-extinction method demonstrated that only eight of the bacterial sequence types were necessary to maintain microbial growth and plant biomass consumption. After subsequent stabilization, only two bacterial species (Pseudomonas sp. and Paenibacillus sp.) became highly abundant (>99%) within the MELMC, indicating that these are the key players in degradation. Differences in the composition of bacterial communities between biological replicates indicated that selection, sampling, and/or priority effects could shape the consortium structure. The MELMC can degrade up to ∼13% of corn stover, consuming mostly its (hemi)cellulosic fraction. Tests with chromogenic substrates showed that the MELMC secretes an array of endoenzymes able to degrade xylan, arabinoxylan, carboxymethyl cellulose, and wheat straw. Additionally, the metagenomic profile inferred from the phylogenetic composition along with an analysis of carbohydrate-active enzymes of 20 bacterial genomes support the potential of the MELMC to deconstruct plant polysaccharides. This capacity was mainly attributed to the presence of Paenibacillus sp. IMPORTANCE The significance of our study mainly lies in the development of a combined top-down enrichment strategy (i.e., dilution to stimulation coupled to dilution to extinction) to build a minimal and versatile lignocellulolytic microbial consortium. We demonstrated that mainly two selectively enriched bacterial species (Pseudomonas sp. and Paenibacillus sp.) are required to drive the effective degradation of plant polymers. Our findings can guide the design of a synthetic bacterial consortium that could improve saccharification (i.e., the release of sugars from agricultural plant residues) processes in biorefineries. In addition, they can help to expand our ecological understanding of plant biomass degradation in enriched bacterial systems.

Functional Involvement of Thrombospondin in Platelet Aggregation Induced by Low Versus High Concentrations of Thrombin

1986 ◽  
Vol 55 (01) ◽  
pp. 136-142 ◽  
Author(s):  
K J Kao ◽  
David M Shaut ◽  
Paul A Klein
Keyword(s):  
Platelet Aggregation ◽  
Binding Sites ◽  
Inhibitory Effect ◽  
Activated Platelets ◽  
Low Concentrations ◽  
Platelet Binding ◽  
Functional Involvement ◽  
Thrombin Activation ◽  
Platelet Aggregates ◽  
High Concentrations

SummaryThrombospondin (TSP) is a major platelet secretory glycoprotein. Earlier studies of various investigators demonstrated that TSP is the endogenous platelet lectin and is responsible for the hemagglutinating activity expressed on formaldehyde-fixed thrombin-treated platelets. The direct effect of highly purified TSP on thrombin-induced platelet aggregation was studied. It was observed that aggregation of gel-filtered platelets induced by low concentrations of thrombin (≤0.05 U/ml) was progressively inhibited by increasing concentrations of exogenous TSP (≥60 μg/ml). However, inhibition of platelet aggregation by TSP was not observed when higher than 0.1 U/ml thrombin was used to activate platelets. To exclude the possibility that TSP inhibits platelet aggregation by affecting thrombin activation of platelets, three different approaches were utilized. First, by using a chromogenic substrate assay it was shown that TSP does not inhibit the proteolytic activity of thrombin. Second, thromboxane B2 synthesis by thrombin-stimulated platelets was not affected by exogenous TSP. Finally, electron microscopy of thrombin-induced platelet aggregates showed that platelets were activated by thrombin regardless of the presence or absence of exogenous TSP. The results indicate that high concentrations of exogenous TSP (≥60 μg/ml) directly interfere with interplatelet recognition among thrombin-activated platelets. This inhibitory effect of TSP can be neutralized by anti-TSP Fab. In addition, anti-TSP Fab directly inhibits platelet aggregation induced by a low (0.02 U/ml) but not by a high (0.1 U/ml) concentration of thrombin. In conclusion, our findings demonstrate that TSP is functionally important for platelet aggregation induced by low (≤0.05 U/ml) but not high (≥0.1 U/ml) concentrations of thrombin. High concentrations of exogenous TSP may univalently saturate all its platelet binding sites consequently interfering with TSP-crosslinking of thrombin-activated platelets.

ESTIMATION OF ABOVE GROUND BIOMASS OF THE THREE SITES (GRAZED SITE, PROTECTED SITE AND SEED SOWN SITE) FOR COMPARING THEIR PRODUCTIVITY IN KASHMIR VALLEY

2017 ◽  
Vol 23 (2) ◽  
Author(s):  
AFSHAN ANJUM BABA ◽  
SYED NASEEM UL-ZAFAR GEELANI ◽  
ISHRAT SALEEM ◽  
MOHIT HUSAIN ◽  
PERVEZ AHMAD KHAN ◽  
...  
Keyword(s):  
Protected Areas ◽  
Aboveground Biomass ◽  
Plant Biomass ◽  
Summer Season ◽  
Above Ground Biomass ◽  
Spring Season ◽  
Kashmir Valley ◽  
Ground Biomass ◽  
Maximum Value ◽  
Protected Site

The plant biomass for protected areas was maximum in summer (1221.56 g/m2) and minimum in winter (290.62 g/m2) as against grazed areas having maximum value 590.81 g/m2 in autumn and minimum 183.75 g/m2 in winter. Study revealed that at Protected site (Kanidajan) the above ground biomass ranged was from a minimum (1.11 t ha-1) in the spring season to a maximum (4.58 t ha-1) in the summer season while at Grazed site (Yousmarag), the aboveground biomass varied from a minimum (0.54 t ha-1) in the spring season to a maximum of 1.48 t ha-1 in summer seasonandat Seed sown site (Badipora), the lowest value of aboveground biomass obtained was 4.46 t ha-1 in spring while as the highest (7.98 t ha-1) was obtained in summer.

REED - PLANT BIOMASS - AS RENEWABLE AND LOW-POLLUTING ENERGY RESOURCE

2011 ◽  
Vol 10 (8) ◽  
pp. 1053-1057
Author(s):  
Gavril Budau ◽  
Mihaela Campean ◽  
Camelia Cosereanu ◽  
Dumitru Lica
Keyword(s):  
Plant Biomass ◽  
Energy Resource

МАТЕМАТИЧЕСКОЕ МОДЕЛИРОВАНИЕ ДИНАМИКИ МИНЕРАЛЬНОГО ПИТАНИЯ РАСТЕНИЙ В СИСТЕМЕ «УДОБРЕНИЕ-ПОЧВА-РАСТЕНИЕ»

2020 ◽  
Vol 65 (6) ◽  
pp. 1219-1229
Author(s):  
В.А. Четырбоцкий ◽  
◽  
А.Н. Четырбоцкий ◽  
Б.В. Левин ◽  
◽  
...  
Keyword(s):  
Experimental Data ◽  
Temporal Dynamics ◽  
Plant Biomass ◽  
Environmental Parameters ◽  
Parametric Identification ◽  
Agricultural Crop ◽  
Parameter System ◽  
Plant Nitrogen ◽  
Rhizosphere Microorganisms ◽  
Nitrogen Phosphorus

A numerical simulation of the spatial-temporal dynamics of a multi-parameter system is developed. The components of this system are plant biomass, mobile and stationary forms of mineral nutrition elements, rhizosphere microorganisms and environmental parameters (temperature, humidity, acidity). Parametric identification and verification of the adequacy of the model were carried out based on the experimental data on the growth of spring wheat «Krasnoufimskaya-100» on peat lowland soil. The results are represented by temporal distributions of biomass from agricultural crop under study and the findings on the content of main nutrition elements within the plant (nitrogen, phosphorus, potassium). An agronomic assessment and interpretation of the obtained results are given.

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