scholarly journals Co-delivery of Cyclopamine and Doxorubicin Mediated by Bovine Serum Albumin Nanoparticles Reverses Doxorubicin Resistance in Breast Cancer by Down-regulating P-glycoprotein Expression

2019 ◽  
Vol 10 (10) ◽  
pp. 2357-2368 ◽  
Author(s):  
Yong-lin Lu ◽  
Ya-bin Ma ◽  
Chan Feng ◽  
Dong-lei Zhu ◽  
Jie Liu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Doxorubicin Resistance ◽  
Albumin Nanoparticles ◽  
P Glycoprotein

scholarly journals High Potency of SN-38-Loaded Bovine Serum Albumin Nanoparticles Against Triple-Negative Breast Cancer

Pharmaceutics ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 569 ◽  
Author(s):  
Lin ◽  
Chuang ◽  
Cheng ◽  
Lin ◽  
Fang
Keyword(s):  
Breast Cancer ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Triple Negative Breast Cancer ◽  
Bovine Serum ◽  
Triple Negative ◽  
Control Group ◽  
Burst Release ◽  
Albumin Nanoparticles ◽  
Low Efficiency

Triple-negative breast cancer (TNBC) is an aggressive type of breast cancer with a worse prognosis than other types. There are currently no specific approved treatments for TNBC. Albumin is a promising biomimetic material that may be fabricated into nanoparticles to possibly exert passive effects on targeted tumors. Irinotecan has been extensively used in clinical settings, although a high dosage is required due to its low efficiency of conversion into the active metabolite SN-38, also known as 7-ethyl-10-hydroxy-camptothecin. The aim of this work was to optimize SN-38-loaded bovine serum albumin nanoparticles (sBSANPs) and evaluate their potency against TNBC. The sBSANPs were characterized by a small size of about 134–264 nm, a negative charge of −37 to −40 mV, an entrapment efficiency of 59–71%, and a particle yield of 65–86%. The cytotoxicity assays using sBSANPs showed a higher potency specifically against both MDA-MB-468 and MDA-MB-231 cells (ER−, PR−, HER2−) compared to MCF-7 (ER+, PR+, HER2−), and exhibited an extremely low IC50 at the nanomolar levels (2.01–6.82 nM). The release profiles indicated that SN-38 presented an initial burst release within 12 h, and sBSANPs had a slow release pattern. Flow cytometry results showed that the fluorescence intensity of sBSANPs was significantly higher than that of the control group. The confocal images also confirmed that sBSANPs were taken up by MDA-MB-468 cells. Moreover, we found that a larger BSANP size resulted in an increased hemolytic effect. In vivo animal studies demonstrated that loading of SN-38 into bovine serum albumin nanoparticles could minimize the initial concentration without extending the elimination half-life, but significantly minimized the Cmax (p < 0.001) as compared with irinotecan treatment.

Transfecting pDNA to E . coli DH5α using bovine serum albumin nanoparticles as a delivery vehicle

Luminescence ◽  
2014 ◽  
Vol 30 (5) ◽  
pp. 583-591 ◽  
Author(s):  
Jitendra Wagh ◽  
Kuldeep J. Patel ◽  
Parth Soni ◽  
Krutika Desai ◽  
Pratik Upadhyay ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Delivery Vehicle ◽  
E Coli ◽  
Albumin Nanoparticles

Quality by Design Approach for Development and Optimization of Rifampicin Loaded Bovine Serum Albumin Nanoparticles and Characterization

2021 ◽  
Vol 18 ◽  
Author(s):  
Monica Joshi ◽  
Khushwant S. Yadav ◽  
Bala Prabhakar
Keyword(s):  
Particle Size ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Quality By Design ◽  
Entrapment Efficiency ◽  
Quality Attributes ◽  
Albumin Nanoparticles ◽  
Therapeutic Benefits ◽  
Intravenous Application

Background: Rifampicin is one of the first line drugs used for tuberculosis therapy. The therapy lasts for a long time. Thus, there is a need to develop sustained release formulation of rifampicin for intravenous application. Aim: This study is focused on preparing rifampicin loaded bovine serum albumin nanoparticles (RIF BSA NPs) suitable for intravenous application using systematic quality by design (QbD) approach. Objectives: The main objective of this study is optimizing particle size and entrapment efficiency of rifampicin loaded bovine serum albumin nanoparticles (RIF BSA NPs) and making it suitable for intravenous application using QbD approach. Methods: Quality target product profile was defined along with critical quality attributes (CQAs) for the formulation. 32 factorial design was used for achieving the predetermined values of CQAs, i.e., mean particle size <200 nm and percent entrapment efficiency>50%. Incubation time of drug with colloidal albumin solution and ratio of rifampicin: albumin, were selected as independent variables. Check point analysis was performed to confirm the suitability of regression model for optimization. Results: : The optimized RIF BSA NPs were characterized by FTIR, DSC, 1H NMR techniques. The NPs observed by transmission electron microscopy were spherical in shape. The rifampicin release could be sustained for 72 hours from BSA NPs matrix. RIF BSA NPs dispersion was stable at 5 ± 3°C for 72 hours. Non-toxicity of nanoparticles to RAW 264.7 cell line was proved by MTT assay. Conclusion: Development of RIF BSA NPs with desired quality attributes was possible by implementing QbD approach. The optimized formulation suitable for intravenous application can potentially improve the therapeutic benefits of rifampicin.

scholarly journals Improved drug targeting to liver tumor by sorafenib-loaded folate-decorated bovine serum albumin nanoparticles

Drug Delivery ◽  
2019 ◽  
Vol 26 (1) ◽  
pp. 89-97 ◽  
Author(s):  
Haipeng Wang ◽  
Shuilin Sun ◽  
Yu Zhang ◽  
Jiayi Wang ◽  
Shouhua Zhang ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Liver Tumor ◽  
Bovine Serum ◽  
Drug Targeting ◽  
Albumin Nanoparticles

Biotin-modified bovine serum albumin nanoparticles as a potential drug delivery system for paclitaxel

2019 ◽  
Vol 54 (11) ◽  
pp. 8613-8626 ◽  
Author(s):  
Danfeng Wang ◽  
Na Liang ◽  
Yoshiaki Kawashima ◽  
Fude Cui ◽  
Pengfei Yan ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Drug Delivery System ◽  
Delivery System ◽  
Bovine Serum ◽  
Potential Drug ◽  
Albumin Nanoparticles
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