scholarly journals Genome Comparison of Pseudomonas fluorescens UM270 with Related Fluorescent Strains Unveils Genes Involved in Rhizosphere Competence and Colonization

2017 ◽  
Vol 5 ◽  
pp. 91-98 ◽  
Author(s):  
Julie E. Hernández-Salmerón ◽  
Gabriel Moreno-Hagelsieb ◽  
Gustavo Santoyo
Keyword(s):  
Pseudomonas Fluorescens ◽  
Genome Comparison ◽  
Rhizosphere Competence

scholarly journals Identification of Differences in Genome Content among phlD-Positive Pseudomonas fluorescens Strains by Using PCR-Based Subtractive Hybridization

2002 ◽  
Vol 68 (10) ◽  
pp. 5170-5176 ◽  
Author(s):  
D. V. Mavrodi ◽  
O. V. Mavrodi ◽  
B. B. McSpadden-Gardener ◽  
B. B. Landa ◽  
D. M. Weller ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Subtractive Hybridization ◽  
Open Reading Frames ◽  
Regulatory Proteins ◽  
Rhizosphere Competence ◽  
Colicin M ◽  
Genome Content ◽  
Significant Match ◽  
Reading Frames

ABSTRACT Certain 2,4-diacetylphloroglucinol-producing strains of Pseudomonas fluorescens colonize roots and suppress soilborne diseases more effectively than others from which they are otherwise phenotypically almost indistinguishable. We recovered DNA fragments present in the superior colonizer P. fluorescens Q8r1-96 but not in the less rhizosphere-competent strain Q2-87. Of the open reading frames in 32 independent Q8r1-96-specific clones, 1 was similar to colicin M from Escherichia coli, 3 resembled known regulatory proteins, and 28 had no significant match with sequences of known function. Seven clones hybridized preferentially to DNA from strains with superior rhizosphere competence, and sequences in two others were highly expressed in vitro and in the rhizosphere.

scholarly journals Involvement of Nitrate Reductase and Pyoverdine in Competitiveness of Pseudomonas fluorescens Strain C7R12 in Soil

2001 ◽  
Vol 67 (6) ◽  
pp. 2627-2635 ◽  
Author(s):  
Pascal Mirleau ◽  
Laurent Philippot ◽  
Thérèse Corberand ◽  
Philippe Lemanceau
Keyword(s):  
Nitrate Reductase ◽  
Pseudomonas Fluorescens ◽  
Type Strain ◽  
Wild Type Strain ◽  
Rhizosphere Soil ◽  
Selective Advantage ◽  
Site Directed Mutagenesis ◽  
Competitive Advantages ◽  
Wild Type ◽  
Rhizosphere Competence

ABSTRACT Involvement of nitrate reductase and pyoverdine in the competitiveness of the biocontrol strain Pseudomonas fluorescens C7R12 was determined, under gnotobiotic conditions, in two soil compartments (bulk and rhizosphere soil), with the soil being kept at two different values of matric potential (−1 and −10 kPa). Three mutants affected in the synthesis of either the nitrate reductase (Nar−), the pyoverdine (Pvd−), or both (Nar− Pvd−) were used. The Nar− and Nar− Pvd− mutants were obtained by site-directed mutagenesis of the wild-type strain and of the Pvd− mutant, respectively. The selective advantage given by nitrate reductase and pyoverdine to the wild-type strain was assessed by measuring the dynamic of each mutant-to-total-inoculant (wild-type strain plus mutant) ratio. All three mutants showed a lower competitiveness than the wild-type strain, indicating that both nitrate reductase and pyoverdine are involved in the fitness of P. fluorescens C7R12. The double mutant presented the lowest competitiveness. Overall, the competitive advantages given to C7R12 by nitrate reductase and pyoverdine were similar. However, the selective advantage given by nitrate reductase was more strongly expressed under conditions of lower aeration (−1 kPa). In contrast, the selective advantage given by nitrate reductase and pyoverdine did not differ in bulk and rhizosphere soil, indicating that these bacterial traits are not specifically involved in the rhizosphere competence but rather in the saprophytic ability of C7R12 in soil environments.

Effect of bacteriaphage and exopolysaccharide on root colonization and rhizosphere competence by Pseudomonas fluorescens

2010 ◽  
Vol 60 (2) ◽  
pp. 369-372
Author(s):  
Hong Juan Nian ◽  
Jie Zhang ◽  
Shuo Liu ◽  
Fu Ping Song ◽  
Da Fang Huang
Keyword(s):  
Pseudomonas Fluorescens ◽  
Root Colonization ◽  
Rhizosphere Competence

Exemplar Abstract for Pseudomonas fluorescens Migula 1895 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Pseudomonas Fluorescens

Exemplar Abstract for Pseudomonas fluorescens Migula 1895 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Pseudomonas Fluorescens

Nomenclature Abstract for Pseudomonas fluorescens Migula 1895 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Pseudomonas Fluorescens

Sidrophore Production and Phosphate Solubilization by Bacillus cereus and Pseudomonas fluorescens Isolated from Iraqi Soils and Soil Characterization

2018 ◽  
Vol 9 (4) ◽  
Author(s):  
Zaid Raad Abbas ◽  
Aqeel Mohammed Majeed Al-Ezee ◽  
Sawsan H
Keyword(s):  
Bacillus Cereus ◽  
Pseudomonas Fluorescens ◽  
Phosphate Solubilization ◽  
Bacterial Species ◽  
Nutrient Status ◽  
Bacterial Count ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Clear Zone ◽  
Zone Formation

This study was conducted to explore the ability of Pseudomonas fluorescens and Bacillus cereus to solubilizing a phosphate in soil for enhancing the planting growth and, its relation with soill characterization. The isolates were identified as P.fluorescens and B. cereus using convential analysis and, its phosphate solubilization ability and sidrophore was shown by the clear zone formation on National Botanical Research Institute���s Phosphate medium. Moreover, Pseudomonas fluorescens isolates (n = 9) and three of B. cereus isolated from agricultural area in Baghdad university, Mustansiriyah university and Diyala bridge. Results displayed that bacterial count were varied in soil samples according to their region, and ranging from 30 to 60 *10 2 CFU/g in Baghdad university soil to 10���20 *10 2 CFU/g in Mustansiriyah university soil, the Baghdad soil macronutrient which included: NH4, NO3, P, and K were, 8.42, 20.53, 19.09, 218.73 respectively, While the physio analysis revealed that the mean of pH was 7.3 and EC was 8.63. on the other hand the micronutrient analysis indicated that the soil samples were included Ca, Fe, Mn, Zn and Cu which gave their mean 5025.9, 8.9, 4.9, 0.5 and 1.5 respectevily. Results revealed that all isolated bacteria (9 isolates of P.fluorescens and three isolates of B. cereus gave ahalo zone which mean their ability to be phosphate solubilizing bacteria at 100%. Results revealed that all isolated bacteria were detected a ability to produce high levels from chelating agents (siderophores)) by P.fluorescens and. B cereus at 100%, when appeared ahalo clear zone. Furthermore, the high levels of phosphate solubilization and siderophore production were grouped in bacterial species isolated from Iraqi soils. might be attributed to many soil factors such as soil nutrient status, soil acidity, water content, organic matter and soil enzyme activities.

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