scholarly journals Eukaryotic translation initiation factor 5A-2 involves in doxorubicin-induced epithelial-mesenchymal transition in oral squamous cell carcinoma cells

2018 ◽  
Vol 9 (19) ◽  
pp. 3479-3488 ◽  
Author(s):  
Liang Fang ◽  
Li Gao ◽  
Lei Xie ◽  
Guizhou Xiao
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Translation Initiation ◽  
Epithelial Mesenchymal Transition ◽  
Carcinoma Cells ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Mesenchymal Transition ◽  
Eukaryotic Translation ◽  
Eukaryotic Translation Initiation

scholarly journals N1-Guanyl-1,7-Diaminoheptane Sensitizes Estrogen Receptor Negative Breast Cancer Cells to Doxorubicin by Preventing Epithelial-Mesenchymal Transition through Inhibition of Eukaryotic Translation Initiation Factor 5A2 Activation

2015 ◽  
Vol 36 (6) ◽  
pp. 2494-2503 ◽  
Author(s):  
Yu Liu ◽  
Rongrong Liu ◽  
Peifen Fu ◽  
Feiya Du ◽  
Yun Hong ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Translation Initiation ◽  
Breast Cancer Cells ◽  
Epithelial Mesenchymal Transition ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Mesenchymal Transition ◽  
Eukaryotic Translation ◽  
Eukaryotic Translation Initiation

Background: Approximately 30% of breast cancer does not express the estrogen receptor (ER), which is necessary for endocrine-based therapy approaches. Many studies demonstrated that eukaryotic translation initiation factor 5A2 (eIF5A2) serves as a proliferation-related oncogene in tumorigenic processes. Methods: The present study used cell viability assays, EdU incorporation assays, western blot, and immunofluorescence to explore whether N1-guanyl-1,7-diaminoheptane (GC7), which inhibits eIF5A2 activation, exerts synergistic cytotoxicity with doxorubicin in breast cancer. Results: We found that GC7 enhanced doxorubicin cytotoxicity in ER-negative HCC1937 cells but had little effect in ER-positive MCF-7 and Bcap-37 cells. Administration of GC7 reversed the doxorubicin-induced epithelial-mesenchymal transition (EMT) in ER-negative breast cancer cells. Knockdown of eIF5A2 by siRNA inhibited the doxorubicin-induced EMT in ER-negative HCC1937 cells. Conclusion: These data demonstrated that GC7 combination therapy may enhance the therapeutic efficacy of doxorubicin in estrogen negative breast cancer cells by preventing EMT through inhibition of eIF5A2 activation.

scholarly journals Inhibition of miR-15a-5p Promotes the Chemoresistance to Pirarubicin in Hepatocellular Carcinoma via Targeting eIF4E

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Ying Zhang ◽  
Qingsong Tie ◽  
Zhiwei Bao ◽  
Zhi Shao ◽  
Lan Zhang
Keyword(s):  
Hepatocellular Carcinoma ◽  
Translation Initiation ◽  
Hepg2 Cells ◽  
Carcinoma Cells ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Experimental Basis ◽  
Eukaryotic Translation ◽  
Eukaryotic Translation Initiation ◽  
Direct Target

Chemoresistance has become a primary hurdle in the therapeutic outcome of hepatocellular carcinoma. Substantial evidences have demonstrated that microRNAs (miRNAs) are closely associated with the chemoresistance of hepatocellular carcinoma (HCC). Our investigation is aimed at testifying the influence of microRNA-15a-5p (miR-15a-5p)/eukaryotic translation initiation factor 4E (eIF4E) on hepatocellular carcinoma resistance to pirarubicin (THP). In our study, miR-15a-5p expression was increased in THP-treated HepG2 cells. Downregulation of miR-15a-5p blocked cell growth and elevated cell apoptosis of HepG2 cells treated with THP. Moreover, eIF4E was verified as a direct target of miR-15a-5p by binding its 3 ′ -UTR, which was confirmed by luciferase report experiment. Additionally, eIF4E was negatively associated with the miR-15a-5p expression in HepG2 cells. Mechanically, eIF4E was proven as a specific downstream of miR-15a-5p and mediated the effects of miR-15a-5p on cell viability and apoptosis of HepG2 cells treated with THP. These findings supported that miR-15a-5p facilitated THP resistance of hepatocellular carcinoma cells by modulating eIF4E, thus providing an experimental basis that miR-15a-5p might act as a novel diagnostic target in hepatocellular carcinoma resistance to THP.

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