scholarly journals TOP2A promotes proliferation and metastasis of hepatocellular carcinoma regulated by miR-144-3p

2022 ◽  
Vol 13 (2) ◽  
pp. 589-601
Author(s):  
Tao Wang ◽  
Jing Lu ◽  
Rangrang Wang ◽  
Wanyue Cao ◽  
Junming Xu
Keyword(s):  
Hepatocellular Carcinoma ◽  
Proliferation And Metastasis

Dantrolene Potentiates the Antineoplastic Effect of Sorafenib in Hepatocellular Carcinoma via Targeting Ca+2/PI3K Signaling Pathway

2021 ◽  
Vol 14 ◽  
Author(s):  
Sherin Zakaria ◽  
Abeer Ansary ◽  
Nabil M. Abdel-Hamid ◽  
Mamdouh M. ElShishtawy
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cyclin D1 ◽  
Antineoplastic Agent ◽  
Inhibitory Effect ◽  
Pi3k Pathway ◽  
Ki 67 ◽  
Antineoplastic Effect ◽  
Pi3k Signaling Pathway ◽  
Proliferation And Metastasis

Background: Hepatocellular carcinoma (HCC) is the 6th prevalent cancer and the 4th leading cause of cancer related deaths all over the world. A major challenge for sorafenib, the standard chemotherapeutic agent in HCC treatment, is the chemo-resistance. Objective: This study was conducted to evaluate the role of dantrolene as a possible antineoplastic agent in HCC, and in chemo-sensitization of sorafenib via targeting Ca+2/PI3K pathway. Methods: HCC was induced in rats using a single dose of diethyl nitrosamine (DENA) (200 mg/kg, ip), followed by phenobarbital sodium (0.05%) in drinking water for 18 weeks. At the end of 18th week, rats were allocated into 4 groups (10 rats/each), one group was left without treatment (DENA group) and the other three groups were treated with either sorafenib, dantrolene, or their combination for further 4 weeks. One day after last injection, serum and liver tissues were collected. Liver tissue p53, VEGF, MMP-9, Cyclin D1, PI3K, and, serum AFP were assessed using immunoassay. Hepatic and serum Ca+2 were also computed. Furthermore, Ki67 was assessed immunohistochemically. Results: Dantrolene exhibited synergistic effect when used in combination with sorafenib compared to either drug alone (p <0.05) through decreasing p53, VEGF, MMP-9, Cyclin D1, and Ki-67. In addition, dantrolene was evidenced to have an inhibitory effect on Ca+2/PI3K pathway that mediates its antineoplastic action when used alone or in combination with sorafenib. Conclusion: Dantrolene exerted antineoplastic effect as well as augmented sorafenib antineoplastic activity via intervention of Ca+2/PI3K pathway, manifested by ameliorating angiogenesis, apoptosis, proliferation and metastasis.

scholarly journals LBH589 Inhibits proliferation and metastasis of hepatocellular carcinoma via inhibition of gankyrin/stat3/akt pathway

2013 ◽  
Vol 12 (1) ◽  
pp. 114 ◽  
Author(s):  
Xuan Song ◽  
Jiabei Wang ◽  
Tongsen Zheng ◽  
Ruipeng Song ◽  
Yingjian Liang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Akt Pathway ◽  
Proliferation And Metastasis

Long non-coding RNA THOR promotes cell proliferation and metastasis in hepatocellular carcinoma

Gene ◽  
2018 ◽  
Vol 678 ◽  
pp. 129-136 ◽  
Author(s):  
Zhangjun Cheng ◽  
Zhengqing Lei ◽  
Pinghua Yang ◽  
Anfeng Si ◽  
Daimin Xiang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Proliferation ◽  
Non Coding Rna ◽  
Proliferation And Metastasis ◽  
Long Non Coding Rna ◽  
Cell Proliferation And Metastasis

scholarly journals MiR-887 Promotes the Progression of Hepatocellular Carcinoma via Targeting VHL

2020 ◽  
Vol 19 ◽  
pp. 153303382094042
Author(s):  
Wei Zou ◽  
Jun Cheng
Keyword(s):  
Hepatocellular Carcinoma ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Luciferase Activity ◽  
Cell Counting ◽  
Luciferase Reporter ◽  
Chain Reaction ◽  
Gene Assay ◽  
Proliferation And Metastasis ◽  
Polymerase Chain

Background: MiR-887 has been proved to promote the tumorigenesis in diverse cancers, but its function and downstream mechanism in hepatocellular carcinoma remain obscure. Methods: Quantitative real-time polymerase chain reaction was performed to detect the expression levels of miR-887 in hepatocellular carcinoma tissues and cell lines. MiR-887 mimics and miR-887 inhibitor were transfected into Huh7 and MHCC97H to establish miR-887 overexpression or inhibition models. Cell Counting Kit-8 and colony formation experiment were conducted to monitor cell proliferation. Subcutaneous xenotransplanted tumor model and tail vein injection model in mice were also established to further verify the effect of miR-887 on hepatocellular carcinoma in vivo. The targeting relationship between miR-887 and von Hippel-Lindau tumor suppressor (VHL) was determined by quantitative real-time polymerase chain reaction, Western blot, and luciferase reporter gene assay. Results: miR-887 expression in hepatocellular carcinoma tissues was significantly upregulated. Compared with the control cells, the proliferation and metastasis of cancer cells were enhanced by miR-887 mimics and suppressed by miR-887 inhibitor. Compared with control mice, the volume and weight of subcutaneous tumors of mice in the miR-887 mimics group were significantly elevated, and the significant increase was found in the occurrence of lung metastasis. Moreover, bioinformatics tools showed that miR-887 and VHL had 2 binding sites. Luciferase activity assay demonstrated that miR-887 can inhibit the luciferase activity of VHL, and miR-887 mimics could reduce the expressions of VHL at both messenger RNA and protein levels to increase hypoxia-inducible factor α expression. Conclusion: The upregulation of miR-887 could facilitate the proliferation and metastasis of hepatocellular carcinoma cells via targeting VHL.

scholarly journals NCAPG2 overexpression promotes hepatocellular carcinoma proliferation and metastasis through activating the STAT3 and NF-κB/miR-188-3p pathways

EBioMedicine ◽  
2019 ◽  
Vol 44 ◽  
pp. 237-249 ◽  
Author(s):  
Fanzheng Meng ◽  
Shugeng Zhang ◽  
Ruipeng Song ◽  
Yao Liu ◽  
Jiabei Wang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Proliferation And Metastasis
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