scholarly journals Impact of Cold Ischemic Time and Freeze-Thaw Cycles on RNA, DNA and Protein Quality in Colorectal Cancer Tissues Biobanking

2019 ◽  
Vol 10 (20) ◽  
pp. 4978-4988
Author(s):  
Xin-Juan Fan ◽  
Yan Huang ◽  
Pei-Huang Wu ◽  
Xin-Ke Yin ◽  
Xi-Hu Yu ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Protein Quality ◽  
Ischemic Time ◽  
Freeze Thaw ◽  
Cold Ischemic Time ◽  
Cancer Tissues

scholarly journals Quantitative Assessment of Effect of Preanalytic Cold Ischemic Time on Protein Expression in Breast Cancer Tissues

2012 ◽  
Vol 104 (23) ◽  
pp. 1815-1824 ◽  
Author(s):  
V. M. Neumeister ◽  
V. Anagnostou ◽  
S. Siddiqui ◽  
A. M. England ◽  
E. R. Zarrella ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Protein Expression ◽  
Quantitative Assessment ◽  
Ischemic Time ◽  
Cold Ischemic Time ◽  
Cancer Tissues

scholarly journals Effects of Warm Ischemic Time on Gene Expression Profiling in Colorectal Cancer Tissues and Normal Mucosa

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e53406 ◽  
Author(s):  
Valeria Musella ◽  
Paolo Verderio ◽  
James Francis Reid ◽  
Sara Pizzamiglio ◽  
Manuela Gariboldi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Normal Mucosa ◽  
Ischemic Time ◽  
Warm Ischemic Time ◽  
Cancer Tissues

Mutation Detection of K-ras Gene in Paraffin-embedded Colorectal Cancer Tissues by Using Chip-based TGCE*

2010 ◽  
Vol 37 (7) ◽  
pp. 794-800 ◽  
Author(s):  
Hui-Dan ZHANG ◽  
Xiao-Nan WANG ◽  
Zhe ZHOU ◽  
Qian MA ◽  
Jin FANG
Keyword(s):  
Colorectal Cancer ◽  
Mutation Detection ◽  
Cancer Tissues ◽  
I Gene

scholarly journals LMNB2 promotes the progression of colorectal cancer by silencing p21 expression

2021 ◽  
Vol 12 (4) ◽  
Author(s):  
Chen-Hua Dong ◽  
Tao Jiang ◽  
Hang Yin ◽  
Hu Song ◽  
Yi Zhang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Cycle ◽  
Cell Proliferation ◽  
Cell Cycle Progression ◽  
Molecular Mechanisms ◽  
Gene Set Enrichment Analysis ◽  
Molecular Therapy ◽  
Cycle Progression ◽  
Cancer Tissues

AbstractColorectal cancer is the second common cause of death worldwide. Lamin B2 (LMNB2) is involved in chromatin remodeling and the rupture and reorganization of nuclear membrane during mitosis, which is necessary for eukaryotic cell proliferation. However, the role of LMNB2 in colorectal cancer (CRC) is poorly understood. This study explored the biological functions of LMNB2 in the progression of colorectal cancer and explored the possible molecular mechanisms. We found that LMNB2 was significantly upregulated in primary colorectal cancer tissues and cell lines, compared with paired non-cancerous tissues and normal colorectal epithelium. The high expression of LMNB2 in colorectal cancer tissues is significantly related to the clinicopathological characteristics of the patients and the shorter overall and disease-free cumulative survival. Functional analysis, including CCK8 cell proliferation test, EdU proliferation test, colony formation analysis, nude mouse xenograft, cell cycle, and apoptosis analysis showed that LMNB2 significantly promotes cell proliferation by promoting cell cycle progression in vivo and in vitro. In addition, gene set enrichment analysis, luciferase report analysis, and CHIP analysis showed that LMNB2 promotes cell proliferation by regulating the p21 promoter, whereas LMNB2 has no effect on cell apoptosis. In summary, these findings not only indicate that LMNB2 promotes the proliferation of colorectal cancer by regulating p21-mediated cell cycle progression, but also suggest the potential value of LMNB2 as a clinical prognostic marker and molecular therapy target.

Plexin domain containing protein 2 is more expressed within the invasive area of human colorectal cancer tissues

Human Cell ◽  
2021 ◽  
Author(s):  
Yasuhiko Hamada ◽  
Akiko Eguchi ◽  
Kyosuke Tanaka ◽  
Masaki Katsurahara ◽  
Noriyuki Horiki ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Human Colorectal Cancer ◽  
Cancer Tissues

Development of a novel RT-PCR luminometric hybridization assay for hTERT active transcript in colorectal cancer tissues

2003 ◽  
Vol 124 (4) ◽  
pp. A423
Author(s):  
K. Angelopoulou ◽  
Dimitris Dimitroulopoulos ◽  
A. Spiropoulos ◽  
A. Stthopoulou ◽  
Klisthenis Tsamakidis ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Hybridization Assay ◽  
Rt Pcr ◽  
Cancer Tissues

scholarly journals Association between R.E.N.A.L nephrometry score and perioperative outcomes following open partial nephrectomy under cold ischemia

2014 ◽  
Vol 8 (3-4) ◽  
pp. 137
Author(s):  
Dong Soo Park ◽  
Jin Ho Hwang ◽  
Moon Hyung Kang ◽  
Jong Jin Oh
Keyword(s):  
Blood Loss ◽  
Partial Nephrectomy ◽  
Perioperative Outcomes ◽  
Renal Neoplasm ◽  
Cold Ischemia ◽  
Ischemic Time ◽  
Open Partial Nephrectomy ◽  
Cold Ischemic Time ◽  
Nephrometry Score ◽  
Estimated Blood Loss

Introduction: We investigate the clinical significance of the R.E.N.A.L. nephrometry score for renal neoplasm following open partial nephrectomy (PN) under cold ischemia.Methods: A retrospective analysis was conducted using clinical data of 98 consecutive patients with clear cell renal cell carcinoma who underwent open PN by a single surgeon from December 2000 to September 2012. Tumour complexity was stratified into 3 categories: low (4-6), moderate (7-9) and high (10-12) complexity. Perioperative outcomes, such as complications, cold ischemic time, estimated blood loss and renal function, were analyzed according to the complexity by NS. Complications were stratified using the Clavien-Dindo classification system.Results: Tumour complexity according to nephrometry score was assessed as low in 16 (16.3%), moderate in 48 (49.0%) and high in 34 (34.7%). The median cold ischemic time did not differ significantly among the 3 groups (36.0 minutes in low-, 40 minutes in moderate- and 43 minutes in the high-complexity group, p = 0.421). Total complications did not differ significantly (2 (2.0%) in low, 4 (4.1%) in moderate and 4 (4.1%) in high, p = 0.984). Each Grade 3 complication occurred in the moderate (urine leakage) and high groups (lymphocele). Postoperative renal functional outcomes were similar among the groups (p = 0.729). Only mean estimated blood loss was significantly different with nephrometry score (p = 0.049).Conclusions: The nephrometry score, as used in an open PN series under cold ischemia, was not significantly associated with perioperative outcomes (i.e., ischemia time, complications, renal functional preservation).

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